The Antibacterial Effect of Bacteriophage-Like Gold Nanoparticles

NANO ◽  
2021 ◽  
pp. 2150075
Author(s):  
Xiuzhen Huang ◽  
Benben Lu ◽  
Yingxian Zhao ◽  
Zhiqiang Wang ◽  
Hongwei Wang ◽  
...  
Keyword(s):  
Gold Nanoparticles ◽  
Pathogenic Bacteria ◽  
Near Infrared ◽  
Antibacterial Effect ◽  
Environmental Engineering ◽  
Oxygen Species ◽  
Surrounding Environment ◽  
Low Concentrations ◽  
Effective Inhibition

The growth of pathogenic bacteria seriously threatens human health. Effective inhibition of pathogenic bacteria in the human body and the surrounding environment is of great urgency. In this work, we propose a strategy in preparing bacteriophage-like gold nanoparticles (B-AuNPs) for antibacterial applications. B-AuNPs were prepared by multiple in-situ reducing reactions on the surface of AuNPs. It was shown that the secondary-reduced AuNPs (B1-AuNPs) have a bacteriophage shaped morphology. Under near infrared laser, B1-AuNPs could almost completely inhibit bacterial growth at low concentrations within 5 min, due to the combined effects of increased temperature and reactive oxygen species (ROS). Moreover, B-AuNPs can be easily separated from the dead bacteria and the recycled B-AuNPs remain the high antibacterial efficiency. It is concluded that B-AuNPs have great potentials in the antibacterial researches for biocontrol, biomedicine and environmental engineering.

scholarly journals Antibacterial Effect of Chitosan–Gold Nanoparticles and Computational Modeling of the Interaction between Chitosan and a Lipid Bilayer Model

Nanomaterials ◽  
2020 ◽  
Vol 10 (12) ◽  
pp. 2340
Author(s):  
M. G. Fuster ◽  
M. G. Montalbán ◽  
G. Carissimi ◽  
B. Lima ◽  
G. E. Feresin ◽  
...  
Keyword(s):  
Gold Nanoparticles ◽  
Simulation Model ◽  
Pathogenic Bacteria ◽  
Antibacterial Effect ◽  
Resistance Mechanisms ◽  
Target Point ◽  
Systematic Analysis ◽  
Bacterial Membranes ◽  
Synthesis Parameters ◽  
Cell Wall Disruption

Pathogenic bacteria have the ability to develop antibiotic resistance mechanisms. Their action consists mainly in the production of bacterial enzymes that inactivate antibiotics or the appearance of modifications that prevent the arrival of the drug at the target point or the alteration of the target point itself, becoming a growing problem for health systems. Chitosan–gold nanoparticles (Cs-AuNPs) have been shown as effective bactericidal materials avoiding damage to human cells. In this work, Cs-AuNPs were synthesized using chitosan as the reducing agent, and a systematic analysis of the influence of the synthesis parameters on the size and zeta potential of the Cs-AuNPs and their UV-vis spectra was carried out. We used a simulation model to characterize the interaction of chitosan with bacterial membranes, using a symmetric charged bilayer and two different chitosan models with different degrees of the chitosan amine protonation as a function of pH, with the aim to elucidate the antibacterial mechanism involving the cell wall disruption. The Cs-AuNP antibacterial activity was evaluated to check the simulation model.

Gold nanoparticles for the in situ polymerization of near-infrared responsive hydrogels based on fibrin

2019 ◽  
Vol 100 ◽  
pp. 306-315 ◽  
Author(s):  
Clara Escudero-Duch ◽  
Francisco Martin-Saavedra ◽  
Martin Prieto ◽  
Silvia Sanchez-Casanova ◽  
Daniel Lopez ◽  
...  
Keyword(s):  
Gold Nanoparticles ◽  
Near Infrared ◽  
In Situ Polymerization ◽  
Responsive Hydrogels

scholarly journals A Sample Preparation Technique Using Biocompatible Composites for Biomedical Applications

Molecules ◽  
2019 ◽  
Vol 24 (7) ◽  
pp. 1321 ◽  
Author(s):  
Huifang Liu ◽  
Geun Su Noh ◽  
Yange Luan ◽  
Zhen Qiao ◽  
Bonhan Koo ◽  
...  
Keyword(s):  
Nucleic Acid ◽  
Biomedical Applications ◽  
Pathogenic Bacteria ◽  
Fold Increase ◽  
Preparation Technique ◽  
Diagnostic Systems ◽  
Low Concentrations ◽  
Sample Enrichment ◽  
Nucleic Acid Preparation

