Molecular cloning, characterization and expression of a jasmonate biosynthetic pathway gene encoding allene oxide cyclase from Camptotheca acuminata

2008 ◽  
Vol 28 (6) ◽  
pp. 349-355 ◽  
Author(s):  
Yan Pi ◽  
Zhihua Liao ◽  
Keji Jiang ◽  
Beibei Huang ◽  
Zhongxiang Deng ◽  
...  
Keyword(s):  
Plant Species ◽  
Genomic Dna ◽  
Amino Acid Level ◽  
Camptotheca Acuminata ◽  
Pcr Analysis ◽  
Allene Oxide ◽  
Allene Oxide Cyclase ◽  
Significant Similarity ◽  
Real Time Quantitative Pcr ◽  
Pathway Gene

AOC (allene oxide cyclase; EC 5.3.99.6), an essential enzyme in jasmonic acid and its methyl ester biosynthesis, was cloned from Camptotheca acuminata (named as CaAOC), a native medicinal plant species in China. CaAOC had significant similarity at the amino-acid level with AOCs from other plant species. Comparison between the sequences of the full-length cDNA and genomic DNA of CaAOC revealed that the genomic DNA of CaAOC contained an 89-bp intron and a 240-bp intron. Southern-blot analysis indicated that CaAOC was a multiple-copy gene, and real-time quantitative PCR analysis showed that CaAOC was expressed constitutively in all organs tested, with the highest expression level in leaves. The results from treatment experiments using different signalling components, including methyl jasmonate, abscisic acid, salicylic acid and H2O2, revealed that expression of CaAOC had a prominent diversity. Heavy metal (copper) significantly enhanced CaAOC expression, whereas wounding (induced by UV-B) was not so effective.

Molecular cloning and expression of a jasmonate biosynthetic gene allene oxide cyclase from Camellia sinensis

2016 ◽  
Vol 96 (1) ◽  
pp. 109-116 ◽  
Author(s):  
Meng-xin Wang ◽  
Qing-ping Ma ◽  
Bao-yu Han ◽  
Xing-hui Li
Keyword(s):  
Camellia Sinensis ◽  
Defense Responses ◽  
Tea Plant ◽  
Cloning And Expression ◽  
Pcr Analysis ◽  
Allene Oxide ◽  
Coding Region ◽  
Allene Oxide Cyclase ◽  
Reading Frame ◽  
Real Time Quantitative Pcr

As a family of signaling plant hormone, jasmonic acid plays an important role in coordinating plant defense responses to pests and pathogen attack through transcriptional and metabolic changes. In the jasmonate biosynthetic pathway of plants, allene oxide cyclase (AOC) is an essential enzyme. Here we cloned a cDNA from tea plant (Camellia sinensis), named as CsAOC (GenBank: HQ889679), which was 916 bp, containing an open reading frame (738 bp) encoding 245 amino acids. Comparative and bioinformatic analyses revealed that the deduced protein of CsAOC was highly homologous to AOC from other plant species. Phylogenetic analysis indicated that CsAOC was clustered in a closely related subgroup with AOC of Ipomoea nil. The full-length coding region of CsAOC was ligated with pET-32a and successfully expressed in E. coli BL21 (DE3), and purified. Real-time quantitative PCR analysis revealed that methyl jasmonate (MeJA) treatment on its own potently enhanced its expression over the control (healthy leaves), suggesting feedback activation of CsAOC. The expression under salicylic acid (SA) and wounding treatments was up-regulated. The mRNA expression of CsAOC could be induced by tea geometrids and tea green leafhoppers.

scholarly journals Comparative Proteomic Analysis of Dipsacus asperoides Roots from Different Habitats in China

Molecules ◽  
2020 ◽  
Vol 25 (16) ◽  
pp. 3605
Author(s):  
Haijun Jin ◽  
Hua Yu ◽  
Haixia Wang ◽  
Jia Zhang
Keyword(s):  
Proteomic Analysis ◽  
Absolute Quantification ◽  
Pcr Analysis ◽  
Allene Oxide ◽  
Allene Oxide Cyclase ◽  
Protein Levels ◽  
Depth Analysis ◽  
Differential Proteins ◽  
Isobaric Tags

