scholarly journals Flocculating Properties of a Bioflocculant Purified from Bacillus Subtilis Isolated from the Stream Sediments of Onyearugbulem Market, Akure, Nigeria

2019 ◽  
pp. 1-16
Author(s):  
F. O. Ekundayo ◽  
F. B. Omiyale ◽  
E. R. Omomo
Keyword(s):  
Bacillus Subtilis ◽  
Metal Ions ◽  
Bacillus Anthracis ◽  
Streptomyces Griseus ◽  
Stream Sediments ◽  
Soil Samples ◽  
Bacterial Isolates ◽  
Potential Source ◽  
Flocculating Activity ◽  
Ondo State

Soil samples (sediments of stream, its bank and abattoir soil) were collected from Onyearugbulem market abattoir, Akure, Ondo State, Nigeria. Bacteria were isolated from the above soil samples by dilution and pour plate methods. Screening for best bioflocculating bacteria was also performed. Effects of metal ions (such as Mg2+, Ca2+ and Al3+), temperature and pH on flocculating activities of the bioflocculant were also determined. Six bacterial isolates producing flocculating substances were isolated and the isolate with the best flocculating property was selected. The identified bioflocculant producing bacteria are Bacillus anthracis, B. subtilis, B. thuringiensis, B. cereus, Streptomyces griseus and S. somaliensis. The best bioflocculant producing bacterium was Bacillus subtilis and the flocculating activity of its bioflocculant was stimulated in the presence of Mg2+, Ca2+and Al3+. This bioflocculant was thermostable and retained more than 80% of its flocculating activity after being heated at 100ºC for 25 minutes. It had the highest flocculating activity of 85% at pH 6 with optimum bioflocculant dosage of 0.8 mL. This study suggests soil samples from Onyearugbulem market abattoir as a potential source of bioflocculant-producing bacteria with good bioflocculating properties.

scholarly journals Poultry Farms as a Potential Source of Environmental Pollution by Pharmaceuticals

Molecules ◽  
2020 ◽  
Vol 25 (5) ◽  
pp. 1031 ◽  
Author(s):  
Katarzyna Wychodnik ◽  
Grażyna Gałęzowska ◽  
Justyna Rogowska ◽  
Marta Potrykus ◽  
Alina Plenis ◽  
...  
Keyword(s):  
High Performance ◽  
Bacterial Resistance ◽  
Soil Samples ◽  
Reference Area ◽  
Bacterial Isolates ◽  
Mass Spectrometry Detection ◽  
Veterinary Pharmaceuticals ◽  
Potential Source ◽  
Poultry Breeding ◽  
Increase Productivity

Industrial poultry breeding is associated with the need to increase productivity while maintaining low meat prices. Little is known about its impact on the environment of soil pollution by pharmaceuticals. Breeders routinely use veterinary pharmaceuticals for therapeutic and preventive purposes. The aim of this work was to determine the influence of mass breeding of hens on the soil contamination with 26 pharmaceuticals and caffeine. During two seasons—winter and summer 2019—15 soil samples were collected. Liquid extraction was used to isolate analytes from samples. Extracts were analyzed using ultra-high performance liquid chromatography coupled with tandem mass spectrometry detection (UPLC-MS/MS). The results showed the seasonal changes in pharmaceutical presence in analyzed soil samples. Ten pharmaceuticals (metoclopramide, sulphanilamide, salicic acid, metoprolol, sulphamethazine, nimesulide, carbamazepine, trimethoprim, propranolol, and paracetamol) and caffeine were determined in soil samples collected in March, and five pharmaceuticals (metoclopramide, sulphanilamide, sulphamethazine, carbamazepine, sulfanilamid) in soil samples collected in July. The highest concentrations were observed for sulphanilamide, in a range from 746.57 ± 15.61 ng/g d.w to 3518.22 ± 146.05 ng/g d.w. The level of bacterial resistance to antibiotics did not differ between samples coming from intensive breeding farm surroundings and the reference area, based on antibiotic resistance of 85 random bacterial isolates.

scholarly journals Degradative Ability of Silver Particles Synthesized by Gram-negative Bacteria of Some Crops Rhizosphere on Crude Oil Polluted Soil

2020 ◽  
pp. 25-33
Author(s):  
F. O. Ekundayo ◽  
D. B. Orisadipe ◽  
I. A. Onifade
Keyword(s):  
Crude Oil ◽  
Basal Medium ◽  
Polluted Soil ◽  
Soil Samples ◽  
Edwardsiella Tarda ◽  
Gram Negative Bacteria ◽  
Silver Particles ◽  
Bacterial Isolates ◽  
Gram Negative ◽  
Ondo State

