Comparison of outflow facility before and after the microhook ab interno trabeculotomy

Eye ◽  
2021 ◽  
Author(s):  
Masaki Tanito ◽  
Aika Tsutsui ◽  
Kaoru Manabe ◽  
Mihoko Mochiji
Keyword(s):  
Outflow Facility ◽  
Before And After ◽  
Ab Interno

scholarly journals Reduction in Ocular Hypotensive Eyedrops by Ab Interno Trabeculotomy Improves Not Only Ocular Surface Condition But Also Quality of Vision

2018 ◽  
Vol 2018 ◽  
pp. 1-8 ◽  
Author(s):  
Kenji Kashiwagi ◽  
Mio Matsubara
Keyword(s):  
Visual Acuity ◽  
Ocular Surface ◽  
Surface Condition ◽  
Tear Film ◽  
Film Stability ◽  
Corneal Epithelial ◽  
Surface Conditions ◽  
Trabectome Surgery ◽  
Before And After ◽  
Ab Interno

Purpose. To investigate the effect of ab interno trabeculotomy using the Trabectome surgical system on tear film stability and functional visual acuity (FVA). Patients and Methods. Adult glaucoma patients who underwent Trabectome surgery alone or Trabectome surgery combined with phacoemulsification with intraocular lens insertion were included in this study. Corneal epithelial defects, tear film breakup time (TBUT), tear meniscus height, tear film spreading grade, tear interferometry grade, and FVA were assessed before and after surgery in addition to routine ophthalmic examinations. Changes in ocular surface conditions and visual acuity as a result of the Trabectome surgery were investigated. Results. Thirty eyes of 22 patients with a mean age of 72.2 ± 7.9 years, including 8 males and 14 females, were enrolled. The Trabectome surgery significantly reduced the intraocular pressure (IOP) from 20.3 ± 5.2 to 15.0 ± 3.3 mmHg (P<0.001) and the number of different types of ocular hypotensive eyedrops used from 3.2 ± 0.7 to 1.1 ± 0.7 types (P<0.001). The surgery significantly improved corneal epithelial defects, the tear spreading grade, the tear interferometry grade, and FVA. The surgery also improved the visual maintenance ratio among all enrolled patients, including those who underwent Trabectome surgery only. Conclusion. Trabectome surgery may be beneficial not only for IOP reduction but also for improving ocular surface conditions and FVA.

scholarly journals Intraocular Pressure Reduction in a Pigmentary Glaucoma Model by Goniotome Ab Interno Trabeculectomy

2018 ◽  
Author(s):  
Chao Wang ◽  
Yalong Dang ◽  
Priyal Shah ◽  
Hamed Esfandiari ◽  
Ying Hong ◽  
...  
Keyword(s):  
Intraocular Pressure ◽  
Ex Vivo ◽  
Pigmentary Glaucoma ◽  
Pressure Reduction ◽  
Outflow Facility ◽  
Intraocular Pressure Reduction ◽  
Ab Interno Trabeculectomy ◽  
Hematoxylin And Eosin ◽  
Anterior Segments ◽  
Ab Interno

Purpose: To investigate whether microsurgical excision of trabecular meshwork (TM) in an ex vivo pigmentary glaucoma model can normalize the hypertensive phenotype. Methods: Eight eyes of a porcine pigmentary glaucoma model underwent 90&deg; of microsurgical TM excision with an aspirating dual-blade (Goniotome (G)). 24 hours later, an additional 90&deg; of TM were removed. Anterior segments with sham surgeries served as the control (C). Outflow facility and intraocular pressure (IOP) were analyzed. Histology with hematoxylin and eosin (H&amp;E) was obtained. Results: After the first 90&deg; TM excision, IOP was significantly lower in G (10.23&plusmn;2.39 mmHg, n=7) than C (20.04&plusmn;1.97mmHg, n=8, P&lt;0.01). Outflow facility in G (0.38&plusmn;0.07 &micro;l/min/mmHg) was higher than C (0.16&plusmn;0.02 &micro;l/min/mmHg, P&lt;0.01). After the second 90&deg; TM excision, IOP in G (6.46&plusmn;0.81 mmHg, n=7) was significantly lower than C (20.25&plusmn;1.66 mmHg, n=8, P&lt;0.001), while the outflow facility in G (0.50&plusmn;0.05 &micro;l/min/mmHg, n=7) was higher than C (0.16&plusmn;0.01 &micro;l/min/mmHg, n=8, P&lt;0.001). Compared to the first excision, excision of an additional 90&deg; did not change of IOP (P=0.20) or outflow facility (P=0.17) further. Conclusion: Excision of 90&deg; of TM in a pigmentary glaucoma model using an aspirating dual-blade decreased IOP and increased outflow facility. Translational Relevance: Microsurgical TM excision over 90&deg; can effectively restore outflow in pigmentary glaucoma.

