scholarly journals Novel moisturized and antimicrobial hand gel based on zinc-aminoclay and Opuntia humifusa extract

2021 ◽  
Vol 11 (1) ◽  
Author(s):  
Hien Thi Hoang ◽  
Vinh Van Tran ◽  
Vu Khac Hoang Bui ◽  
Oh-Hyeok Kwon ◽  
Ju-Young Moon ◽  
...  
Keyword(s):  
Antimicrobial Activity ◽  
Skin Irritation ◽  
Gram Negative Bacteria ◽  
Skin Protection ◽  
Toxicity Evaluation ◽  
High Antimicrobial Activity ◽  
Gram Positive Bacteria ◽  
Opuntia Humifusa ◽  
Standard Level ◽  
Low Toxicity

AbstractThe high antimicrobial ability and low toxicity of zinc-aminoclay (ZnAC) are claimed in our previous reports. In this study, we formulate a novel hand gel based on ZnAC and Opuntia humifusa (O. humifusa) extract, which is a high moisturizing agent. The antimicrobial activity, cytotoxicity, moisturizing effect, and clinical skin irritation of the hand gel are evaluated. The hand gel with 0.5 wt.% ZnAC and 1.0 v/v% O. humifusa extract can kill more than 99% Escherichia coli (gram-negative bacteria) and Staphylococcus aureus (gram-positive bacteria) after 24 h. Toxicity evaluation shows that, the hand gel does not affect the viability of mammalian HaCaT cells. Additionally, skin moisture is increased by applying the hand gel while its viscosity is at the standard level of commercial products. The hand gel has a skin irritation index of 0.0 and is classified as a non-irritating product. We successfully formulated hand gel from ZnAC, glucomannan, glycerol, and O. humifusa extract. Owing to the high antimicrobial activity and skin protection of hand gels, they are suitable to be used as hand sanitizers in restaurants, hospitals, and homes effectively.

scholarly journals Composition and Antimicrobial Analysis of the Essential Oil of Litsea Laevigata Nees. (Lauraceae)

2008 ◽  
Vol 3 (7) ◽  
pp. 1934578X0800300 ◽  
Author(s):  
Arif M Muhammed ◽  
Raj M Subbu ◽  
Leopold Jirovetz ◽  
Shafi P Mohamed
Keyword(s):  
Antimicrobial Activity ◽  
Aspergillus Niger ◽  
Essential Oil ◽  
Western Ghats ◽  
Klebsiella Aerogenes ◽  
Gram Negative Bacteria ◽  
Proteus Vulgaris ◽  
High Antimicrobial Activity ◽  
Gram Positive ◽  
Gram Positive Bacteria

The essential oil of the berries of Litsea laevigata Nees., growing wild in Western Ghats, Kerala, India, was obtained by hydrodistillation and was fractionated by passage through a column using n-pentane and diethyl ether as eluents. The essential oil and its fractions were analysed by GC and GC-MS. Twenty seven compounds representing 99.2% of the original oil were identified. The major components are the terpene hydrocarbons trans-α-bergamotene(26.7%), α-pinene (25%) and β-pinene (8.2%). The antimicrobial activity of the essential oil and its fractions against four Gram-positive and four Gram-negative bacteria ( Staphylococcus aureus, Bacillus subtilis, Streptococcus faecalis, Staphylococcus albus, Escherichia coli, Pseudomonas aeruginosa, Proteus vulgaris, Klebsiella aerogenes), as well as two fungi ( Candida albicans, Aspergillus niger) was studied. The bioassay showed that the oil exhibited moderate to high antimicrobial activity, being very active against gram-positive bacteria, such as Streptococcus albus, and the fungus Aspergillus niger.

