scholarly journals The rapid diagnosis and effective inhibition of coronavirus using spike antibody attached gold nanoparticles

2021 ◽  
Author(s):  
Avijit Pramanik ◽  
Ye Gao ◽  
Shamily Patibandla ◽  
Dipanwita Mitra ◽  
Martin G. McCandless ◽  
...  
Keyword(s):  
Gold Nanoparticles ◽  
Severe Acute Respiratory Syndrome ◽  
Rapid Diagnosis ◽  
Effective Inhibition

Severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2), the cause of the coronavirus disease that began in 2019 (COVID-19), has been responsible for 1.4 million deaths worldwide as of 13 November 2020.

scholarly journals Peanut-Shaped Gold Nanoparticles with Shells of Ceragenin CSA-131 Display the Ability to Inhibit Ovarian Cancer Growth In Vitro and in a Tumor Xenograft Model

Cancers ◽  
2021 ◽  
Vol 13 (21) ◽  
pp. 5424
Author(s):  
Ewelina Piktel ◽  
Ilona Oscilowska ◽  
Łukasz Suprewicz ◽  
Joanna Depciuch ◽  
Natalia Marcińczyk ◽  
...  
Keyword(s):  
Ovarian Cancer ◽  
Gold Nanoparticles ◽  
Xenograft Model ◽  
Tumor Xenograft ◽  
Assisted Delivery ◽  
Anti Cancer ◽  
Effective Inhibition ◽  
Cancerous Cells

Gold nanoparticles-assisted delivery of antineoplastics into cancerous cells is presented as an effective approach for overcoming the limitations of systemic chemotherapy. Although ceragenins show great potential as anti-cancer agents, in some tumors, effective inhibition of cancer cells proliferation requires application of ceragenins at doses within their hemolytic range. For the purpose of toxicity/efficiency ratio control, peanut-shaped gold nanoparticles (AuP NPs) were functionalized with a shell of ceragenin CSA-131 and the cytotoxicity of AuP@CSA-131 against ovarian cancer SKOV-3 cells and were then analyzed. In vivo efficiency of intravenously and intratumorally administered CSA-131 and AuP@CSA-131 was examined using a xenograft ovarian cancer model. Serum parameters were estimated using ELISA methods. Comparative analysis revealed that AuP@CSA-131 exerted stronger anti-cancer effects than free ceragenin, which was determined by enhanced ability to induce caspase-dependent apoptosis and autophagy processes via reactive oxygen species (ROS)-mediated pathways. In an animal study, AuP@CSA-131 was characterized by delayed clearance and prolonged blood circulation when compared with free ceragenin, as well as enhanced anti-tumor efficiency, particularly when applied intratumorally. Administration of CSA-131 and AuP@CSA-131 prevented the inflammatory response associated with cancer development. These results present the possibility of employing non-spherical gold nanoparticles as an effective nanoplatform for the delivery of antineoplastics for the treatment of ovarian malignancy.

scholarly journals Comparison of Rapid Antigen Tests for COVID-19

Viruses ◽  
2020 ◽  
Vol 12 (12) ◽  
pp. 1420
Author(s):  
Seiya Yamayoshi ◽  
Yuko Sakai-Tagawa ◽  
Michiko Koga ◽  
Osamu Akasaka ◽  
Ichiro Nakachi ◽  
...  
Keyword(s):  
Severe Acute Respiratory Syndrome ◽  
Quantitative Pcr ◽  
Reverse Transcription ◽  
Infectious Virus ◽  
Virus Isolation ◽  
Rapid Diagnosis ◽  
Lateral Flow ◽  
Viral Antigens ◽  
Reverse Transcription Quantitative Pcr ◽  
Antigen Tests

