scholarly journals The structure of an acylated inositol mannoside in the lipids of propionic acid bacteria

1969 ◽  
Vol 112 (5) ◽  
pp. 769-775 ◽  
Author(s):  
N. Shaw ◽  
F. Dinglinger
Keyword(s):  
Mass Spectrometry ◽  
Fatty Acids ◽  
Cell Walls ◽  
Periodate Oxidation ◽  
Heptadecanoic Acid ◽  
Pentadecanoic Acid ◽  
Propionic Acid Bacteria ◽  
Branched Chain ◽  
Chloroform Methanol ◽  
Hydrolysis Of

1. Lipids were extracted from five strains of Propionibacterium with chloroform–methanol mixtures and fractionated by chromatography on silicic acid. 2. All five extracts contained a glycolipid composed of fatty acids, inositol and mannose in the molar proportions 2:1:1. 3. Hydrolysis of the glycolipid with alkali gave a mixture of fatty acids and O-α-d-mannopyranosyl-(1→2)-myoinositol. 4. Analysis of the fatty acids by g.l.c. showed that they were predominantly straight- and branched-chain isomers of pentadecanoic acid and heptadecanoic acid. 5. The location and distribution of the fatty acid residues in the molecule was established by periodate oxidation studies and mass spectrometry. The structure of the major glycolipid is 1-O-pentadecanoyl-2-O-(6-O-heptadecanoyl-α-d-mannopyranosyl)myoinositol. 6. The glycolipids are located in the membrane; the cell walls are devoid of lipid. 7. Possible functions of the glycolipid are discussed.

Cellular fatty acids of Peptococcus variabilis and Peptostreptococcus anaerobius

1977 ◽  
Vol 5 (6) ◽  
pp. 665-667
Author(s):  
C W Moss ◽  
M A Lambert ◽  
G L Lombard
Keyword(s):  
Mass Spectrometry ◽  
Fatty Acids ◽  
Gas Chromatography ◽  
Analytical Techniques ◽  
Gas Chromatography Mass Spectrometry ◽  
Cellular Fatty Acids ◽  
Chromatography Mass Spectrometry ◽  
Branched Chain

Cellular fatty acids of Peptococcus variables and Peptostreptococcus anaerobius were identified by gas chromatography, mass spectrometry, and associated analytical techniques. Iso- and anteiso-branched-chain acids were major components in both species.

The lipid composition of cashmere goat fleece

1995 ◽  
Vol 46 (6) ◽  
pp. 1259 ◽  
Author(s):  
GC Hillbrick ◽  
DJ Tucker ◽  
GC Smith
Keyword(s):  
Mass Spectrometry ◽  
Fatty Acids ◽  
Gas Chromatography ◽  
Fatty Acid Composition ◽  
Chemical Composition ◽  
Capra Hircus ◽  
Gas Chromatography Mass Spectrometry ◽  
Cashmere Goat ◽  
Cashmere Goats ◽  
Chloroform Methanol

This study examined the differences in the chemical composition of the lipid extracted from the fleeces of intact and gonadectomized Australian cashmere goats (Capra hircus laniger). Lipid was extracted with either petroleum ether or chloroform/methanol, and analysed by gas chromatography and gas chromatography/mass spectrometry. A large number of buck specific fatty acids (29), which could be potential pheromones, were identified or tentatively identified. The fatty acid composition of buck fleece lipid is more complex than previously reported.

The effect of growth environment on the chloroform–methanol and alkali-extractable cell wall and cytoplasm lipid levels of Mucor rouxii

1975 ◽  
Vol 21 (1) ◽  
pp. 79-84 ◽  
Author(s):  
S. Safe ◽  
J. Caldwell
Keyword(s):  
Fatty Acids ◽  
Cell Wall ◽  
Cell Walls ◽  
Lipid Levels ◽  
Mucor Rouxii ◽  
Growth Environment ◽  
Lipid Fractions ◽  
Filamentous Cell ◽  
The Individual ◽  
Chloroform Methanol

