scholarly journals Comparison of the Phenolic Complex of Crimean Autochthonous and Classic White- Berry Grape Cultivars

2020 ◽  
Vol 161 ◽  
pp. 01059
Author(s):  
Elena Ostroukhova ◽  
Svetlana Levchenko ◽  
Irina Vasylyk ◽  
Vladimir Volynkin ◽  
Natalia Lutkova ◽  
...  
Keyword(s):  
Phenolic Compounds ◽  
Total Content ◽  
Total Phenolic ◽  
Hierarchical Analysis ◽  
Sauvignon Blanc ◽  
Ripening Period ◽  
Initial Values ◽  
Phenolic Components ◽  
Grape Cultivars

The profile of the phenolic components and features at the beginning of ripening (12.0 - 14.5 °Bx) and at the end of the observation (20.0-22.0 °Bx) of Crimean autochthonous white-berry grape cultivars are studied. The total content of identified phenolic compounds at the beginning of ripening differed depending on the cultivar from 669 mg kg-1 (Sauvignon Blanc) to 2411 mg kg-1 (Kokur Belyi). During the ripening period the content of components in berries of autochthonous grape cultivars decreased on average by 3.5 times of initial values (Kokur Belyi – by 4.1 times). In classic cultivars, this parameter did not change. The lowest concentration of phenolic components was observed in Shabash (256 mg kg-1) at 20.0-22.0 °Bx, the highest - in Riesling (1006 mg kg-1) and Chardonnay (827 mg kg-1). The hierarchical analysis of a cluster on a subject of total phenolic components revealed the similarity of autochthone Kokur Belyi to classic cultivars Chardonnay and Sauvignon Blanc. Autochthonous grape cultivars Sary Pandas and Shabash are closely related and form a separate cluster.

scholarly journals Relationships Between the Content of Phenolic Compounds and the Antioxidant Activity of Polish Honey Varieties as a Tool for Botanical Discrimination

Molecules ◽  
2021 ◽  
Vol 26 (6) ◽  
pp. 1810
Author(s):  
Monika Kędzierska-Matysek ◽  
Małgorzata Stryjecka ◽  
Anna Teter ◽  
Piotr Skałecki ◽  
Piotr Domaradzki ◽  
...  
Keyword(s):  
Principal Component Analysis ◽  
Antioxidant Activity ◽  
Phenolic Compounds ◽  
Caffeic Acid ◽  
Principal Component ◽  
Total Content ◽  
Component Analysis ◽  
Total Phenolic ◽  
Hydroxybenzoic Acid ◽  
Coumaric Acid

The study compared the content of eight phenolic acids and four flavonoids and the antioxidant activity of six Polish varietal honeys. An attempt was also made to determine the correlations between the antioxidant parameters of the honeys and their polyphenol profile using principal component analysis. Total phenolic content (TPC), total flavonoid content (TFC), antioxidant activity (ABTS) and reduction capacity (FRAP) were determined spectrophotometrically, and the phenolic compounds were determined using high-performance liquid chromatography (HPLC). The buckwheat honeys showed the strongest antioxidant activity, most likely because they had the highest concentrations of total phenols, total flavonoids, p-hydroxybenzoic acid, caffeic acid, p-coumaric acid, vanillic acid and chrysin. The principal component analysis (PCA) of the data showed significant relationships between the botanic origin of the honey, the total content of phenolic compounds and flavonoids and the antioxidant activity of the six Polish varietal honeys. The strongest, significant correlations were shown for parameters of antioxidant activity and TPC, TFC, p-hydroxybenzoic acid, caffeic acid and p-coumaric acid. Analysis of four principal components (explaining 86.9% of the total variance), as a classification tool, confirmed the distinctiveness of the Polish honeys in terms of their antioxidant activity and content of phenolic compounds.

scholarly journals Effect of physicochemical properties and phenolic compounds of bifloral propolis on antioxidant and antimicrobial capacity