Reverse transcription-quantitative PCR (RT-qPCR)-based tests are widely used to diagnose coronavirus disease 2019 (COVID-19). As a result that these tests cannot be done in local clinics where RT-qPCR testing capability is lacking, rapid antigen tests (RATs) for COVID-19 based on lateral flow immunoassays are used for rapid diagnosis. However, their sensitivity compared with each other and with RT-qPCR and infectious virus isolation has not been examined. Here, we compared the sensitivity among four RATs by using severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) isolates and several types of COVID-19 patient specimens and compared their sensitivity with that of RT-qPCR and infectious virus isolation. Although the RATs read the samples containing large amounts of virus as positive, even the most sensitive RAT read the samples containing small amounts of virus as negative. Moreover, all RATs tested failed to detect viral antigens in several specimens from which the virus was isolated. The current RATs will likely miss some COVID-19 patients who are shedding infectious SARS-CoV-2.

A Study on Understanding Potential Gold and Silver Nanoparticle : An Overview

2020 ◽  
pp. 2150009
Author(s):  
Shadeeb Hossain
Keyword(s):  
Gold Nanoparticles ◽  
Silver Nanoparticles ◽  
Optical Property ◽  
Severe Acute Respiratory Syndrome ◽  
Silver Nanoparticle ◽  
Diagnostic Tool ◽  
Transmission Rate ◽  
Electrochemical Techniques ◽  
Gold And Silver Nanoparticles ◽  
Rapid Transmission

This paper highlights on the coronavirus outbreak caused by severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2). At the time of writing this paper, there has been over 6 million confirmed cases worldwide. It is a person–person transmittable infection but there have been cases of asymptomatic carriers. Hence, development of an effective biosensing diagnostic tool can curb its rapid transmission rate. The first part of the paper highlights on the SARS-CoV-2 structure and its resemblance to SARS-CoV. The second part of the paper analyzes on the potential application of gold and silver nanoparticles to generate a red shift that had enhanced the calorimetric property of the MERS-CoV analysis due to transition in its optical property. Other electrochemical techniques that utilized the application of gold nanoparticles are also reviewed. Gold and silver nanoparticles (AuNP and Ag NP) can accelerate the sensitivity upon electrodeposition on the diagnostic tool.

scholarly journals Rapid Diagnosis of a Coronavirus Associated with Severe Acute Respiratory Syndrome (SARS)

2003 ◽  
Vol 49 (6) ◽  
pp. 953-955 ◽  
Author(s):  
Leo L M Poon ◽  
On Kei Wong ◽  
Winsie Luk ◽  
Kwok Yung Yuen ◽  
Joseph S M Peiris ◽  
...  
Keyword(s):  
Severe Acute Respiratory Syndrome ◽  
Rapid Diagnosis

The Antibacterial Effect of Bacteriophage-Like Gold Nanoparticles

NANO ◽  
2021 ◽  
pp. 2150075
Author(s):  
Xiuzhen Huang ◽  
Benben Lu ◽  
Yingxian Zhao ◽  
Zhiqiang Wang ◽  
Hongwei Wang ◽  
...  
Keyword(s):  
Gold Nanoparticles ◽  
Pathogenic Bacteria ◽  
Near Infrared ◽  
Antibacterial Effect ◽  
Environmental Engineering ◽  
Oxygen Species ◽  
Surrounding Environment ◽  
Low Concentrations ◽  
Effective Inhibition

The growth of pathogenic bacteria seriously threatens human health. Effective inhibition of pathogenic bacteria in the human body and the surrounding environment is of great urgency. In this work, we propose a strategy in preparing bacteriophage-like gold nanoparticles (B-AuNPs) for antibacterial applications. B-AuNPs were prepared by multiple in-situ reducing reactions on the surface of AuNPs. It was shown that the secondary-reduced AuNPs (B1-AuNPs) have a bacteriophage shaped morphology. Under near infrared laser, B1-AuNPs could almost completely inhibit bacterial growth at low concentrations within 5 min, due to the combined effects of increased temperature and reactive oxygen species (ROS). Moreover, B-AuNPs can be easily separated from the dead bacteria and the recycled B-AuNPs remain the high antibacterial efficiency. It is concluded that B-AuNPs have great potentials in the antibacterial researches for biocontrol, biomedicine and environmental engineering.

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