scholarly journals Efficient extraction of cucurbitacins from Diplocyclos palmatus (L.) C. Jeffrey: Optimization using response surface methodology, extraction methods and study of some important bioactivities

2020 ◽  
Vol 10 (1) ◽  
Author(s):  
S. B. Patel ◽  
U. A. Attar ◽  
D. M. Sakate ◽  
S. G. Ghane
Keyword(s):  
Response Surface Methodology ◽  
Response Surface ◽  
Extraction Methods ◽  
Efficient Extraction

scholarly journals Optimasi Ekstraksi Berbantu Gelombang Ultrasonik pada Biji Melinjo Kerikil (Gnetum gnemon L., ‘Kerikil’) Menggunakan Response Surface Methodology

2019 ◽  
Vol 8 (3) ◽  
pp. 104
Author(s):  
Bambang Kunarto ◽  
Sutardi Sutardi ◽  
Supriyanto Supriyanto ◽  
Chairil Anwar
Keyword(s):  
Response Surface Methodology ◽  
Response Surface ◽  
Reducing Power ◽  
Extraction Process ◽  
Extraction Methods ◽  
Total Phenolic ◽  
Ultrasonic Bath ◽  
Assisted Extraction ◽  
Ultrasonic Assisted ◽  
Ultrasonic Assisted Extraction

Biji melinjo kerikil mengandung senyawa fenolik dan resveratrol sehingga berpotensi sebagai antioksidan, oleh karena itu perlu dilakukan ekstraksi. Metoda ekstraksi konvensional (maserasi, perebusan, refluxing) mempunyai kelemahan yaitu terjadinya kerusakan senyawa fenolik akibat reaksi oksidasi, hidrolisis dan ionisasi selama proses ekstraksi. Oleh karena itu, pada penelitian ini ekstraksi biji melinjo kerikil dilakukan menggunakan metode ekstraksi berbantu gelombang ultrasonik. Penelitian ini bertujuan untuk optimasi ekstraksi biji melinjo kerikil berbantu gelombang ultrasonik yang dilakukan pada berbagai waktu ekstraksi, suhu ekstraksi dan konsentrasi pelarut etanol. Surface response methodology digunakan untuk optimasi kondisi ekstraksi. Biji melinjo kerikil diekstrak menggunakan ultrasonic bath pada frekuensi 40 kHz dengan berbagai suhu (25, 30, 35, 40, 45oC), waktu ekstraksi (10, 20, 30, 40, 50 menit) dan konsentrasi pelarut etanol (40, 50, 60, 70 80%). Hasil penelitian menunjukkan bahwa kondisi optimum ekstraksi biji melinjo kerikil berbantu gelombang ultrasonik adalah pada suhu 30,18oC, waktu 33,01 menit dan konsentrasi pelarut etanol 71,04%. Pada kondisi ini, diperoleh yield ekstrak 18,41 ± 0,01%, total fenolik 11,26 ± 0,06 mg GAE/g, total flavonoid 533,70 ± 0,18 mg CE/100g, resveratrol 7,64 ± 0, 03%, IC50 sebesar 59,52 ± 0,04 ppm dan reducing power 76,31 ± 0,08%. Sebagai kesimpulan, optimasi ekstraksi biji melinjo kerikil berbantu gelombang ultrasonik menggunakan response surface methodology ini cukup baik karena nilai respon yang sebenarnya sesuai dengan nilai respon yang diprediksi.Melinjo kerikil seeds contain phenolic and resveratrol compounds so that it has the potential as an antioxidant, therefore extraction needs to be done. Conventional extraction methods (maceration, boiling, refluxing) have the disadvantage of devasting phenolic compounds due to oxidation, hydrolysis and ionization reactions during the extraction process. Therefore, in this study the extraction of melinjo kerikil seeds done by using the ultrasonic-assisted extraction method. The study aims to optimize ultrasonic-assisted extraction of melinjo kerikil seeds that be done at various extraction times, extraction temperatures and ethanol solvents concentrations. Response surface methodology was used to optimize experimental condition for extraction. Melinjo kerikil seeds were extracted by using ultrasonic bath at a frequency of 40 kHz with various temperatures (25, 30, 35, 40, 45oC), extraction time (10, 20, 30, 40, 50 minutes) and ethanol solvents concentrations (40, 50, 60, 70 80 %). The results showed that the optimum conditions for ultrasonic-assisted extraction of melinjo kerikil seeds were at a temperature of 30.18oC, a time of 33.01 minutes and an ethanol solvent concentration of 71.04%. In this condition, obtained the extract yield 18.41 ± 0.01%, total phenolic 11.26 ± 0.06 mg GAE/g, total flavonoids 533.70 ± 0.18 mg CE/100g, resveratrol 7.64 ± 0,03%, IC50 in the amount of 59.52 ± 0.04 ppm and reducing power 76.31 ± 0.08%. As a conclusion, the optimization ultrasonic-assisted extraction of melinjo kerikil seeds by using response surface methodology is quite good because the actual response value is in accordance with the predicted response value.

