scholarly journals Lytic enzyme-assisted germination of Bacillus anthracis and Bacillus subtilis spores

2015 ◽  
Vol 119 (2) ◽  
pp. 521-528 ◽  
Author(s):  
B.G. Blankenship ◽  
J.D. Heffron ◽  
D.L. Popham
Keyword(s):  
Bacillus Subtilis ◽  
Bacillus Anthracis ◽  
Lytic Enzyme

scholarly journals Decontamination assessment of Bacillus anthracis, Bacillus subtilis, and Geobacillus stearothermophilus spores on indoor surfaces using a hydrogen peroxide gas generator

2005 ◽  
Vol 99 (4) ◽  
pp. 739-748 ◽  
Author(s):  
J.V. Rogers ◽  
C.L.K. Sabourin ◽  
Y.W. Choi ◽  
W.R. Richter ◽  
D.C. Rudnicki ◽  
...  
Keyword(s):  
Hydrogen Peroxide ◽  
Bacillus Subtilis ◽  
Bacillus Anthracis ◽  
Geobacillus Stearothermophilus ◽  
Gas Generator

The effect of filter material on bioaerosol collection of Bacillus subtilis spores used as a Bacillus anthracis simulant

2005 ◽  
Vol 7 (5) ◽  
pp. 475 ◽  
Author(s):  
Nancy Clark Burton ◽  
Atin Adhikari ◽  
Sergey A. Grinshpun ◽  
Richard Hornung ◽  
Tiina Reponen
Keyword(s):  
Bacillus Subtilis ◽  
Bacillus Anthracis ◽  
Filter Material

scholarly journals The differential effects of heat‐shocking on the viability of spores from Bacillus anthracis , Bacillus subtilis , and Clostridium sporogenes after treatment with peracetic acid‐ and glutaraldehyde‐based disinfectants

2015 ◽  
Vol 4 (5) ◽  
pp. 764-773 ◽  
Author(s):  
Jordon K. March ◽  
Michael D. Pratt ◽  
Chinn‐Woan Lowe ◽  
Marissa N. Cohen ◽  
Benjamin A. Satterfield ◽  
...  
Keyword(s):  
Bacillus Subtilis ◽  
Bacillus Anthracis ◽  
Peracetic Acid ◽  
Clostridium Sporogenes ◽  
Differential Effects

scholarly journals Environmental Persistence of Bacillus anthracis and Bacillus subtilis Spores

PLoS ONE ◽  
2015 ◽  
Vol 10 (9) ◽  
pp. e0138083 ◽  
Author(s):  
Joseph P. Wood ◽  
Kathryn M. Meyer ◽  
Thomas J. Kelly ◽  
Young W. Choi ◽  
James V. Rogers ◽  
...  
Keyword(s):  
Bacillus Subtilis ◽  
Bacillus Anthracis ◽  
Environmental Persistence

scholarly journals Proteomic Analysis of the Spore Coats of Bacillus subtilis and Bacillus anthracis

2003 ◽  
Vol 185 (4) ◽  
pp. 1443-1454 ◽  
Author(s):  
Erh-Min Lai ◽  
Nikhil D. Phadke ◽  
Maureen T. Kachman ◽  
Rebecca Giorno ◽  
Santiago Vazquez ◽  
...  
Keyword(s):  
Bacillus Subtilis ◽  
Bacillus Anthracis ◽  
Proteomic Analysis ◽  
Polyacrylamide Gel Electrophoresis ◽  
Sodium Dodecyl ◽  
Detection Methods ◽  
Coat Proteins ◽  
Bona Fide ◽  
Spore Proteins ◽  
Spore Coat Proteins

ABSTRACT The outermost proteinaceous layer of bacterial spores, called the coat, is critical for spore survival, germination, and, for pathogenic spores, disease. To identify novel spore coat proteins, we have carried out a preliminary proteomic analysis of Bacillus subtilis and Bacillus anthracis spores, using a combination of standard sodium dodecyl sulfate-polyacrylamide gel electrophoresis separation and improved two-dimensional electrophoretic separations, followed by matrix-assisted laser desorption ionization-time of flight and/or dual mass spectrometry. We identified 38 B. subtilis spore proteins, 12 of which are known coat proteins. We propose that, of the novel proteins, YtaA, YvdP, and YnzH are bona fide coat proteins, and we have renamed them CotI, CotQ, and CotU, respectively. In addition, we initiated a study of coat proteins in B. anthracis and identified 11 spore proteins, 6 of which are candidate coat or exosporium proteins. We also queried the unfinished B. anthracis genome for potential coat proteins. Our analysis suggests that the B. subtilis and B. anthracis coats have roughly similar numbers of proteins and that a core group of coat protein species is shared between these organisms, including the major morphogenetic proteins. Nonetheless, a significant number of coat proteins are probably unique to each species. These results should accelerate efforts to develop B. anthracis detection methods and understand the ecological role of the coat.