Green Synthesis of Silver Nanoparticle Using Banana (Musa) Sap

2015 ◽  
Vol 1086 ◽  
pp. 7-10 ◽  
Author(s):  
S. Deena ◽  
Arun Dakshinamurthy ◽  
Paulraj Mosae Selvakumar
Keyword(s):  
Antimicrobial Activity ◽  
Silver Nanoparticles ◽  
Green Synthesis ◽  
Silver Nanoparticle ◽  
Gram Negative Bacteria ◽  
Visible Spectroscopy ◽  
High Antimicrobial Activity ◽  
Gram Positive Bacteria ◽  
Coated Fabric ◽  
Uv Visible

Green synthesis of silver nanoparticles using banana sap as a solvent, reducing agent and capping agent is reported in this work. Banana sap has also been used as a binder to incorporate silver nanoparticles into cotton fabric. UV-Visible spectroscopy was used to monitor the quantitative formation of silver nanoparticles. Silver nanoparticle coated fabric showed high antimicrobial activity against gram negative bacteria such asPseudomonas aureginosaandEscherichia coli, and gram positive bacteria such asBacillus subtilisandStaphylococcus aureus.KeywordsGreen synthesis, Silver nanoparticles, banana sap, antimicrobial activity

Disinfection of Fruits with Activated Water

2019 ◽  
Vol 10 ◽  
pp. 1864-1872
Author(s):  
Prof. Teodora P. Popova
Keyword(s):  
Antimicrobial Activity ◽  
Aqueous Solutions ◽  
Antimicrobial Properties ◽  
The Other ◽  
Gram Negative Bacteria ◽  
High Antimicrobial Activity ◽  
Gram Negative ◽  
E Coli ◽  
Number Of Microorganisms ◽  
Lower Effect

The effect of ionized aqueous solutions (anolytes and catholyte) in the processing of fruits (cherries, morellos, and strawberries) for decontamination has been tested. Freshly prepared analytes and catholyte without the addition of salts were used, as well as stored for 7 months anolytes, prepared with 0.5% NaCl and a combination of 0.5% NaCl and 0.5% Na2CO3. The anolyte prepared with a combination of 0.5% NaCl and 0.5% Na2CO3, as well as the anolyte obtained with 0.5% NaCl, exhibit high antimicrobial activity against the surface microflora of strawberries, cherries, and sour cherries. They inactivate E. coli for 15 minutes. The other species of the fam. Enterobacteriaceae were also affected to the maximum extent, as is the total number of microorganisms, especially in cherries and sour cherries. Even stored for 7 months, they largely retain their antimicrobial properties. Anolyte and catholyte, obtained without the addition of salts, showed a lower effect on the total number of microorganisms, but had a significant effect on Gram-negative bacteria, and especially with regard to the sanitary indicative E. coli.

scholarly journals Enaminone-Derived Pyrazoles with Antimicrobial Activity

2019 ◽  
Vol 2019 ◽  
pp. 1-10
Author(s):  
Mashooq Ahmad Bhat ◽  
Mohamed A. Al-Omar ◽  
Ahmed M. Naglah ◽  
Abdul Arif Khan
Keyword(s):  
Antimicrobial Activity ◽  
Gram Negative Bacteria ◽  
Significant Activity ◽  
Disc Diffusion ◽  
Initial Screening ◽  
Gram Positive ◽  
Gram Negative ◽  
Disc Diffusion Assay ◽  
Gram Positive Bacteria ◽  
Diffusion Assay

A series of pyrazoles derived from the substituted enaminones were synthesized and were evaluated for antimicrobial activity. All the compounds were characterized by the spectral data and elemental analysis. The synthesized compounds were initially screened for their antimicrobial activity against ATCC 6538, NCTC 10400, NCTC 10418, and ATCC 27853. During initial screening, compounds (P1, P6, and P11) presented significant antimicrobial activity through disc diffusion assay. These compounds were further evaluated for antimicrobial activity at different time points against Gram-positive and Gram-negative bacteria and presented significant activity for 6 hours. The activity was found to be greater against Gram-positive bacteria. In contrast at 24 hours, the activity was found only against Gram-positive bacteria except compound (P11), showing activity against both types of bacteria. Compound (P11) was found to have highest activity against both Gram-positive and Gram-negative bacteria.

