scholarly journals Single-cell lineage tracking analysis reveals that an established cell line comprises putative cancer stem cells and their heterogeneous progeny

2016 ◽  
Vol 6 (1) ◽  
Author(s):  
Sachiko Sato ◽  
Ann Rancourt ◽  
Yukiko Sato ◽  
Masahiko S. Satoh
Keyword(s):  
Stem Cells ◽  
Cancer Stem Cells ◽  
Single Cell ◽  
Cell Line ◽  
Cell Lineage ◽  
Established Cell Line ◽  
Established Cell ◽  
Lineage Tracking

scholarly journals Identification of SNA-I-positive cells as stem-like cells in an established cell line using computerized single-cell lineage tracking

2018 ◽  
Author(s):  
Sachiko Sato ◽  
Ann Rancourt ◽  
Masahiko S. Satoh
Keyword(s):  
Single Cell ◽  
Cell Line ◽  
Cell Population ◽  
Cell Tracking ◽  
Cell Lineage ◽  
Live Cell ◽  
Wide Range ◽  
A Cell ◽  
Analysis System ◽  
Lineage Tracking

AbstractSingle-cell tracking analysis is a potential research technique for the accurate investigation of cellular behaviors and events occurring within a cell population. However, this analysis is challenging because of a lack of microscope hardware and software suitable for single-cell tracking analysis of a wide range of cell types and densities. We therefore developed a computerized single-cell lineage tracking analysis system based on a microscope optimized for differential interference contrast-based long-term live cell imaging, with software designed to automatically generate live cell videos, perform image segmentation, carry out single-cell tracking, and create and analyze a cell lineage database. We previously reported that minor cell sub-populations (3%–7%) within a cultured cancer cell line could play a critical role in maintaining the cell population. Given that sub-population characterization requires large-scale single-cell tracking analysis, we tracked single cells using the above computerized system and identified a minor cell population (1.5%) composed ofSambucus nigraagglutinin-I-positive cells, which acted as stem-like cells for the established culture. These results demonstrate the potential value of this computerized single-cell lineage tracking analysis system as a routine tool in cell biology, opening new avenues for research aimed at identifying previously unknown characteristics of individual cultured cells with high accuracy.

In vitro Three Dimensional Scaffold-free Construct of Human Adipose-derived Stem Cells in Coculture with Endothelial Cells and Fibroblasts

2017 ◽  
Vol 68 (6) ◽  
pp. 1341-1344
Author(s):  
Grigore Berea ◽  
Gheorghe Gh. Balan ◽  
Vasile Sandru ◽  
Paul Dan Sirbu
Keyword(s):  
Tissue Engineering ◽  
Stem Cells ◽  
Endothelial Cells ◽  
Single Cell ◽  
Cell Line ◽  
Bone Repair ◽  
Umbilical Vein ◽  
Human Fibroblasts ◽  
Three Dimensional ◽  
Adipose Derived Stem Cells

Complex interactions between stem cells, vascular cells and fibroblasts represent the substrate of building microenvironment-embedded 3D structures that can be grafted or added to bone substitute scaffolds in tissue engineering or clinical bone repair. Human Adipose-derived Stem Cells (hASCs), human umbilical vein endothelial cells (HUVECs) and normal dermal human fibroblasts (NDHF) can be mixed together in three dimensional scaffold free constructs and their behaviour will emphasize their potential use as seeding points in bone tissue engineering. Various combinations of the aforementioned cell lines were compared to single cell line culture in terms of size, viability and cell proliferation. At 5 weeks, viability dropped for single cell line spheroids while addition of NDHF to hASC maintained the viability at the same level at 5 weeks Fibroblasts addition to the 3D construct of stem cells and endothelial cells improves viability and reduces proliferation as a marker of cell differentiation toward osteogenic line.