scholarly journals Outflow Enhancement by Three Different ab Interno Trabeculectomy Procedures in a Porcine Anterior Segment Model

2018 ◽  
Author(s):  
Yalong Dang ◽  
Chao Wang ◽  
Priyal Shah ◽  
Susannah Waxman ◽  
Ralitsa T. Loewen ◽  
...  
Keyword(s):  
Intraocular Pressure ◽  
Anterior Segment ◽  
Structure And Function ◽  
Outflow Facility ◽  
Segment Model ◽  
Perfusion Model ◽  
Ab Interno Trabeculectomy ◽  
Anterior Segments ◽  
And Function ◽  
Ab Interno

Objective: To evaluate three different microincisional ab interno trabeculectomy procedures in a porcine eye perfusion model. Methods: In perfused porcine anterior segments, 90 degrees of trabecular meshwork (TM) were ablated using the Trabectome (T; n = 8), Goniotome (G; n = 8), or Kahook device (K; n = 8). After 24 hours, additional 90 degrees of TM were removed. Intraocular pressure (IOP) and outflow facility were measured at 5 &micro;L/min and 10 &micro;L/min perfusion to simulate an elevated IOP. Structure and function were assessed with canalograms and histology. Results: At 5 &micro;L/min infusion rate, T resulted in a greater IOP reduction than G or K from baseline (76.12% decrease versus 48.19% and 47.96%, P = 0.013). IOP reduction between G and K was similar (P = 0.420). Removing another 90 degrees of TM caused an additional IOP reduction only in T and G but not in K. Similarly, T resulted in the largest increase in outflow facility at 5 &micro;L/min compared with G and K (first ablation: 3.41 times increase versus 1.95 and 1.87; second ablation: 4.60 versus 2.50 and 1.74) with similar results at 10 &micro;L/min (first ablation: 3.28 versus 2.29 and 1.90 (P = 0.001); second ablation: 4.10 versus 3.01 and 2.01 (P = 0.001)). Canalograms indicated circumferential flow beyond the ablation endpoints. Conclusions: T, G and K significantly increased the outflow facility. In this model, T had a larger effect than G and K.

scholarly journals Outflow Enhancement by Three Different ab Interno Trabeculectomy Procedures in a Porcine Anterior Segment Model

2018 ◽  
Author(s):  
Yalong Dang ◽  
Chao Wang ◽  
Priyal Shah ◽  
Susannah Waxman ◽  
Ralitsa T. Loewen ◽  
...  
Keyword(s):  
Intraocular Pressure ◽  
Anterior Segment ◽  
Structure And Function ◽  
Outflow Facility ◽  
Segment Model ◽  
Perfusion Model ◽  
Ab Interno Trabeculectomy ◽  
Anterior Segments ◽  
And Function ◽  
Ab Interno

