A chiral signal-amplified sensor for enantioselective discrimination of amino acids based on charge transfer-induced SERS

2019 ◽  
Vol 55 (65) ◽  
pp. 9697-9700 ◽  
Author(s):  
Yue Wang ◽  
Jing Liu ◽  
Xueqi Zhao ◽  
Chunguang Yang ◽  
Yukihiro Ozaki ◽  
...  
Keyword(s):  
Amino Acids ◽  
Charge Transfer ◽  
Discrimination Performance ◽  
High Sensitivity ◽  
Enantioselective Sensor

An ultra-high sensitivity enantioselective sensor with excellent discrimination performance for trace amino acids by using charge transfer-induced SERS.

scholarly journals Identification of novel translated small ORFs in Escherichia coli using complementary ribosome profiling approaches

2021 ◽  
Author(s):  
Anne Stringer ◽  
Carol Smith ◽  
Kyle Mangano ◽  
Joseph T. Wade
Keyword(s):  
Escherichia Coli ◽  
Amino Acids ◽  
High Sensitivity ◽  
Purifying Selection ◽  
Ribosome Profiling ◽  
Small Subset ◽  
Stop Codons ◽  
Small Proteins ◽  
Domains Of Life ◽  
Short Orfs

Small proteins of <51 amino acids are abundant across all domains of life but are often overlooked because their small size makes them difficult to predict computationally, and they are refractory to standard proteomic approaches. Ribosome profiling has been used to infer the existence of small proteins by detecting the translation of the corresponding open reading frames (ORFs). Detection of translated short ORFs by ribosome profiling can be improved by treating cells with drugs that stall ribosomes at specific codons. Here, we combine the analysis of ribosome profiling data for Escherichia coli cells treated with antibiotics that stall ribosomes at either start or stop codons. Thus, we identify ribosome-occupied start and stop codons with high sensitivity for ∼400 novel putative ORFs. The newly discovered ORFs are mostly short, with 365 encoding proteins of <51 amino acids. We validate translation of several selected short ORFs, and show that many likely encode unstable proteins. Moreover, we present evidence that most of the newly identified short ORFs are not under purifying selection, suggesting they do not impact cell fitness, although a small subset have the hallmarks of functional ORFs. IMPORTANCE Small proteins of <51 amino acids are abundant across all domains of life but are often overlooked because their small size makes them difficult to predict computationally, and they are refractory to standard proteomic approaches. Recent studies have discovered small proteins by mapping the location of translating ribosomes on RNA using a technique known as ribosome profiling. Discovery of translated sORFs using ribosome profiling can be improved by treating cells with drugs that trap initiating ribosomes. Here, we show that combining these data with equivalent data for cells treated with a drug that stalls terminating ribosomes facilitates the discovery of small proteins. We use this approach to discover 365 putative genes that encode small proteins in Escherichia coli .

A correlation of signed charge-transfer cotton effects with absolute configuration of α-amino acids in copper (II) complexes

1967 ◽  
Vol 8 (37) ◽  
pp. 3607-3611 ◽  
Author(s):  
K.M. Wellman ◽  
S. Bogdansky ◽  
W. Mungall ◽  
T.G. Mecca ◽  
C.R. Hare
Keyword(s):  
Amino Acids ◽  
Charge Transfer ◽  
Absolute Configuration ◽  
Cotton Effects

Electrochemical recognition of alkylimidazolium-mediated ultrafast charge transfer on graphene surfaces

2018 ◽  
Vol 54 (6) ◽  
pp. 666-669
Author(s):  
Hucheng Zhang ◽  
Mengmeng Wang ◽  
Jing Zhao ◽  
Shitao Han ◽  
Haixia Cheng ◽  
...  
Keyword(s):  
Charge Transfer ◽  
Electrochemical Method ◽  
Active Sites ◽  
High Sensitivity ◽  
Metal Free ◽  
Sensitivity And Selectivity ◽  
Donor Acceptor ◽  
Ultrafast Charge Transfer ◽  
Electrochemical Recognition

The charge transfer and active sites of metal-free imidazolium-based composites were unveiled by an electrochemical method with high sensitivity and selectivity due to the specific donor–acceptor coupling of imidazolium with NO2−.