Antimicrobial activity of Pycnarrhena cauliflora (Miers.) Diels. methanol extract

2021 ◽  
Vol 2 (2) ◽  
Author(s):  
Eti Nurwening Sholikhah ◽  
Maulina Diah ◽  
Mustofa ◽  
Masriani ◽  
Susi Iravati ◽  
...  
Keyword(s):  
Antimicrobial Activity ◽  
Methanolic Extract ◽  
Biological Activities ◽  
Positive Control ◽  
Diffusion Method ◽  
Gram Negative Bacteria ◽  
Gram Positive ◽  
Gram Negative ◽  
Gram Positive Bacteria ◽  
Methanolic Extracts

Pycnarrhena cauliflora (Miers.) Diels., local name sengkubak, is one of indigenous plants from West Kalimantan that has been used as natural flavor. Pycnorrhena cauliflora is one of species of Menispermaceae family which is rich in bisbenzylisoquinoline alkaloids. This alkaloids are known to have various biological activities including antiprotozoal, antiplasmodial, antifungal and antibacterial activities. This study aimed to investigate antimicrobial activity of  the P. cauliflora (Miers.) Diels. methanolic extracts against gram-positive and gram-negative bacteria. The methanolic extract of P. cauliflora (Miers.) Diels., root, leaf and stem were prepared by maceration. The disk-diffusion method was then used to determine the antimicrobial activity of the extracts against Streptococcus pyogenes, S. mutants, Staphylococcus aureus, S. epidermidis, Salmonella typhi, Shigella flexneri, Pseudomonas aeruginosa and Escherichia coli after 18-24 h incubation at 37 oC. Amoxicillin was used as positive control for gram-positive bacteria and ciprofloxacin was used as gram-negative bacteria. The inhibition zones were then measured in mm. Analysis were conducted in duplicates. The results showed in general the methanolic extracts of P. cauliflora (Miers.) Diels. root (inhibition zone diameter= 10-23 mm) were more active than that leaf (0-15 mm) and stem (0-17 mm) extracts against gram-positive bacteria. The zone inhibition diameter of amoxicillin as positive control was 8-42 mm. In addition, the methanolic extracts of P. cauliflora (Miers.) Diels. root (12-17 mm) were also more active than that leaf (0-12 mm) and stem (0-12 mm) extracts against gram-negative bacteria. The zone inhibition diameter of ciprofloxacin as positive control was 33-36 mm. In conclusion, the methanolic extract of P. caulifloria (Miers.) Diels. root is the most extract active against both gram-positive and gram-negative bacteria. Further study will be focused to isolate active compounds in the methanolic extract of the root.

Optimization of Silver Nanoparticles Production using Aspergillus niger and Study of Antimicrobial Activity

2021 ◽  
Vol 12 (4) ◽  
pp. 2383-2388
Author(s):  
Suguna Selvakumaran ◽  
Kayathri Marimuthu ◽  
Thiruvany Poopalan ◽  
Kalaiyarasi Tamil Selvan ◽  
Nozieana Khairuddin
Keyword(s):  
Antimicrobial Activity ◽  
Silver Nanoparticles ◽  
Aspergillus Niger ◽  
Culture Supernatant ◽  
Gram Negative Bacteria ◽  
Mixing Ratio ◽  
Zone Of Inhibition ◽  
Gram Positive Bacteria ◽  
Physiochemical Parameters ◽  
Uv Visible

Silver nanoparticles have attracted high attention worldwide for their various applications. The physiochemical parameters such as temperature, media, mixing ratio affect the rate of synthesis of silver nanoparticles and their yield. Thus, optimization of these physiochemical parameters is needed to enhance the production of silver nanoparticles. In this study, silver nanoparticles were synthesized using Aspergillus niger culture supernatant. The produced silver nanoparticles were characterized using UV-visible Spectrophotometer at 200 nm to 700 nm, which had a peak at 450 nm, indicates the formation of silver nanoparticles. It was found that Sabouraud Dextrose Broth (SDB) as optimum media, 40 ml of supernatant and 10 ml of silver nitrate as optimum mixing ratio and 65°C as optimum temperature to produce silver nanoparticles. The optimized silver nanoparticles were subjected to antimicrobial activity, and it was found that it is highly effective towards gram-negative bacteria than gram-positive bacteria where the zone of inhibition for Escherichia coli was  7 ± 2.7 mm and 5.3 ± 2.1 mm for Staphylococcus aureus.

