Quantification of proteins using enhanced etching of Ag coated Au nanorods by the Cu2+/bicinchoninic acid pair with improved sensitivity

Nanoscale ◽  
2016 ◽  
Vol 8 (2) ◽  
pp. 780-784 ◽  
Author(s):  
Wenqi Liu ◽  
Shuai Hou ◽  
Jiao Yan ◽  
Hui Zhang ◽  
Yinglu Ji ◽  
...  

We present an SPR detection method to quantify proteins by building up the relationship between the LSPR peak shift of Au@Ag nanorods and the protein amount via Cu2+/BCA pair bridged protein oxidation and Au@Ag etching.

Electronics ◽  
2021 ◽  
Vol 10 (7) ◽  
pp. 800
Author(s):  
Jongchan Park ◽  
Min-Hyun Kim ◽  
Dong-Geol Choi

Deep learning-based methods have achieved good performance in various recognition benchmarks mostly by utilizing single modalities. As different modalities contain complementary information to each other, multi-modal based methods are proposed to implicitly utilize them. In this paper, we propose a simple technique, called correspondence learning (CL), which explicitly learns the relationship among multiple modalities. The multiple modalities in the data samples are randomly mixed among different samples. If the modalities are from the same sample (not mixed), then they have positive correspondence, and vice versa. CL is an auxiliary task for the model to predict the correspondence among modalities. The model is expected to extract information from each modality to check correspondence and achieve better representations in multi-modal recognition tasks. In this work, we first validate the proposed method in various multi-modal benchmarks including CMU Multimodal Opinion-Level Sentiment Intensity (CMU-MOSI) and CMU Multimodal Opinion Sentiment and Emotion Intensity (CMU-MOSEI) sentiment analysis datasets. In addition, we propose a fraud detection method using the learned correspondence among modalities. To validate this additional usage, we collect a multi-modal dataset for fraud detection using real-world samples for reverse vending machines.


Molecules ◽  
2019 ◽  
Vol 24 (11) ◽  
pp. 2162 ◽  
Author(s):  
Chunhuan Jin ◽  
Zijun Wu ◽  
Lili Wang ◽  
Yoshikatsu Kanai ◽  
Xin He

Celastrol and triptolide, as the two main bio-activity ingredients in Tripterygium wilfordii, have wide anticancer pharmacological potency, as well as anti-inflammatory and immunosuppression effects. However, they have potential hepatotoxicity and underlying mechanisms of them-induced toxicity mediated by hepatic CYP450s have not been well delineated. In the present study, we accessed the toxic effects and possible mechanism of celastrol and triptolide on primary rat hepatocytes. Models of subdued/enhanced activity of CYP450 enzymes in primary rat hepatocytes were also constructed to evaluate the relationship between the two ingredients and CYP450s. LC-MS/MS was used to establish a detection method of the amount of triptolide in rat hepatocytes. As the results, cell viability, biochemical index, and mitochondrial membrane potential indicated that celastrol and triptolide had toxic potencies on hepatocytes. Moreover, the toxic effects were enhanced when the compounds combined with 1-aminobenzotriazole (enzyme inhibitor) while they were mitigated when combined with phenobarbital (an enzyme inducer). Meanwhile, celastrol could affect the amount of triptolide in the cell. We therefore put forward that increase of triptolide in the cell might be one of the main causes of hepatotoxicity caused by Tripterygium wilfordii.


Sensors ◽  
2020 ◽  
Vol 20 (20) ◽  
pp. 5895
Author(s):  
Jiansu Pu ◽  
Jingwen Zhang ◽  
Hui Shao ◽  
Tingting Zhang ◽  
Yunbo Rao

The development of the Internet has made social communication increasingly important for maintaining relationships between people. However, advertising and fraud are also growing incredibly fast and seriously affect our daily life, e.g., leading to money and time losses, trash information, and privacy problems. Therefore, it is very important to detect anomalies in social networks. However, existing anomaly detection methods cannot guarantee the correct rate. Besides, due to the lack of labeled data, we also cannot use the detection results directly. In other words, we still need human analysts in the loop to provide enough judgment for decision making. To help experts analyze and explore the results of anomaly detection in social networks more objectively and effectively, we propose a novel visualization system, egoDetect, which can detect the anomalies in social communication networks efficiently. Based on the unsupervised anomaly detection method, the system can detect the anomaly without training and get the overview quickly. Then we explore an ego’s topology and the relationship between egos and alters by designing a novel glyph based on the egocentric network. Besides, it also provides rich interactions for experts to quickly navigate to the interested users for further exploration. We use an actual call dataset provided by an operator to evaluate our system. The result proves that our proposed system is effective in the anomaly detection of social networks.


2012 ◽  
Vol 253-255 ◽  
pp. 2130-2134 ◽  
Author(s):  
Li Bei ◽  
Zhang Di ◽  
Lu Lu Du

By theoretical analysis and experiment, this article studied the rebound voltage of lead-acid battery when off-lined. By considering elements as discharge rate, depth of discharge, and environment temperature and so on, we experimented and found the relationship between the rebound voltage and the state of charge (SOC) of battery, which provided reliable basis to estimate the SOC of battery by rebound voltage.


2013 ◽  
Vol 706-708 ◽  
pp. 575-578
Author(s):  
Jia Le Song ◽  
Chang Yong Ye ◽  
Bai Lin Wang ◽  
Zhi Mi Zhou ◽  
Yan Xiao ◽  
...  

