Optical signature of erythrocytes by light scattering in microfluidic flows

Lab on a Chip ◽  
2015 ◽  
Vol 15 (16) ◽  
pp. 3278-3285 ◽  
Author(s):  
D. Dannhauser ◽  
D. Rossi ◽  
F. Causa ◽  
P. Memmolo ◽  
A. Finizio ◽  
...  
Keyword(s):  
Cell Migration ◽  
Light Scattering ◽  
Label Free ◽  
Optical Signature ◽  
Microfluidic Flows ◽  
Individual Erythrocytes ◽  
Migration Technique

Label-free analysis of individual erythrocytes by a camera-based light scattering approach coupled with a viscoelasticity-induced cell migration technique in microfluidic flows.

scholarly journals Characterization of Recombinant Adeno-Associated Viruses (rAAVs) for Gene Therapy Using Orthogonal Techniques

Pharmaceutics ◽  
2021 ◽  
Vol 13 (4) ◽  
pp. 586
Author(s):  
Liam Cole ◽  
Diogo Fernandes ◽  
Maryam T. Hussain ◽  
Michael Kaszuba ◽  
John Stenson ◽  
...  
Keyword(s):  
Gene Therapy ◽  
Light Scattering ◽  
Dynamic Light Scattering ◽  
Viral Vector ◽  
Structural Characteristics ◽  
Genetic Material ◽  
Label Free ◽  
Gene Therapies ◽  
Multiple Challenges

Viruses are increasingly used as vectors for delivery of genetic material for gene therapy and vaccine applications. Recombinant adeno-associated viruses (rAAVs) are a class of viral vector that is being investigated intensively in the development of gene therapies. To develop efficient rAAV therapies produced through controlled and economical manufacturing processes, multiple challenges need to be addressed starting from viral capsid design through identification of optimal process and formulation conditions to comprehensive quality control. Addressing these challenges requires fit-for-purpose analytics for extensive characterization of rAAV samples including measurements of capsid or particle titer, percentage of full rAAV particles, particle size, aggregate formation, thermal stability, genome release, and capsid charge, all of which may impact critical quality attributes of the final product. Importantly, there is a need for rapid analytical solutions not relying on the use of dedicated reagents and costly reference standards. In this study, we evaluate the capabilities of dynamic light scattering, multiangle dynamic light scattering, and SEC–MALS for analyses of rAAV5 samples in a broad range of viral concentrations (titers) at different levels of genome loading, sample heterogeneity, and sample conditions. The study shows that DLS and MADLS® can be used to determine the size of full and empty rAAV5 (27 ± 0.3 and 33 ± 0.4 nm, respectively). A linear range for rAAV5 size and titer determination with MADLS was established to be 4.4 × 1011–8.7 × 1013 cp/mL for the nominally full rAAV5 samples and 3.4 × 1011–7 × 1013 cp/mL for the nominally empty rAAV5 samples with 3–8% and 10–37% CV for the full and empty rAAV5 samples, respectively. The structural stability and viral load release were also inferred from a combination of DLS, SEC–MALS, and DSC. The structural characteristics of the rAAV5 start to change from 40 °C onward, with increasing aggregation observed. With this study, we explored and demonstrated the applicability and value of orthogonal and complementary label-free technologies for enhanced serotype-independent characterization of key properties and stability profiles of rAAV5 samples.

scholarly journals Label-Free Chromatin-DNA Imaging by Circular Polarized Light Scattering Scanning Microscopy

2019 ◽  
Vol 116 (3) ◽  
pp. 499a
Author(s):  
Aymeric Le Gratiet ◽  
Riccardo Marongiu ◽  
Luca Pesce ◽  
Michele Oneto ◽  
Paolo Bianchini ◽  
...  
Keyword(s):  
Light Scattering ◽  
Polarized Light ◽  
Scanning Microscopy ◽  
Label Free ◽  
Dna Imaging ◽  
Circular Polarized Light

Phosphodiesters quaternary ammonium nanoparticles as label-free light scattering probe for turn-off detection of tyrosine

2019 ◽  
Vol 208 ◽  
pp. 1-6 ◽  
Author(s):  
Da-Qian Feng ◽  
Guoliang Liu ◽  
Gangpo Ma ◽  
Zhe Nan ◽  
Wei Wang
Keyword(s):  
Light Scattering ◽  
Quaternary Ammonium ◽  
Label Free

scholarly journals Two‐Dimensional Light Scattering Anisotropy Cytometry for Label‐Free Classification of Ovarian Cancer Cells via Machine Learning

2019 ◽  
Vol 97 (1) ◽  
pp. 24-30
Author(s):  
Xuantao Su ◽  
Tao Yuan ◽  
Zhiwen Wang ◽  
Kun Song ◽  
Rongrong Li ◽  
...  
Keyword(s):  
Machine Learning ◽  
Ovarian Cancer ◽  
Light Scattering ◽  
Cancer Cells ◽  
Ovarian Cancer Cells ◽  
Two Dimensional ◽  
Label Free ◽  
Scattering Anisotropy ◽  
Free Classification

A highly sensitive label-free resonance light scattering assay of carcinoembryonic antigen based on immune complexes

2012 ◽  
Vol 747 ◽  
pp. 99-105 ◽  
Author(s):  
Zhanguang Chen ◽  
Yulong Lei ◽  
Zhisen Liang ◽  
Fengji Li ◽  
Lidong Liu ◽  
...  
Keyword(s):  
Light Scattering ◽  
Carcinoembryonic Antigen ◽  
Immune Complexes ◽  
Resonance Light Scattering ◽  
Label Free ◽  
Highly Sensitive

Elastic and inelastic light scattering spectroscopy and its possible use for label-free brain tumor typing

2017 ◽  
Vol 409 (28) ◽  
pp. 6613-6623 ◽  
Author(s):  
Edwin Ostertag ◽  
Mona Stefanakis ◽  
Karsten Rebner ◽  
Rudolf W. Kessler
Keyword(s):  
Brain Tumor ◽  
Light Scattering ◽  
Label Free ◽  
Inelastic Light Scattering ◽  
Light Scattering Spectroscopy

2D light scattering label-free cytometry using light-sheet illumination

2016 ◽  
Author(s):  
Meiai Lin ◽  
Xuantao Su
Keyword(s):  
Light Scattering ◽  
Light Sheet ◽  
Label Free

Label-free whole blood cell differentiation based on multiple frequency AC impedance and light scattering analysis in a micro flow cytometer

Lab on a Chip ◽  
2016 ◽  
Vol 16 (12) ◽  
pp. 2326-2338 ◽  
Author(s):  
Peter Simon ◽  
Marcin Frankowski ◽  
Nicole Bock ◽  
Jörg Neukammer
Keyword(s):  
Cell Differentiation ◽  
Light Scattering ◽  
Blood Cell ◽  
Whole Blood ◽  
Electrical Impedance ◽  
Label Free ◽  
Multiple Frequency ◽  
Flow Cytometric ◽  
Micro Flow ◽  
Flow Cytometric Cell

We developed a microfluidic sensor for label-free flow cytometric cell differentiation by combined multiple AC electrical impedance and light scattering analysis.

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