Purpose: To evaluate three different microincisional ab interno trabeculectomy procedures in a porcine eye perfusion model. Methods: In perfused porcine anterior segments, 90 degrees of trabecular meshwork (TM) were ablated using the Trabectome (T; n = 8), Goniotome (G; n = 8), or Kahook device (K; n = 8). After 24 hours, additional 90 degrees of TM were removed. Intraocular pressure (IOP) and outflow facility were measured at 5 &micro;L/min and 10 &micro;L/min perfusion to simulate an elevated IOP. Structure and function were assessed with canalograms and histology. Results: At 5 &micro;L/min infusion rate, T resulted in a greater IOP reduction than G or K from baseline (76.12% decrease versus 48.19% and 47.96%, P = 0.013). IOP reduction between G and K was similar (P = 0.420). Removing another 90 degrees of TM caused an additional IOP reduction only in T and G but not in K. Similarly, T resulted in the largest increase in outflow facility at 5 &micro;L/min compared with G and K (first ablation: 3.41 times increase versus 1.95 and 1.87; second ablation: 4.60 versus 2.50 and 1.74) with similar results at 10 &micro;L/min (first ablation: 3.28 versus 2.29 and 1.90 (P = 0.001); second ablation: 4.10 versus 3.01 and 2.01 (P = 0.001)). Canalograms indicated circumferential flow beyond the ablation endpoints. Conclusions: T, G and K significantly increased the outflow facility. In this model, T had a larger effect than G and K.

scholarly journals Comparison of Different Procedures in a Combination of ab Interno Microhook Trabeculotomy and Cataract Surgery

2021 ◽  
Author(s):  
Yusaku Miura ◽  
Ken Fukuda
Keyword(s):  
Cataract Surgery ◽  
Clinical Outcomes ◽  
Case Series ◽  
Patient Characteristics ◽  
University Hospital ◽  
Retrospective Case ◽  
Before And After ◽  
The Mean ◽  
Group 2 ◽  
Ab Interno

Abstract Purpose. To compare the clinical outcomes of ab interno microhook trabeculotomy (µLOT) before and after cataract surgery for the combination of µLOT and cataract surgery. Methods. This retrospective case series included 24 eyes that underwent µLOT combined with cataract surgery at Kochi University Hospital. Groups 1 and 2 included eyes that underwent µLOT before and after cataract surgery (12 eyes each), respectively. The patient characteristics and clinical outcomes were also analyzed.Results. The mean preoperative intraocular pressure (IOP) in Groups 1 and 2 (25.3 ± 11.0 mmHg and 22.4 ± 10.5 mmHg) were reduced significantly to 14.5 ± 3.6 mmHg and 12.3 ± 3.0 mmHg, respectively, at 5–7 months postoperatively. The mean preoperative number of antiglaucoma medications in Groups 1 and 2 (4.3 ± 1.4 and 3.7 ± 1.5) had also decreased significantly to 2.7 ± 0.9 and 1.7 ± 1.6 at 5–7 months postoperatively. Postoperative hyphema with niveau formation was observed in five eyes in Group 2 (41.7%) but not in Group 1. Conclusion. For the combination of µLOT and cataract surgery, performing µLOT before cataract surgery may be less likely to result in postoperative hyphema with niveau.

Deformation Replication

1970 ◽  
Vol 28 ◽  
pp. 280-281
Author(s):  
J. Temple Black
Keyword(s):  
Cutting Speed ◽  
Machining Process ◽  
Depth Of Cut ◽  
Rake Face ◽  
Orthogonal Machining ◽  
Aluminum Chips ◽  
Before And After ◽  
Materials Used ◽  
Glass Knife ◽  
Very High

Tool materials used in ultramicrotomy are glass, developed by Latta and Hartmann (1) and diamond, introduced by Fernandez-Moran (2). While diamonds produce more good sections per knife edge than glass, they are expensive; require careful mounting and handling; and are time consuming to clean before and after usage, purchase from vendors (3-6 months waiting time), and regrind. Glass offers an easily accessible, inexpensive material ($0.04 per knife) with very high compressive strength (3) that can be employed in microtomy of metals (4) as well as biological materials. When the orthogonal machining process is being studied, glass offers additional advantages. Sections of metal or plastic can be dried down on the rake face, coated with Au-Pd, and examined directly in the SEM with no additional handling (5). Figure 1 shows aluminum chips microtomed with a 75° glass knife at a cutting speed of 1 mm/sec with a depth of cut of 1000 Å lying on the rake face of the knife.

