scholarly journals Effectiveness assessment of using riverine water eDNA to simultaneously monitor the riverine and riparian biodiversity information

2021 ◽  
Vol 11 (1) ◽  
Author(s):  
Haile Yang ◽  
Hao Du ◽  
Hongfang Qi ◽  
Luxian Yu ◽  
Xindong Hou ◽  
...  
Keyword(s):  
Reference Method ◽  
Environmental Dna ◽  
Weather Conditions ◽  
Tibet Plateau ◽  
Riverine Water ◽  
Terrestrial Biodiversity ◽  
Bacterial Biodiversity ◽  
Effectiveness Assessment ◽  
Biodiversity Information ◽  
Rainy Days

AbstractBoth aquatic and terrestrial biodiversity information can be detected in riverine water environmental DNA (eDNA). However, the effectiveness of using riverine water eDNA to simultaneously monitor the riverine and terrestrial biodiversity information remains unidentified. Here, we proposed that the monitoring effectiveness could be approximated by the transportation effectiveness of land-to-river and upstream-to-downstream biodiversity information flows and described by three new indicators. Subsequently, we conducted a case study in a watershed on the Qinghai–Tibet Plateau. The results demonstrated that there was higher monitoring effectiveness on summer or autumn rainy days than in other seasons and weather conditions. The monitoring of the bacterial biodiversity information was more efficient than the monitoring of the eukaryotic biodiversity information. On summer rainy days, 43–76% of species information in riparian sites could be detected in adjacent riverine water eDNA samples, 92–99% of species information in riverine sites could be detected in a 1-km downstream eDNA sample, and half of dead bioinformation (the bioinformation labeling the biological material that lacked life activity and fertility) could be monitored 4–6 km downstream for eukaryotes and 13–19 km downstream for bacteria. The current study provided reference method and data for future monitoring projects design and for future monitoring results evaluation.

scholarly journals Effectiveness Assessment of Using Riverine Water eDNA to Simultaneously Monitor the Riverine and Riparian Biodiversity Information

2021 ◽  
Author(s):  
Haile Yang ◽  
Hao Du ◽  
Hongfang Qi ◽  
Luxian Yu ◽  
Xindong Hou ◽  
...  
Keyword(s):  
Reference Method ◽  
Environmental Dna ◽  
Weather Conditions ◽  
Tibet Plateau ◽  
Riverine Water ◽  
Terrestrial Biodiversity ◽  
Bacterial Biodiversity ◽  
Effectiveness Assessment ◽  
Biodiversity Information ◽  
Rainy Days

Abstract Both aquatic and terrestrial biodiversity information can be detected in riverine water environmental DNA (eDNA). However, the effectiveness of using riverine water eDNA to simultaneously monitor the riverine and terrestrial biodiversity information remains unidentified. Here, we proposed that the monitoring effectiveness could be approximated by the transportation effectiveness of land-to-river and upstream-to-downstream biodiversity information flows and described by three new indicators. Subsequently, we conducted a case study in a watershed on the Qinghai-Tibet Plateau. The results demonstrated that there was higher monitoring effectiveness on summer or autumn rainy days than in other seasons and weather conditions. The monitoring of the bacterial biodiversity information was more efficient than the monitoring of the eukaryotic biodiversity information. On summer rainy days, 43-76% of species information in riparian sites could be detected in water eDNA samples, 92-99% of species information in riverine sites could be detected in a 1-km downstream eDNA sample, and half of dead bioinformation (i.e., the bioinformation labeling the biological material that lacked life activity and fertility) could be monitored 4-6 km downstream for eukaryotes and 13-19 km downstream for bacteria. The current study provided reference method and data for future monitoring projects design and for future monitoring results evaluation.

scholarly journals Simultaneously monitoring aquatic and terrestrial biodiversity using riverine water eDNA: seasonal variation of monitoring effectiveness