ANTIMICROBIAL ACTIVITY OF COPPER KAOLINITE AND SURFACTANT MODIFIED COPPER KAOLINITE AGAINST GRAM POSITIVE AND GRAM NEGATIVE BACTERIA

2016 ◽  
Vol 78 (3-2) ◽  
Author(s):  
Nor Syafawani Sarah Md Saad ◽  
Nik Ahmad Nizam Nik Malek ◽  
Chun Shiong Chong
Keyword(s):  
Antimicrobial Activity ◽  
Synergistic Effects ◽  
Wide Spectrum ◽  
Copper Nitrate ◽  
Gram Negative Bacteria ◽  
Gram Positive ◽  
Gram Negative ◽  
X Ray ◽  
Gram Positive Bacteria ◽  
Diffusion Technique

The aim of this research was to determine the antimicrobial activity of kaolinite modified with antimicrobial compounds against Gram positive and Gram negative bacteria. Copper kaolinite (Cu-kaolinite) was prepared by loading raw kaolinite with copper nitrate trihydrate (CuNO3) while surfactant modified Cu-kaolinite (SM-Cu-kaolinite) was prepared by adding cationic surfactants hexadecyltrimethyl ammonium (HDTMA) on Cu-Kaolinite. Samples was characterized by Fourier transform infrared spectroscopy (FTIR), X-ray diffraction (XRD), scanning electron microscopy (SEM) and energy dispersive X-ray (EDX) analyzer. The antimicrobial activity of the samples was tested against Gram negative bacteria (Escherichia coli ATCC 11229 and Pseudomonas aeruginosa ATCC 15442), and Gram positive bacteria (Staphylococcus aureus ATCC 6538 and Enterococcus faecalis ATCC 29212) through disc diffusion technique (DDT) and minimum inhibition concentration (MIC). The results showed that the antimicrobial activity of Cu-kaolinite increased after modified with HDTMA due to the synergistic effects of Cu ions and HDTMA molecules on the kaolinite. The antimicrobial activity for surfactant modified Cu-kaolinite was greater for Gram positive bacteria compared to Gram negative bacteria. In conclusion, the attachment of HDTMA on Cu-kaolinite contributed to the enhanced antimicrobial activity against wide spectrum of bacteria (Gram positive and Gram negative bacteria).

scholarly journals Increases in Hydrophilicity and Charge on the Polar Face of Alyteserin 1c Helix Change its Selectivity towards Gram-Positive Bacteria

Antibiotics ◽  
2019 ◽  
Vol 8 (4) ◽  
pp. 238 ◽  
Author(s):  
Yamil Liscano ◽  
Constain H. Salamanca ◽  
Lina Vargas ◽  
Stefania Cantor ◽  
Valentina Laverde-Rojas ◽  
...  
Keyword(s):  
Antimicrobial Activity ◽  
Solid Phase ◽  
Structural Characteristics ◽  
Gram Negative Bacteria ◽  
Gram Positive ◽  
Gram Negative ◽  
Gram Positive Bacteria ◽  
Mutant Derivative ◽  
Conventional Antibiotics ◽  
Carboxy Terminal