The Clinical, Roentgenological and Morphological Effects of an Acute Exposure of Phosgene on the Lungs of Dogs

1971 ◽  
Vol 29 ◽  
pp. 236-237
Author(s):  
R. F. Bils ◽  
W. F. Diller ◽  
F. Huth
Keyword(s):  
Latent Period ◽  
Chemical Industry ◽  
Toxic Substance ◽  
Lung Edema ◽  
X Rays ◽  
Beagle Dogs ◽  
Male And Female ◽  
Morphological Alterations ◽  
Before And After ◽  
Electron Microscopes

Phosgene still plays an important role as a toxic substance in the chemical industry. Thiess (1968) recently reported observations on numerous cases of phosgene poisoning. A serious difficulty in the clinical handling of phosgene poisoning cases is a relatively long latent period, up to 12 hours, with no obvious signs of severity. At about 12 hours heavy lung edema appears suddenly, however changes can be seen in routine X-rays taken after only a few hours' exposure (Diller et al., 1969). This study was undertaken to correlate these early changes seen by the roengenologist with morphological alterations in the lungs seen in the'light and electron microscopes.Forty-two adult male and female Beagle dogs were selected for these exposure experiments. Treated animals were exposed to 94.5-107-5 ppm phosgene for 10 min. in a 15 m3 chamber. Roentgenograms were made of the thorax of each animal before and after exposure, up to 24 hrs.

Ultrastructure of Melanin Formation in Curvularia sp., Alternaria sp., and Drechslera Sorokiniana

1977 ◽  
Vol 35 ◽  
pp. 398-399
Author(s):  
M. H. Wheeler ◽  
W. J. Tolmsoff ◽  
A. A. Bell
Keyword(s):  
Potassium Phosphate ◽  
Thielaviopsis Basicola ◽  
Wild Type ◽  
Potassium Phosphate Buffer ◽  
Transmission Microscopy ◽  
Electron Transmission ◽  
Melanin Formation ◽  
Before And After ◽  
Alternaria Sp ◽  
Electron Transmission Microscopy

(+)-Scytalone [3,4-dihydro-3,6,8-trihydroxy-l-(2Hj-naphthalenone] and 1,8-di- hydroxynaphthalene (DHN) have been proposed as intermediates of melanin synthesis in the fungi Verticillium dahliae (1, 2, 3, 4) and Thielaviopsis basicola (4, 5). Scytalone is enzymatically dehydrated by V. dahliae to 1,3,8-trihydroxynaphthalene which is then reduced to (-)-vermelone [(-)-3,4- dihydro-3,8-dihydroxy-1(2H)-naphthalenone]. Vermelone is subsequently dehydrated to DHN which is enzymatically polymerized to melanin.Melanin formation in Curvularia sp., Alternaria sp., and Drechslera soro- kiniana was examined by light and electron-transmission microscopy. Wild-type isolates of each fungus were compared with albino mutants before and after treatment with 1 mM scytalone or 0.1 mM DHN in 50 mM potassium phosphate buffer, pH 7.0. Both chemicals were converted to dark pigments in the walls of hyphae and conidia of the albino mutants. The darkened cells were similar in appearance to corresponding cells of the wild types under the light microscope.

Evaluation of Freezing Methods by Low Temperature X-Ray Diffraction

1977 ◽  
Vol 35 ◽  
pp. 330-333
Author(s):  
T. Gulik-Krzywicki ◽  
M.J. Costello
Keyword(s):  
Biological Materials ◽  
Molecular Organization ◽  
X Ray Diffraction ◽  
Glass Capillary ◽  
X Ray ◽  
Rate Dependent ◽  
Before And After ◽  
Freeze Etching ◽  
Diffraction Patterns ◽  
Set Up

Freeze-etching electron microscopy is currently one of the best methods for studying molecular organization of biological materials. Its application, however, is still limited by our imprecise knowledge about the perturbations of the original organization which may occur during quenching and fracturing of the samples and during the replication of fractured surfaces. Although it is well known that the preservation of the molecular organization of biological materials is critically dependent on the rate of freezing of the samples, little information is presently available concerning the nature and the extent of freezing-rate dependent perturbations of the original organizations. In order to obtain this information, we have developed a method based on the comparison of x-ray diffraction patterns of samples before and after freezing, prior to fracturing and replication.Our experimental set-up is shown in Fig. 1. The sample to be quenched is placed on its holder which is then mounted on a small metal holder (O) fixed on a glass capillary (p), whose position is controlled by a micromanipulator.

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