scholarly journals Fabrication of poly(ε-caprolactone) tissue engineering scaffolds with fibrillated and interconnected pores utilizing microcellular injection molding and polymer leaching

RSC Advances ◽  
2017 ◽  
Vol 7 (69) ◽  
pp. 43432-43444 ◽  
Author(s):  
An Huang ◽  
Yongchao Jiang ◽  
Brett Napiwocki ◽  
Haoyang Mi ◽  
Xiangfang Peng ◽  
...  
Keyword(s):  
Tissue Engineering ◽  
Injection Molding ◽  
Three Dimensional ◽  
Tissue Engineering Scaffolds ◽  
Microcellular Injection Molding ◽  
Interconnected Pores ◽  
Porous Poly

Three-dimensional fibrillated interconnected porous poly(ε-caprolactone) scaffolds were prepared by microcellular injection molding and polymer leaching.

HYDROXYAPATITE, ALGINATE, AND CHITOSAN FOR BONE SCAFFOLDS: SPECTROSCOPY STUDY

2016 ◽  
Vol 19 (2) ◽  
pp. 93-100
Author(s):  
Lalita El Milla
Keyword(s):  
Tissue Engineering ◽  
Bone Tissue Engineering ◽  
Bone Tissue ◽  
Functional Groups ◽  
Bone Growth ◽  
Three Dimensional ◽  
Dimensional Structure ◽  
Tissue Engineering Scaffolds ◽  
Hydroxyapatite Powder ◽  
Materials Used

Scaffolds is three dimensional structure that serves as a framework for bone growth. Natural materials are often used in synthesis of bone tissue engineering scaffolds with respect to compliance with the content of the human body. Among the materials used to make scafffold was hydroxyapatite, alginate and chitosan. Hydroxyapatite powder obtained by mixing phosphoric acid and calcium hydroxide, alginate powders extracted from brown algae and chitosan powder acetylated from crab. The purpose of this study was to examine the functional groups of hydroxyapatite, alginate and chitosan. The method used in this study was laboratory experimental using Fourier Transform Infrared (FTIR) spectroscopy for hydroxyapatite, alginate and chitosan powders. The results indicated the presence of functional groups PO43-, O-H and CO32- in hydroxyapatite. In alginate there were O-H, C=O, COOH and C-O-C functional groups, whereas in chitosan there were O-H, N-H, C=O, C-N, and C-O-C. It was concluded that the third material containing functional groups as found in humans that correspond to the scaffolds material in bone tissue engineering.

scholarly journals Three-Dimensional Microfluidic Tissue-Engineering Scaffolds Using a Flexible Biodegradable Polymer

2006 ◽  
Vol 18 (2) ◽  
pp. 165-169 ◽  
Author(s):  
C. J. Bettinger ◽  
E. J. Weinberg ◽  
K. M. Kulig ◽  
J. P. Vacanti ◽  
Y. Wang ◽  
...  
Keyword(s):  
Tissue Engineering ◽  
Biodegradable Polymer ◽  
Three Dimensional ◽  
Tissue Engineering Scaffolds

Mineralized Nanofibers for Bone Tissue Engineering

2018 ◽  
pp. 461-475 ◽  
Author(s):  
Ozan Karaman
Keyword(s):  
Tissue Engineering ◽  
Bone Tissue Engineering ◽  
Bone Regeneration ◽  
Bone Tissue ◽  
Three Dimensional ◽  
Bone Grafts ◽  
Tissue Engineering Scaffolds ◽  
Promising Alternative ◽  
Biomimetic Scaffolds

The limitation of orthopedic fractures and large bone defects treatments has brought the focus on fabricating bone grafts that could enhance ostegenesis and vascularization in-vitro. Developing biomimetic materials such as mineralized nanofibers that can provide three-dimensional templates of the natural bone extracellular-matrix is one of the most promising alternative for bone regeneration. Understanding the interactions between the structure of the scaffolds and cells and therefore the control cellular pathways are critical for developing functional bone grafts. In order to enhance bone regeneration, the engineered scaffold needs to mimic the characteristics of composite bone ECM. This chapter reviews the fabrication of and fabrication techniques for fabricating biomimetic bone tissue engineering scaffolds. In addition, the chapter covers design criteria for developing the scaffolds and examples of enhanced osteogenic differentiation outcomes by fabricating biomimetic scaffolds.

