The impact of zinc oxide nanoparticles on the bacterial microbiome of activated sludge systems

Zinc oxide nanoparticles (ZnO NPs) are among nanoscale materials, attracting increasing attention owing to their exceptional set of characteristics, which makes these engineered nanoparticles a great option for improving the quality and effectiveness of diagnosis and treatment. The capacity of ZnO NPs to induce reactive oxygen species (ROS) production, DNA damage, and apoptosis represents a promise for their use in both cancer therapy and microbial treatment. However, their intrinsic toxicity together with their easy entrance and accumulation in organism have raised some concerns regarding the biomedical use of these NPs. Several studies have reported that ZnO NPs might induce cytotoxic effects on the male reproductive system, compromising male fertility. Despite some advances in this area, the knowledge of the effects of ZnO NPs on male fertility is still scarce. Overall, a brief outline of the major ZnO NPs biomedical applications and promises in terms of diagnostic and therapeutic use will also be explored. Further, this review intends to discuss the effect of ZnO NPs exposure on the male reproductive system and speculate their effects on male (in)fertility.

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Investigation of Zinc Oxide Nanoparticles Effects on Removal of Total Coliform Bacteria in Activated Sludge Process Effluent of Municipal Wastewater

Journal of Environmental Science and Technology ◽

10.3923/jest.2017.49.55 ◽

2016 ◽

Vol 10 (1) ◽

pp. 49-55

Author(s):

Gholamreza Mostafaii ◽

Elahe Chimehi ◽

HamidReza Gilasi ◽

Leila Iranshahi

Keyword(s):

Zinc Oxide ◽

Activated Sludge ◽

Zinc Oxide Nanoparticles ◽

Municipal Wastewater ◽

Total Coliform ◽

Coliform Bacteria ◽

Oxide Nanoparticles ◽

Activated Sludge Process ◽

Total Coliform Bacteria

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Chitosan oligomer and zinc oxide nanoparticles for treating wastewaters: US20190134086 patent evaluation

Recent Patents on Biotechnology ◽

10.2174/1872208315666211026104828 ◽

2021 ◽

Vol 15 ◽

Author(s):

Fernanda Guimarães Valverde ◽

Daniela Droppa-Almeida ◽

Francine Ferreira Padilha

Keyword(s):

Zinc Oxide ◽

Zinc Oxide Nanoparticles ◽

Chitosan Nanoparticles ◽

Wastewater Reuse ◽

The United States ◽

Green Energy ◽

Resistant Bacteria ◽

Oxide Nanoparticles ◽

Alternative Source ◽

The Impact

: With the utilisation of algae, wastewater reuse is becoming a viable option for the energy industry, especially green energy. The growth of these algae in these wastewaters provides an alternative source for bioenergetics, however, the growth of other microorganisms can directly affect the production of bioenergy, requiring the removal and reduction of contaminants in these waters, in addition to being a source of contamination for workers. Therefore, the use of nanoparticles in bioremediation has been an alternative to mitigate the contamination of these wastewaters that have microorganisms capable of reducing the algae growth capacity. The objective of this work was to verify in the United States Patent and Trademarker office database (USPTO) patents that used chitosan nanoparticles as a form of wastewater treatment and to carry out the analysis of patent US20190134086, which addresses the use of zinc oxide nanoparticles associated with chitosan that was developed and used to evaluate their antibacterial activity against resistant microorganisms and biofilm producers present in wastewater. Escherichia coli, Enterococcus faecium, and/or Pseudomonas aeruginosa are the microorganisms involved in the evaluated invention, bacteria present in the gastrointestinal tract, of clinical and environmental importance. The synthesized nanoparticles are arranged as a pharmaceutically acceptable and toxic vehicle against resistant bacteria, thus being described as nanoremediators. Given the analyzed patent, it was possible to verify the importance of alternatives to reduce the impact that pollution, in general, has on the environment, in addition to the proposed technology serving to maintain the survival and development capacity of the algae that will be able to produce green energy, the nanoparticles with antibacterial potential can help indirectly reduce these pathogenic strains with resistance to several antibiotics in the environment.

