scholarly journals Genome wide identification and characterization of microsatellite markers in black pepper (Piper nigrum): A valuable resource for boosting genomics applications

PLoS ONE ◽  
2019 ◽  
Vol 14 (12) ◽  
pp. e0226002 ◽  
Author(s):  
Ratna Kumari ◽  
Dhammaprakash Pandhari Wankhede ◽  
Akansha Bajpai ◽  
Avantika Maurya ◽  
Kartikay Prasad ◽  
...  
Keyword(s):  
Microsatellite Markers ◽  
Black Pepper ◽  
Piper Nigrum ◽  
Valuable Resource ◽  
Genome Wide ◽  
Identification And Characterization

Identification and characterization of stress degradation products of piperine and profiling of a black pepper (Piper nigrum L.) extract using LC/Q-TOF-dual ESI-MS

2014 ◽  
Vol 6 (19) ◽  
pp. 8022-8029 ◽  
Author(s):  
Subhash Chandra Bose. Kotte ◽  
P. K. Dubey ◽  
P. M. Murali
Keyword(s):  
Degradation Products ◽  
Black Pepper ◽  
Piper Nigrum ◽  
Ionization Mass ◽  
Spectrometry Method ◽  
Hydrolysis Products ◽  
Stress Degradation ◽  
Identification And Characterization ◽  
Dual Electrospray Ionization

A rapid, specific and reliable HPLC combined with quadrupole time-of-flight dual electrospray ionization mass spectrometry method has been developed and validated for the identification and characterization of stressed degradation (oxidation and hydrolysis) products of piperine.

scholarly journals Identification and characterization of piperine synthase from black pepper, Piper nigrum L.

2021 ◽  
Vol 4 (1) ◽  
Author(s):  
Arianne Schnabel ◽  
Benedikt Athmer ◽  
Kerstin Manke ◽  
Frank Schumacher ◽  
Fernando Cotinguiba ◽  
...  
Keyword(s):  
Substrate Specificity ◽  
Gene Family ◽  
Traditional Medicine ◽  
Aromatic Amines ◽  
Black Pepper ◽  
Piper Nigrum ◽  
Rna Seq ◽  
Enzymatic Formation ◽  
Identification And Characterization

AbstractBlack pepper (Piper nigrum L.) is the world’s most popular spice and is also used as an ingredient in traditional medicine. Its pungent perception is due to the interaction of its major compound, piperine (1-piperoyl-piperidine) with the human TRPV-1 or vanilloid receptor. We now identify the hitherto concealed enzymatic formation of piperine from piperoyl coenzyme A and piperidine based on a differential RNA-Seq approach from developing black pepper fruits. This enzyme is described as piperine synthase (piperoyl-CoA:piperidine piperoyl transferase) and is a member of the BAHD-type of acyltransferases encoded by a gene that is preferentially expressed in immature fruits. A second BAHD-type enzyme, also highly expressed in immature black pepper fruits, has a rather promiscuous substrate specificity, combining diverse CoA-esters with aliphatic and aromatic amines with similar efficiencies, and was termed piperamide synthase. Recombinant piperine and piperamide synthases are members of a small gene family in black pepper. They can be used to facilitate the microbial production of a broad range of medicinally relevant aliphatic and aromatic piperamides based on a wide array of CoA-donors and amine-derived acceptors, offering widespread applications.

