scholarly journals Rapid Diagnosis of a Coronavirus Associated with Severe Acute Respiratory Syndrome (SARS)

2003 ◽  
Vol 49 (6) ◽  
pp. 953-955 ◽  
Author(s):  
Leo L M Poon ◽  
On Kei Wong ◽  
Winsie Luk ◽  
Kwok Yung Yuen ◽  
Joseph S M Peiris ◽  
...  
Keyword(s):  
Severe Acute Respiratory Syndrome ◽  
Rapid Diagnosis

scholarly journals The rapid diagnosis and effective inhibition of coronavirus using spike antibody attached gold nanoparticles

2021 ◽  
Author(s):  
Avijit Pramanik ◽  
Ye Gao ◽  
Shamily Patibandla ◽  
Dipanwita Mitra ◽  
Martin G. McCandless ◽  
...  
Keyword(s):  
Gold Nanoparticles ◽  
Severe Acute Respiratory Syndrome ◽  
Rapid Diagnosis ◽  
Effective Inhibition

Severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2), the cause of the coronavirus disease that began in 2019 (COVID-19), has been responsible for 1.4 million deaths worldwide as of 13 November 2020.

scholarly journals Comparison of Rapid Antigen Tests for COVID-19

Viruses ◽  
2020 ◽  
Vol 12 (12) ◽  
pp. 1420
Author(s):  
Seiya Yamayoshi ◽  
Yuko Sakai-Tagawa ◽  
Michiko Koga ◽  
Osamu Akasaka ◽  
Ichiro Nakachi ◽  
...  
Keyword(s):  
Severe Acute Respiratory Syndrome ◽  
Quantitative Pcr ◽  
Reverse Transcription ◽  
Infectious Virus ◽  
Virus Isolation ◽  
Rapid Diagnosis ◽  
Lateral Flow ◽  
Viral Antigens ◽  
Reverse Transcription Quantitative Pcr ◽  
Antigen Tests

Reverse transcription-quantitative PCR (RT-qPCR)-based tests are widely used to diagnose coronavirus disease 2019 (COVID-19). As a result that these tests cannot be done in local clinics where RT-qPCR testing capability is lacking, rapid antigen tests (RATs) for COVID-19 based on lateral flow immunoassays are used for rapid diagnosis. However, their sensitivity compared with each other and with RT-qPCR and infectious virus isolation has not been examined. Here, we compared the sensitivity among four RATs by using severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) isolates and several types of COVID-19 patient specimens and compared their sensitivity with that of RT-qPCR and infectious virus isolation. Although the RATs read the samples containing large amounts of virus as positive, even the most sensitive RAT read the samples containing small amounts of virus as negative. Moreover, all RATs tested failed to detect viral antigens in several specimens from which the virus was isolated. The current RATs will likely miss some COVID-19 patients who are shedding infectious SARS-CoV-2.

scholarly journals Nucleic Acid-Based Diagnostic Tests for the Detection SARS-CoV-2: An Update

Diagnostics ◽  
2021 ◽  
Vol 11 (1) ◽  
pp. 53
Author(s):  
Choo Yee Yu ◽  
Kok Gan Chan ◽  
Chan Yean Yean ◽  
Geik Yong Ang
Keyword(s):  
Nucleic Acid ◽  
Severe Acute Respiratory Syndrome ◽  
Healthcare System ◽  
Diagnostic Tests ◽  
Rapid Diagnosis ◽  
Future Perspectives ◽  
Rt Pcr ◽  
Advantages And Disadvantages ◽  
Diagnostic Panel ◽  
And Performance

The coronavirus disease 2019 (COVID-19) caused by severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) began as a cluster of pneumonia cases in Wuhan, China before spreading to over 200 countries and territories on six continents in less than six months. Despite rigorous global containment and quarantine efforts to limit the transmission of the virus, COVID-19 cases and deaths have continued to increase, leaving devastating impacts on the lives of many with far-reaching effects on the global society, economy and healthcare system. With over 43 million cases and 1.1 million deaths recorded worldwide, accurate and rapid diagnosis continues to be a cornerstone of pandemic control. In this review, we aim to present an objective overview of the latest nucleic acid-based diagnostic tests for the detection of SARS-CoV-2 that have been authorized by the Food and Drug Administration (FDA) under emergency use authorization (EUA) as of 31 October 2020. We systematically summarize and compare the principles, technologies, protocols and performance characteristics of amplification- and sequencing-based tests that have become alternatives to the CDC 2019-nCoV Real-Time RT-PCR Diagnostic Panel. We highlight the notable features of the tests including authorized settings, along with the advantages and disadvantages of the tests. We conclude with a brief discussion on the current challenges and future perspectives of COVID-19 diagnostics.

