scholarly journals Dynamic consolidated bioprocessing for innovative lab-scale production of bacterial alkaline phosphatase from Bacillus paralicheniformis strain APSO

2021 ◽  
Vol 11 (1) ◽  
Author(s):  
Soad A. Abdelgalil ◽  
Nadia A. Soliman ◽  
Gaber A. Abo-Zaid ◽  
Yasser R. Abdel-Fattah
Keyword(s):  
Alkaline Phosphatase ◽  
Consolidated Bioprocessing ◽  
Black Liquor ◽  
Batch Cultivation ◽  
Efficient Production ◽  
Scale Production ◽  
Market Demand ◽  
Stirred Tank Bioreactor ◽  
Bacterial Alkaline Phosphatase ◽  
Bacillus Paralicheniformis

AbstractTo meet the present and forecasted market demand, bacterial alkaline phosphatase (ALP) production must be increased through innovative and efficient production strategies. Using sugarcane molasses and biogenic apatite as low-cost and easily available raw materials, this work demonstrates the scalability of ALP production from a newfound Bacillus paralicheniformis strain APSO isolated from a black liquor sample. Mathematical experimental designs including sequential Plackett–Burman followed by rotatable central composite designs were employed to select and optimize the concentrations of the statistically significant media components, which were determined to be molasses, (NH4)2NO3, and KCl. Batch cultivation in a 7-L stirred-tank bioreactor under uncontrolled pH conditions using the optimized medium resulted in a significant increase in both the volumetric and specific productivities of ALP; the alkaline phosphatase throughput 6650.9 U L−1, and µ = 0.0943 h−1; respectively, were obtained after 8 h that, ameliorated more than 20.96, 70.12 and 94 folds compared to basal media, PBD, and RCCD; respectively. However, neither the increased cell growth nor enhanced productivity of ALP was present under the pH-controlled batch cultivation. Overall, this work presents novel strategies for the statistical optimization and scaling up of bacterial ALP production using biogenic apatite.

scholarly journals Author Correction: Dynamic consolidated bioprocessing for innovative lab-scale production of bacterial alkaline phosphatase from Bacillus paralicheniformis strain APSO

2021 ◽  
Vol 11 (1) ◽  
Author(s):  
Soad A. Abdelgalil ◽  
Nadia A. Soliman ◽  
Gaber A. Abo-Zaid ◽  
Yasser R. Abdel-Fattah
Keyword(s):  
Alkaline Phosphatase ◽  
Consolidated Bioprocessing ◽  
Scale Production ◽  
Bacterial Alkaline Phosphatase ◽  
Bacillus Paralicheniformis

An amendment to this paper has been published and can be accessed via a link at the top of the paper.

scholarly journals Dissecting cellobiose metabolic pathway and its application in biorefinery through consolidated bioprocessing in Myceliophthora thermophila

2019 ◽  
Vol 6 (1) ◽  
Author(s):  
Jingen Li ◽  
Shuying Gu ◽  
Zhen Zhao ◽  
Bingchen Chen ◽  
Qian Liu ◽  
...  
Keyword(s):  
Malic Acid ◽  
Plant Biomass ◽  
Consolidated Bioprocessing ◽  
Cellulase Activity ◽  
Industrial Applications ◽  
Efficient Production ◽  
Myceliophthora Thermophila ◽  
Final Strain ◽  
Cellobiose Metabolism ◽  
Cost Efficient