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Assessing the Impact of Titanium Dioxide and Zinc Oxide Nanoparticles on Bacteria Using a Fluorescent-Based Cell Membrane Integrity Assay

Environmental Engineering Science ◽

10.1089/ees.2009.0332 ◽

2010 ◽

Vol 27 (4) ◽

pp. 329-335 ◽

Cited By ~ 23

Author(s):

Olga N. Mileyeva-Biebesheimer ◽

Amr Zaky ◽

Cyndee L. Gruden

Keyword(s):

Zinc Oxide ◽

Titanium Dioxide ◽

Cell Membrane ◽

Zinc Oxide Nanoparticles ◽

Membrane Integrity ◽

Oxide Nanoparticles ◽

Cell Membrane Integrity ◽

The Impact

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Ameliorative Effect of Zinc Oxide Nanoparticles on Antioxidants and Sperm Characteristics in Streptozotocin-Induced Diabetic Rat Testes

BioMed Research International ◽

10.1155/2015/153573 ◽

2015 ◽

Vol 2015 ◽

pp. 1-6 ◽

Cited By ~ 48

Author(s):

Mohamed Afifi ◽

Omar A. Almaghrabi ◽

Naif Mohammed Kadasa

Keyword(s):

Zinc Oxide ◽

Mrna Expression ◽

Zinc Oxide Nanoparticles ◽

Sperm Count ◽

Testicular Tissue ◽

Diabetic Rats ◽

Diabetic Rat ◽

Oxide Nanoparticles ◽

Sperm Characteristics ◽

The Impact

The present study investigated the impact of zinc oxide nanoparticles (ZnONPs) on the oxidative status and sperm characteristics in diabetic rat testicular tissue. Forty male albino rats were used in this study; 10 of them served as a control and 30 rats were injected with a single dose (100 mg/kg) of streptozotocin intraperitoneally. They were subdivided into diabetic, diabetic + ZnONPs (10 mg/kg B.W.), and diabetic and cotreated with ZnONPs + insulin groups. The sperm count and motility were assessed. The activity and mRNA expression of superoxide dismutase (SOD), catalase (CAT), glutathione peroxidase (GPx), glutathione reductase (GRD), and Glutathion-S-Transferase (GST) were determined in the testicular tissue. Malondialdehyde (MDA) and reduced glutathione (GSH) levels were estimated in the testicular tissue. Sperm count and motility increased in ZnONPs treated diabetic rats. A significant increase in the activity and mRNA expression of SOD, CAT, GPx, GRD, and GST was shown in ZnONPs treated diabetic rats. MDA significantly decreased, while GSH increased in testicular tissue of ZnONPs treated diabetic rats. It was concluded that ZnONPs either alone or in combination with insulin have the ability to increase the sperm count and motility and protect the testicular tissue against the oxidative stress induced by diabetes in rats.

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Long-Term Impact of Zinc Oxide Nanoparticles on Differentiation and Cytokine Secretion of Human Adipose-Derived Stromal Cells

Materials ◽

10.3390/ma12111823 ◽

2019 ◽

Vol 12 (11) ◽

pp. 1823 ◽

Cited By ~ 2

Author(s):

Katrin Radeloff ◽

Andreas Radeloff ◽

Mario Ramos Tirado ◽

Agmal Scherzad ◽

Rudolf Hagen ◽

...

Keyword(s):

Gene Expression ◽

Zinc Oxide ◽

Stromal Cells ◽

Zinc Oxide Nanoparticles ◽

Caspase 3 ◽

Cytokine Secretion ◽

Oxide Nanoparticles ◽

Zno Nps ◽

The Impact

Zinc oxide nanoparticles (ZnO-NPs) are widely utilized, for example in manufacturing paints and in the cosmetic industry. In addition, there is raising interest in the application of NPs in stem cell research. However, cytotoxic, genotoxic and pro-inflammatory effects were shown for NPs. The aim of this study was to evaluate the impact of ZnO-NPs on cytokine secretion and differentiation properties of human adipose tissue-derived stromal cells (ASCs). Human ASCs were exposed to the subtoxic concentration of 0.2 µg/mL ZnO-NPs for 24 h. After four weeks of cultivation, adipogenic and osteogenic differentiation procedures were performed. The multi-differentiation potential was confirmed histologically and using polymerase chain reaction (PCR). In addition, the gene expression of IL-6, IL-8, vascular endothelial growth factor (VEGF) and caspase 3 was analyzed. Over the course of four weeks after ZnO-NPs exposure, no significant differences were detected in the gene expression of IL-6, IL-8, VEGF and caspase 3 compared to non-exposed cells. The differentiation was also not affected by the ZnO-NPs. These findings underline the fact, that functionality of ASCs is likely to be unaffected by ZnO-NPs, despite a long-term disposition of NPs in the cells, supposing that the starting concentration was safely in the non-toxic range. This might provide important information for single-use nanomedical applications of ZnO-NPs.