Genome wide identification and characterization of abiotic stress responsive lncRNAs in Capsicum annuum

2021 ◽  
Author(s):  
Pooja Moni Baruah ◽  
Debasish B. Krishnatreya ◽  
Kuntala Sarma Bordoloi ◽  
Sarvajeet Singh Gill ◽  
Niraj Agarwala
Keyword(s):  
Abiotic Stress ◽  
Capsicum Annuum ◽  
Genome Wide ◽  
Identification And Characterization

scholarly journals Genome wide identification and characterization of light-harvesting Chloro a/b binding (LHC) genes reveals their potential role in enhancing drought tolerance in Gossypium hirsutum

2021 ◽  
Vol 4 (1) ◽  
Author(s):  
Teame Gereziher MEHARI ◽  
Yanchao XU ◽  
Richard Odongo MAGWANGA ◽  
Muhammad Jawad UMER ◽  
Joy Nyangasi KIRUNGU ◽  
...  
Keyword(s):  
Drought Stress ◽  
Gossypium Hirsutum ◽  
Abiotic Stresses ◽  
Substitution Rate ◽  
Synonymous Substitution ◽  
Synonymous Substitution Rate ◽  
Drought Tolerant ◽  
Genome Wide ◽  
Identification And Characterization

Abstract Background Cotton is an important commercial crop for being a valuable source of natural fiber. Its production has undergone a sharp decline because of abiotic stresses, etc. Drought is one of the major abiotic stress causing significant yield losses in cotton. However, plants have evolved self-defense mechanisms to cope abiotic factors like drought, salt, cold, etc. The evolution of stress responsive transcription factors such as the trihelix, a nodule-inception-like protein (NLP), and the late embryogenesis abundant proteins have shown positive response in the resistance improvement to several abiotic stresses. Results Genome wide identification and characterization of the effects of Light-Harvesting Chloro a/b binding (LHC) genes were carried out in cotton under drought stress conditions. A hundred and nine proteins encoded by the LHC genes were found in the cotton genome, with 55, 27, and 27 genes found to be distributed in Gossypium hirsutum, G. arboreum, and G. raimondii, respectively. The proteins encoded by the genes were unevenly distributed on various chromosomes. The Ka/Ks (Non-synonymous substitution rate/Synonymous substitution rate) values were less than one, an indication of negative selection of the gene family. Differential expressions of genes showed that majority of the genes are being highly upregulated in the roots as compared with leaves and stem tissues. Most genes were found to be highly expressed in MR-85, a relative drought tolerant germplasm. Conclusion The results provide proofs of the possible role of the LHC genes in improving drought stress tolerance, and can be explored by cotton breeders in releasing a more drought tolerant cotton varieties.

scholarly journals Genome-Wide Identification and Characterization of Hsf and Hsp Gene Families and Gene Expression Analysis under Heat Stress in Eggplant (Solanum melongema L.)

Horticulturae ◽  
2021 ◽  
Vol 7 (6) ◽  
pp. 149
Author(s):  
Chao Gong ◽  
Qiangqiang Pang ◽  
Zhiliang Li ◽  
Zhenxing Li ◽  
Riyuan Chen ◽  
...  
Keyword(s):  
Heat Stress ◽  
Gene Families ◽  
High Temperature Stress ◽  
Pcr Analysis ◽  
Rna Seq ◽  
Genome Wide ◽  
Motif Composition ◽  
Hsp Genes ◽  
Identification And Characterization

Under high temperature stress, a large number of proteins in plant cells will be denatured and inactivated. Meanwhile Hsfs and Hsps will be quickly induced to remove denatured proteins, so as to avoid programmed cell death, thus enhancing the thermotolerance of plants. Here, a comprehensive identification and analysis of the Hsf and Hsp gene families in eggplant under heat stress was performed. A total of 24 Hsf-like genes and 117 Hsp-like genes were identified from the eggplant genome using the interolog from Arabidopsis. The gene structure and motif composition of Hsf and Hsp genes were relatively conserved in each subfamily in eggplant. RNA-seq data and qRT-PCR analysis showed that the expressions of most eggplant Hsf and Hsp genes were increased upon exposure to heat stress, especially in thermotolerant line. The comprehensive analysis indicated that different sets of SmHsps genes were involved downstream of particular SmHsfs genes. These results provided a basis for revealing the roles of SmHsps and SmHsp for thermotolerance in eggplant, which may potentially be useful for understanding the thermotolerance mechanism involving SmHsps and SmHsp in eggplant.

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