Abstract Background Lignocellulosic biomass has long been recognized as a potential sustainable source for industrial applications. The costs associated with conversion of plant biomass to fermentable sugar represent a significant barrier to the production of cost-competitive biochemicals. Consolidated bioprocessing (CBP) is considered a potential breakthrough for achieving cost-efficient production of biomass-based fuels and commodity chemicals. During the degradation of cellulose, cellobiose (major end-product of cellulase activity) is catabolized by hydrolytic and phosphorolytic pathways in cellulolytic organisms. However, the details of the two intracellular cellobiose metabolism pathways in cellulolytic fungi remain to be uncovered. Results Using the engineered malic acid production fungal strain JG207, we demonstrated that the hydrolytic pathway by β-glucosidase and the phosphorolytic pathway by phosphorylase are both used for intracellular cellobiose metabolism in Myceliophthora thermophila, and the yield of malic acid can benefit from the energy advantages of phosphorolytic cleavage. There were obvious differences in regulation of the two cellobiose catabolic pathways depending on whether M. thermophila JG207 was grown on cellobiose or Avicel. Disruption of Mtcpp in strain JG207 led to decreased production of malic acid under cellobiose conditions, while expression levels of all three intracellular β-glucosidase genes were significantly up-regulated to rescue the impairment of the phosphorolytic pathway under Avicel conditions. When the flux of the hydrolytic pathway was reduced, we found that β-glucosidase encoded by bgl1 was the dominant enzyme in the hydrolytic pathway and deletion of bgl1 resulted in significant enhancement of protein secretion but reduction of malate production. Combining comprehensive manipulation of both cellobiose utilization pathways and enhancement of cellobiose uptake by overexpression of a cellobiose transporter, the final strain JG412Δbgl2Δbgl3 produced up to 101.2 g/L and 77.4 g/L malic acid from cellobiose and Avicel, respectively, which corresponded to respective yields of 1.35 g/g and 1.03 g/g, representing significant improvement over the starting strain JG207. Conclusions This is the first report of detailed investigation of intracellular cellobiose catabolism in cellulolytic fungus M. thermophila. These results provide insights that can be applied to industrial fungi for production of biofuels and biochemicals from cellobiose and cellulose.

Isolasi dan Potensi Enzim Hidrolase Bakteri Simbion Padina sp. dari Pantai Lengkuas Belitung

2021 ◽  
Vol 23 (1) ◽  
pp. 11-17
Author(s):  
Siti Nur Jannah ◽  
Yumna Rahmadias Hanifa ◽  
Adi Budi Utomo ◽  
Ashar Kurnia Dian Prambodo ◽  
Arina Tri Lunggani
Keyword(s):  
Marine Organism ◽  
Marine Ecosystem ◽  
Gram Negative Bacteria ◽  
Scale Production ◽  
Market Demand ◽  
Bacterial Isolates ◽  
Clear Zone ◽  
Plate Method ◽  
Sea Use ◽  
Industrial Scale Production

Marine organism is one of the riches in the ocean of Indonesia. The benefits of sea use for new products produced are widely used and have high market demand. Enzymes that have marine interests have unique properties and have good benefits for industry. This study aims to isolate the bacteria that have symbionts with Padina sp and determine the potential of the enzyme hydrolase produced by these bacteria. Isolation is done by the spread plate method. Pure isolates obtained were then tested for the potential of the enzyme hydrolase on selective media. Clear zone measurements are performed to determine which bacterial isolates are good for enzyme production. The results obtained by 6 isolates of pure bacteria, all of which include Gram negative bacteria that form bacilli. All isolates had the ability to produce different Protease, Lipase, Amylase and Cellulase enzymes. The enzymes obtained from these symbiotic bacteria are expected to be used for industrial-scale production in Indonesia. In addition, the presence of this symbiont bacteria is able to reduce the level of exploitation of Padina sp and contribute to preserving the marine ecosystem.

scholarly journals Trends in agriculture and food production

2009 ◽  
Vol 3 (1-2) ◽  
pp. 99-110
Author(s):  
János Lazányi
Keyword(s):  
Labour Productivity ◽  
Arable Land ◽  
Developed Countries ◽  
Strong Increase ◽  
Small Scale ◽  
Scale Production ◽  
Market Demand ◽  
Total Employment ◽  
Agricultural Reform ◽  
Agricultural Output