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Effects of Biogenic Zinc Oxide Nanoparticles on Growth and Oxidative Stress Response in Flax Seedlings vs. In Vitro Cultures: A Comparative Analysis

Biomolecules ◽

10.3390/biom10060918 ◽

2020 ◽

Vol 10 (6) ◽

pp. 918 ◽

Cited By ~ 1

Author(s):

Afifa Zaeem ◽

Samantha Drouet ◽

Sumaira Anjum ◽

Razia Khurshid ◽

Muhammad Younas ◽

...

Keyword(s):

Oxidative Stress ◽

Zinc Oxide ◽

Zinc Oxide Nanoparticles ◽

In Vitro Cultures ◽

Total Phenolic ◽

Oxide Nanoparticles ◽

Stem Explants ◽

Enhanced Production ◽

The Impact

Linum usitatissimum biosynthesizes lignans and neolignans that are diet and medicinally valuable metabolites. In recent years, zinc oxide nanoparticles (ZnONPs) have emerged as potential elicitors for the enhanced biosynthesis of commercial secondary metabolites. Herein, we investigated the influence of biogenic ZnONPs on both seedlings and stem-derived callus of L. usitatissimum. Seedlings of L. usitatissimum grown on Murashige and Skoog (MS) medium supplemented with ZnONPs (1–1000 mg/L) presented the highest antioxidant activity, total phenolic content, total flavonoid content, peroxidase and superoxide dismutase activities at 500 mg/L, while the maximum plantlet length was achieved with 10 mg/L. Likewise, the high-performance liquid chromatography (HPLC) analysis revealed the enhanced production of secoisolariciresinol diglucoside, lariciresinol diglucoside, dehydrodiconiferyl alcohol glucoside and guaiacylglycerol-β-coniferyl alcohol ether glucoside in the plantlets grown on the 500 mg/L ZnONPs. On the other hand, the stem explants were cultured on MS media comprising 1-naphthaleneacetic acid (1 mg/L) and ZnONPs (1–50 mg/L). The highest antioxidant and other activities with an enhanced rooting effect were noted in 25 mg/L ZnONP-treated callus. Similarly, the maximum metabolites were also accumulated in 25 mg/L ZnONP-treated callus. In both systems, the dose-dependent production of reactive oxygen species (ROS) was recorded, resulting in oxidative damage with a more pronounced toxic effect on in vitro cultures. Altogether, the results from this study constitute a first comprehensive view of the impact of ZnONPs on the oxidative stress and antioxidant responses in seedlings vs. in vitro cultures.

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Iron-doped zinc oxide nanoparticles-triggered elicitation of important phenolic compounds in cell cultures of Fagonia indica.

10.21203/rs.3.rs-314389/v1 ◽

2021 ◽

Author(s):

Atta Ullah Khan ◽

Tariq Khan ◽

Mubarak Ali Khan ◽

Akhtar Nadhman ◽

Muhammad Aasim ◽

...

Keyword(s):

Zinc Oxide ◽

Secondary Metabolism ◽

Zinc Oxide Nanoparticles ◽

Biomass Accumulation ◽

Callus Cultures ◽

Oxide Nanoparticles ◽

Stem Explants ◽

Zno Nps ◽

Iron Doped ◽

The Impact

Abstract Fagonia indica is an important medicinal plant species used traditionally against a variety of diseases. In this study, we initiated callus cultures from healthy stem explants. We observed maximum callus induction frequency (88%) on MS media supplemented with Thidiazuron (1.0 mg/mL). We also examined the callus cultures to determine the impact of iron-doped zinc oxide nanoparticles (Fe-ZnO-NPs) in concentrations (15.62 to 250 µg/mL) on biomass accumulation, secondary metabolism, and antioxidative potential in callus cultures of F. indica. Our results showed that maximum callus biomass (FW = 13.6 g and DW = 0.58 ± 0.01) was produced on day 40 when the media was supplemented with 250 µg/mL Fe-ZnO-NPs. Similarly, maximum TPC (268.36 µg GAE/g of DW) was detected in 40 days old callus added with 125 µg/mL Fe-ZnO-NPs. Maximum TFC (78.56 µg QE/g of DW) was observed in 20 days old callus grown in 62.5 µg/mL Fe-ZnO-NPs containing media. Maximum total antioxidant capacity (390.74 µg AAE/g of DW) was observed in 40 days old callus with 125 µg/mL Fe-ZnO-NPs treated cultures, respectively. The antioxidant potential was in correlation with the TPC. These results showed that Fe-ZnO-NPs elicitors can increase the biomass and activate secondary metabolism in F. indica cells.

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