Transport of L-carnitine induced by prednisolone in an established cell line (CCL 27)

1979 ◽  
Vol 585 (1) ◽  
pp. 94-99 ◽  
Author(s):  
Per Mølstad ◽  
Thomas Bøhmer
Keyword(s):  
Cell Line ◽  
Established Cell Line ◽  
Established Cell

A New Small RNA Virus Persistently Infecting an Established Cell Line ofGalleria mellonella,Induced by a Heterologous Infection

1997 ◽  
Vol 69 (1) ◽  
pp. 7-13 ◽  
Author(s):  
X. Lery ◽  
G. Fediere ◽  
A. Taha ◽  
M. Salah ◽  
J. Giannotti
Keyword(s):  
Cell Line ◽  
Small Rna ◽  
Rna Virus ◽  
Established Cell Line ◽  
Established Cell

scholarly journals Identification of leukemic and pre-leukemic stem cells by clonal tracking from single-cell transcriptomics

2021 ◽  
Vol 12 (1) ◽  
Author(s):  
Lars Velten ◽  
Benjamin A. Story ◽  
Pablo Hernández-Malmierca ◽  
Simon Raffel ◽  
Daniel R. Leonce ◽  
...  
Keyword(s):  
Gene Expression ◽  
Stem Cells ◽  
Cancer Stem Cells ◽  
Single Cell ◽  
Lineage Tracing ◽  
Specific Gene ◽  
Leukemic Stem Cells ◽  
Hematopoietic Stem ◽  
Mutational Status

AbstractCancer stem cells drive disease progression and relapse in many types of cancer. Despite this, a thorough characterization of these cells remains elusive and with it the ability to eradicate cancer at its source. In acute myeloid leukemia (AML), leukemic stem cells (LSCs) underlie mortality but are difficult to isolate due to their low abundance and high similarity to healthy hematopoietic stem cells (HSCs). Here, we demonstrate that LSCs, HSCs, and pre-leukemic stem cells can be identified and molecularly profiled by combining single-cell transcriptomics with lineage tracing using both nuclear and mitochondrial somatic variants. While mutational status discriminates between healthy and cancerous cells, gene expression distinguishes stem cells and progenitor cell populations. Our approach enables the identification of LSC-specific gene expression programs and the characterization of differentiation blocks induced by leukemic mutations. Taken together, we demonstrate the power of single-cell multi-omic approaches in characterizing cancer stem cells.

Biological Response of Advanced Glycation End Products to the Established Cell Line and Proteome Analysis

2005 ◽  
Vol 1043 (1) ◽  
pp. 908-908
Author(s):  
S OMURA ◽  
A TAYA ◽  
S MASUDA ◽  
H NAITOU ◽  
N OHASHI ◽  
...  
Keyword(s):  
Cell Line ◽  
Advanced Glycation End Products ◽  
Biological Response ◽  
Proteome Analysis ◽  
Established Cell Line ◽  
Advanced Glycation ◽  
Glycation End Products ◽  
End Products ◽  
Established Cell

A newly established cell line derived from the human dental pulp.

1989 ◽  
Vol 65 (8) ◽  
pp. 187-190
Author(s):  
Yasuji RIKITAKE ◽  
Atsushige SATO
Keyword(s):  
Cell Line ◽  
Dental Pulp ◽  
Established Cell Line ◽  
Human Dental Pulp ◽  
Established Cell

scholarly journals Human Lung Cancer cell line SPC-A1 contains cells with characteristics of cancer stem cells

Neoplasma ◽  
2012 ◽  
Vol 59 (06) ◽  
pp. 685-692 ◽  
Author(s):  
C. H. ZHOU ◽  
S. F. YANG ◽  
P. Q. LI
Keyword(s):  
Lung Cancer ◽  
Stem Cells ◽  
Cancer Stem Cells ◽  
Cell Line ◽  
Cancer Cell ◽  
Human Lung ◽  
Cancer Cell Line ◽  
Lung Cancer Cell Line ◽  
Human Lung Cancer ◽  
Lung Cancer Cell
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