The Synthesis of a Cubane-Substituted Dipeptide

2012 ◽  
Vol 65 (6) ◽  
pp. 690 ◽  
Author(s):  
Quentin I. Churches ◽  
Roger J. Mulder ◽  
Jonathan M. White ◽  
John Tsanaktsidis ◽  
Peter J. Duggan
Keyword(s):  
Amino Acids ◽  
Amino Acid ◽  
High Sensitivity ◽  
Pure Form ◽  
Bioactive Molecules ◽  
Side Chains ◽  
Cyclic Hydrocarbon ◽  
Wide Range ◽  
Effective Synthesis ◽  
Dipeptide Derivative

Amino acids and peptides bearing cyclic hydrocarbon side-chains are of interest in the development of a wide range of bioactive molecules. The preparation of an amino acid and a dipeptide derivative bearing an unfunctionalised cubane substituent is described. Attempts to prepare a cubylalanine derivative via the corresponding dehydroalanine were unsuccessful due to the high sensitivity of this vinyl cubane compound. Conversely, the addition of cubyllithium to a (RS)-glyoxylate sulfinimine led to an effective synthesis of a cubylglycine derivative and a cubane-substituted dipeptide in diastereomerically pure form.

Fluorinated aminoanthranilamides: non-native amino acids for bringing proteomic approaches to charge-transfer systems

2017 ◽  
Vol 19 (11) ◽  
pp. 7871-7876 ◽  
Author(s):  
Jillian M. Larsen-Clinton ◽  
Eli M. Espinoza ◽  
Maximillian F. Mayther ◽  
John Clark ◽  
Christina Tao ◽  
...  
Keyword(s):  
Amino Acids ◽  
Charge Transfer ◽  
Building Blocks ◽  
Selective Substitution

A highly selective substitution leads to a set of unique non-native amino acids that can serve as building blocks for molecular electrets.

The ligand mediated pseudohypersensitivity of the 3H4 → 3P2, 3H4 → 3P1, 3H4 → 3P0, and 3H4 → 1D2 transitions of praseodymium(III) complexes in solution media

1992 ◽  
Vol 70 (1) ◽  
pp. 46-54 ◽  
Author(s):  
Sudhindra N. Misra ◽  
Shaun O. Sommerer
Keyword(s):  
Amino Acids ◽  
Quantitative Analysis ◽  
Present Article ◽  
Oscillator Strength ◽  
Schiff Bases ◽  
High Sensitivity ◽  
Structural Features ◽  
Absorption Spectrophotometry ◽  
Nucleic Bases ◽  
Hypersensitive Transitions

The absorption difference, comparative absorption spectrophotometry, and quantitative analysis of 4f–4f spectra of praseodymium(III) complexes with ligands possessing varying structural features and binding capabilities have clearly indicated that the 3H4 → 3P2, 3H4 → 3P1, 3H4 → 3P0, and 3H4 → 1D2 transitions exhibit substantial intensification as well as a wide variation of oscillator strength. Since these transitions do not follow the selection rules they cannot be considered hypersensitive. Ligands such as β-diketones, Schiff bases, amino acids, diols, nucleic bases, nucleosides, nucleotides, alkoxides, and haloacetates with widely differing binding features in terms of coordinating ability, denticity, and normalized bite appear to induce, to varying degrees, high sensitivity in these non-hypersensitive transitions. The present article reports the high sensitivity of the 3H4 → 3P2, 3P1, 3P0, and 1D2, transitions of Pr(III) by considering the solution spectra of 173 species. This unique sensitivity is termed Ligand Mediated Pseudohypersensitivity. Keywords: praseodymium(III), 4f–4f transitions, hypersensitive transitions.

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