Bacteriophage gene products as potential antimicrobials against tuberculosis

2019 ◽  
Vol 47 (3) ◽  
pp. 847-860 ◽  
Author(s):  
Maria Puiu ◽  
Christina Julius

Abstract Tuberculosis (TB) is recognised as one of the most pressing global health threats among infectious diseases. Bacteriophages are adapted for killing of their host, and they were exploited in antibacterial therapy already before the discovery of antibiotics. Antibiotics as broadly active drugs overshadowed phage therapy for a long time. However, owing to the rapid spread of antibiotic resistance and the increasing complexity of treatment of drug-resistant TB, mycobacteriophages are being studied for their antimicrobial potential. Besides phage therapy, which is the administration of live phages to infected patients, the development of drugs of phage origin is gaining interest. This path of medical research might provide us with a new pool of previously undiscovered inhibition mechanisms and molecular interactions which are also of interest in basic research of cellular processes, such as transcription. The current state of research on mycobacteriophage-derived anti-TB treatment is reviewed in comparison with inhibitors from other phages, and with focus on transcription as the host target process.

Muzealnictwo ◽  
2020 ◽  
Vol 61 ◽  
pp. 80-89
Author(s):  
Agnieszka Bender

The current state of research into the patronage and collecting activities of Prince Stanisław Poniatowski (1754–1833) in Italy with which he was connected for almost 40 years of his life is outlined. Over the period, the Prince made 3 long trips there which preceded 30 years of living in Italy on a permanent basis, first in Rome, and then in Florence where he was buried. Despite his many accomplishments and extraordinary personal history, the Prince has not taken as prominent a position either in academic research or in the collective memory of Poles as his junior cousin Prince Józef who drowned in the Elster River giving his life for the homeland. Although no monograph has as yet been published on Stanisław Poniatowski, he is not entirely forgotten. However, the major studies dedicated to him were published a relatively long time ago. The most extensive, i.e. the book I Poniatowski a Roma (Firenze 1972), speaking of the history of Prince Stanisław and the Poniatowski family, was written by the Italian writer and columnist Andrea Busiri Vici and has never been translated into Polish. As the current state of research shows, very few Polish art historians have taken any in depth interest in the Prince’s activity. Apart from the mentions scattered in different studies, there are merely four articles dealing with some selected aspects of the Prince’s patronage and collecting activity (by Janina Michałkowa, Elżbieta Budzińska, Tadeusz Jaroszewski, Dominika Wronikowska). Historically the most complete study dedicated to Prince Stanisław is to be found in Jerzy Michalski’s paper from 50 years ago published in the Polish Biographical Dictionary. The to-date Polish and foreign publications in history, history of art and archaeology do not exhaust many issues related to the person, activity, and the collecting passion of Prince Stanisław.