Dipsacus asperoides is a kind of Chinese herbal medicine with beneficial health properties. To date, the quality of D. asperoides from different habitats has shown significant differences. However, the molecular differences in D. asperoides from different habitats are still unknown. The aim of this study was to investigate the differences in protein levels of D. asperoides from different habitats. Isobaric tags for relative and absolute quantification (iTRAQ) and 2DLC/MS/MS were used to detect statistically significant changes in D. asperoides from different habitats. Through proteomic analysis, a total of 2149 proteins were identified, of which 42 important differentially expressed proteins were screened. Through in-depth analysis of differential proteins, the protein metabolism energy and carbohydrate metabolism of D. asperoides from Hubei Province were strong, but their antioxidant capacity was weak. We found that three proteins, UTP-glucose-1-phosphate uridylyltransferase, allene oxide cyclase, and isopentyl diphosphate isomerase 2, may be the key proteins involved in dipsacus saponin VI synthesis. Eight proteins were found in D. asperoides in response to environmental stress from different habitats. Quantitative real-time PCR analysis confirmed the accuracy and authenticity of the proteomic analysis. The results of this study may provide the basic information for exploring the cause of differences in secondary metabolites in different habitats of D. asperoides and the protein mechanism governing differences in quality.

scholarly journals Identification and Expression Analysis of Two allene oxide cyclase (AOC) Genes in Watermelon

Agriculture ◽  
2019 ◽  
Vol 9 (10) ◽  
pp. 225 ◽  
Author(s):  
Jingwen Li ◽  
Yelan Guang ◽  
Youxin Yang ◽  
Yong Zhou
Keyword(s):  
Functional Divergence ◽  
Expression Patterns ◽  
Red Light ◽  
Pcr Analysis ◽  
Infected Leaf ◽  
Allene Oxide ◽  
Primary Role ◽  
Root Knot Nematode ◽  
Allene Oxide Cyclase ◽  
Wide Range

Allene oxide cyclase (AOC, EC 5.3.99.6) catalyzes the most important step in the jasmonic acid (JA) biosynthetic pathway and mediates plant defense response to a wide range of biotic and abiotic stresses. In this study, two AOC genes were identified from watermelon. Sequence analysis revealed that each of ClAOC1 and ClAOC2 contained an allene oxide cyclase domain and comprised eight highly conserved β-strands, which are the typical characteristics of AOC proteins. Phylogenetic analysis showed that ClAOC1 and ClAOC2 were clustered together with AOCs from dicotyledon, with the closest relationships with JcAOC from Jatropha curcas and Ljaoc1 from Lotus japonicus. Different intron numbers were observed in ClAOC1 and ClAOC2, which may result in their functional divergence. qRT-PCR analysis revealed that ClAOC1 and ClAOC2 have specific and complex expression patterns in multiple organs and under hormone treatments. Both ClAOC1 and ClAOC2 displayed the highest transcriptional levels in stem apex and fruit and exhibited relatively lower expression in stem. JA, salicylic acid (SA), and ethylene (ET) could enhance the expression of ClAOC1 and ClAOC2, particularly that of ClAOC2. Red light could induce the expression of ClAOC2 in root-knot nematode infected leaf and root of watermelon, indicating that ClAOC2 might play a primary role in red light-induced resistance against root-knot nematodes through JA signal pathway. These findings provide important information for further research on AOC genes in watermelon.

Molecular cloning and characterization of a jasmonate biosynthetic pathway gene for allene oxide cyclase from Jatropha curcas

2010 ◽  
Vol 32 (3) ◽  
pp. 531-539 ◽  
Author(s):  
Bin Liu ◽  
Wenguo Wang ◽  
Jihai Gao ◽  
Fang Chen ◽  
Shenghua Wang ◽  
...  
Keyword(s):  
Molecular Cloning ◽  
Jatropha Curcas ◽  
Biosynthetic Pathway ◽  
Allene Oxide ◽  
Allene Oxide Cyclase ◽  
Pathway Gene

Allene Oxide Cyclase from Camptotheca acuminata Improves Tolerance Against Low Temperature and Salt Stress in Tobacco and Bacteria

2008 ◽  
Vol 41 (2) ◽  
pp. 115-122 ◽  
Author(s):  
Yan Pi ◽  
Keji Jiang ◽  
Ying Cao ◽  
Qian Wang ◽  
Zhuoshi Huang ◽  
...  
Keyword(s):  
Salt Stress ◽  
Low Temperature ◽  
Camptotheca Acuminata ◽  
Allene Oxide ◽  
Allene Oxide Cyclase

scholarly journals Parishin fromGastrodia elataExtends the Lifespan of Yeast via Regulation of Sir2/Uth1/TOR Signaling Pathway

