scholarly journals 10-hydroxy-2E-decenoic acid (10HDA) does not promote caste differentiation in Melipona scutellaris stingless bees

2021 ◽  
Vol 11 (1) ◽  
Author(s):  
Luiza Diniz Ferreira Borges ◽  
Letícia Leandro Batista ◽  
Serena Mares Malta ◽  
Tamiris Sabrina Rodrigues ◽  
Jéssica Regina da Costa Silva ◽  
...  
Keyword(s):  
Histone Deacetylases ◽  
Metabolic Regulation ◽  
Royal Jelly ◽  
Developmental Stages ◽  
Caste Differentiation ◽  
Decenoic Acid ◽  
Larval Food ◽  
Deacetylase Inhibitor ◽  
Genes Encoding ◽  
Environmental Component

AbstractIn bees from genus Melipona, differential feeding is not enough to fully explain female polyphenism. In these bees, there is a hypothesis that in addition to the environmental component (food), a genetic component is also involved in caste differentiation. This mechanism has not yet been fully elucidated and may involve epigenetic and metabolic regulation. Here, we verified that the genes encoding histone deacetylases HDAC1 and HDAC4 and histone acetyltransferase KAT2A were expressed at all stages of Melipona scutellaris, with fluctuations between developmental stages and castes. In larvae, the HDAC genes showed the same profile of Juvenile Hormone titers—previous reported—whereas the HAT gene exhibited the opposite profile. We also investigated the larvae and larval food metabolomes, but we did not identify the putative queen-fate inducing compounds, geraniol and 10-hydroxy-2E-decenoic acid (10HDA). Finally, we demonstrated that the histone deacetylase inhibitor 10HDA—the major lipid component of royal jelly and hence a putative regulator of honeybee caste differentiation—was unable to promote differentiation in queens in Melipona scutellaris. Our results suggest that epigenetic and hormonal regulations may act synergistically to drive caste differentiation in Melipona and that 10HDA is not a caste-differentiation factor in Melipona scutellaris.

10-hydroxy-2E-decenoic Acid (10HDA) is not a Caste-differentiation Factor in Melipona Scutellaris Stingless Bees

2020 ◽  
Author(s):  
Luiza Borges ◽  
Letícia Batista ◽  
Serena Malta ◽  
Tamiris Rodrigues ◽  
Jéssica Silva ◽  
...  
Keyword(s):  
Histone Deacetylases ◽  
Metabolic Regulation ◽  
Developmental Stages ◽  
Caste Differentiation ◽  
Endocrine Regulation ◽  
Decenoic Acid ◽  
Larval Food ◽  
Differentiation Factor ◽  
Genes Encoding ◽  
Environmental Component

Abstract In bees from genus Melipona, differential feeding is not enough to fully explain female polyphenism. In these bees, there is a hypothesis that in addition to the environmental component (food), a genetic component is also involved caste differentiation regulation. This mechanism has not yet been fully elucidated and may involve epigenetic and metabolic regulation. Here, we analysed the expression of the genes encoding histone deacetylases HDAC1 and HDAC4 and histone acetyltransferase KAT2A in Melipona scutellaris. We also investigated the metabolic profile of larvae and larval food to search for putative queen-fate inducing compounds. Finally, we assessed the effect of the histone deacetylase inhibitor 10-hydroxy-2E-decenoic acid (10HDA) - the major lipid component of royal jelly and hence a putative regulator of honeybee caste differentiation - on Melipona caste differentiation. The hdac1, hdac4 and kat2a transcripts were expressed at all stages, with fluctuations in developmental stages and castes, which may be related to endocrine regulation. We did not identify the putative caste-differentiation factors, geraniol and 10HDA. Also, 10HDA was unable to promote differentiation in queens. Our results suggest that epigenetic and hormonal regulations act synergistically for drive caste differentiation in Melipona and that 10HDA is not a caste-differentiation factor in Melipona scutellaris.

LARVAL REARING BY WORKER HONEY BEES LACKING THEIR MANDIBULAR GLANDS: II. REARING BY LARGER NUMBERS OF WORKER BEES

1979 ◽  
Vol 111 (1) ◽  
pp. 101-104 ◽  
Author(s):  
Ying-Shin Peng ◽  
S. C. Jay
Keyword(s):  
Honey Bees ◽  
Royal Jelly ◽  
Mandibular Gland ◽  
Caste Differentiation ◽  
Larval Rearing ◽  
Mandibular Glands ◽  
Larval Food ◽  
Mandibular Gland Secretion ◽  
Final Development ◽  
Queen Cell

AbstractFurther experiments were done in an attempt to ascertain the significance of the mandibular glands of nurse bees in female caste differentiation. Groups of 200, 10-day-old nurse bees, with their mandibular glands removed, fed female larvae for 80 h in plastic queen cell cups in the laboratory. After this, each larva finished feeding in a 4-day-old queen cell containing "royal jelly"; final development occurred in an artificial pupation dish. Because four adults, classified as "queenlike intermediates," were reared it appears that (1) mandibular gland secretion is less important as a larval food than that of the hypopharyngeal glands, and (2) if a "queen determining substance" exists the mandibular glands are not its only source.