Nova Scientia ◽  
2020 ◽  
Vol 12 (24) ◽  
Author(s):  
Rey David Vargas Sánchez ◽  
Evelin Martínez Benavidez ◽  
Javier Hernández ◽  
Gastón Ramón Torrescano Urrutia ◽  
Armida Sánchez Escalante
Keyword(s):  
Antioxidant Activity ◽  
Antibacterial Activity ◽  
Phenolic Compounds ◽  
Physicochemical Properties ◽  
Phenolic Content ◽  
Biological Activities ◽  
Sensory Characteristics ◽  
Total Phenolic ◽  
Gram Positive ◽  
Phenolic Components

In this study the effect of pollen source (mesquite and catclaw) on the sensory characteristics (appearance, color, aroma, taste, consistency and visible impurities), and physicochemical properties of raw propolis, and the phenolic content and biological activities of propolis extracts (PEs) was determined. The phenolic composition of PEs was determined by the total phenolic (TPC), flavone and flavonol (FFC), and flavanone and dihydroflavonol content (FDC). The individual phenolic components were analyzed by HPLC-DAD. The antioxidant activity was determined by the ferric-reducing power (FRAP) and free-radical scavenging activity (FRS). The antibacterial activity was evaluated against Gram-positive (Staphylococcus aureus and Listeria innocua) and Gram-negative (Echerichia coli and Salmonella thyphimurium) bacteria. The results showed that sensory characteristic and physicochemical properties of mesquite and catclaw propolis complied with international quality regulations. Fifteen phenolic compounds were identified, of which pinocembrin, naringenin, galangin, chrysin and quercetin were found a high concentration (> 3 mg/g). Mesquite propolis had the highest phenolic content (TFC and FDC), as well as antioxidant activity (> 2.5 mg Fe (II) equivalent/g; > 40% of DPPH radical inhibition) and antibacterial activity against Gram-positive bacterias in the order S. aureus > L. innocua (> 50% of inhibition for both bacterias at 500 µg/mL). These results indicating that pollen source affect the sensory characteristics and physicochemical properties of propolis, as well as the biological activity of their extracts.

scholarly journals Effect of Artificial Shading and Temperature on Radical Scavenging Activity and Polyphenolic Composition in Sweetpotato (Ipomoea batatas L.) Leaves

2003 ◽  
Vol 128 (2) ◽  
pp. 182-187 ◽  
Author(s):  
Md. Shahidul Islam ◽  
Makoto Yoshimoto ◽  
Koji Ishiguro ◽  
Shigenori Okuno ◽  
Osamu Yamakawa
Keyword(s):  
Phenolic Compounds ◽  
Ipomoea Batatas ◽  
Phenolic Content ◽  
Radical Scavenging Activity ◽  
Radical Scavenging ◽  
Cultivar Differences ◽  
Total Phenolic ◽  
Scavenging Activity ◽  
Caffeoylquinic Acid ◽  
Phenolic Components

The phenolic content and the radical scavenging activity were compared in leaves of sweetpotato (Ipomoea batatas L.) cultivars Shimon-1, Kyushu-119 and Elegant Summer grown under different temperature and shading conditions. Compared to cultivar differences, there was less effect of temperature and shading on the total phenolic content in sweetpotato leaves, however certain polyphenolic components differed widely among the treatments. The positive correlation between the radical scavenging activity and the level of total phenolics (r = 0.62) suggests that phenolic compounds are important antioxidant components of sweetpotato leaves. All the reverse-phase high-performance liquid chromatography (RP-HPLC) profiles of the cultivars tested showed peaks at the same retention times but peak areas of individual phenolic compounds differed with respective temperature and shading treatments. The phenolic compounds identified in the sweetpotato leaf were caffeic acid, chlorogenic acid, 4,5-di-O-caffeoylquinic acid, 3,5-di-O-caffeoylquinic acid, 3,4-di-O-caffeoylquinic acid, and 3,4,5-tri-O-caffeoylquinic acid. Most of the phenolic compounds were highest in leaves from plants grown at 20 °C without shading except 4,5-di-O-caffeoylquinic acid. The results indicate that growing leaves under moderately high temperatures and in full sun enhances the accumulation of phenolic components. These phenolic components have possible value in enhancing human health.