2020 ◽  
Author(s):  
Haile Yang ◽  
Hao Du ◽  
Hongfang Qi ◽  
Luxian Yu ◽  
Xindong Hou ◽  
...  
Keyword(s):  
Environmental Dna ◽  
Operational Taxonomic Unit ◽  
Climatic Conditions ◽  
Biological Information ◽  
Tibet Plateau ◽  
Riverine Water ◽  
Terrestrial Biodiversity ◽  
Effectiveness Assessment ◽  
Qinghai Tibet Plateau ◽  
Biodiversity Information

AbstractBoth aquatic and terrestrial biodiversity information can be detected in riverine water environmental DNA (eDNA). However, the monitoring effectiveness (i.e., the proportion of aquatic and terrestrial biodiversity information detected in riverine water eDNA samples) is unknown. To investigate the monitoring effectiveness, we introduced the concept of watershed biological information flow (WBIF) and proposed that the monitoring effectiveness depended on the transportation effectiveness of the WBIF. Then, the monitoring effectiveness could be assessed in the WBIF framework. Here, we conducted a monitoring effectiveness assessment case study in a watershed on the Qinghai-Tibet Plateau according to analysis of the bacterial operational taxonomic unit (OTU) assemblages detected in riverine water eDNA samples and riparian soil eDNA samples during three seasons. The results showed that (1) the downstream-to-upstream monitoring effectiveness: only 76% of the bacterial OTUs could be detected 1 km downstream in spring and more than 97% and 96% could be detected in summer and autumn, respectively. (2) The river-to-land monitoring effectiveness: more than 62% of the bacterial OTUs in riparian soil eDNA samples could be detected in adjacent riverine water eDNA samples on rainy summer days and 16% and 48% could be detected on cloudy spring and autumn days, respectively. These results suggested that riverine water eDNA was viable for simultaneously monitoring aquatic and terrestrial biodiversity and that rainy days in summer or autumn were suitable sampling times on the Qinghai-Tibet Plateau. More studies on monitoring effectiveness in other taxonomies and in other watersheds with different climatic conditions are needed to support simultaneous aquatic and terrestrial biodiversity assessments.

scholarly journals Uncertainty analysis of eddy covariance CO<sub>2</sub> flux measurements for different EC tower distances using an extended two-tower approach

2015 ◽  
Vol 12 (4) ◽  
pp. 1205-1221 ◽  
Author(s):  
H. Post ◽  
H. J. Hendricks Franssen ◽  
A. Graf ◽  
M. Schmidt ◽  
H. Vereecken
Keyword(s):  
Eddy Covariance ◽  
Reference Method ◽  
Co2 Flux ◽  
Weather Conditions ◽  
Data Set ◽  
Random Uncertainty ◽  
Extended Approach ◽  
Uncertainty Estimates ◽  
Flux Measurements

Abstract. The use of eddy covariance (EC) CO2 flux measurements in data assimilation and other applications requires an estimate of the random uncertainty. In previous studies, the (classical) two-tower approach has yielded robust uncertainty estimates, but care must be taken to meet the often competing requirements of statistical independence (non-overlapping footprints) and ecosystem homogeneity when choosing an appropriate tower distance. The role of the tower distance was investigated with help of a roving station separated between 8 m and 34 km from a permanent EC grassland station. Random uncertainty was estimated for five separation distances with the classical two-tower approach and an extended approach which removed systematic differences of CO2 fluxes measured at two EC towers. This analysis was made for a data set where (i) only similar weather conditions at the two sites were included, and (ii) an unfiltered one. The extended approach, applied to weather-filtered data for separation distances of 95 and 173 m gave uncertainty estimates in best correspondence with an independent reference method. The introduced correction for systematic flux differences considerably reduced the overestimation of the two-tower based uncertainty of net CO2 flux measurements and decreased the sensitivity of results to tower distance. We therefore conclude that corrections for systematic flux differences (e.g., caused by different environmental conditions at both EC towers) can help to apply the two-tower approach to more site pairs with less ideal conditions.

scholarly journals Association among Weather Conditions, Ambient Air Temperature, and Sedentary Time in Chinese Adults