Author(s):  
Dariusz BOBAK ◽  
Marta POŁTOWICZ-BOBAK

The terrains of Poland, located north of the Carpathians and the Sudetes, have been almost completely abandoned during the period of the LGM sensustricte. The reoccupation of Polish territories took place not until the end of LGM. These areas were then settled by the societies of the Magdalenian complex – a tradition that included upland areas of Western and Central Europe. On the basis of today's state of knowledge, it can be concluded that the eastern borders of Poland are at the same time the eastern boundary of the Magdalenien settlement. Five Magdalenian sites from the areas of today's Podkarpackie Voivodship are known (fig. 1). In the 1940s, a single-row harpoon linked to Magdalenian was found in Przemyśl. Further discoveries of sites fall into the turn of the 20th and 21st centuries. Four of the mhave been discovered up to this day: in Hłomcza, Grodzisko Dolne, Wierzawice and Łąka. This sites are only short-lived campsprovided small inventories. So far, no traces of large, longer settled base camp types have been found. If this situation is not only the result of the current state of research, then it may suggest that the areas of south-eastern Poland were part of a larger territory exploited by some Magdalenian community. On the basis of the analysis, it may be assumed that this territory may have covered the areas of eastern Poland. Magdalenian settlement in eastern Poland continues throughout the presence of Late Magdalenian societies in Central Europe, from Dryas I to Alleröd. It means that the population, or traditions of this culture, reached the eastern periphery relatively quickly and for a long time. The question arises if the Magdalenian population, functioning in the eastern borderlands, occupied areas not covered by any previous settlement, and whether were there contacts between them and representatives of other traditions – Epigravettian, whose settlement extends east of today's Polish borders on the territory of Ukraine. Finally, the last question is whether the line of the San is the final eastern limit of Magdalenian. This last question should be answered in the affirmatively, though not categorically. So far, we do not know of any Magdalenian sites from the areas east of Poland. The answer to the remaining questions is difficult. A certain light is being shed on them by the discovery of the site in Święte. The part of the site studied so far provided a small concentration of lithic artefacts – flakes and blades as well as several tools. These materials were described as Epigravettian. The TL dates obtained from the profile indicate that it is contemporary to the Magdalenian settlement. Perhaps, therefore the Magdalenian population who came to this area inhabited the areas that were occupied by the “Epigravettian” population? Perhaps we are also dealing with a zone penetrated by both these communities? So far, we know only one Epigravettian site from this area, which is contemporary to the Magdalenian settlement, but its significance in the discussion of Magdalenien-Epigravettian relations is very important. To what extent this borderland was the area of contacts and what the consequences could have been is unexplained yet. Apart from the few possible imports of Volhynian flint in Magdalenian inventories (Wierzawice, Grodzisko Dolne?), there are no other elements that could be a material confirmation of such contacts. An in-depth analysis of possible contacts on the west-east axis is also hindered by the poor level of recognition of the Polish-Ukrainian borderland on the Ukrainian side. Research conducted in the south-east of Poland shows that the Polish-Ukrainian borderland is an important area through which the border between two cultural traditions passes at the beginning of the Late Pleistocene. This is an extremely important area in discussion on the relationship between Magdalenian and Epigravettian. Today's knowledge and questions set the prospects for further work. Key words: Epigravettian, Magdalenian, Upper Paleolithic, South-Eastern Poland, Polish-Ukrainian borderland, cultural contacts, imports.


1996 ◽  
pp. 441-446
Author(s):  
Arthur Green

This chapter looks at some old and new questions on early hasidism. In reopening the two great questions—hasidism's origins and its success—contemporary scholarship has negated almost all the once clearly established answers. One can no longer say that hasidism began because of persecution, especially not that it arose in reaction to the Chmielnicki massacre a century earlier or its long aftermath, as was once widely claimed. Nor can one say that hasidism was primarily or necessarily a reaction to Sabbateanism. What, then, is left by way of explanation? The chapter then considers four questions about the beginning and success of hasidism. How and why did hasidism begin? What was the secret of its great success and rapid spread? What, if anything, is new in hasidism? How are the parameters of the movement to be defined—who is and who is not a hasid? From the perspective of the current state of research, the chapter answers each of these questions, identifying the present stand.


2020 ◽  
Vol 2 ◽  
pp. 145-158
Author(s):  
Kenta Nagasawa

Purpose: This paper is a thematic literature review to examine the current state of research about Culturally Responsive Pedagogy in mathematics. The main themes are students’ perception, teacher education for pre-service teacher and professional development for teachers. Research methods/ approach: Literature was collected from Eric, which is a research engine of the education field. Also, Google Scholar is used to find articles of major scholars introduced by Dr. Rich Milner, who is the instructor of this course. Findings: Students faced microaggressions in mathematics class, which discouraged them to learn mathematics. The effect of teacher education was inconsistent in terms of the awareness of culturally responsive pedagogy and lesson plans. Research of professional development mentioned that mathematics was cultural. Implications for research and practice: It is more interesting to conduct long term or follow-up research to find the teacher’s practice after a taking professional development program. Also, it is critical to expand research scope besides African American and Latino students. Finally, evidence-based research is needed to change the political situation. Keywords: culturally responsive teaching, mathematics, teacher education, professional development, student’s perception


2013 ◽  
Vol 1 (2) ◽  
pp. 1-3
Author(s):  
João José Pinto Ferreira ◽  
Anne-Laure Mention ◽  
Marko Torkkeli

The expansion of human knowledge in all areas is largely the outcome of the activity of academic institutions and the result of their mission to contribute to the cultural, intellectual and economic development of the society, involving education, research and university extension activities. For many years, the academic community has been organizing itself in all different ways to respond to current and future needs, ensuring research integrity and recognition, and building on successive generations of peers to validate and support the launching and development of novel research streams. We owe the current state of research and development of our society to generations of scholars and scientists that have brought all of us here.(...)


2020 ◽  
Author(s):  
Mariya Andriyanova ◽  
Aslanli Aslanli ◽  
Nataliya Basova ◽  
Viktor Bykov ◽  
Sergey Varfolomeev ◽  
...  

