scholarly journals Evaluation of a Newly Designed Immunochromatographic Test using Gold Nanoparticles and Recombinant Antigen gra7 for Rapid Diagnosis of Human Toxoplasmosis

2020 ◽  
Vol 14 (1) ◽  
pp. 101-115
Author(s):  
Hassan Morovati ◽  
Seyyed javad Seyyedtabaei ◽  
Mehrdad Gholamzad ◽  
◽  
◽  
...  
Keyword(s):  
Gold Nanoparticles ◽  
Recombinant Antigen ◽  
Rapid Diagnosis ◽  
Immunochromatographic Test

scholarly journals The rapid diagnosis and effective inhibition of coronavirus using spike antibody attached gold nanoparticles

2021 ◽  
Author(s):  
Avijit Pramanik ◽  
Ye Gao ◽  
Shamily Patibandla ◽  
Dipanwita Mitra ◽  
Martin G. McCandless ◽  
...  
Keyword(s):  
Gold Nanoparticles ◽  
Severe Acute Respiratory Syndrome ◽  
Rapid Diagnosis ◽  
Effective Inhibition

Severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2), the cause of the coronavirus disease that began in 2019 (COVID-19), has been responsible for 1.4 million deaths worldwide as of 13 November 2020.

scholarly journals Rapid Diagnosis of Malaria by Antigen Detection

2009 ◽  
Vol 3 (1) ◽  
pp. 14-16
Author(s):  
Mesbah Uddin Ahmed ◽  
Md Akram Hossain ◽  
AKM Shamsuzzaman ◽  
Md Murshed Alam ◽  
Abdul Hossain Khan ◽  
...  
Keyword(s):  
Thin Films ◽  
Sensitivity And Specificity ◽  
Standard Method ◽  
Gold Standard ◽  
Rapid Diagnosis ◽  
Healthy Controls ◽  
Immunochromatographic Test ◽  
Gold Standard Method ◽  
Malaria Antigen ◽  
Age And Sex

The study was conducted to evaluate the sensitivity and specificity of Immunochromatographic test (ICT) for antigen, using microscopy as the "gold standard" method for diagnosis of malaria. A total of 98 clinically suspected malaria patients and another 30 age and sex-matched healthy controls were included in this study. Thick and thin films were also prepared and examined under microscope as well as Immunochromatographic test (ICT) was performed for malaria antigen. Sensitivity and specificity of ICT for antigen were 93.22% and 94.87% respectively. Keywords: Detection of malaria antigen, Immunochromatographic test   doi: 10.3329/bjmm.v3i1.2965 Bangladesh J Med Microbiol 2009; 03 (01): 14-16

ANTIBODY CONJUGATES WITH GOLD NANOPARTICLES AS A COMPONENT OF IMMUNOCHROMATOGRAPHIC DIAGNOSTICUMS: THE INFLUENCE OF SYNTHESIS CONDITIONS ON THE COMPOSITION AND REACTIVITY

2020 ◽  
pp. 180-181
Author(s):  
N. Byzova ◽  
A. Zherdev ◽  
B. Dzantiev
Keyword(s):  
Gold Nanoparticles ◽  
Immunochromatographic Test ◽  
C Reactive Protein ◽  
Synthesis Conditions ◽  
Reactive Protein ◽  
Test Systems ◽  
Antibody Conjugates ◽  
Analytical Reagents ◽  
D Dimer

A series of preparations of gold nanoparticles with diameters from 13 to 60 nm and their conjugates with antibodies (murine immunoglobulins of class G) of different composition were obtained. The composition of the conjugates and the amount of antibodies that retain their reactivity in an immobilized form are characterized. Using the example of immunochromatographic test systems for the detection of D-dimer and C-reactive protein, the effectiveness of conjugates as analytical reagents is compared.

Rapid detection of Shiga toxin type II using lateral flow immunochromatography test strips of colorimetry and fluorimetry

The Analyst ◽  
2020 ◽  
Vol 145 (1) ◽  
pp. 76-82 ◽  
Author(s):  
Tian Lu ◽  
Kai-Di Zhu ◽  
Chao Huang ◽  
Tian Wen ◽  
Yong-Jun Jiao ◽  
...  
Keyword(s):  
Quantum Dots ◽  
Gold Nanoparticles ◽  
Shiga Toxin ◽  
Rapid Detection ◽  
Lateral Flow ◽  
Cdte Quantum Dots ◽  
Type Ii ◽  
Immunochromatographic Test ◽  
Test Strips

Two types of lateral flow immunochromatographic test strips (LFITS) using gold nanoparticles and fluorescent CdTe quantum dots (QDs) as signal labels, respectively, were developed for Shiga toxin type II (STX2) assays.

