scholarly journals Evaluating the effect of synthesis, isolation, and characterisation variables on reported particle size and dispersity of drug loaded PLGA nanoparticles

2021 ◽  
Author(s):  
Bruna C. Garms ◽  
Hamish Poli ◽  
Darcy Baggley ◽  
Felicity Y. Han ◽  
Andrew K. Whittaker ◽  
...  
Keyword(s):  
Particle Size ◽  
Plga Nanoparticles ◽  
Drug Encapsulation ◽  
Nanoparticle Fabrication

This work provides advice for PLGA-based nanoparticle fabrication and drug encapsulation quantification as well as the minimum required information to be reported allowing reproducibility.

scholarly journals Effect of Chitosan Coating on PLGA Nanoparticles for Oral Delivery of Thymoquinone: In Vitro, Ex Vivo, and Cancer Cell Line Assessments

Coatings ◽  
2020 ◽  
Vol 11 (1) ◽  
pp. 6
Author(s):  
Sultan Alshehri ◽  
Syed Sarim Imam ◽  
Md Rizwanullah ◽  
Khalid Umar Fakhri ◽  
Mohd Moshahid Alam Rizvi ◽  
...  
Keyword(s):  
Breast Cancer ◽  
Particle Size ◽  
Oral Delivery ◽  
Ex Vivo ◽  
Cancer Cell Line ◽  
Entrapment Efficiency ◽  
Plga Nanoparticles ◽  
Cancer Management ◽  
Mcf 7

In the present study, thymoquinone (TQ)-encapsulated chitosan- (CS)-coated poly(d,l-lactide-co-glycolide) (PLGA) nanoparticles (NPs) were formulated using the emulsion evaporation method. NPs were optimized by using 33-QbD approach for improved efficacy against breast cancer. The optimized thymoquinone loaded chitosan coated Poly (d,l-lactide-co-glycolide) nanoparticles (TQ-CS-PLGA-NPs) were successfully characterized by different in vitro and ex vivo experiments as well as evaluated for cytotoxicity in MDA-MB-231 and MCF-7 cell lines. The surface coating of PLGA-NPs was completed by CS coating and there were no significant changes in particle size and entrapment efficiency (EE) observed. The developed TQ-CS-PLGA-NPs showed particle size, polydispersibility index (PDI), and %EE in the range between 126.03–196.71 nm, 0.118–0.205, and 62.75%–92.17%. The high and prolonged TQ release rate was achieved from TQ-PLGA-NPs and TQ-CS-PLGA-NPs. The optimized TQ-CS-PLGA-NPs showed significantly higher mucoadhesion and intestinal permeation compared to uncoated TQ-PLGA-NPs and TQ suspension. Furthermore, TQ-CS-PLGA-NPs showed statistically enhanced antioxidant potential and cytotoxicity against MDA-MB-231 and MCF-7 cells compared to uncoated TQ-PLGA-NPs and pure TQ. On the basis of the above findings, it may be stated that chitosan-coated TQ-PLGA-NPs represent a great potential for breast cancer management.

Remote-Controllable Bone-Targeted Delivery of Estradiol For the Treatment of Ovariectomy-Induced Osteoporosis in Rats

2021 ◽  
Author(s):  
Dehao Fu ◽  
Yuanyuan Guo ◽  
Yongwei Liu ◽  
Chen Shi ◽  
Tingting Wu ◽  
...  
Keyword(s):  
Particle Size ◽  
Bone Tissue ◽  
Targeted Delivery ◽  
Hormone Replacement ◽  
Plga Nanoparticles ◽  
Raw 264.7 Cells ◽  
Diffusion Method ◽  
Tissue Destruction ◽  
Trap 5B ◽  
Post Menopausal