Quantitative Untersuchungen über das Vorkommen von Vitamin B6 bei den drei Kasten der Honigbiene, Apis mellifera / Explorations on Quantity of Vitamine B6 in the three Castes of Honey-Bee Apis mellifera

1971 ◽  
Vol 26 (9) ◽  
pp. 956-961 ◽  
Author(s):  
Gisela Hanser
Keyword(s):  
Apis Mellifera ◽  
Larval Development ◽  
Honey Bee ◽  
Vitamin B6 ◽  
Royal Jelly ◽  
Developmental Stages ◽  
Vitamin B ◽  
Prepupal Stage ◽  
Larval Food ◽  
Dependent Change

Royal jelly contains a three fold higher concentration of vitamin B6 than the larval food for workers or drones (♀ = 20,59 μg,♀= 7,34 μg, ♂ = 7,23 μg pro g freshweight). Similar differences in the B6-concentrations are also observed in the three bee castes at corresponding developmental stages. The relatively high vitamin B6 concentration in the young larvae of all three castes decreases in the course of larval development before the prepupal stage. A seasonal dependent change in the Be values is shown in the worker jelly by the increase from 3,78 μg/g freshweight in May to 8,45 μg/g freshweight in July/August. The similarity in the vitamin B6 content in the larval food and in the bees of same age indicates the identity of the drone and worker jellies. The hypopharyngeal and post-cerebral glands of queen rearing nurse bees contain more vitamin B6 than the corresponding glands of normal nurse bees of the same age.

Genetic regulation of nitrogen metabolism in the fungi

1997 ◽  
Vol 61 (1) ◽  
pp. 17-32
Author(s):  
G A Marzluf
Keyword(s):  
Nitrogen Metabolism ◽  
Protein Interactions ◽  
Fungal Pathogens ◽  
Metabolic Regulation ◽  
Specific Binding ◽  
Genetic Regulation ◽  
Nitrogen Sources ◽  
Specific Protein ◽  
Genes Encoding ◽  
Gata Family

In the fungi, nitrogen metabolism is controlled by a complex genetic regulatory circuit which ensures the preferential use of primary nitrogen sources and also confers the ability to use many different secondary nitrogen sources when appropriate. Most structural genes encoding nitrogen catabolic enzymes are subject to nitrogen catabolite repression, mediated by positive-acting transcription factors of the GATA family of proteins. However, certain GATA family members, such as the yeast DAL80 factor, act negatively to repress gene expression. Selective expression of the genes which encode enzymes for the metabolism of secondary nitrogen sources is often achieved by induction, mediated by pathway-specific factors, many of which have a GAL4-like C6/Zn2 DNA binding domain. Regulation within the nitrogen circuit also involves specific protein-protein interactions, as exemplified by the specific binding of the negative-acting NMR protein with the positive-acting NIT2 protein of Neurospora crassa. Nitrogen metabolic regulation appears to play a significant role in the pathogenicity of certain animal and plant fungal pathogens.

scholarly journals Royal Jelly Aliphatic Acids Contribute to Antimicrobial Activity of Honey

2018 ◽  
Vol 62 (1) ◽  
pp. 111-123 ◽  
Author(s):  
Walerij Isidorow ◽  
Stanisław Witkowski ◽  
Piotr Iwaniuk ◽  
Monika Zambrzycka ◽  
Izabela Swiecicka
Keyword(s):  
Fatty Acids ◽  
Antimicrobial Activity ◽  
Antibacterial Activity ◽  
Royal Jelly ◽  
Dicarboxylic Acids ◽  
Antibacterial Action ◽  
Larval Food ◽  
Nutrient Media ◽  
Minimal Inhibitory Concentrations ◽  
Aliphatic Acids

Abstract Honey is valued for its therapeutic qualities which are attributed among others to its antibacterial multifactorial properties. However, all the factors that influence these properties have not been identified. The present study is focused on the antibacterial action of fatty acids originating from royal jelly, the larval food of honeybees. Aliphatic C8-C12 acids characteristic of this bee product had previously been identified in more than fifty different samples of honey originating from seven countries and in eleven samples of Polish herbhoney. Experiments were performed to ascertain the influence of acidity on the antimicrobial activity of the acids. In acidic nutrient media all tested aliphatic hydroxyacids and unsaturated dicarboxylic acids demonstrated antibacterial action against different microbes with minimal inhibitory concentrations between 0.048 and 3.125 mM. Our results confirm that part of the antibacterial activity of honey contributes to these compounds of bee origin.

Sign in / Sign up

Export Citation Format

Share Document