The relationship between the SBS modifier types and asphalt components on the effect of modified asphalt properties was discussed in this paper. Higher block ratio, higher molecular weight and star-like structure of SBS can improve modified asphalt high temperature properties. Colloid content decrease leading the processing easier, result stability decline. Our research group invented a chemical titration combined with fluorescence microscopic analysis detection method monitoring SBS modifier dosage. This method applied in highway construction site, the effect is significant.


Energies ◽  
2019 ◽  
Vol 12 (2) ◽  
pp. 323 ◽  
Author(s):  
Qiwei Lu ◽  
Zeyu Ye ◽  
Yilei Zhang ◽  
Tao Wang ◽  
Zhixuan Gao

Owing to the shortcomings of existing series arc fault detection methods, based on a summary of arc volt–ampere characteristics, the change rule of the line current and the relationship between the voltage and current are deeply analyzed and theoretically explained under different loads when series arc faults occur. A series arc fault detection method is proposed, and the software flowchart and principles of the applied hardware implementation are given. Finally, a prototype of an arc fault detection device (AFDD) with a rated voltage of 220 V and a rated current of 40 A is developed. The prototype was tested according to experimental methods provided by the Chinese national standard, GB/T 31143-2014. The experimental results show that the proposed detection method is simple and practical, and can be implemented using a low-cost microprocessor. The proposed method will provide good theoretical guidance in promoting the research and development of an AFDD.


2009 ◽  
Vol 5 (S264) ◽  
pp. 105-108
Author(s):  
Ganghua Lin

AbstractThe methods of automatic solar active phenomenon or event detection have been researched and explored by people for many years, which have gone into actual services. The paper analyzes the relationship between these methods of automatic detection and the forecast or alert, using the solar short-term proton events predictions as an example. Using automatic method to conduct forecast or alert is under thinking.


2020 ◽  
Vol 404 ◽  
pp. 61-67
Author(s):  
Bernd Breidenstein ◽  
Nils Vogel ◽  
Marcel Dietrich ◽  
Harald Behrens ◽  
Jon M. Andersson

Residual stress measurements directly in the coated cutting edge are not possible with X-ray diffraction (XRD) due to the diameter of the X-ray beam. On the other hand, Raman microscopy enables measurements on the micrometer scale. Parameter variations in the PVD process were used to provide different residual stress states in (Al,Ti)N coatings on carbide cutting tools. They were examined by XRD in regions that can be reliably measured. The same area was then examined by Raman microscopy to determine the relationship of Raman peaks to the residual stress. Local high-resolution Raman measurements were then taken at the cutting edge and further influences on the Raman peak position besides residual stresses were excluded. In order to analyze the relationship between Raman peak shift and residual stress state, measurements were performed during a bending load. Finally, an outlook on further investigations is given.


2014 ◽  
Vol 2014 ◽  
pp. 1-10 ◽  
Author(s):  
Guangwei Zhu ◽  
Guijun Zhang ◽  
Meng Wang ◽  
Jingjuan Wang ◽  
Weixin Zeng ◽  
...  

A simple and sensitive HPLC-DAD detection method was established for the simultaneous determination of nine compounds including oxypaeoniflorin, albiflorin, paeoniflorin, benzoylpaeoniflorin, glycyrrhizic acid, liquiritin, isoliquiritin, liquiritigenin, and isoliquiritigenin in the Traditional Chinese Medicinal Prescription Shaoyao-Gancao-Tang (SGT) and we analyze the relationship between therapeutical effect and compatibility of medicines by using an Agilent extend-C18 column at a flow rate of 1 mL/min. The column temperature was maintained at 30°C and the detection wavelength was set at 230 nm for oxypaeoniflorin, albiflorin, paeoniflorin, benzoylpaeoniflorin, and glycyrrhizic acid; 276 nm for liquiritin and liquiritigenin; 360 nm for isoliquiritin and isoliquiritigenin. The total contents of the nine compounds in SGT varied from 4.65 to 20.06 mg/mL. The results of this study showed that the content of chemical compounds of Traditional Chinese Medicinal Prescription is mainly influenced by the dosage and compatibility of medicines and the therapeutical effect of Traditional Chinese Medicinal prescription is mainly influenced by the dosage and compatibility of medicines. The method could be suitable for quality control of SGT with bioactive multicompounds.


2015 ◽  
Vol 2015 ◽  
pp. 1-9 ◽  
Author(s):  
Huiyuan Gu ◽  
Lin Li ◽  
Min Gu ◽  
Guoxin Zhang

Background. Some studies have shown the possible involvement ofHelicobacter pylori(H. pylori) infection in chronic urticaria, but the relationship remains controversial. The aim of this meta-analysis was to quantitatively assess the association betweenH. pyloriinfection and chronic urticaria.Methods. Observational studies comparing the prevalence ofH. pyloriinfection in patients with chronic urticaria and control subjects were identified through a systematic search in MEDLINE and EMBASE up to July 2014.H. pyloriinfection was confirmed by serological or nonserological tests. For subgroup analyses, studies were separated by region, publication year, andH. pyloridetection method to screen the potential factors resulting in heterogeneity.Results. 16 studies involving 965 CU cases and 1235 controls were included. Overall, the prevalence ofH. pyloriinfection was higher in urticarial patients than in controls (OR = 1.66; 95% CI: 1.12–2.45;P=0.01). This result persisted in subanalysis of nine high-quality studies (OR = 1.36; 95% CI: 1.03–1.80;P=0.03). Subgroup analysis showed that detection method ofH. pyloriis also a potential influential factor for the overall results.Conclusions. Our present meta-analysis suggests thatH. pyloriinfection is significantly, though weakly, associated with an increased risk of chronic urticaria.


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