Recently, resistance of pathogens towards conventional antibiotics has increased, representing a threat to public health globally. As part of the fight against this, studies on alternative antibiotics such as antimicrobial peptides have been performed, and it has been shown that their sequence and structure are closely related to their antimicrobial activity. Against this background, we here evaluated the antibacterial activity of two peptides developed by solid-phase synthesis, Alyteserin 1c (WT) and its mutant derivative (ΔM), which shows increased net charge and reduced hydrophobicity. These structural characteristics were modified as a result of amino acid substitutions on the polar face of the WT helix. The minimum inhibitory concentration (MIC) of both peptides was obtained in Gram-positive and Gram-negative bacteria. The results showed that the rational substitutions of the amino acids increased the activity in Gram-positive bacteria, especially against Staphylococcus aureus, for which the MIC was one-third of that for the WT analog. In contrast to the case for Gram-positive bacteria, these substitutions decreased activity against Gram-negative bacteria, especially in Escherichia coli, for which the MIC was eight-fold higher than that exhibited by the WT peptide. To understand this, models of the peptide behavior upon interacting with membranes of E. coli and S. aureus created using molecular dynamics were studied and it was determined that the helical stability of the peptide is indispensable for antimicrobial activity. The hydrogen bonds between the His20 of the peptides and the phospholipids of the membranes should modulate the selectivity associated with structural stability at the carboxy-terminal region of the peptides.

scholarly journals Synthesis and Antimicrobial Activity of a Silver-Hydroxyapatite Nanocomposite

2009 ◽  
Vol 2009 ◽  
pp. 1-6 ◽  
Author(s):  
Marcos Díaz ◽  
Flora Barba ◽  
Miriam Miranda ◽  
Francisco Guitián ◽  
Ramón Torrecillas ◽  
...  
Keyword(s):  
Antimicrobial Activity ◽  
Reduction Process ◽  
Bactericidal Effect ◽  
Gram Negative Bacteria ◽  
Subsequent Reduction ◽  
Visible Spectroscopy ◽  
High Antimicrobial Activity ◽  
Chemical Route ◽  
Uv Visible ◽  
High Degree

A silver-hydroxyapatite nanocomposite has been obtained by a colloidal chemical route and subsequent reduction process in H2/Ar atmosphere at350∘C. This material has been characterized by TEM, XRD, and UV-Visible spectroscopy, showing the silver nanoparticles (∼65 nm) supported onto the HA particles (∼130 nm) surface without a high degree of agglomeration. The bactericidal effect against common Gram-positive and Gram-negative bacteria has been also investigated. The results indicated a high antimicrobial activity forStaphylococcus aureus, PneumococcusandEscherichia coli,so this material can be a promising antimicrobial biomaterial for implant and reconstructive surgery applications.

Antimicrobial Activity of Fractions and Compounds from Calophyllum brasiliense (Clusiaceae/Guttiferae)

2004 ◽  
Vol 59 (9-10) ◽  
pp. 657-662 ◽  
Author(s):  
Juliana B. Pretto ◽  
Valdir Cechinel-Filho ◽  
Vânia F. Noldin ◽  
Mara R. K. Sartori ◽  
Daniela E. B Isaias ◽  
...  
Keyword(s):  
Antimicrobial Activity ◽  
Pathogenic Bacteria ◽  
Protocatechuic Acid ◽  
Inhibitory Concentration ◽  
Gram Negative Bacteria ◽  
Gram Positive ◽  
Gram Positive Bacteria ◽  
Methanolic Extracts ◽  
Calophyllum Brasiliense

Abstract Calophyllum brasiliense (Clusiaceae/Guttiferae) is a native Brazilian medicinal plant traditionally used against several diseases, including infectious pathologies. Crude methanolic extracts (CME) and two fractions, denoted non-polar (soluble in chloroform) and polar (nonsoluble in chloroform), were prepared from different parts of the plant (roots, stems, leaves, flowers and fruits) and studied. The following compounds were isolated and tested against pathogenic bacteria and yeasts by determination of the minimal inhibitory concentration (MIC): brasiliensic acid (1), gallic acid (2), epicatechin (3), protocatechuic acid (4), friedelin (5) and 1,5-dihydroxyxanthone (6). The results indicated that all the parts of the plant exhibited antimicrobial activity against Gram-positive bacteria, which are selectively inhibited by components of C. brasiliense. No activity was observed against Gram-negative bacteria and yeasts tested. Regarding the isolated compounds, substance 4 showed antimicrobial activity against all the tested microorganisms, whereas compound 6 exhibited antimicrobial activity only against Gram-positive bacteria. The results from the current study confirm and justify the popular use of this plant to treat infectious processes.

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