The collective monograph is devoted to discussing the history of creation, studying the properties, neutralizing and using organophosphorus neurotoxins, which include chemical warfare agents, agricultural crop protection chemical agents (herbicides and insecticides) and medicines. The monograph summarizes the results of current scientific research and new prospects for the development of this field of knowledge in the 21st century, including the use of modern physicochemical methods for experimental study and theoretical analysis of biocatalysis and its mechanisms based on molecular modeling with supercomputer power. The book is intended for specialists who are interested in the current state of research in the field of organophosphorus neurotoxins. The monograph will be useful for students, graduate students, researchers specializing in the field of physical chemistry, physicochemical biology, chemical enzymology, toxicology, biochemistry, molecular biology and genetics, biotechnology, nanotechnology and biomedicine.


i-Perception ◽  
2021 ◽  
Vol 12 (3) ◽  
pp. 204166952110203
Author(s):  
Jonas K. Olofsson ◽  
Ingrid Ekström ◽  
Maria Larsson ◽  
Steven Nordin

Olfaction, the sense of smell, is characterized by a notable age-dependency such that aging individuals are more likely to have poor olfactory abilities. These impairments are considered to be mostly irreversible and as having potentially profound effects on quality of life and food behavior, as well as constituting warning signs of mortality, cognitive dysfunction, and dementia. Here, we review the current state of research on aging and olfaction, focusing on five topics which we regard to be of particular relevance for the field: nutrition and health, cognition and dementia, mortality, environment and genetics, and training-based enhancement. Under each of these headlines, we provide a state-of-the-art overview and discuss gaps in our knowledge which might be filled by further research. Understanding how olfactory abilities are diminished in aging, and how they may be alleviated or recovered, involves a set of challenging tasks for researchers in the years to come.


2020 ◽  
Vol 7 (Supplement_1) ◽  
pp. S801-S801
Author(s):  
Jose Alexander ◽  
Daniel Navas ◽  
Marly Flowers ◽  
Angela Charles ◽  
Amy Carr

Abstract Background With the rise of the antimicrobial resistance between different genera and species of bacteria, Phage Therapy is becoming a more realistic and accessible option for patients with limited or no antimicrobial options. Being able to have rapid access to a collection of clinical active phages is key for rapid implementation of phage therapy. The Microbiology Department at AdventHealth Orlando is performing routine screening of environmental and patient samples for isolation of phages against non-fermenting Gram negative bacteria to develop a Phage Bank. Methods Protocols for phage isolation from environmental sources such as lakes, rivers and sewers and clinical samples were developed. A series of respiratory, throat, stool and urine samples were processed following an internal protocol that includes centrifugation, filtration and enrichment. Clinical samples were centrifugated for 10 minutes, filtered using 0.45µm centrifugation filters, seeded with targeted host bacteria (clinical isolates) and incubated at 35°C for 24 hours. The enriched samples were centrifugated and filtered for a final phage enriched solution. Screening and isolation were performed using the Gracia method over trypticase soybean agar (TSA) for plaque morphology and quantification. Host range screening of other clinical isolates of P. aeruginosa was performed using the new isolated and purified phages. Results 4 lytic phages against clinical strains of P. aeruginosa from patient with diagnosis of cystic fibrosis (CF), were isolated and purified from 4 different respiratory samples, including sputum and bronchial alveolar lavage. All phages showed phenotypical characteristics of lytic activity. 1 phage was active against 4 strains of P. aeruginosa, 1 phage was active against 2 strains of P. aeruginosa and the remaining 2 phages were active only against the initial host target strain. Conclusion With this study we demonstrated the potential use of clinical samples as source for isolating active bacteriophages against clinically significant bacteria strains. Clinical samples from vulnerable population of patients with chronic infections are part of our routine “phage-hunting” process to stock and grow our Phage Bank project for future clinical use. Disclosures All Authors: No reported disclosures


2021 ◽  
pp. 004728162110315
Author(s):  
Mason Pellegrini

Fierce competition has made innovation increasingly necessary for business success, and this has increased the importance of user-based innovation strategies like design thinking (DT). While many studies in technical and professional communication (TPC) have explored how DT can be used pedagogically, no studies have done this through investigating how DT is used as a workplace composing process. This study does exactly that. First, it presents the current state of research on pedagogical uses of DT in TPC, and then it builds upon those suggestions with an empirical study that chronicles on how two web design firms use DT to make websites. My main suggestion is to teach DT as a recursive process that allows students transcend potentially incorrect assumptions built into design tasks through gathering data not only from users, but from clients as well.


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