A novel method for simultaneously screening superoxide anion scavengers and xanthine oxidase inhibitors using hydroethidine as a fluorescent probe coupled with high-performance liquid chromatography-mass spectrometry

2020 ◽  
Vol 12 (3) ◽  
pp. 255-263 ◽  
Author(s):  
Hongxia Li ◽  
Wen Zhang ◽  
Yu Fu ◽  
Ping Li ◽  
Wenhua Liu ◽  
...  
Keyword(s):  
Mass Spectrometry ◽  
Reactive Oxygen Species ◽  
Superoxide Anion ◽  
High Performance ◽  
Cellular Damage ◽  
Oxygen Species ◽  
Liquid Chromatography Mass Spectrometry ◽  
Xanthine Oxidase Inhibitors ◽  
Novel Method ◽  
Excess Reactive Oxygen Species

Excess reactive oxygen species can cause cellular damage, and are involved in many pathological processes such as inflammation, atherosclerosis and cancer.

scholarly journals Oxidative stress in pregnancy and reproduction

2016 ◽  
Vol 9 (3) ◽  
pp. 113-116 ◽  
Author(s):  
Kate Duhig ◽  
Lucy C Chappell ◽  
Andrew H Shennan
Keyword(s):  
Oxidative Stress ◽  
Reactive Oxygen Species ◽  
Preterm Labour ◽  
Membrane Integrity ◽  
Cellular Membrane ◽  
Cellular Damage ◽  
Oxygen Species ◽  
Overwhelming Evidence ◽  
Excess Reactive Oxygen Species ◽  
In Pregnancy

Oxidative stress is implicated in the pathophysiology of many reproductive complications including infertility, miscarriage, pre-eclampsia, fetal growth restriction and preterm labour. The presence of excess reactive oxygen species can lead to cellular damage of deoxyribonucleic acids, lipids and proteins. Antioxidants protect cells from peroxidation reactions, limiting cellular damage and helping to maintain cellular membrane integrity. There is overwhelming evidence for oxidative stress causing harm in reproduction. However, there is sparse evidence that supplementation with commonly used antioxidants (mostly vitamins C and E) makes any difference in overcoming oxidative stress or reversing disease processes. There may be potential for antioxidant therapy to ameliorate or prevent disease, but this requires a thorough understanding of the mechanism of action and specificity of currently used antioxidants.

scholarly journals A Novel Thiosemicarbazide-Based Fluorescent Chemosensor for Hypochlorite in Near-Perfect Aqueous Solution and Zebrafish

Chemosensors ◽  
2021 ◽  
Vol 9 (4) ◽  
pp. 65
Author(s):  
Minji Lee ◽  
Donghwan Choe ◽  
Soyoung Park ◽  
Hyeongjin Kim ◽  
Soomin Jeong ◽  
...  
Keyword(s):  
Mass Spectrometry ◽  
Reactive Oxygen Species ◽  
Aqueous Solution ◽  
Fluorescence Quenching ◽  
Limit Of Detection ◽  
Fluorescent Sensor ◽  
Ionization Mass ◽  
Oxygen Species ◽  
Marked Selectivity ◽  
Uv Visible

A novel thiosemicarbazide-based fluorescent sensor (AFC) was developed. It was successfully applied to detect hypochlorite (ClO−) with fluorescence quenching in bis-tris buffer. The limit of detection of AFC for ClO− was analyzed to be 58.7 μM. Importantly, AFC could be employed as an efficient and practical fluorescent sensor for ClO− in water sample and zebrafish. Moreover, AFC showed a marked selectivity to ClO− over varied competitive analytes with reactive oxygen species. The detection process of AFC to ClO− was illustrated by UV–visible and fluorescent spectroscopy and electrospray ionization–mass spectrometry (ESI–MS).

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