Aim: To degrade the crude oil polluted soil with silver particles synthesized by some Gram negative rhizosphere bacteria of cocoa, orange and timber obtained from Idanre, Ondo State, Nigeria. Place and Duration of Study: Soil samples were obtained from Idanre, Ondo State while crude oil polluted soil samples were obtained from Zion town in Ese- Odo at Ilaje, Ondo State, between June and September 2018. Also, Bonnylight Crude oil was collected from Warri in Nigeria. This research work was carried out at the Department of Microbiology Laboratory, Federal University of Technology, Akure. Methodology: Soil samples (rhizosphere) were collected in sterile polythene bags and tightly packed and transported for analysis. The types and loads of bacteria present in the soil samples were determined. Identification and characterization of various bacterial isolates were based on Gram staining techniques and different biochemical tests. The physicochemical properties of the crude oil polluted soil were determined. The microbial biomass and the synthesis of silver particles were carried out while the bioremediation of the crude oil polluted soil with the silver particles which were performed in triplicate. Also, bioremediation of crude oil with the bacterial isolates were performed in triplicate. Five millilitres (5 ml) of silver particles produced was inoculated into 50 g of sterilized crude oil polluted soil in a plastic container with 5ml of basal medium and 3 ml of the harvested cells of the isolates was inoculated into 10 ml of the basal medium with 30ml of crude oil in a conical flask. The unsterilized soil without inoculum and silver particles were served as control, also crude oil without inoculum and silver nanoparticles were served as a control. Results: Citrobacter freundii, Yersinia pestis, Edwardsiella tarda, were isolated from rhizosphere of cocoa. Serratia marcescens, Providencia stuatii were isolated from rhizosphere of orange while Enterobacter agglutinate, Moellerella wisconsis were isolated from rhizosphere of timber. The silver particles bioremediation on polluted soil ranges were recorded within 5 days interval for 30 days. It was deduced that Enterobacter sp appeared to be the fastest on polluted soil followed by Citrobacter sp, Providencia sp, Serratia sp and Yersinia sp. Conclusion: Findings from this study revealed the effects of rhizosphere Gram negative bacteria in cleaning up crude oil polluted soil environment and the usefulness of silver particles in remediating hydrocarbon polluted soil.

Lectin activity of winter wheat seedlings affected by eyespot causal agent under the action of Bacillus subtilis 537/Б1 bacterial isolates

2018 ◽  
Vol 96 (10) ◽  
pp. 27-34
Author(s):  
M. Musiienko ◽  
L. Batsmanova ◽  
Ju. Pys'menna ◽  
T. Kondratiuk ◽  
N. Taran ◽  
...  
Keyword(s):  
Bacillus Subtilis ◽  
Winter Wheat ◽  
Causal Agent ◽  
Bacterial Isolates ◽  
Lectin Activity ◽  
Wheat Seedlings

scholarly journals Streptomycin-Induced Expression in Bacillus subtilis of YtnP, a Lactonase-Homologous Protein That Inhibits Development and Streptomycin Production in Streptomyces griseus

2011 ◽  
Vol 78 (2) ◽  
pp. 599-603 ◽  
Author(s):  
Johannes Schneider ◽  
Ana Yepes ◽  
Juan C. Garcia-Betancur ◽  
Isa Westedt ◽  
Benjamin Mielich ◽  
...  
Keyword(s):  
Bacillus Subtilis ◽  
Signaling Pathway ◽  
Streptomyces Griseus ◽  
Aerial Mycelium ◽  
Positive Bacterium ◽  
Gram Positive ◽  
Induced Expression ◽  
Content Type ◽  
Homologous Protein

ABSTRACTBacillus subtilisinduces expression of the geneytnPin the presence of the antimicrobial streptomycin, produced by the Gram-positive bacteriumStreptomyces griseus.ytnPencodes a lactonase-homologous protein that is able to inhibit the signaling pathway required for the streptomycin production and development of aerial mycelium inS. griseus.

The effect of filter material on bioaerosol collection of Bacillus subtilis spores used as a Bacillus anthracis simulant

2005 ◽  
Vol 7 (5) ◽  
pp. 475 ◽  
Author(s):  
Nancy Clark Burton ◽  
Atin Adhikari ◽  
Sergey A. Grinshpun ◽  
Richard Hornung ◽  
Tiina Reponen
Keyword(s):  
Bacillus Subtilis ◽  
Bacillus Anthracis ◽  
Filter Material

Effect of metal ions on lipopeptide secretion from Bacillus subtilis strain FJAT‐4: negative regulation by Ca 2+

2021 ◽  
Author(s):  
Meichun Chen ◽  
Meixia Zheng ◽  
Yanping Chen ◽  
Rongfeng Xiao ◽  
Xuefang Zheng ◽  
...  
Keyword(s):  
Bacillus Subtilis ◽  
Metal Ions ◽  
Negative Regulation ◽  
Subtilis Strain

scholarly journals Detection of pathogenic bacteria associated with earphones used by students of Stamford University Bangladesh

2020 ◽  
Vol 10 (1) ◽  
pp. 1-4
Author(s):  
Omor Ahmed Chowdhury ◽  
Md Raihan Ahmed ◽  
Md Raihan Dipu ◽  
Md Aftab Uddin
Keyword(s):  
Pathogenic Bacteria ◽  
Diffusion Method ◽  
Resistant Bacteria ◽  
Bacterial Isolates ◽  
Biochemical Tests ◽  
Disk Diffusion Method ◽  
Antibiotic Resistant ◽  
Potential Source ◽  
Different Types ◽  
Staphylococcus Spp

The use of earphones has increased in recent times throughout the world especially among the different level of students such as school, college or university who have a higher tendency of sharing these among them. Unlike airline headsets, headphones and stethoscope ear-pieces, ear phones are often shared by multiple users and can be a potential medium for transmission of pathogens, which can give rise to various ear related infections. The objective of this study was to detect the pathogenic bacteria from the ear-phones used by the students of Stamford University Bangladesh. A total of 16 ear-phone swabs were collected by sterile cotton swabs. The swabs were inoculated onto blood agar and incubated aerobically overnight at 37oC. Microscopic observation and standard biochemical tests were performed to confirm the identification of all the bacterial isolates. Six presumptively identified Staphylococcus spp. (38%) were tested against six different types of antibiotics following Kirby-Bauer disk diffusion method. Isolates were found to be 84% resistant against Cotrimoxazole and demonstrated 100% sensitivity to Vancomycin and Ciprorofloxacin. The findings of this study suggest the users to disinfect their respective ear phones and not to exchange them as they may act as a potential source to transfer pathogenic and antibiotic resistant bacteria among the ear phone users. Stamford Journal of Microbiology, Vol.10 (1) 2020: 1-4

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