scholarly journals Ferulic and coumaric acids, total phenolic compounds and their correlation in selected oat genotypes

2008 ◽  
Vol 25 (No. 6) ◽  
pp. 325-332 ◽  
Author(s):  
M. Kováčová ◽  
E. Malinová
Keyword(s):  
Phenolic Compounds ◽  
Ferulic Acid ◽  
Functional Foods ◽  
Slovak Republic ◽  
Total Content ◽  
Climatic Conditions ◽  
Plant Production ◽  
Total Phenolic ◽  
Coumaric Acid ◽  
Total Phenolic Compounds

Ferulic and coumaric acids were determined by high-performance liquid chromatography in 21 oat genotypes supplied from the gene bank of the Research Institute of Plant Production in Piešťany (Slovak Republic) with the aim of selecting some genotypes suitable for the preparation of functional foods. The content of coumaric acid was higher than that of ferulic acid in 61.9% of samples. The latter acid exceeded proportionally the former one in genotypes with a low content of phenolic acids, namely in the amount of up to 50 mg/100 g of grain, especially in chaffless oats. Ferulic acid content varied in the range from 16.50 mg/100 g (cultivar Jakub) to 149.36 mg/100 g of grain (cultivar Izak). The content of coumaric acid ranged between 8.05 mg/100 g (cultivar Detvan) and 210.27 mg/100 g of grain (cultivar Izak). The fact that the samples were grown in the same year (2003) in one locality (Vígľaš-Pstruša, Slovak Republic) minimises the influence of soil and climatic conditions and proves that remarkable differences between the content of ferulic and coumaric acids and the total content of phenolic compounds were caused by the genetic outfit of oats. A high correlation was found between the contents of the total phenolic compounds, ferulic acid, coumaric acid and their sum. The corresponding correlation coefficients R had the values of 0.9229, 0.9141, and 0.9211, respectively. This correlation enables one to employ a simple and inexpensive method for the determination of the total phenolic compounds using Folin-Ciocalteu reagent for a rapid assessment of differences detectable in the content of the sum of ferulic and coumaric acids in oat samples in view of the selection of genotypes suitable for the preparation of functional foods.

scholarly journals Flavonoids and Other Phenolic Compounds in Needles of Pinus peuce and Other Pine Species from the Macedonian Flora

2015 ◽  
Vol 10 (6) ◽  
pp. 1934578X1501000 ◽  
Author(s):  
Marija Karapandzova ◽  
Gjose Stefkov ◽  
Ivana Cvetkovikj ◽  
Jasmina Petreska Stanoeva ◽  
Marina Stefova ◽  
...  
Keyword(s):  
Phenolic Compounds ◽  
Free Form ◽  
Flavonoid Glycosides ◽  
Total Phenolic ◽  
Total Flavonoids ◽  
Coumaric Acid ◽  
Phenolic Components ◽  
Total Flavonoids Content ◽  
Pinus Peuce ◽  
Pine Species

Flavonoids and other phenolic compounds in young needles of four pine species, Pinus peuce, P. nigra, P. mugo and P. sylvestris from the Macedonian flora were investigated. The amount of total phenols and total flavonoids were determined using Folin-Ciocalteau and aluminum chloride assay, respectively. The obtained results revealed that the total phenolic content (TPC) and total flavonoids content (TFC) varied among different pine species ranging from 9.8 to 14.0 mg GAE/g and from 3.3 to 7.2 mg CE/g of dried plant material, respectively. Qualitative analysis of flavonoids and other phenolic components was made by a LC-DAD/ESI-MSn optimized chromatographic method. A total of 17 phenolic components were identified and classified as: acids (2), procyanidins (2) and flavonoid glycosides (13). The most prevalent components were flavonoid glycosides, especially flavonols and methylated flavonols (9). Additionally, 3 components were found as acylated flavonol glycosides with ferulic and p-coumaric acid. The last one was found not only in esterified form but also in the free form. Only one flavone-apigenin glycoside was detected. Procyanidins were identified as catechin derivatives, both dimers and trimers.