scholarly journals Optimization of Extraction Conditions for Antioxidant Activity of Acer tegmentosum Using Response Surface Methodology

2021 ◽  
Vol 11 (3) ◽  
pp. 1134
Author(s):  
Inyong Kim ◽  
Jung-Heun Ha ◽  
Yoonhwa Jeong
Keyword(s):  
Hydrogen Peroxide ◽  
Response Surface Methodology ◽  
Response Surface ◽  
Hepg2 Cells ◽  
Extraction Methods ◽  
Total Phenolic ◽  
Extraction Temperature ◽  
Freeze Dried ◽  
Hydrothermal Extraction

Extraction from edible plants is a highly important process that has various biological functions. To maximize biological activity, extraction methods should facilitate optimal extraction of functional phytochemicals. In this study, the optimal hydrothermal extraction conditions of Acer tegmentosum were determined using response surface methodology (RSM), and HepG2 cells were treated with optimized extract and hydrogen peroxide. In a central composition design, the independent variables were extraction temperature (X1: 70–90 °C), extraction time (X2: 2–6 h), and solvent-to-solid ratio (X3: 50–150). The maximum total phenolic contents (276.70 ± 10.11 mg GAE/g) and DPPH (2,2-diphenyl-1-pictylhydrazyl) activity (33.45 ± 2.20%) of A. tegmentosum were estimated at optimized extraction conditions, as follows: X1: 89.34 °C, X2: 7.36 h, X3: 184.09. Using the calculated extraction conditions, functional phytochemicals were extracted by hydrothermal extraction and freeze-dried. A. tegmentosum treatment (>10 μg/mL) of HepG2 cells remarkably attenuated hydrogen-peroxide-inducible hepatic cellular death and reactive oxygen species production in vitro.

scholarly journals Optimization of the antioxidants extraction process from the bacaba (oenocarpus distichus mart.) using response surface methodology

2020 ◽  
Vol 9 (8) ◽  
pp. e229985427
Author(s):  
Paula Beatriz Rocha Sampaio ◽  
Andressa Sousa Pereira ◽  
Caroline Roberta Freitas Pires ◽  
Rodolfo Castilho Clemente ◽  
Guilherme Nobre Lima do Nascimento
Keyword(s):  
Antioxidant Activity ◽  
Response Surface Methodology ◽  
Rural Communities ◽  
Response Surface ◽  
Total Phenolics ◽  
Extraction Process ◽  
Solvent Mixture ◽  
Chemical Characteristics ◽  
Palm Trees ◽  
Efficient Extraction

Bacaba (Oenocarpus distichus Mart) is a fruit that comes from palm trees native from the Arecaceae family and presents important nutritional and socioeconomic value for rural communities. Although being very popular, this fruit has very few cataloged chemical characteristics. The present study aimed to optimize the bacaba antioxidant extraction process by investigating the most efficient extraction interval and the best rotation time, using ethanol and methanol/acetone solvents, with the content of total phenolics and antioxidant activity. Methanol acetone solvent mixture was the most efficient solvent for extracting antioxidants from bacaba, with an extraction time of 90 minutes and 20 minutes of rotation interval at 5,000 rpm.

scholarly journals Optimization of reflux extraction for cat’s whiskers leaves extract using response surface methodology

2020 ◽  
Vol 26 (1) ◽  
pp. 49-57
Author(s):  
Mohd Ariff ◽  
Norfazilah Abdullah
Keyword(s):  
Antioxidant Activity ◽  
Response Surface Methodology ◽  
Response Surface ◽  
Radical Scavenging Activity ◽  
Radical Scavenging ◽  
Extraction Methods ◽  
Solvent Ratio ◽  
Operation Conditions ◽  
Central Composite Experimental Design ◽  
Cat’S Whiskers

