Identification and characterization of piperine synthase from black pepper, Piper nigrum L.

AbstractBlack pepper (Piper nigrum L.) is the world’s most popular spice and is also used as an ingredient in traditional medicine. Its pungent perception is due to the interaction of its major compound, piperine (1-piperoyl-piperidine) with the human TRPV-1 or vanilloid receptor. We now identify the hitherto concealed enzymatic formation of piperine from piperoyl coenzyme A and piperidine based on a differential RNA-Seq approach from developing black pepper fruits. This enzyme is described as piperine synthase (piperoyl-CoA:piperidine piperoyl transferase) and is a member of the BAHD-type of acyltransferases encoded by a gene that is preferentially expressed in immature fruits. A second BAHD-type enzyme, also highly expressed in immature black pepper fruits, has a rather promiscuous substrate specificity, combining diverse CoA-esters with aliphatic and aromatic amines with similar efficiencies, and was termed piperamide synthase. Recombinant piperine and piperamide synthases are members of a small gene family in black pepper. They can be used to facilitate the microbial production of a broad range of medicinally relevant aliphatic and aromatic piperamides based on a wide array of CoA-donors and amine-derived acceptors, offering widespread applications.

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Genome wide identification and characterization of microsatellite markers in black pepper (Piper nigrum): A valuable resource for boosting genomics applications

PLoS ONE ◽

10.1371/journal.pone.0226002 ◽

2019 ◽

Vol 14 (12) ◽

pp. e0226002 ◽

Cited By ~ 1

Author(s):

Ratna Kumari ◽

Dhammaprakash Pandhari Wankhede ◽

Akansha Bajpai ◽

Avantika Maurya ◽

Kartikay Prasad ◽

...

Keyword(s):

Microsatellite Markers ◽

Black Pepper ◽

Piper Nigrum ◽

Valuable Resource ◽

Genome Wide ◽

Identification And Characterization

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Genome-Wide Analysis, Evolutionary History and Response of ALMT Family to Phosphate Starvation in Brassica napus

International Journal of Molecular Sciences ◽

10.3390/ijms22094625 ◽

2021 ◽

Vol 22 (9) ◽

pp. 4625

Author(s):

Ismail Din ◽

Ihteram Ullah ◽

Wei Wang ◽

Hao Zhang ◽

Lei Shi

Keyword(s):

Brassica Napus ◽

Gene Family ◽

P Availability ◽

Rna Seq ◽

Soil P ◽

Genome Wide ◽

Low Phosphorus ◽

Leaf Tissues ◽

Identification And Characterization

Low phosphorus (P) availability is one of the major constraints to plant growth, particularly in acidic soils. A possible mechanism for enhancing the use of sparsely soluble P forms is the secretion of malate in plants by the aluminum-activated malate transporter (ALMT) gene family. Despite its significance in plant biology, the identification of the ALMT gene family in oilseed rape (Brassica napus; B. napus), an allotetraploid crop, is unveiled. Herein, we performed genome-wide identification and characterization of ALMTs in B. napus, determined their gene expression in different tissues and monitored transcriptional regulation of BnaALMTs in the roots and leaves at both a sufficient and a deficient P supply. Thirty-nine BnaALMT genes were identified and were clustered into five branches in the phylogenetic tree based on protein sequences. Collinearity analysis revealed that most of the BnaALMT genes shared syntenic relationships among BnaALMT members in B. napus, which suggested that whole-genome duplication (polyploidy) played a major driving force for BnaALMTs evolution in addition to segmental duplication. RNA-seq analyses showed that most BnaALMT genes were preferentially expressed in root and leaf tissues. Among them, the expression of BnaC08g13520D, BnaC08g15170D, BnaC08g15180D, BnaC08g13490D, BnaC08g13500D, BnaA08g26960D, BnaC05g14120D, BnaA06g12560D, BnaC05g20630D, BnaA07g02630D, BnaA04g15700D were significantly up-regulated in B. napus roots and leaf at a P deficient supply. The current study analyzes the evolution and the expression of the ALMT family in B. napus, which will help in further research on their role in the enhancement of soil P availability by secretion of organic acids.

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Identification and characterization of stress degradation products of piperine and profiling of a black pepper (Piper nigrum L.) extract using LC/Q-TOF-dual ESI-MS

Analytical Methods ◽

10.1039/c4ay01088d ◽

2014 ◽

Vol 6 (19) ◽

pp. 8022-8029 ◽

Cited By ~ 13

Author(s):

Subhash Chandra Bose. Kotte ◽

P. K. Dubey ◽

P. M. Murali

Keyword(s):

Degradation Products ◽

Black Pepper ◽

Piper Nigrum ◽

Ionization Mass ◽

Spectrometry Method ◽

Hydrolysis Products ◽

Stress Degradation ◽

Identification And Characterization ◽

Dual Electrospray Ionization

A rapid, specific and reliable HPLC combined with quadrupole time-of-flight dual electrospray ionization mass spectrometry method has been developed and validated for the identification and characterization of stressed degradation (oxidation and hydrolysis) products of piperine.

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Transcriptome-wide identification and characterization of the MYB gene family in sickle seagrass (Thalassia hemprichii)

Ecological Genetics and Genomics ◽

10.1016/j.egg.2021.100093 ◽

2021 ◽

pp. 100093

Author(s):

Shen Jie ◽

Cai Zefu ◽

Chen Shiquan ◽

Wang Daoru ◽

Wu Zhongjie

Keyword(s):

Gene Family ◽

Thalassia Hemprichii ◽

Myb Gene ◽

Identification And Characterization

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Genome-Wide Identification and Characterization of Hsf and Hsp Gene Families and Gene Expression Analysis under Heat Stress in Eggplant (Solanum melongema L.)

Horticulturae ◽

10.3390/horticulturae7060149 ◽

2021 ◽

Vol 7 (6) ◽

pp. 149

Author(s):

Chao Gong ◽

Qiangqiang Pang ◽

Zhiliang Li ◽

Zhenxing Li ◽

Riyuan Chen ◽

...

Keyword(s):

Heat Stress ◽

Gene Families ◽

High Temperature Stress ◽

Pcr Analysis ◽

Rna Seq ◽

Genome Wide ◽

Motif Composition ◽

Hsp Genes ◽

Identification And Characterization

Under high temperature stress, a large number of proteins in plant cells will be denatured and inactivated. Meanwhile Hsfs and Hsps will be quickly induced to remove denatured proteins, so as to avoid programmed cell death, thus enhancing the thermotolerance of plants. Here, a comprehensive identification and analysis of the Hsf and Hsp gene families in eggplant under heat stress was performed. A total of 24 Hsf-like genes and 117 Hsp-like genes were identified from the eggplant genome using the interolog from Arabidopsis. The gene structure and motif composition of Hsf and Hsp genes were relatively conserved in each subfamily in eggplant. RNA-seq data and qRT-PCR analysis showed that the expressions of most eggplant Hsf and Hsp genes were increased upon exposure to heat stress, especially in thermotolerant line. The comprehensive analysis indicated that different sets of SmHsps genes were involved downstream of particular SmHsfs genes. These results provided a basis for revealing the roles of SmHsps and SmHsp for thermotolerance in eggplant, which may potentially be useful for understanding the thermotolerance mechanism involving SmHsps and SmHsp in eggplant.

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