The effect of filter material on bioaerosol collection of Bacillus subtilis spores used as a Bacillus anthracis simulant

2005 ◽  
Vol 7 (5) ◽  
pp. 475 ◽  
Author(s):  
Nancy Clark Burton ◽  
Atin Adhikari ◽  
Sergey A. Grinshpun ◽  
Richard Hornung ◽  
Tiina Reponen
Keyword(s):  
Bacillus Subtilis ◽  
Bacillus Anthracis ◽  
Filter Material

scholarly journals Decontamination assessment of Bacillus anthracis, Bacillus subtilis, and Geobacillus stearothermophilus spores on indoor surfaces using a hydrogen peroxide gas generator

2005 ◽  
Vol 99 (4) ◽  
pp. 739-748 ◽  
Author(s):  
J.V. Rogers ◽  
C.L.K. Sabourin ◽  
Y.W. Choi ◽  
W.R. Richter ◽  
D.C. Rudnicki ◽  
...  
Keyword(s):  
Hydrogen Peroxide ◽  
Bacillus Subtilis ◽  
Bacillus Anthracis ◽  
Geobacillus Stearothermophilus ◽  
Gas Generator

scholarly journals The differential effects of heat‐shocking on the viability of spores from Bacillus anthracis , Bacillus subtilis , and Clostridium sporogenes after treatment with peracetic acid‐ and glutaraldehyde‐based disinfectants

2015 ◽  
Vol 4 (5) ◽  
pp. 764-773 ◽  
Author(s):  
Jordon K. March ◽  
Michael D. Pratt ◽  
Chinn‐Woan Lowe ◽  
Marissa N. Cohen ◽  
Benjamin A. Satterfield ◽  
...  
Keyword(s):  
Bacillus Subtilis ◽  
Bacillus Anthracis ◽  
Peracetic Acid ◽  
Clostridium Sporogenes ◽  
Differential Effects

scholarly journals Environmental Persistence of Bacillus anthracis and Bacillus subtilis Spores

PLoS ONE ◽  
2015 ◽  
Vol 10 (9) ◽  
pp. e0138083 ◽  
Author(s):  
Joseph P. Wood ◽  
Kathryn M. Meyer ◽  
Thomas J. Kelly ◽  
Young W. Choi ◽  
James V. Rogers ◽  
...  
Keyword(s):  
Bacillus Subtilis ◽  
Bacillus Anthracis ◽  
Environmental Persistence

scholarly journals Proteomic Analysis of the Spore Coats of Bacillus subtilis and Bacillus anthracis

2003 ◽  
Vol 185 (4) ◽  
pp. 1443-1454 ◽  
Author(s):  
Erh-Min Lai ◽  
Nikhil D. Phadke ◽  
Maureen T. Kachman ◽  
Rebecca Giorno ◽  
Santiago Vazquez ◽  
...  
Keyword(s):  
Bacillus Subtilis ◽  
Bacillus Anthracis ◽  
Proteomic Analysis ◽  
Polyacrylamide Gel Electrophoresis ◽  
Sodium Dodecyl ◽  
Detection Methods ◽  
Coat Proteins ◽  
Bona Fide ◽  
Spore Proteins ◽  
Spore Coat Proteins

ABSTRACT The outermost proteinaceous layer of bacterial spores, called the coat, is critical for spore survival, germination, and, for pathogenic spores, disease. To identify novel spore coat proteins, we have carried out a preliminary proteomic analysis of Bacillus subtilis and Bacillus anthracis spores, using a combination of standard sodium dodecyl sulfate-polyacrylamide gel electrophoresis separation and improved two-dimensional electrophoretic separations, followed by matrix-assisted laser desorption ionization-time of flight and/or dual mass spectrometry. We identified 38 B. subtilis spore proteins, 12 of which are known coat proteins. We propose that, of the novel proteins, YtaA, YvdP, and YnzH are bona fide coat proteins, and we have renamed them CotI, CotQ, and CotU, respectively. In addition, we initiated a study of coat proteins in B. anthracis and identified 11 spore proteins, 6 of which are candidate coat or exosporium proteins. We also queried the unfinished B. anthracis genome for potential coat proteins. Our analysis suggests that the B. subtilis and B. anthracis coats have roughly similar numbers of proteins and that a core group of coat protein species is shared between these organisms, including the major morphogenetic proteins. Nonetheless, a significant number of coat proteins are probably unique to each species. These results should accelerate efforts to develop B. anthracis detection methods and understand the ecological role of the coat.

scholarly journals Flocculating Properties of a Bioflocculant Purified from Bacillus Subtilis Isolated from the Stream Sediments of Onyearugbulem Market, Akure, Nigeria

2019 ◽  
pp. 1-16
Author(s):  
F. O. Ekundayo ◽  
F. B. Omiyale ◽  
E. R. Omomo
Keyword(s):  
Bacillus Subtilis ◽  
Metal Ions ◽  
Bacillus Anthracis ◽  
Streptomyces Griseus ◽  
Stream Sediments ◽  
Soil Samples ◽  
Bacterial Isolates ◽  
Potential Source ◽  
Flocculating Activity ◽  
Ondo State