The Fabrication of Tissue Engineering Scaffolds by Inkjet Printing Technology

2018 ◽  
Vol 934 ◽  
pp. 129-133 ◽  
Author(s):  
Chao Fan Lv ◽  
Li Ya Zhu ◽  
Jian Ping Shi ◽  
Zong An Li ◽  
Wen Lai Tang ◽  
...  
Keyword(s):  
Tissue Engineering ◽  
Sodium Alginate ◽  
Inkjet Printing ◽  
Three Dimensional ◽  
3D Bioprinting ◽  
Tissue Engineering Scaffolds ◽  
Printing Technology ◽  
3D Printed ◽  
Inkjet Printing Technology ◽  
Alginate Scaffold

Three-dimensional (3D) printing has been playing an important role in diverse areas in medicine. In order to promote the development of tissue engineering, this study attempts to fabricate tissue engineering scaffolds using the inkjet printing technology. Sodium alginate, exhibiting similar properties to the native human extracellular matrix (ECM), was used as bioink. The jetted fluid of sodium alginate would be gelatinized when printed into the calcium chloride solution. The characteristics of the 3D-printed sodium alginate scaffold were systematically measured and analyzed. The results show that, the pore size, porosity and degradation property of these scaffolds could be well controlled. This study indicates the capability of 3D bioprinting technology for preparing tissue engineering scaffolds.

Microcellular Injection Molding of Thermoplastic Polyurethane (TPU) Scaffolds Using Carbon Dioxide and Water as Co-Blowing Agents

2013 ◽  
Author(s):  
Hao-Yang Mi ◽  
Xin Jing ◽  
Lih-Sheng Turng ◽  
Xiang-Fang Peng
Keyword(s):  
Mechanical Properties ◽  
Tissue Engineering ◽  
Thermoplastic Polyurethane ◽  
Dynamic Mechanical Properties ◽  
Skin Layer ◽  
Tissue Engineering Scaffolds ◽  
Blowing Agents ◽  
Microcellular Injection Molding ◽  
Noticeable Reduction ◽  
Free Transport

In this study, a novel microcellular injection foaming method employing supercritical CO2 (scCO2) and water as co-blowing agents was developed to produce thermoplastic polyurethane (TPU) tissue engineering scaffolds with a uniform porous structure and no solid skin layer. Various characterization techniques were applied to investigate the cell morphology, crystallization behavior, and static and dynamic mechanical properties of solid molded samples, foamed samples using CO2 or water as a single blowing agent, and foamed samples using both CO2 and water as co-blowing agents. Compared with CO2 foamed scaffolds, scaffolds produced by the co-blowing method exhibit much more uniform cell morphologies without a noticeable reduction in mechanical properties. Moreover, these TPU scaffolds have almost no skin layer, which permits free transport of nutrients and waste throughout the samples, which is highly desirable in tissue engineering. The effect of these blowing agents on the shear viscosity of various samples is also reported.

Hydroxyapatite/Biopolymers Composite Scaffolds for Bone Tissue Engineering

2011 ◽  
Vol 493-494 ◽  
pp. 826-831
Author(s):  
A.C.B.M. Fook ◽  
Thiago Bizerra Fideles ◽  
R.C. Barbosa ◽  
G.T.F.S. Furtado ◽  
G.Y.H. Sampaio ◽  
...  
Keyword(s):  
Tissue Engineering ◽  
Bone Tissue Engineering ◽  
Bone Tissue ◽  
Freeze Drying ◽  
Three Dimensional ◽  
Porous Scaffolds ◽  
X Ray Diffraction ◽  
X Ray ◽  
Interconnected Pores

The application of a hybrid composite consisting of biopolymer and calcium phosphate, similar morphology and properties of natural bone, may be a way to solve the problem of the fragility of ceramics without reducing its mechanical properties, retaining the properties of biocompatibility and high bioactivity. This work aims at the preparation and characterization of three-dimensional scaffolds composite HA / biopolymers (chitosan and gelatin). The freeze-drying technique was employed in this study to obtain these frameworks and partial results showed the effectiveness of this method. This involved the study of structural, chemical and morphological frameworks, in order to direct the research suggested the application. The X Ray Diffraction (XRD) and infrared spectroscopy and Fourier transform (FTIR) results confirmed the formation of hydroxyapatite (HA) phase and the presence of characteristic bands of HA and biopolymers in all compositions. The microstructure of the scaffolds study conducted by Scanning Electron Microscopy (SEM) revealed the formation of longitudinally oriented microchannels with interconnected pores. In all compositions the porous scaffolds showed varying sizes and mostly larger than 100μm, and is therefore considered materials with potential for application in bone tissue engineering.