scholarly journals In Vitro Studies of Peptidoglycan Binding and Hydrolysis by the Bacillus anthracis Germination-Specific Lytic Enzyme SleB

2010 ◽  
Vol 193 (1) ◽  
pp. 125-131 ◽  
Author(s):  
J. D. Heffron ◽  
N. Sherry ◽  
D. L. Popham
Keyword(s):  
Bacillus Anthracis ◽  
In Vitro Studies ◽  
Lytic Enzyme

scholarly journals Role of Dipicolinic Acid in the Germination, Stability, and Viability of Spores of Bacillus subtilis

2008 ◽  
Vol 190 (14) ◽  
pp. 4798-4807 ◽  
Author(s):  
Anil Magge ◽  
Amanda C. Granger ◽  
Paul G. Wahome ◽  
Barbara Setlow ◽  
Venkata R. Vepachedu ◽  
...  
Keyword(s):  
Bacillus Subtilis ◽  
Small Molecules ◽  
Spore Germination ◽  
Dipicolinic Acid ◽  
Great Majority ◽  
Lytic Enzyme ◽  
Spore Proteins ◽  
Low Levels ◽  
Made In

ABSTRACT Spores of Bacillus subtilis spoVF strains that cannot synthesize dipicolinic acid (DPA) but take it up during sporulation were prepared in medium with various DPA concentrations, and the germination and viability of these spores as well as the DPA content in individual spores were measured. Levels of some other small molecules in DPA-less spores were also measured. These studies have allowed the following conclusions. (i) Spores with no DPA or low DPA levels that lack either the cortex-lytic enzyme (CLE) SleB or the receptors that respond to nutrient germinants could be isolated but were unstable and spontaneously initiated early steps in spore germination. (ii) Spores that lacked SleB and nutrient germinant receptors and also had low DPA levels were more stable. (iii) Spontaneous germination of spores with no DPA or low DPA levels was at least in part via activation of SleB. (iv) The other redundant CLE, CwlJ, was activated only by the release of high levels of DPA from spores. (v) Low levels of DPA were sufficient for the viability of spores that lacked most α/β-type small, acid-soluble spore proteins. (vi) DPA levels accumulated in spores prepared in low-DPA-containing media varied greatly between individual spores, in contrast to the presence of more homogeneous DPA levels in individual spores made in media with high DPA concentrations. (vii) At least the great majority of spores of several spoVF strains that contained no DPA also lacked other major spore small molecules and had gone through some of the early reactions in spore germination.

scholarly journals Flocculating Properties of a Bioflocculant Purified from Bacillus Subtilis Isolated from the Stream Sediments of Onyearugbulem Market, Akure, Nigeria

2019 ◽  
pp. 1-16
Author(s):  
F. O. Ekundayo ◽  
F. B. Omiyale ◽  
E. R. Omomo
Keyword(s):  
Bacillus Subtilis ◽  
Metal Ions ◽  
Bacillus Anthracis ◽  
Streptomyces Griseus ◽  
Stream Sediments ◽  
Soil Samples ◽  
Bacterial Isolates ◽  
Potential Source ◽  
Flocculating Activity ◽  
Ondo State

Soil samples (sediments of stream, its bank and abattoir soil) were collected from Onyearugbulem market abattoir, Akure, Ondo State, Nigeria. Bacteria were isolated from the above soil samples by dilution and pour plate methods. Screening for best bioflocculating bacteria was also performed. Effects of metal ions (such as Mg2+, Ca2+ and Al3+), temperature and pH on flocculating activities of the bioflocculant were also determined. Six bacterial isolates producing flocculating substances were isolated and the isolate with the best flocculating property was selected. The identified bioflocculant producing bacteria are Bacillus anthracis, B. subtilis, B. thuringiensis, B. cereus, Streptomyces griseus and S. somaliensis. The best bioflocculant producing bacterium was Bacillus subtilis and the flocculating activity of its bioflocculant was stimulated in the presence of Mg2+, Ca2+and Al3+. This bioflocculant was thermostable and retained more than 80% of its flocculating activity after being heated at 100ºC for 25 minutes. It had the highest flocculating activity of 85% at pH 6 with optimum bioflocculant dosage of 0.8 mL. This study suggests soil samples from Onyearugbulem market abattoir as a potential source of bioflocculant-producing bacteria with good bioflocculating properties.

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