scholarly journals Genome analysis provides insight into hyper-virulence of Streptococcus suis LSM178, a human strain with a novel sequence type 1005

2021 ◽  
Vol 11 (1) ◽  
Author(s):  
Yong Hu ◽  
Shiming Fu ◽  
Geng Zou ◽  
Anusak Kerdsin ◽  
Xiabing Chen ◽  
...  
Keyword(s):  
Virulent Strain ◽  
Streptococcus Suis ◽  
Sequence Type ◽  
Type I ◽  
Adaptive Potential ◽  
The Public ◽  
Virulence Regulation ◽  
Virulent Strains ◽  
Highly Virulent ◽  
Insight Into

AbstractStreptococcus suis has been well-recognized as a zoonotic pathogen worldwide, and the diversity and unpredictable adaptive potential of sporadic human strains represent a great risk to the public health. In this study, S. suis LSM178, isolated from a patient in contact with pigs and raw pork, was assessed as a hyper-virulent strain and interpreted for the virulence based on its genetic information. The strain was more invasive for Caco-2 cells than two other S. suis strains, SC19 and P1/7. Sequence analysis designated LSM178 with serotype 2 and a novel sequence type 1005. Phylogenetic analysis showed that LSM178 clustered with highly virulent strains including all human strains and epidemic strains. Compared with other strains, these S. suis have the most and the same virulent factors and a type I-89 K pathogenicity island. Further, groups of genes were identified to distinguish these highly virulent strains from other generally virulent strains, emphasizing the key roles of genes modeling transcription, cell barrier, replication, recombination and repair on virulence regulation. Additionally, LSM178 contains a novel prophage conducive potentially to pathogenicity.

scholarly journals Whole-Genome-Sequence of Streptococcus Suis LSM178 with a Novel ST1005 Characterized the Hyper Pathogenicity in Human Infection

2021 ◽  
Author(s):  
Yong Hu ◽  
Shiming Fu ◽  
Xingxing Dong ◽  
Lin Teng ◽  
Jinquan Li
Keyword(s):  
Genetic Information ◽  
Streptococcus Suis ◽  
Human Infection ◽  
Sequence Type ◽  
Whole Genome Sequence ◽  
Type I ◽  
Zoonotic Pathogen ◽  
The Public ◽  
Virulent Strains ◽  
Highly Virulent

Abstract Streptococcus suis (S. suis) has been well-recognized as a zoonotic pathogen worldwide gearing up a great risk to the public health. In this study, an S. suis LSM178 strain with serotype 2 and novel multi-locus sequence type of 1005, isolating from a patient, was interpreted for the pathogenicity by its genetic information. LSM178 was more efficiently invasive to Caco-2 cells than SC19 and P1/7. Phylogenetic analysis showed that LSM178 clustered with highly virulent strains including all human strains and epidemic strains. These serotype 2 S. suis from China shared exclusively the typical virulence characteristics including the maximum (95/96) virulent factors and type I-89 K Pathogenicity Island. Further, groups of genes were identified to distinguish these highly virulent strains from other generally virulent strains, emphasizing the key roles of genes modeling transcription, cell barrier, replication, recombination and repair on the high pathogenicity for highly virulent strains. Additionally, LSM178 contains a novel prophage conducive potentially to pathogenicity. These characters would contribute to deeply studying the pathogenic mechanism and virulence drift of human pathogenic S. suis.

scholarly journals Label-Free Quantitative Acetylome Analysis Reveals Toxoplasma gondii Genotype-Specific Acetylomic Signatures

2019 ◽  
Vol 7 (11) ◽  
pp. 510 ◽  
Author(s):  
Ze-Xiang Wang ◽  
Rui-Si Hu ◽  
Chun-Xue Zhou ◽  
Jun-Jun He ◽  
Hany M. Elsheikha ◽  
...  
Keyword(s):  
Toxoplasma Gondii ◽  
Label Free ◽  
Histone Acetyl Transferase ◽  
Genotype I ◽  
Host Cell Response ◽  
Genotype Iii ◽  
Virulent Strains ◽  
Highly Virulent ◽  
Insight Into ◽  
Specific Virulence

