Sensitive and selective monitoring of the DNA damage-induced intracellular p21 protein and unraveling the role of the p21 protein in DNA repair and cell apoptosis by surface plasmon resonance

The Analyst ◽  
2020 ◽  
Vol 145 (10) ◽  
pp. 3697-3704 ◽  
Author(s):  
Yuhan He ◽  
Zixiao Wang ◽  
Yuqing Hu ◽  
Xinyao Yi ◽  
Ling Wu ◽  
...  
Keyword(s):  
Dna Damage ◽  
Dna Repair ◽  
Surface Plasmon Resonance ◽  
Surface Plasmon ◽  
Plasmon Resonance ◽  
Cell Apoptosis ◽  
P21 Protein ◽  
Selective Monitoring

Sensitive and selective monitoring of DNA damage-induced intracellular p21 protein is proposed using surface plasmon resonance. The method serves as a viable means for unraveling the role of p21 protein in DNA repair and cell apoptosis.

scholarly journals Kajian Pengaruh Material Graphene pada kinerja Biosensor Berbasis Surface Plasmon Resonance (SPR) pada Deteksi Makanan Halal sebagai Pendukung Halal Research Center UIN Sunan Kalijaga Yogyakarta

2017 ◽  
Vol 7 (1) ◽  
pp. 1
Author(s):  
Wida Yanti ◽  
Asih Melati
Keyword(s):  
Surface Plasmon Resonance ◽  
Surface Plasmon ◽  
Plasmon Resonance ◽  
Analytical Calculation ◽  
Detection Sensitivity ◽  
Research Center ◽  
Attenuated Total Reflectance ◽  
Muslim Community ◽  
Spr Biosensor

<p><br />Halal foods and medicines are an absolute daily needs for the Muslim community in Indonesia. Therefore the authority institutions in indonesian goverment should ensure the availability of this. It is of course inseparable from the role of higher education through the development of its technology to develop halal detection of foods and drugs. This study is an effort to contribute to the Halal Research Center of UIN Sunan Kalijaga Yogyakarta through the biosensor development in halal detection foods and medicines based on biosensor SPR. This device using graphene materials to improve the detection sensitivity of pork gelatin material that is likely contained in foodstuffs and medicine. From analytical calculation and computation, enhancement of the SPR biosensor performance by involvement graphene it was shown through the ATR (Attenuated Total Reflectance) reflective curve. The result of this results was found the enhancement of the sensitivity 2,86 %.</p><p>Keyword: Surface Plasmon Resonance (SPR), Porcine Gelatin, Graphene, ATR</p>

scholarly journals Modified Drude model for small gold nanoparticles surface plasmon resonance based on the role of classical confinement

2020 ◽  
Vol 10 (1) ◽  
Author(s):  
Asef Kheirandish ◽  
Nasser Sepehri Javan ◽  
Hosein Mohammadzadeh
Keyword(s):  
Gold Nanoparticles ◽  
Surface Plasmon Resonance ◽  
Surface Plasmon ◽  
Plasmon Resonance ◽  
Drude Model

Role of the mobility of antigen binding site in high affinity antibody elucidated by surface plasmon resonance

2016 ◽  
Vol 161 (1) ◽  
pp. 37-43 ◽  
Author(s):  
Natsuki Fukuda ◽  
Yoshiaki Suwa ◽  
Makiyo Uchida ◽  
Yoshihiro Kobashigawa ◽  
Hideshi Yokoyama ◽  
...  
Keyword(s):  
Surface Plasmon Resonance ◽  
Binding Site ◽  
Surface Plasmon ◽  
Plasmon Resonance ◽  
Antigen Binding ◽  
Antigen Binding Site ◽  
High Affinity

Emerging role of surface plasmon resonance in fragment-based drug discovery

2011 ◽  
Vol 3 (14) ◽  
pp. 1809-1820 ◽  
Author(s):  
Iva Navratilova ◽  
Andrew L Hopkins
Keyword(s):  
Surface Plasmon Resonance ◽  
Drug Discovery ◽  
Surface Plasmon ◽  
Plasmon Resonance ◽  
Fragment Based Drug Discovery

Non-spherical gold nanoparticles mediated surface plasmon resonance in Er3+ doped zinc–sodium tellurite glasses: Role of heat treatment

2014 ◽  
Vol 149 ◽  
pp. 138-143 ◽  
Author(s):  
Asmahani Awang ◽  
S.K. Ghoshal ◽  
M.R. Sahar ◽  
R. Arifin ◽  
Fakhra Nawaz
Keyword(s):  
Heat Treatment ◽  
Gold Nanoparticles ◽  
Surface Plasmon Resonance ◽  
Surface Plasmon ◽  
Plasmon Resonance ◽  
Tellurite Glasses ◽  
Sodium Tellurite ◽  
Spherical Gold Nanoparticles

scholarly journals Molecular flexibility of DNA as a key determinant of RAD51 recruitment

2018 ◽  
Author(s):  
Federico Paoletti ◽  
Afaf El-Sagheer ◽  
Jun Allard ◽  
Tom Brown ◽  
Omer Dushek ◽  
...  
Keyword(s):  
Dna Damage ◽  
Surface Plasmon Resonance ◽  
Homologous Recombination ◽  
Surface Plasmon ◽  
Plasmon Resonance ◽  
Genome Stability ◽  
General Framework ◽  
Binding Kinetics ◽  
Double Stranded Dna ◽  
Conceptual Question

AbstractThe timely activation of homologous recombination is essential for the maintenance of genome stability, in which the RAD51 recombinase plays a central role. Biochemically, human RAD51 polymerises faster on single-stranded DNA (ssDNA) compared to double-stranded DNA (dsDNA), raising a key conceptual question: how does it discriminate between them? In this study, we tackled this problem by systematically assessing RAD51 binding kinetics on ssDNA and dsDNA differing in length and flexibility using surface plasmon resonance. By fitting detailed polymerisation models informed by our experimental datasets, we show that RAD51 is a mechano-sensor that exhibits a larger polymerisation rate constant on flexible ssDNA compared to rigid ssDNA or dsDNA. This model presents a new general framework suggesting that the flexibility of DNA, which may increase locally as a result of DNA damage, plays an important role in rapidly recruiting repair factors that multimerise at sites of DNA damage.

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