Solid-phase immunoelectron microscopy for rapid diagnosis of enteroviruses

1984 ◽  
Vol 42 ◽  
pp. 226-227 ◽  
Author(s):  
K. Pegg-Feige ◽  
F. W. Doane
Keyword(s):  
Electron Microscopy ◽  
Cell Culture ◽  
Immunoelectron Microscopy ◽  
Detection Method ◽  
Solid Phase ◽  
Protein A ◽  
Plant Viruses ◽  
Rapid Diagnosis ◽  
Virus Diagnosis ◽  
Antiviral Antibody

Immunoelectron microscopy (IEM) applied to rapid virus diagnosis offers a more sensitive detection method than direct electron microscopy (DEM), and can also be used to serotype viruses. One of several IEM techniques is that introduced by Derrick in 1972, in which antiviral antibody is attached to the support film of an EM specimen grid. Originally developed for plant viruses, it has recently been applied to several animal viruses, especially rotaviruses. We have investigated the use of this solid phase IEM technique (SPIEM) in detecting and identifying enteroviruses (in the form of crude cell culture isolates), and have compared it with a modified “SPIEM-SPA” method in which grids are coated with protein A from Staphylococcus aureus prior to exposure to antiserum.

Perceptions about Severe Acute Respiratory Syndrome Experience Scales

2006 ◽  
Author(s):  
Robert G. Maunder ◽  
William J. Lancee ◽  
Kenneth E. Balderson ◽  
Jocelyn P. Bennett ◽  
Bjug Borgundvaag ◽  
...  
Keyword(s):  
Severe Acute Respiratory Syndrome

99mTc Technetium Sulfide and the Scintillation Camera in the Rapid Diagnosis of Pericardial Effusion

1971 ◽  
Vol 10 (02) ◽  
pp. 122-128
Author(s):  
W. H. Blahd ◽  
M. A. Winston ◽  
G. T. Krishnamurthy ◽  
P. B. Thomas ◽  
E. Weiss
Keyword(s):  
Pericardial Effusion ◽  
Patient Positioning ◽  
Rapid Diagnosis ◽  
Scintillation Camera ◽  
Technical Error ◽  
Technetium 99M ◽  
Sodium Pertechnetate ◽  
Specific Uptake ◽  
Speed Accuracy

SummaryBecause of its speed, accuracy, and reproducibility, radioisotopic angiocardiography appears to be the method of choice for the diagnosis of pericardial effusion. Technetium-99m sulfide is preferable to 99mTc sodium pertechnetate in these studies for the following reasons: (1) its specific uptake by the liver aids both in patient positioning and in the accuracy of diagnosis; and (2), studies can be repeated within 15—20 minutes should there be equiment or technical error.

Rapid detection of Rotavirus antigen in stool sample of acute diarrheic children

2012 ◽  
Vol 6 (1) ◽  
pp. 11-13
Author(s):  
Sushmita Roy ◽  
S.M. Shamsuzzaman ◽  
K.Z. Mamun
Keyword(s):  
Stool Sample ◽  
Rapid Detection ◽  
Cross Sectional Study ◽  
Rapid Diagnosis ◽  
Age Group ◽  
College Hospital ◽  
Cross Sectional ◽  
Rotavirus Infection ◽  
Medical College ◽  
Stool Samples

Rotavirus is one of the leading causes of pediatric diarrhea globally. Accurate and rapid diagnosis of Rotavirus diarrhea should reduce unnecessary use of antibiotics and ultimately reduce drug resistance. Study was designed for rapid diagnosis of Rotavirus antigen in stool sample by ICT (Immunochromatographic test) as well as to observe the seasonal variation of rotavirus infection. This cross sectional study was carried out in the department of Microbiology, Dhaka Medical College from January 2011 to December 2011. Eighty stool samples were collected from Dhaka Shishu Hospital and Dhaka Medical College Hospital. All samples were tested for rotavirus antigen by ICT. Among 80 patients, 42 (52.5%) samples were positive for rotavirus antigen. Among these 42 positive samples, 30 (71.43%) were from 0-12 months of age group, 10 (23.81%) from 13 to 24 months of age group and rest 2 (4.76%) from 25 to 36 months of age group. Rotavirus Ag was detected in stool samples from January to April and another peak episode from October to December. Considering the importance of Rotavirus associated diarrhea, rapid detection of Rotavirus infection in human is substantially needed and should be routinely practiced.DOI: http://dx.doi.org/10.3329/bjmm.v6i1.19354 Bangladesh J Med Microbiol 2012; 06(01): 11-13

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