Cat?s whiskers (Orthosiphon stamineus) is a medicinal plant which comprises several dynamic pharmacological properties such as anti-inflammatory, antioxidant and antibacterial. In small and medium-scale industries, conventional reflux extraction method is favored as compared to other non-conventional extraction methods due to cost effectiveness and simple operating procedures. In this study, response surface methodology (RSM) was applied to optimize the reflux conditions for extraction of cat?s whiskers leaves in order to achieve a high content of antioxidant activity in the extracts. Central composite experimental design (CCD) with three factors and three levels was employed to consider the effects of the operation conditions. Antioxidant activity of the extracts were based on free radical scavenging activity (DPPH assay) and were analyzed using a UV-Vis spectrophotometer. Based on RSM, the antioxidant activity could be maximized when the operation conditions were 125 ?m for particle size, 1.5:20 for sample-to-solvent ratio, and 2 h for extraction time. Under these optimal conditions, the predicted value of the antioxidant activity was compared with the actual, and the mean error was 0.46%. This indicates the suitability of the model for optimizing the conditions for the reflux extraction of cat?s whiskers leaves.

Optimization of Subcritical 1,1,1,2-Tetrafluoroethane( R134a ) Removal of Cholesterol from Spray-Dried Sthenoteuthis oualaniensis Egg Powder Using Response Surface Methodology

2014 ◽  
Vol 1033-1034 ◽  
pp. 717-723 ◽  
Author(s):  
Xiao Sui ◽  
Xiao Mei Feng ◽  
Rong Yan Yue ◽  
Yu Qian Han ◽  
Chang Hu Xue
Keyword(s):  
Response Surface Methodology ◽  
Response Surface ◽  
Removal Rate ◽  
Extraction Methods ◽  
Negative Effects ◽  
Optimum Parameters ◽  
Sthenoteuthis Oualaniensis ◽  
Egg Powder ◽  
C 50 ◽  
Spray Dried

Subcritical 1, 1, 1, 2-tetrafluoroethane (R134a) extraction was applied to remove cholesterol from spray-dried Sthenoteuthis oualaniensis egg powder. Response surface methodology (RSM) was applied to optimize the parameters of subcritical R134a extraction (pressure, temperature, and extraction time) on removal rate of cholesterol. Response surface analysis showed that the data came to a precise fitting to a second-order polynomial model. The quadratic terms of pressure and temperature had high significant negative effects (p＜0.001) on removal rate. The optimum parameters of the variables were 8.6 MPa, 55.4 °C, 50 min. Under these conditions, removal rate of cholesterol was predicted to be 101% and experiments gave out the removal rate of 99.16%, which concurred with the model prediction. Furthermore, the removal efficiency of cholesterol by R134a was higher than those achieved by using supercritical carbon dioxide(SC-CO2)and β-cyclodextrin extraction methods.

Response Surface Methodology Optimization for Extraction of Natural Anthocyanins from Vietnamese Carissa сarandas L. Fruit

2019 ◽  
Vol 814 ◽  
pp. 475-480
Author(s):  
Tri Nhut Pham ◽  
Thi Ha Xuan Huynh ◽  
Bui Phuc Tran ◽  
Thien Hien Tran ◽  
Phu Thuong Nhan Nguyen ◽  
...  
Keyword(s):  
Response Surface Methodology ◽  
Response Surface ◽  
Fruits And Vegetables ◽  
Extraction Methods ◽  
Extraction Time ◽  
Extraction Temperature ◽  
Box Behnken Design ◽  
Solid Ratio ◽  
Naturally Occurring ◽  
Carissa Carandas

Anthocyanins are naturally occurring compounds that are responsible for a wide variety of colors in many plants, fruits and vegetables. In this study, the extraction of natural anthocyanins from Vietnamese Carissa carandas L. beverage was optimized using response surface methodology (RSM). We applied a Box–Behnken design consisting of three levels and three factors. Examined factors are extraction temperature (ranging from 40 to 60°C), liquid to solid ratio (ranging from 2:1 to 4:1), extraction time (ranging from 30 to 60 min). Using 60% ethanol as solvent for the process, we determined the maximum yields of anthocyanin was 273.786 mg/L. This yield corresponds to extraction conditions of 3:1 (v/w) liquid to solid ratio, temperature of 48.10 °C with a 44.08 min extraction time. The experimental results also fit well with the proposed response model of anthocyanin yield (R2 = 0.9992). Therefore, this study suggested optimization of different extraction methods for the defatted fruit parts.

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