2016 ◽  
Vol 2016 ◽  
pp. 1-11 ◽  
Author(s):  
Yanfei Lin ◽  
Yujuan Sun ◽  
Yufang Weng ◽  
Akira Matsuura ◽  
Lan Xiang ◽  
...  
Keyword(s):  
Oxidative Stress ◽  
Gene Expression ◽  
Ribosomal Protein ◽  
Signaling Pathway ◽  
Pcr Analysis ◽  
Control Group ◽  
Tor Signaling ◽  
Sod Activity ◽  
Real Time Quantitative Pcr ◽  
Pathway Gene

Parishin is a phenolic glucoside isolated fromGastrodia elata, which is an important traditional Chinese medicine; this glucoside significantly extended the replicative lifespan of K6001 yeast at 3, 10, and 30 μM. To clarify its mechanism of action, assessment of oxidative stress resistance, superoxide dismutase (SOD) activity, malondialdehyde (MDA), and reactive oxygen species (ROS) assays, replicative lifespans ofsod1,sod2,uth1, andskn7yeast mutants, and real-time quantitative PCR (RT-PCR) analysis were conducted. The significant increase of cell survival rate in oxidative stress condition was observed in parishin-treated groups. Silent information regulator 2 (Sir2) gene expression and SOD activity were significantly increased after treating parishin in normal condition. Meanwhile, the levels of ROS and MDA in yeast were significantly decreased. The replicative lifespans ofsod1,sod2,uth1,andskn7mutants of K6001 yeast were not affected by parishin. We also found that parishin could decrease the gene expression ofTORC1, ribosomal protein S26A (RPS26A), and ribosomal protein L9A (RPL9A) in the target of rapamycin (TOR) signaling pathway. Gene expression levels ofRPS26AandRPL9Ainuth1, as well as inuth1,sir2double mutants, were significantly lower than those of the control group. Besides,TORC1gene expression inuth1mutant of K6001 yeast was inhibited significantly. These results suggested that parishin exhibited antiaging effects via regulation of Sir2/Uth1/TOR signaling pathway.

scholarly journals Biodegradation of Chloroxylenol by Cunninghamella elegans IM 1785/21GP and Trametes versicolor IM 373: Insight into Ecotoxicity and Metabolic Pathways

2021 ◽  
Vol 22 (9) ◽  
pp. 4360
Author(s):  
Marta Nowak ◽  
Katarzyna Zawadzka ◽  
Janusz Szemraj ◽  
Aleksandra Góralczyk-Bińkowska ◽  
Katarzyna Lisowska
Keyword(s):  
Trametes Versicolor ◽  
Significant Rise ◽  
Pcr Analysis ◽  
Cunninghamella Elegans ◽  
Oxidation Reactions ◽  
Cytochrome P450 Enzymes ◽  
C Elegans ◽  
Real Time Quantitative Pcr ◽  
Genes Expression ◽  
By Products

Chloroxylenol (PCMX) is applied as a preservative and disinfectant in personal care products, currently recommended for use to inactivate the SARS-CoV-2 virus. Its intensive application leads to the release of PCMX into the environment, which can have a harmful impact on aquatic and soil biotas. The aim of this study was to assess the mechanism of chloroxylenol biodegradation by the fungal strains Cunninghamella elegans IM 1785/21GP and Trametes versicolor IM 373, and investigate the ecotoxicity of emerging by-products. The residues of PCMX and formed metabolites were analysed using GC-MS. The elimination of PCMX in the cultures of tested microorganisms was above 70%. Five fungal by-products were detected for the first time. Identified intermediates were performed by dechlorination, hydroxylation, and oxidation reactions catalysed by cytochrome P450 enzymes and laccase. A real-time quantitative PCR analysis confirmed an increase in CYP450 genes expression in C. elegans cells. In the case of T. versicolor, spectrophotometric measurement of the oxidation of 2,20-azino-bis (3-ethylbenzthiazoline-6-sulfonic acid) (ABTS) showed a significant rise in laccase activity during PCMX elimination. Furthermore, with the use of bioindicators from different ecosystems (Daphtoxkit F and Phytotoxkit), it was revealed that the biodegradation process of PCMX had a detoxifying nature.

scholarly journals Allene oxide cyclase dependence of the wound response and vascular bundle-specific generation of jasmonates in tomato - amplification in wound signalling

2003 ◽  
Vol 33 (3) ◽  
pp. 577-589 ◽  
Author(s):  
Irene Stenzel ◽  
Bettina Hause ◽  
Helmut Maucher ◽  
Andrea Pitzschke ◽  
Otto Miersch ◽  
...  
Keyword(s):  
Vascular Bundle ◽  
Wound Response ◽  
Allene Oxide ◽  
Allene Oxide Cyclase
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