scholarly journals In situ modulation of gold nanorod's surface charge drives the growth of end-to-end assemblies from dimers to large networks that enhance single-molecule fluorescence by 10 000-fold

2020 ◽  
Vol 2 (7) ◽  
pp. 2688-2692
Author(s):  
Ashish Kar ◽  
Varsha Thambi ◽  
Diptiranjan Paital ◽  
Saumyakanti Khatua
Keyword(s):  
Surface Charge ◽  
Single Molecule ◽  
Hot Spots ◽  
Plasmonic Nanostructures ◽  
Single Molecule Fluorescence ◽  
Large Networks ◽  
End To End

End-to-end assemblies of anisotropic plasmonic nanostructures with small nanogaps are of great interest as they create strong hot spots for enhancing weak fluorescence and/or scattering of molecules.

scholarly journals In situ genotyping of a pooled strain library after characterizing complex phenotypes

2017 ◽  
Author(s):  
Michael J. Lawson ◽  
Daniel Camsund ◽  
Jimmy Larsson ◽  
Özden Baltekin ◽  
David Fange ◽  
...  
Keyword(s):  
Fluorescence Imaging ◽  
Single Molecule ◽  
Single Molecule Fluorescence ◽  
Detailed Characterization ◽  
E Coli ◽  
Complex Phenotypes

So far, it has not been possible to perform advanced microscopy on pool generated strain libraries and at the same time know each strain’s genotype. We have overcome this barrier by identifying the genotypes for individual cells in situ after a detailed characterization of the phenotype. The principle is demonstrated by single molecule fluorescence imaging of E. coli strains harboring barcoded plasmids that express a sgRNA which suppress different genes through dCas9.

scholarly journals Prospects for plasmonic hot spots in single molecule SERS towards the chemical imaging of live cells

2015 ◽  
Vol 17 (33) ◽  
pp. 21072-21093 ◽  
Author(s):  
Darya Radziuk ◽  
Helmuth Moehwald
Keyword(s):  
Single Molecule ◽  
Hot Spots ◽  
Chemical Imaging ◽  
Local Effect ◽  
Live Cells ◽  
Plasmonic Nanostructures ◽  
Surface Enhanced Raman ◽  
Sharp Edges ◽  
Enhanced Raman Scattering ◽  
Analyte Detection

Single molecule surface enhanced Raman scattering (SM-SERS) is a highly local effect occurring at sharp edges, interparticle junctions and crevices or other geometries with a sharp nanoroughness of plasmonic nanostructures (“hot spots”) for an analyte detection.

scholarly journals Alpha-satellite RNA transcripts are repressed by centromere–nucleolus associations

eLife ◽  
2020 ◽  
Vol 9 ◽  
Author(s):  
Leah Bury ◽  
Brittania Moodie ◽  
Jimmy Ly ◽  
Liliana S McKay ◽  
Karen HH Miga ◽  
...  
Keyword(s):  
Rna Polymerase Ii ◽  
Cell Lines ◽  
Single Molecule ◽  
Alpha Satellite ◽  
Satellite Rna ◽  
Single Molecule Fluorescence ◽  
Chromatin Dynamics ◽  
Rna Transcripts ◽  
Non Coding Rnas

Although originally thought to be silent chromosomal regions, centromeres are instead actively transcribed. However, the behavior and contributions of centromere-derived RNAs have remained unclear. Here, we used single-molecule fluorescence in-situ hybridization (smFISH) to detect alpha-satellite RNA transcripts in intact human cells. We find that alpha-satellite RNA-smFISH foci levels vary across cell lines and over the cell cycle, but do not remain associated with centromeres, displaying localization consistent with other long non-coding RNAs. Alpha-satellite expression occurs through RNA polymerase II-dependent transcription, but does not require established centromere or cell division components. Instead, our work implicates centromere–nucleolar interactions as repressing alpha-satellite expression. The fraction of nucleolar-localized centromeres inversely correlates with alpha-satellite transcripts levels across cell lines and transcript levels increase substantially when the nucleolus is disrupted. The control of alpha-satellite transcripts by centromere-nucleolar contacts provides a mechanism to modulate centromere transcription and chromatin dynamics across diverse cell states and conditions.

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