scholarly journals Contents of dry matter, soluble sol­ids, vitamin C and phenolic compounds in the pulp and peel of different cultivars of pear fruits

2021 ◽  
Vol 28 (2) ◽  
Author(s):  
Jaunė Blažytė ◽  
Nijolė Vaitkevičienė
Keyword(s):  
Phenolic Compounds ◽  
Vitamin C ◽  
Dry Matter ◽  
Total Content ◽  
Biologically Active ◽  
Soluble Solids ◽  
Total Phenolic ◽  
Total Phenolic Compounds ◽  
Pear Fruit ◽  
Fruit Peel

The aim of this work was to investigate and compare the amounts of biologically active compounds, dry matter and soluble solids in pulp and peels of four pear cultivars (‘Ksena’, ‘Beloruskaja pozdniaja’, ‘Alna’ and ‘Aleksandr Lucas’). The research was carried out at Vytautas Magnus University Agriculture Academy (Lithuania) in 2020. Four pear cultivars (‘Ksena’, ‘Beloruskaja pozdniaja’, ‘Alna’ and ‘Aleksandr Lucas’) were cultivated at a farm in the Joniškis District of Lithuania. Pears were grown following traditional pear production technology. The amounts of dry matter, soluble solids, vitamin C and fiber were determined using the standard method. The total content of phenolic compounds was determined using a Folin-Ciocalteu reagent with a UV/VIS spectrophotometer. The research results showed that significantly the highest amounts of soluble solids were found in the ‘Alna’ pear fruit pulp (14.25% f. w.). The ‘Alna’ peel sample had significantly the highest amount of total phenolic compounds (1290.99 mg 100 g–1 d. w.). The highest content of dry matter was found in the ‘Aleksandr Lucas’ pear fruit peel (24.67%). The ‘Aleksandr Lucas’ and ‘Beloruskaja pozdniaja’ pear fruit peel contained significantly the highest amounts of vitamin C (2.70 and 2.63 mg 100 g–1 f. w., respectively). The highest content of fiber was found in the ‘Ksena’ fruit peel (36.12% d. w.). The investigated pear peel sample had significantly higher amounts of dry matter, vitamin C, total phenolic compounds and fiber than the pulp. However, the pulp contains the highest contents of soluble solids.

An investigation on Trakya region Oak (Quercus spp.) honeys of Turkey: their physico-chemical, antioxidant and phenolic compounds properties

2018 ◽  
Vol 43 (4) ◽  
pp. 362-374 ◽  
Author(s):  
Sevgi Kolayli ◽  
Zehra Can ◽  
Hilal Ebru Çakir ◽  
Onur Tolga Okan ◽  
Oktay Yildiz
Keyword(s):  
Phenolic Compounds ◽  
Physicochemical Properties ◽  
Optical Rotation ◽  
Radical Scavenging ◽  
High Ratio ◽  
Total Phenolic ◽  
Mineral Contents ◽  
Antioxidant Power ◽  
Phenolic Components ◽  
Ferric Reducing Antioxidant Power

Abstract Objective The purpose of this study was therefore to investigate various physicochemical properties, phenolic composition and antioxidant capacities of the oak honey, large quantities of which are produced in the Thrace region of Northwest Turkey. Methods Hunter color values (Lab), optical rotation, electrical conductivity, moisture and ash were measured as physicochemical determinants. Sugar, proline content, mineral, phenolic acids, total phenolic flavonoids contents were evaluated as chemical parameters. Ferric reducing antioxidant power (FRAP) and 2,2-diphenyl-1-picrylhydrazyl (DPPH) free radical scavenging were used as antioxidant determinants. Results The results determined that dark-colored honeys (mean 24.95±8.35 L) have proline levels (649.82±203.90 mg/kg), total phenolic content (TPC) (67.29±13.10 mgGAE/100 g) and total flavonoid content (TFC) (10.14±4.78 mgQE/100 g), a high ratio of fructose/glucose (1.65±0.18). Rutin (11.14±8.50 μg/g), p-coumaric acid (6.97±5.80 μg/g) and protocatechuic acid (6.12±4.39 μg/g) were the major phenolic components, and quercetin was also detected in varying amounts in all samples. Potassium (2523±0.28 mg/kg), calcium (63.40±3.20 mg/kg) and magnesium (38.88±0.65 mg/kg) were the major minerals in the honeys. Conclusion Our results showed that physicochemical properties, phenolic compounds, antioxidant capacity, sugar and mineral contents successfully distinguish the geographical origins of oak honey. These findings will contribute to a more accurate evaluation of oak honey in the literature.