The distribution of chloroform–methanol and alkali-extractable lipids in the cell walls of aerobically grown filamentous cells from Mucor rouxii has been determined. The results have been compared with the corresponding lipid composition of yeast-like cells from M. rouxii, which can be produced in two ways: by growth under anaerobic conditions and by aerobic growth in the presence of 0.22% phenethyl alcohol (PEA). It was observed that in most cases the crude cytoplasmic fraction contained higher levels of several lipids (i.e., squalene, sterols, triterpenes, and fatty acids) than did the corresponding cell walls. The cell walls did, however, contain both "free" (chloroform–methanol extractable) and "bound" (alkali extractable) lipids although the relative amounts were markedly dependent on the cell growth environment. The aerobically grown filamentous cell walls contained higher levels of squalene, sterols, triterpenes, and fatty acids than did aerobically grown yeast-like PEA-induced cell walls and there was also considerable variation in the "free''/"bound" ratios of the various lipid components. The lipid levels in both the cell walls and cytoplasm of the anaerobically grown cells were considerably lower than those of the cells grown under aerobic conditions. In addition, the differences in the growth environment were also reflected in the compositions of the individual lipid fractions from both the cell wall and the cytoplasm fraction.

Production of new extracellular glycolipids by a strain ofCellulomonas cellulans(Oerskovia xanthineolytica) and their structural characterization

1998 ◽  
Vol 44 (3) ◽  
pp. 238-243 ◽  
Author(s):  
S Arino ◽  
R Marchal ◽  
J -P Vandecasteele
Keyword(s):  
Mass Spectrometry ◽  
Fatty Acids ◽  
Gas Chromatography ◽  
Cell Wall ◽  
Culture Medium ◽  
Soil Samples ◽  
Mass Spectrometry Analysis ◽  
Gas Chromatography Mass Spectrometry ◽  
Spectrometry Analysis ◽  
Hydrolysis Of

Glycolipid-producing bacteria were isolated from soil samples. One of the strains, identified as Cellulomonas cellulans (Oerskovia xanthineolytica), was found to produce significant amounts of unusual extracellular glycolipids, which were shown to be composed of at least 11 individual compounds. Hydrolysis of the glycolipid mixture and gas chromatography - mass spectrometry analysis revealed the presence of fatty acids and hydroxy fatty acids ranging from C10to C18, 16 of which were identified. The glycidic moiety consisted of glucose, rhamnose, and ribose. The same sugars were found to be present in the cell wall of Cellulomonas cellulans, which also contained polar lipids including glycolipids. During strain cultivation, glycolipid excretion was stimulated when nitrogen was exhausted from the culture medium. In these conditions, the production in fermenters on glycerol, expressed in glucose equivalents, reached 8.9 g/L. Cell hydrophobicity, which rose to 95% during the growth phase, decreased to 50% during the production phase. The overall results show that the bacterial cell wall is involved in the synthesis of these new extracellular glycolipids.Key words: glycolipid, excretion, Cellulomonas cellulans, Oerskovia xanthineolytica, cell wall.

scholarly journals The glycolipids of Lactobacillus casei A.T.C.C. 7469

1968 ◽  
Vol 107 (4) ◽  
pp. 491-496 ◽  
Author(s):  
N. Shaw ◽  
K. Heatherington ◽  
J Baddiley
Keyword(s):  
Fatty Acids ◽  
Lactobacillus Casei ◽  
Octadecenoic Acid ◽  
Deae Cellulose ◽  
Minor Component ◽  
Gas Liquid Chromatography ◽  
Chloroform Methanol ◽  
Hydrolysis Of ◽  
Lactobacillic Acid ◽  
Acetate Form

1. The lipids were extracted from Lactobacillus casei A.T.C.C. 7469 with chloroform–methanol mixtures. The glycolipids were obtained by chromatography on silicic acid and DEAE-cellulose (acetate form). 2. Hydrolysis of the glycolipids with alkali gave two glycerol glycosides and a mixture of fatty acids. 3. The glycosides were separated and their structures elucidated. The major component was O-α-d-galactopyranosyl-(1→2)-O-α-d-glucopyranosyl-(1→1)-glycerol and the minor component O-α-d-glucopyranosyl-(1→6)-O-α-d-galactopyranosyl-(1→2)-O-α-d-glucopyranosyl-(1→1)-glycerol. 4. Analysis of the fatty acids by gas–liquid chromatography showed that they were predominantly palmitic acid, octadecenoic acid and lactobacillic acid.

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