scholarly journals Hybrid Tissue Engineering Scaffolds by Combination of Three-Dimensional Printing and Cell Photoencapsulation

2015 ◽  
Vol 6 (2) ◽  
Author(s):  
Marica Markovic ◽  
Jasper Van Hoorick ◽  
Katja Hölzl ◽  
Maximilian Tromayer ◽  
Peter Gruber ◽  
...  
Keyword(s):  
Tissue Engineering ◽  
Fused Deposition Modeling ◽  
Structural Parameters ◽  
Three Dimensional ◽  
Poly Lactic Acid ◽  
Tissue Engineering Scaffolds ◽  
Ecm Remodeling ◽  
Initial Cell ◽  
Fused Deposition ◽  
Hydrogel Matrix

Three-dimensional (3D) printing offers versatile possibilities for adapting the structural parameters of tissue engineering scaffolds. However, it is also essential to develop procedures allowing efficient cell seeding independent of scaffold geometry and pore size. The aim of this study was to establish a method for seeding the scaffolds using photopolymerizable cell-laden hydrogels. The latter facilitates convenient preparation, and handling of cell suspension, while distributing the hydrogel precursor throughout the pores, before it is cross-linked with light. In addition, encapsulation of living cells within hydrogels can produce constructs with high initial cell loading and intimate cell-matrix contact, similar to that of the natural extra-cellular matrix (ECM). Three dimensional scaffolds were produced from poly(lactic) acid (PLA) by means of fused deposition modeling. A solution of methacrylamide-modified gelatin (Gel-MOD) in cell culture medium containing photoinitiator Li-TPO-L was used as a hydrogel precursor. Being an enzymatically degradable derivative of natural collagen, gelatin-based matrices are biomimetic and potentially support the process of cell-induced remodeling. Preosteoblast cells MC3T3-E1 at a density of 10 × 106 cells per 1 mL were used for testing the seeding procedure and cell proliferation studies. Obtained results indicate that produced constructs support cell survival and proliferation over extended duration of our experiment. The established two-step approach for scaffold seeding with the cells is simple, rapid, and is shown to be highly reproducible. Furthermore, it enables precise control of the initial cell density, while yielding their uniform distribution throughout the scaffold. Such hybrid tissue engineering constructs merge the advantages of rigid 3D printed constructs with the soft hydrogel matrix, potentially mimicking the process of ECM remodeling.

scholarly journals A Nanofiber Mat With Dual Bioactive Components and a Biomimetic Matrix Structure for Improving Osteogenesis Effect

2021 ◽  
Vol 9 ◽  
Author(s):  
Yadi Han ◽  
Xiaofeng Shen ◽  
Sihao Chen ◽  
Xiuhui Wang ◽  
Juan Du ◽  
...  
Keyword(s):  
Tissue Engineering ◽  
Lactic Acid ◽  
Bone Repair ◽  
Three Dimensional ◽  
Dimensional Structure ◽  
Bioactive Components ◽  
Tissue Engineering Scaffolds ◽  
Cell Growth And Migration ◽  
Nanofiber Mats

The challenge of effectively regenerating bone tissue through tissue engineering technology is that most tissue engineering scaffolds cannot imitate the three-dimensional structure and function of the natural extracellular matrix. Herein, we have prepared the poly(L-lactic acid)–based dual bioactive component reinforced nanofiber mats which were named as poly(L-lactic acid)/bovine serum albumin/nanohydroxyapatite (PLLA/BSA/nHAp) with dual bioactive components by combining homogeneous blending and electrospinning technology. The results showed that these nanofiber mats had sufficient mechanical properties and a porous structure suitable for cell growth and migration. Furthermore, the results of cell experiments in vitro showed that PLLA/BSA/nHAp composite nanofiber mat could preferably stimulate the proliferation of mouse osteoblastic cells (MC3T3 cells) compared with pure PLLA nanofiber mats. Based on these results, the scaffolds developed in this study are considered to have a great potential to be adhibited as bone repair materials.

Sign in / Sign up

Export Citation Format

Share Document