Abstract BackgroundOsteoporosis (OP) is a systemic skeletal disease marked by bone mass reduction and bone tissue destruction. Hormone replacement therapy is an effective treatment for post-menopausal OP, but estrogen has poor tissue selectivity and severe side effects.ResultsIn this study, we constructed a poly(lactic-co-glycolic acid) (PLGA) nanoparticles (NPs)-based drug delivery system to co-load 17β estradiol (E2) and iron oxide (Fe3O4) together, modified with alendronate (AL) to achieve bone targeting and realize a magnetically remote-controllable drug release. The NPs were fabricated through the emulsion solvent diffusion method. The particle size was approximately 200 nm while the encapsulation efficiency of E2 was 58.34 ± 9.21%. The NPs were found to be spherical with a homogenous distribution of particle size. The NPs showed good stability, good biocompatibility, high encapsulation ability of E2 and excellent magnetic properties. The NPs could be effectively taken up by Raw 264.7 cells and were effective in enriching drugs in bone tissue. The co-loaded NPs exposed to an external magnetic field ameliorated OVX-induced bone loss through increased BV/TV, decreased Tb.N and Tb.Sp, improved bone strength, increased PINP and OC, and downregulated CTX and TRAP-5b. The haematological index and histopathological analyses displayed the NPs had less side effects on non-skeletal tissues.ConclusionsThis study presented a remote-controlled release system based on bone-targeted multifunctional NPs and a new potential approach to bone-targeted therapy of OP.

Preparation of All-Trans-Retinoic Acid-Loaded mPEG-PLGA Nanoparticles Using Microfluidic Flow-Focusing Device for Controlled Drug Delivery

NANO ◽  
2020 ◽  
Vol 15 (08) ◽  
pp. 2050101
Author(s):  
Mojdeh Safari ◽  
Amir Amani ◽  
Tajudeen Adebileje ◽  
Jafar Ai ◽  
Seyed Mahdi Rezayat ◽  
...  
Keyword(s):  
Particle Size ◽  
Retinoic Acid ◽  
Plga Nanoparticles ◽  
Process Conditions ◽  
Flow Focusing ◽  
Flow Rates ◽  
Trans Retinoic Acid ◽  
All Trans Retinoic Acid ◽  
Microfluidic Flow ◽  
Minimum Particle Size

In recent years, microfluidic devices present unique advantages for the development of a new generation of nanoparticle synthesis method compared to bulk methods. In this study, we report a microfluidic flow-focusing method for the production of all trans retinoic acid (ATRA)-loaded methoxy poly(ethylene glycol)-poly(lactide-coglycolide) (mPEG-PLGA) nanoparticles (NPs). Box–Behnken experimental design (BBD) was applied to optimize of formulation ingredients and process conditions with minimum particle size, maximum drug loading% (DL%) and encapsulation efficiency% (EE%). Polymer concentration, drug concentration and flow rates of solvent (S) and antisolvent (AS) were selected as independent variables. Based on optimization strategy, minimum particle size achieved shows average (SD) particle size of [Formula: see text][Formula: see text]nm with DL of [Formula: see text][Formula: see text]wt.% and EE of [Formula: see text][Formula: see text]wt.%, respectively. While maximum DL has been reported to be [Formula: see text][Formula: see text]wt.% with particle size of [Formula: see text][Formula: see text]nm and EE of [Formula: see text][Formula: see text]wt.%, respectively. Moreover, the results have shown that the AS/S ratio represents the most significant effect on particle size. Indeed, increasing the AS flow rate directly results in generating smaller particles. The AS/S ratio represents the least significant effect on DL%, such that, at fixed flow rates, higher DL was observed at high concentration of drug and lower concentration of polymer. In conclusion, optimization of the ATRA-loaded mPEG-PLGA NPs by BBD yielded in a favorable drug carrier for ATRA that could provide a new treatment modality for different malignancies.

scholarly journals Ocular pharmacoscintigraphic and aqueous humoral drug availability of ganciclovir-loaded mucoadhesive nanoparticles in rabbits

2013 ◽  
Vol 5 (3) ◽  
Author(s):  
Sohail Akhter ◽  
Farshad Ramazani ◽  
Mohammad Zaki Ahmad ◽  
Farhan Jalees Ahmad ◽  
Ziyaur Rahman ◽  
...  
Keyword(s):  
Particle Size ◽  
Aqueous Humor ◽  
Residence Time ◽  
Present Report ◽  
Plga Nanoparticles ◽  
Drug Availability ◽  
Rabbit Eye ◽  
Chitosan Coating ◽  
Coated Nanoparticles ◽  
Ocular Retention

AbstractThe present report describes the improved ocular retention and aqueous humoral drug availability of ganciclovir (GCV) when administered via topical instillation of different kind of nanoparticles onto the rabbit eye. GCV was loaded into PLGA nanoparticles, chitosan-coated nanoparticles and chitosan-coated niosomal nanoparticles. All three formulations contained nanoparticles equally round in shape with a mean particle size in the range of 180–200 nm. The ocular corneal retention property was evaluated by gamma scintigraphy, revealing that the clearance was slowest in the case of the chitosan-containing formulations. GCV in chitosan-coated PLGA nanoparticles and chitosan-coated niosomal nanoparticles showed approx. 6-fold higher aqueous humor drug availability as compared to a GCV solution and nearly 2.5-fold higher as compared to the chitosan-lacking GCV-PLGA nanoparticles. The results indicate that the use of a mucoadhesive chitosan coating can improve the ocular residence time and aqueous humoral availability of GCV when administered topically in nanoparticles.