Distinct genotypic and pathogenic differences exist between Toxoplasma gondii genotypes. For example, genotype I is highly virulent, whereas genotype II and genotype III are less virulent. Moreover, Chinese 1 genotype (ToxoDB#9) is also virulent. Here, we compare the acetylomes of genotype 1 (RH strain) and Chinese 1 genotype (ToxoDB#9, PYS strain) of T. gondii. Using mass spectrometry enriched for acetylated peptides, we found a relationship between the levels of protein acetylation and parasite genotype-specific virulence. Notably, lysine acetylation was the largest (458 acetylated proteins) in RH strain, followed by PYS strain (188 acetylated proteins), whereas only 115 acetylated proteins were detected in PRU strain. Our analysis revealed four, three, and four motifs in RH strain, PRU strain and PYS strain, respectively. Three conserved sequences around acetylation sites, namely, xxxxxKAcHxxxx, xxxxxKAcFxxxx, and xxxxGKAcSxxxx, were detected in the acetylome of the three strains. However, xxxxxKAcNxxxx (asparagine) was found in RH and PYS strains but was absent in PRU strain. Our analysis also identified 15, 3, and 26 differentially expressed acetylated proteins in RH strain vs. PRU strain, PRU strain vs. PYS strain and PYS strain vs. RH strain, respectively. KEGG pathway analysis showed that a large proportion of the acetylated proteins are involved in metabolic processes. Pathways for the biosynthesis of secondary metabolites, biosynthesis of antibiotics and microbial metabolism in diverse environments were featured in the top five enriched pathways in all three strains. However, acetylated proteins from the virulent strains (RH and PYS) were more enriched in the pyruvate metabolism pathway compared to acetylated proteins from PRU strain. Increased levels of histone-acetyl-transferase and glycyl-tRNA synthase were detected in RH strain compared to PRU strain and PYS strain. Both enzymes play roles in stress tolerance and proliferation, key features in the parasite virulence. These findings reveal novel insight into the acetylomic profiles of major T. gondii genotypes and provide a new important resource for further investigations of the roles of the acetylated parasite proteins in the modulation of the host cell response to the infection of T. gondii.

scholarly journals Identification of an Inducible Bacteriophage in a Virulent Strain of Streptococcus suis Serotype 2

2003 ◽  
Vol 71 (10) ◽  
pp. 6104-6108 ◽  
Author(s):  
J. Harel ◽  
G. Martinez ◽  
A. Nassar ◽  
H. Dezfulian ◽  
S. J. Labrie ◽  
...  
Keyword(s):  
Dna Sequences ◽  
Virulent Strain ◽  
Streptococcus Suis ◽  
Swine Industry ◽  
Subtraction Hybridization ◽  
Gram Positive Bacteria ◽  
Virulence Markers ◽  
Virulent Strains ◽  
Phage Dna ◽  
Suis Serotype

ABSTRACT Streptococcus suis infection is considered to be a major problem in the swine industry worldwide. Most virulent Canadian isolates of S. suis serotype 2 do not produce the known virulence markers for this pathogen. PCR-based subtraction hybridization was adapted to isolate unique DNA sequences which were specific to virulent strains of S. suis isolated in Canada. Analysis of some subtracted DNA clones revealed significant homology with bacteriophages of gram-positive bacteria. An inducible phage (named Ss1) was observed in S. suis following the incubation of the virulent strain 89-999 with mitomycin C. Phage Ss1 has a long noncontractile tail and a small isometric nucleocapsid and is a member of the Siphoviridae family. Ss1 phage DNA appears to be present in most Canadian S. suis strains tested in this study, which were isolated from diseased pigs or had proven virulence in mouse or pig models. To our knowledge, this is the first report of the isolation of a phage in S. suis.

scholarly journals Draft Genome Sequence of Streptococcus suis S10, a Virulent Strain Used in Experimental Pig Infections

2019 ◽  
Vol 8 (23) ◽  
Author(s):  
Rogier A. Gaiser ◽  
Aldert L. Zomer ◽  
Jerry M. Wells ◽  
Peter van Baarlen
Keyword(s):  
Infectious Diseases ◽  
Genome Sequence ◽  
Virulent Strain ◽  
Draft Genome ◽  
Streptococcus Suis ◽  
Draft Genome Sequence ◽  
Whole Genome Sequence ◽  
Whole Genome ◽  
Content Type ◽  
Highly Virulent

Here, we report the draft whole-genome sequence of Streptococcus suis strain S10, isolated from the tonsils of a healthy pig. S. suis S10 belongs to the highly virulent serotype 2, which includes isolates that cause infectious diseases, including meningitis, in pigs and human.

scholarly journals Prevalent distribution and conservation of Streptococcus suis Lmb protein and its protective capacity against the Chinese highly virulent strain infection

2014 ◽  
Vol 169 (5-6) ◽  
pp. 395-401 ◽  
Author(s):  
Yan-Mei Zhang ◽  
Zhu-Qing Shao ◽  
Jing Wang ◽  
Ling Wang ◽  
Xianfu Li ◽  
...  
Keyword(s):  
Virulent Strain ◽  
Streptococcus Suis ◽  
Protective Capacity ◽  
Highly Virulent

scholarly journals Pepper Accession LS2341 Is Highly Resistant to Ralstonia solanacearum Strains from Japan