A novel enhancement assay for immunochromatographic test strips using gold nanoparticles

2006 ◽  
Vol 385 (8) ◽  
pp. 1414-1420 ◽  
Author(s):  
Ryo Tanaka ◽  
Teruko Yuhi ◽  
Naoki Nagatani ◽  
Tatsuro Endo ◽  
Kagan Kerman ◽  
...  
Keyword(s):  
Gold Nanoparticles ◽  
Immunochromatographic Test ◽  
Test Strips

scholarly journals Evaluation of a New Immunochromatographic Test Using Recombinant Antigen B8/1 for Diagnosis of Cystic Echinococcosis

2015 ◽  
Vol 53 (12) ◽  
pp. 3859-3863 ◽  
Author(s):  
Saul J. Santivañez ◽  
Mary L. Rodriguez ◽  
Silvia Rodriguez ◽  
Yashuito Sako ◽  
Agathe Nkouawa ◽  
...  
Keyword(s):  
Cystic Echinococcosis ◽  
High Specificity ◽  
Recombinant Antigen ◽  
Enzyme Linked Immunosorbent Assay ◽  
Parasitic Infections ◽  
Clinical Settings ◽  
Immunochromatographic Test ◽  
Serum Samples ◽  
Content Type ◽  
Elisa Format

Diagnosis of cystic echinococcosis (CE) is based on the identification of the cyst(s) by imaging, using immunodiagnostic tests mainly as complementary tools in clinical settings. Among the antigens used for immunodiagnosis, previous studies described a good performance of the recombinant antigen B8/1 (rAgB) in an enzyme-linked immunosorbent assay (ELISA) format; however, in remote parts of areas where the disease is endemic, the implementation of an ELISA is difficult, so a more simple, rapid, and reliable method such as the immunochromatographic test (ICT) is required. In this study, using a set of 50 serum samples from patients with surgically confirmed CE, we compared the performance of an ICT and that of an ELISA using the rAgB. The overall sensitivities of ICT and ELISA were not statistically different (78% versus 72%;P= 0.36). The overall agreement between both tests was moderate (κ = 0.41;P< 0.01). Concordance between ICT and ELISA was substantial or almost perfect for patients with liver involvement (κ = 0.65;P< 0.001) and patients with more than one hydatid cyst (κ = 0.82;P< 0.001), respectively. Moreover, specificity analysis using a total of 88 serum samples from healthy individuals (n= 20) and patients (n= 68) with other parasitic infections revealed that ICT had a specificity of 89.8%. ICT and ELISA had similar performance for the detection of specific antibodies toE. granulosus, and ICT had a high specificity, opening the possibility of using ICT as a screening tool in rural settings.

scholarly journals Antigen-capture ELISA and immunochromatographic test strip to detect the H9N2 subtype avian influenza virus rapidly based on monoclonal antibodies

2021 ◽  
Vol 18 (1) ◽  
Author(s):  
Yixin Xiao ◽  
Fan Yang ◽  
Fumin Liu ◽  
Hangping Yao ◽  
Nanping Wu ◽  
...  
Keyword(s):  
Influenza Virus ◽  
Monoclonal Antibodies ◽  
Detection Limit ◽  
Avian Influenza ◽  
Avian Influenza Virus ◽  
Rapid Diagnosis ◽  
Enzyme Linked Immunosorbent Assay ◽  
Immunochromatographic Test ◽  
H9n2 Subtype ◽  
Antigen Capture

Abstract Background The H9N2 subtype of avian influenza virus (AIV) has become the most widespread subtype of AIV among birds in Asia, which threatens the poultry industry and human health. Therefore, it is important to establish methods for the rapid diagnosis and continuous surveillance of H9N2 subtype AIV. Methods In this study, an antigen-capture enzyme-linked immunosorbent assay (AC-ELISA) and a colloidal gold immunochromatographic test (ICT) strip using monoclonal antibodies (MAbs) 3G4 and 2G7 were established to detect H9N2 subtype AIV. Results The AC-ELISA method and ICT strip can detect H9N2 subtype AIV quickly, and do not cross-react with other subtype AIVs or other viruses. The detection limit of AC-ELISA was a hemagglutinin (HA) titer of 4 for H9N2 subtype AIV per 100 μl sample, and the limit of detection of the HA protein of AIV H9N2 was 31.5 ng/ml. The ICT strip detection limit was an HA titer of 4 for H9N2 subtype AIV per 100 μl sample. Moreover, both detection methods exhibited good reproducibility and repeatability, with coefficients of variation < 5%. For detection in 200 actual poultry samples, the sensitivities and specificities of AC-ELISA were determined as 93.2% and 98.1%, respectively. The sensitivities and specificities of the ICT strips were determined as 90.9% and 97.4%, respectively. Conclusions The developed AC-ELISA and ICT strips displayed high specificity, sensitivity, and stability, making them suitable for rapid diagnosis and field investigation of H9N2 subtype AIV.

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