Soil samples (sediments of stream, its bank and abattoir soil) were collected from Onyearugbulem market abattoir, Akure, Ondo State, Nigeria. Bacteria were isolated from the above soil samples by dilution and pour plate methods. Screening for best bioflocculating bacteria was also performed. Effects of metal ions (such as Mg2+, Ca2+ and Al3+), temperature and pH on flocculating activities of the bioflocculant were also determined. Six bacterial isolates producing flocculating substances were isolated and the isolate with the best flocculating property was selected. The identified bioflocculant producing bacteria are Bacillus anthracis, B. subtilis, B. thuringiensis, B. cereus, Streptomyces griseus and S. somaliensis. The best bioflocculant producing bacterium was Bacillus subtilis and the flocculating activity of its bioflocculant was stimulated in the presence of Mg2+, Ca2+and Al3+. This bioflocculant was thermostable and retained more than 80% of its flocculating activity after being heated at 100ºC for 25 minutes. It had the highest flocculating activity of 85% at pH 6 with optimum bioflocculant dosage of 0.8 mL. This study suggests soil samples from Onyearugbulem market abattoir as a potential source of bioflocculant-producing bacteria with good bioflocculating properties.

scholarly journals Bacterial spore detection and analysis using hyperpolarized 129Xe chemical exchange saturation transfer (Hyper-CEST) NMR

2014 ◽  
Vol 5 (8) ◽  
pp. 3197-3203 ◽  
Author(s):  
Yubin Bai ◽  
Yanfei Wang ◽  
Mark Goulian ◽  
Adam Driks ◽  
Ivan J. Dmochowski
Keyword(s):  
Bacillus Subtilis ◽  
Nmr Spectroscopy ◽  
Bacillus Anthracis ◽  
Chemical Exchange ◽  
Bacterial Spore ◽  
Chemical Exchange Saturation Transfer ◽  
Saturation Transfer ◽  
Hyperpolarized 129Xe ◽  
Spore Detection

Hyper-CEST 129Xe NMR spectroscopy was employed to detect Bacillus anthracis and Bacillus subtilis spores in solution, and interrogate the layers that comprise their structures.

scholarly journals The use of ozone gas for the inactivation of Bacillus anthracis and Bacillus subtilis spores on building materials

PLoS ONE ◽  
2020 ◽  
Vol 15 (5) ◽  
pp. e0233291
Author(s):  
Joseph P. Wood ◽  
Morgan Wendling ◽  
William Richter ◽  
James Rogers
Keyword(s):  
Bacillus Subtilis ◽  
Bacillus Anthracis ◽  
Building Materials

scholarly journals Poly-γ-Glutamate Capsule-Degrading Enzyme Treatment Enhances Phagocytosis and Killing of Encapsulated Bacillus anthracis

2006 ◽  
Vol 51 (1) ◽  
pp. 215-222 ◽  
Author(s):  
Angelo Scorpio ◽  
Donald J. Chabot ◽  
William A. Day ◽  
David K. O'Brien ◽  
Nicholas J. Vietri ◽  
...  
Keyword(s):  
Bacillus Subtilis ◽  
Glutamic Acid ◽  
Bacillus Anthracis ◽  
Enzyme Treatment ◽  
Human Neutrophils ◽  
Acid Hydrolase ◽  
Polyglutamic Acid ◽  
New Approach ◽  
Macrophage Phagocytosis ◽  
Phagocytic Killing

ABSTRACT The poly-γ-d-glutamic acid capsule confers antiphagocytic properties on Bacillus anthracis and is essential for virulence. In this study, we showed that CapD, a γ-polyglutamic acid depolymerase encoded on the B. anthracis capsule plasmid, degraded purified capsule and removed the capsule from the surface of anthrax bacilli. Treatment with CapD induced macrophage phagocytosis of encapsulated B. anthracis and enabled human neutrophils to kill encapsulated organisms. A second glutamylase, PghP, a γ-polyglutamic acid hydrolase encoded by Bacillus subtilis bacteriophage ΦNIT1, had minimal activity in degrading B. anthracis capsule, no effect on macrophage phagocytosis, and only minimal enhancement of neutrophil killing. Thus, the levels of both phagocytosis and killing corresponded to the degree of enzyme-mediated capsule degradation. The use of enzymes to degrade the capsule and enable phagocytic killing of B. anthracis offers a new approach to the therapy of anthrax.

scholarly journals Lytic enzyme-assisted germination of Bacillus anthracis and Bacillus subtilis spores

2015 ◽  
Vol 119 (2) ◽  
pp. 521-528 ◽  
Author(s):  
B.G. Blankenship ◽  
J.D. Heffron ◽  
D.L. Popham
Keyword(s):  
Bacillus Subtilis ◽  
Bacillus Anthracis ◽  
Lytic Enzyme
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