HortScience ◽  
2009 ◽  
Vol 44 (7) ◽  
pp. 2038-2040 ◽  
Author(s):  
Yutaka Mimura ◽  
Masami Yoshikawa ◽  
Masashi Hirai
Keyword(s):  
Ralstonia Solanacearum ◽  
Host Plants ◽  
Virulent Strain ◽  
Resistant Cultivars ◽  
Potential Source ◽  
Virulent Strains ◽  
Resistant Lines ◽  
Tropical Areas ◽  
Warm Temperate ◽  
Highly Virulent

Bacterial wilt caused by Ralstonia solanacearum is one of the most serious diseases in pepper (Capsicum annuum) crops in warm-temperate, subtropical, and tropical areas, including Japan. Resistant lines are a prerequisite for breeding resistant cultivars but are not well studied. Eight pepper accessions previously described as resistant to the pathogen were selected and inoculated with a highly virulent strain, KP9547. Among them, Malaysian accession LS2341 exhibited the highest resistance. Accession LS2341 was then challenged with 14 virulent strains collected from various areas in Japan. The strains cover biovars 2T, 3, and 4 and include isolates from the host plants of tomato, eggplant, and pepper. Results indicated that accession LS2341 had the highest level of resistance to all the strains of R. solanacearum examined. Therefore, accession LS2341 was confirmed as an appropriate source for the breeding of resistant cultivars of pepper in Japan and is a candidate for a potential source of resistance in other areas.

scholarly journals Complete Genome Sequences of Canadian Epidemic Methicillin-Resistant Staphylococcus aureus Strains CMRSA3 and CMRSA6

2018 ◽  
Vol 7 (5) ◽  
Author(s):  
Jo-Ann McClure ◽  
Steven M. Shideler ◽  
Kunyan Zhang
Keyword(s):  
Staphylococcus Aureus ◽  
Methicillin Resistant Staphylococcus Aureus ◽  
Clonal Complex ◽  
Virulent Strain ◽  
Sequence Type ◽  
Genome Sequences ◽  
Methicillin Resistant ◽  
Content Type ◽  
Mrsa Strains ◽  
Highly Virulent

Methicillin-resistant Staphylococcus aureus (MRSA) clonal complex 8 (CC8) sequence type 239 (ST239) represents a predominant hospital-associated MRSA sublineage present worldwide. The Canadian epidemic MRSA strains CMRSA3 and CMRSA6 are moderately virulent members of this group but are closely related to the highly virulent strain TW20.

scholarly journals Hybrid Pathogenicity Island PAGI-5 Contributes to the Highly Virulent Phenotype of a Pseudomonas aeruginosa Isolate in Mammals

2008 ◽  
Vol 190 (21) ◽  
pp. 7130-7140 ◽  
Author(s):  
Scott E. Battle ◽  
Folker Meyer ◽  
Jordi Rello ◽  
Vanderlene L. Kung ◽  
Alan R. Hauser
Keyword(s):  
Pseudomonas Aeruginosa ◽  
Virulent Strain ◽  
Subtractive Hybridization ◽  
Genomic Islands ◽  
Pathogenicity Islands ◽  
Virulence Determinants ◽  
Virulent Strains ◽  
Virulent Phenotype ◽  
Models Of Disease ◽  
Highly Virulent

ABSTRACT Most known virulence determinants of Pseudomonas aeruginosa are remarkably conserved in this bacterium's core genome, yet individual strains differ significantly in virulence. One explanation for this discrepancy is that pathogenicity islands, regions of DNA found in some strains but not in others, contribute to the overall virulence of P. aeruginosa. Here we employed a strategy in which the virulence of a panel of P. aeruginosa isolates was tested in mouse and plant models of disease, and a highly virulent isolate, PSE9, was chosen for comparison by subtractive hybridization to a less virulent strain, PAO1. The resulting subtractive hybridization sequences were used as tags to identify genomic islands found in PSE9 but absent in PAO1. One 99-kb island, designated P. aeruginosa genomic island 5 (PAGI-5), was a hybrid of the known P. aeruginosa island PAPI-1 and novel sequences. Whereas the PAPI-1-like sequences were found in most tested isolates, the novel sequences were found only in the most virulent isolates. Deletional analysis confirmed that some of these novel sequences contributed to the highly virulent phenotype of PSE9. These results indicate that targeting highly virulent strains of P. aeruginosa may be a useful strategy for identifying pathogenicity islands and novel virulence determinants.

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