Cytotoxicity to cervical cancer cells of Fe3O4 magnetic nanoparticles coated with cholic acid

2020 ◽  
Vol 10 (9) ◽  
pp. 1567-1572
Author(s):  
Yurong Liu ◽  
Xiaoyan Hou ◽  
Lianwei Lu ◽  
Ruixiang Wang
Keyword(s):  
Cervical Cancer ◽  
Particle Size ◽  
Cancer Cells ◽  
Hela Cells ◽  
Drug Loading ◽  
Plga Nanoparticles ◽  
Mtt Method ◽  
Star Copolymers ◽  
Cervical Cancer Cells

This study examined the effect of nanosized ferric oxide (Fe3O4) particles coated with different materials on the toxicity to HeLa cervical cancer cells. Magnetic Fe3O4 nanoparticles were prepared using a solventless thermal decomposition method and coated with either PLGA or CA-PLGA star copolymers. The uptake of nanoparticles by HeLa cells was observed by laser confocal microscopy. The toxicity to HeLa cells of Fe3O4 nanoparticles coated with these two materials was determined by the thiazole blue (MTT) method. The particle size of the single Fe3O4 nanoparticles was about 7 nm, and the PLGA and CA-PLGA nanoparticles loaded with Fe3O4 were spherical, with a particle size of about 200 mm and a theoretical drug loading of 10%. When the mass concentration of Fe3O4 nanoparticles is the same (25 pg/mL), the toxicity of Fe3O4-loaded CA-PLGA nanoparticles to HeLa cells is less than that of the corresponding PLGA nanoparticles. Thus, the CA-PLGA star copolymer can reduce the cytotoxicity of magnetic Fe3O4 nanoparticles and offers potential for broad application in vivo.

scholarly journals Dual encapsulation of hydrophobic and hydrophilic drugs in PLGA nanoparticles by a single-step method: drug delivery and cytotoxicity assays

RSC Advances ◽  
2016 ◽  
Vol 6 (112) ◽  
pp. 111060-111069 ◽  
Author(s):  
Laura Español ◽  
Ane Larrea ◽  
Vanesa Andreu ◽  
Gracia Mendoza ◽  
Manuel Arruebo ◽  
...  
Keyword(s):  
Drug Delivery ◽  
Plga Nanoparticles ◽  
Single Step ◽  
Step Method ◽  
Drug Encapsulation ◽  
Hydrophilic Drugs ◽  
Biodegradable Nanoparticles ◽  
Cytotoxicity Assays ◽  
Dual Drug

Dual drug encapsulation in biodegradable nanoparticles is always challenging and often requires strenuous optimization of the synthesis–encapsulation processes.

scholarly journals Encapsulation of metronidazole in polycaprolactone microspheres

2019 ◽  
Vol 9 (1) ◽  
pp. 190-194
Author(s):  
Rima Kassab ◽  
Dima Moussa ◽  
Cherine Saliba ◽  
Paolo Yammine
Keyword(s):  
Particle Size ◽  
Drug Release ◽  
Drug Loading ◽  
Solvent Evaporation ◽  
Compatibility Study ◽  
Drug Encapsulation ◽  
Evaporation Technique ◽  
Ft Ir ◽  
Ir Study

Non-aqueous oil-in-oil solvent evaporation technique is used for the preparation of polycaprolactone microspheres loaded with the antibiotic metronidazole by introducing different masses for the drug. The prepared microspheres are characterized by calculating drug encapsulation and drug loading percentages, measuring the corresponding particle size, performing FT-IR polymer-drug compatibility study and in vitro drug release. Moderate drug encapsulation values with a maximum of 34% are observed due to the low molecular weight of the drug. Microspheres had a particle size ranging between 130 and 280 µm with a spherical profile and porous structure. FT-IR study showed no interactions between the drug and the polymer. Drug release studies showed fast release rates for all the formulations with the slowest release for the highest drug loading. Keywords: polycaprolactone, metronidazole, targeted drug delivery, solvent evaporation.

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