2019 ◽  
Vol 2019 ◽  
pp. 1-6
Author(s):  
Xu Wen ◽  
Yiqun Ma ◽  
Bing Yuan ◽  
Fubaihui Wang
Keyword(s):  
Air Temperature ◽  
Sedentary Time ◽  
Meteorological Data ◽  
Weather Condition ◽  
Weather Conditions ◽  
Ambient Air ◽  
Chinese Adults ◽  
Ambient Air Temperature ◽  
Meteorological Observatory ◽  
Rainy Days

This study is aimed to quantify the association among weather conditions, ambient air temperature, and sedentary time in Chinese adults. The participants were 3,270 Chinese users of a wrist-worn activity tracker. Their daily activity data were collected using an algorithm based on raw data to determine the sedentary time. The data of ambient air temperature and weather were collected from the meteorological data released by China Central Meteorological Observatory. Two-level linear regression analyses showed that weather conditions had a significant influence on sedentary time in Chinese adults after adjustments for some covariates were made. When the weather condition changed from rainy days to sunny and cloudy days, sedentary time might decrease by about 6.89 and 5.60 min, respectively. In conclusion, weather conditions were independently associated with sedentary time in Chinese adults. The daily sedentary time was shorter on sunny and cloudy days than on rainy days.

scholarly journals Environmental DNA reveals that rivers are conveyer belts of biodiversity information

2015 ◽  
Author(s):  
Kristy Deiner ◽  
Emanuel A. Fronhofer ◽  
Elvira Meächler ◽  
Jean-Claude Walser ◽  
Florian Altermatt
Keyword(s):  
Spatial Autocorrelation ◽  
Empirical Data ◽  
Spatial Scales ◽  
Environmental Dna ◽  
River Networks ◽  
Water Interface ◽  
Biodiversity Data ◽  
Land Water ◽  
Biodiversity Information ◽  
Game Changer

AbstractDNA sampled from the environment (eDNA) is becoming a game changer for uncovering biodiversity patterns. By combining a conceptual model and empirical data, we test whether eDNA transported in river networks can be used as an integrative way to assess eukaryotic biodiversity for broad spatial scales and across the land-water interface. Using an eDNA metabarcode approach we detected 296 families of eukaryotes, spanning 19 phyla across the catchment of a river. We show for a subset of these families that eDNA samples overcome spatial autocorrelation biases associated with classical community assessments by integrating biodiversity information over space. Additionally, we demonstrate that many terrestrial species can be detected; thus revealing that eDNA in river-water also incorporates biodiversity information across terrestrial and aquatic biomes. Environmental DNA transported in river networks offers a novel and spatially integrated way to assess total biodiversity for whole landscapes and will transform biodiversity data acquisition in ecology.“Eventually, all things merge into one, 32 and a river runs through it.” — Norman Maclean

scholarly journals Detection and Integrated Analysis of LncRNA and mRNA Relevant to Plateau Adaptation of Yak

2019 ◽  
Author(s):  
Jiabo Wang ◽  
Zhixin Chai ◽  
Lei Deng ◽  
Jikun Wang ◽  
Hui Wang ◽  
...  
Keyword(s):  
Bos Taurus ◽  
Reference Method ◽  
R Package ◽  
Tibet Plateau ◽  
Integrated Analysis ◽  
Low Oxygen ◽  
Hub Genes ◽  
Protein Coding ◽  
Bos Grunniens ◽  
Ultraviolet Ray