Infectious diseases, especially pathogenic infections, are a growing threat to public health worldwide. Since pathogenic bacteria usually exist in complex matrices at very low concentrations, the development of technology for rapid, convenient, and biocompatible sample enrichment is essential for sensitive diagnostics. In this study, a cucurbit[6]uril (CB) supermolecular decorated amine-functionalized diatom (DA) composite was fabricated to support efficient sample enrichment and in situ nucleic acid preparation from enriched pathogens and cells. CB was introduced to enhance the rate and effectiveness of pathogen absorption using the CB–DA composite. This novel CB–DA composite achieved a capture efficiency of approximately 90% at an Escherichia coli concentration of 106 CFU/mL within 3 min. Real-time PCR analyses of DNA samples recovered using the CB–DA enrichment system showed a four-fold increase in the early amplification signal strength, and this effective method for capturing nucleic acid might be useful for preparing samples for diagnostic systems.

Silicon nanowires decorated with gold nanoparticles via in situ reduction for photoacoustic imaging-guided photothermal cancer therapy

2019 ◽  
Vol 7 (28) ◽  
pp. 4393-4401 ◽  
Author(s):  
Lina Sun ◽  
Ying Chen ◽  
Fei Gong ◽  
Qian Dang ◽  
Guangzhen Xiang ◽  
...  
Keyword(s):  
Gold Nanoparticles ◽  
Cancer Therapy ◽  
Solid Tumors ◽  
Silicon Nanowires ◽  
Photothermal Therapy ◽  
Near Infrared ◽  
Photoacoustic Imaging ◽  
In Situ Reduction ◽  
Great Progress

Photothermal therapy (PTT) in the first near-infrared (NIR-I) window has made great progress in the treatment of solid tumors, while only a few PTT agents in the second NIR (NIR-II) region have been studied.

scholarly journals Gold Nanoparticles and Graphene Oxide Flakes Synergistic Partaking in Cytosolic Bactericidal Augmentation: Role of ROS and NOX2 Activity

2021 ◽  
Vol 9 (1) ◽  
pp. 101
Author(s):  
Osamah Al Rugaie ◽  
Majid Jabir ◽  
Rua Kadhim ◽  
Esraa Karsh ◽  
Ghassan M. Sulaiman ◽  
...  
Keyword(s):  
Gold Nanoparticles ◽  
Graphene Oxide ◽  
Pathogenic Bacteria ◽  
Feedback Mechanism ◽  
Synergistic Action ◽  
Oxygen Species ◽  
Phagosome Maturation ◽  
Bacterial Strains ◽  
Experimental Conditions

Gold nanoparticles (GNPs) and graphene oxide flakes (GOFs) exerted significantly (p < 0.0001) supportive roles on the phagocytosis bioactivity of the immune cells of phagocytic nature against the Gram-positive and Gram-negative human pathogenic bacteria Staphylococcus aureus and Escherichia coli. Under experimental conditions, upon bacterial exposure, the combined GNPs and GOFs induced significant clearance of bacteria through phagosome maturation (p < 0.0001) from time-points of 6 to 30 min and production of reactive oxygen species (ROS, p < 0.0001) through the NADPH oxidase 2 (NOX2, p < 0.0001)-based feedback mechanism. The effects of the combined presence of GNPs and GOFs on phagocytosis (p < 0.0001) suggested a synergistic action underway, also achieved through elevated signal transduction activity in the bone-marrow-derived macrophages (BMDM, p < 0.0001). The current study demonstrated that GNPs’ and GOFs’ bactericidal assisting potentials could be considered an effective and alternative strategy for treating infections from both positive and negative bacterial strains.

scholarly journals Easy and fast in-situ functionalization of exfoliated 2D black phosphorus with gold nanoparticles

2021 ◽  
Author(s):  
Salvatore Moschetto ◽  
Andrea Ienco ◽  
Gabriele Manca ◽  
Manuel Serrano-Ruiz ◽  
Maurizio Peruzzini ◽  
...  
Keyword(s):  
Gold Nanoparticles ◽  
Black Phosphorus ◽  
Au Nps ◽  
In Situ Functionalization

Heterostructures of single- and few-layer black phosphorus (2D bP) functionalized with gold nanoparticles (Au NPs) have been recently reported in the literature, exploiting their intriguing properties and biocompatibility for catalytic,...

scholarly journals A residue-free approach to water disinfection using catalytic in situ generation of reactive oxygen species

2021 ◽  
Author(s):  
Thomas Richards ◽  
Jonathan H. Harrhy ◽  
Richard J. Lewis ◽  
Alexander G. R. Howe ◽  
Grzegorz M. Suldecki ◽  
...  
Keyword(s):  
Reactive Oxygen Species ◽  
Reactive Oxygen ◽  
Water Disinfection ◽  
Oxygen Species ◽  
In Situ Generation
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