Agricultural reform resulted a shift from collective farming to small-scale production in China. This reform also has resulted a strong increase in gross agricultural output, which coincides with a slower increase in labour productivity. At the beginning of the reforms, agriculture accounted for 70 percent of total employment in China and still employs more than 50%. As a result of these reforms, China has undergone impressive economic growth also in the agriculture; the country has become one of the world’s top exporters and is attracting record amounts of foreign investment. The government has also stepped up investments in rural areas to meet the market demand for agricultural products. Results are very competitive compared to Central and Eastern European countries, where agriculture accounted for only 15 percent of total employment, but agricultural reform resulted a strong decline in gross agricultural output, which coincides with a similarly strong decline in employment. When approaching the issue of sustainable agriculture, we have to take into consideration, which China and India feed the largest populations in the world and both countries have had its own agricultural successes in the past 50 years. China has used land far more efficiently than many developed countries. With nine percent of the world’s arable land, China is responsible for the greatest share of agricultural production worldwide. Volume of produced pork, eggs, wheat, cotton, tobacco, and rice has increased and China exports an increasing amount of product each year. China has opened his borders, but do not expose food consumers to price shocks and producers to risks and disincentives. In this paper, the land-tenure system and the trends of agricultural developments are analysed in China and selected countries of EU.

scholarly journals Infection with intracellular parasite Amoeboaphelidium protococcarum induces shifts in associated bacterial communities in microalgae cultures

2021 ◽  
Vol 33 (5) ◽  
pp. 2863-2873
Author(s):  
Anna-Lena Höger ◽  
Carola Griehl ◽  
Matthias Noll
Keyword(s):  
Bacterial Communities ◽  
Large Scale ◽  
Algal Growth ◽  
Dry Weight ◽  
Illumina Miseq ◽  
Culture Conditions ◽  
Rrna Gene ◽  
Scale Production ◽  
Market Demand ◽  
Freshwater Microalgae

AbstractIn recent years microalgae products have developed increasing market demand, but sustainable industrial production is still challenged by biological stability of large-scale production plants. Yet the relationships between algal hosts, associated microbiomes, and contaminants in photobioreactors remains widely understudied. The aim of this study was to investigate the temporal development of microbiomes of four freshwater microalgae species Scenedesmus vacuolatus, Desmodesmus quadricauda, Chlorella sorokiniana, and Botryococcus braunii, in presence and absence of the zoosporic parasite Amoeboaphelidium protococcarum. To compare the effects of sterile and nonsterile culture conditions, infection experiments were performed in sterile laboratory (sterile) and simulated industrial conditions (open). Algal growth (dry weight, optical density, and nutrient consumption) was observed for 21 days, and samples of the associated microbiome were collected for bacterial 16S rRNA gene Illumina MiSeq sequencing. Infection patterns of A. protococcarum were algae species-specific, irrespectively of culture conditions. Bacterial community analysis demonstrated distinct and stable bacterial communities for each algae species, which were mostly dominated by α- and γ-Proteobacteria. Upon aphelid parasitosis, bacterial diversity increased, and community compositions diverged algae-specific over time. Moreover, bacterial functional traits shifted to detoxification, degradation, and cellulolysis once algae were infected. This study provides a first insight into the close connection between algae, associated bacterial microbiomes and appearing contaminants in photobioreactor systems.

A Test for Bacterial Alkaline Phosphatase: Use in Rapid Identification of Serratia Organisms

1973 ◽  
Vol 19 (11) ◽  
pp. 1248-1249 ◽  
Author(s):  
Paul L Wolf ◽  
Elisabeth Von der Muehll ◽  
Karen Praisler
Keyword(s):  
Alkaline Phosphatase ◽  
Clinical Laboratory ◽  
Rapid Identification ◽  
Hospitalized Patients ◽  
Coagulase Negative Staphylococcus ◽  
Blue Color ◽  
Bacterial Alkaline Phosphatase ◽  
Life Threatening ◽  
Life Threatening Complication ◽  
Coagulase Positive Staphylococcus

Abstract This investigation concerns identification of alkaline phosphatase production by bacterial organisms, as detected by a blue color resulting from conversion of indolyl phosphate to indigo. Coagulase-positive Staphylococcus produced alkaline phosphatase; coagulase-negative Staphylococcus did not. Serratia did not produce alkaline phosphatase; those Enterobacteriaceae we tested did. Thus, this test rapidly differentiates these organisms, diminishing the time for identification of Serratia in the clinical laboratory by 48 h. Identification of Serratia should not be ignored, because it is a life-threatening complication for certain hospitalized patients.

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