Abstract Background: Yaks, which are known as “The ship in the Plateau”, play an important role in the life of people on the Qinghai-Tibet Plateau. There are many potential genetic advantages for Plateau Adaptation(PA) of Yak to improve breeding and adapt the environment with low oxygen and strong ultraviolet ray. In this study, in order to filter the Different Expression (DE) genes and new long non-coding RNAs (lncRNAs) ,also reveal underlying co-expression and regulation network of these, we sequenced 18 samples (cerebellum and cerebrum) of Bos Taurus, Bos Grunniens ☓ Bos Taurus and Bos Grunniens (3 individuals as replications for each species), ,and compared the relationship between modules and PA trait basing on the background of crossbreed genetics. Generalized linear model was used to analyze DE genes, and the different tissues as adjust variations in the model can reduce the noise of population structure. Results: On average, 50 million clean reads for each sample were obtained by the control of data quality. 12,072 pseudo lncRNAs were predicted in intersection of three software (CPC2, CNCI and CPAT). Total 4,257 significant DE transcripts were identified by using Ballgown R package (P<0.01) , 1021 of which were protein coding genes, 14were known lncRNAs, 661 were novel lncRNAs. By WGCNA, a co-expression network of DE mRNAs and lncRNAs with 6 modules was created to present functional relationship with PA trait. Our study provided a valuable sub-network composed by 8 hub genes (GRAMD3, MAST4, MICAL1, TLE1, ERAP1, PINK1, MRPL49 and IL34),.1of them was known lncRNA (LOC106700748) ,5 were novel lncRNAs (MSTRG247.1, MSTRG431.1, MSTRG422.1, MSTRG327.1 and MSTRG97.1) in the major module of the whole network. Conclusions: The functions of those hub genes are associated with blood pressure, reactive oxygen and metabolic process. The analysis of co-expressed genes with PA trait provides a reference way of the regulation mechanisms in PA of Yak and gets a reference method of the additive genetics expression detection between crossbreed and parents population as well.

scholarly journals Testing multiple substrates for terrestrial biodiversity monitoring using environmental DNA metabarcoding

2020 ◽  
Vol 20 (3) ◽  
pp. 732-745 ◽  
Author(s):  
Mieke Heyde ◽  
Michael Bunce ◽  
Grant Wardell‐Johnson ◽  
Kristen Fernandes ◽  
Nicole E. White ◽  
...  
Keyword(s):  
Environmental Dna ◽  
Biodiversity Monitoring ◽  
Multiple Substrates ◽  
Dna Metabarcoding ◽  
Terrestrial Biodiversity

scholarly journals Environmental DNA reveals that rivers are conveyer belts of biodiversity information

2016 ◽  
Vol 7 (1) ◽  
Author(s):  
Kristy Deiner ◽  
Emanuel A. Fronhofer ◽  
Elvira Mächler ◽  
Jean-Claude Walser ◽  
Florian Altermatt
Keyword(s):  
Environmental Dna ◽  
Biodiversity Information

scholarly journals Den characteristics and ecological significance of Marmota himalayana on the Qinghai-Tibet Plateau

2021 ◽  
Author(s):  
Shulin Wang ◽  
Fujiang Hou
Keyword(s):  
Warm Season ◽  
Weather Conditions ◽  
Tibet Plateau ◽  
Physical Parameters ◽  
Ecological Significance ◽  
Tunnel Length ◽  
Plant Characteristics ◽  
Bad Weather ◽  
Qinghai Tibet Plateau ◽  
Good Drainage

Dens are places for cavernicolous animals to hibernate, reproduce, and avoid predators and harsh weather conditions, and thus they have a vital impact on their survival. M. himalayana is the main large cavernicolous rodent on the Qinghai-Tibet Plateau. The analysis of den traits and their ecological functions can reveal mechanisms by which marmots have adapted to their environment, which is important for further understanding the ecological significance of this species. From July to August 2019 (warm season), we used unmanned aerial vehicles to fly at low altitudes and slow speeds to locate 131 marmot burrows (45 on shaded slopes, 51 on sunny slopes, and 35 on flat areas) in the northeastern Qinghai-Tibet Plateau. We then measured the physical characteristics (den density, entrance size, first tunnel length, volume, orientation and plant characteristics near the den entrance) of these dens on site. The physical parameters of the M. himalayana dens showed that they function to protect the marmots from natural enemies and bad weather, provide good drainage, and maintain a stable microclimate around the entrance. This is a result of the marmot’s adaptation to the harsh environment (cold and humidity) of the Qinghai-Tibet Plateau.

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