scholarly journals TOTAL FENOL DAN AKTIVITAS ANTIOKSIDAN BUBUK KULIT MANGGIS (Garcinia mangostana L.) DARI BUAH SEGAR DENGAN VARIASI LAMA PENYIMPANAN YANG DIOLAH SECARA MEKANIS

2017 ◽  
Vol 21 (2) ◽  
pp. 110 ◽  
Author(s):  
Irwan Roza ◽  
Evawati Evawati ◽  
Rince Alfia Fadri ◽  
Gusmalini Gusmalini
Keyword(s):  
Antioxidant Activity ◽  
Phenolic Compounds ◽  
Phenolic Content ◽  
Total Content ◽  
Free Radical Scavengers ◽  
Total Phenolic ◽  
Radical Scavengers ◽  
Garcinia Mangostana ◽  
Storage Duration ◽  
Grinding Machine

This study aims to determine the total content of phenolic compounds and antioxidant activity of mangosteen skin powder from fresh fruit with variations of storage duration which processed mechanically. Methods of research done by determining the total content of phenolic compounds using the method of DPPH free radical scavengers. The fresh mangosteen skin was dried with a drying oven, with a capacity of 0.12 kg/h and a drying rate of 2.79 kg. Furthermore, dry mangosteen skin was milled by grinding machine with 2870 rpm rotation and obtained rendemen mangosteen skin powder as much as 38.04% with water content 8.33%. Total content of phenolic mangosteen powder with variation of storage duration 0 days, 10 days, and 20 days ranged 81.17 mgGAE / g - 195.51 mgGAE /g. The range of IC 50 for the three treatment is 6.80 ppm - 26.47 ppm. The highest total phenolic content and antioxidant activity werw found at zero days of storage.

scholarly journals THE COMPARATIVE STUDY OF ANTIOXIDANT PROPERTIES OF SOME SAMBUCUS L. SPECIES

2019 ◽  
pp. 127-137 ◽  
Author(s):  
Любовь (Lyubov') Николаевна (Nikolaevna) Скрыпник (Skrypnik) ◽  
Алина (Alina) Андреевна (Andreevna) Курашова (Kurashova)
Keyword(s):  
Antioxidant Activity ◽  
Phenolic Compounds ◽  
Antioxidant Properties ◽  
Total Content ◽  
Water Soluble ◽  
Biologically Active ◽  
Total Phenolic ◽  
Antioxidant Power ◽  
Sambucus Ebulus ◽  
Amperometric Method

The antioxidant properties of fruits, flowers, leaves, bark (or stem) of elderberry (Sambucus nigra L.), red elderberry (Sambucus racemosa L.) and dwarf elder (Sambucus ebulus L.) were investigated. The total content of anthocyanins and total phenolic compounds content by using of Folin-Ciocalteu assay were determined spectrophotometrically. The total content of water-soluble antioxidants was investigated by amperometric method. The antioxidant activity (AOA) of plant extracts was measured using DPPH (1,1-diphenyl-2-picrylhydrazyl) radical, ABTS (2,2’azinobis(3)ethylbenzthiazoline-6- sulfonic acid) radical and FRAP (ferric reducing antioxidant power) assays. It was established that the fruits of plants of the genus Sambucus L. were characterized by the maximum level of all studied antioxidants. High content of anthocyanins and phenolic compounds was determined in the leaves of elderberry and dwarf elder. The flowers of these elderberry species were distinguished by a high total content of water-soluble antioxidants. The highest antioxidant activity was observed in fruits extracts in comparison with other parts of the plant. Higher antioxidant activity was identified in the extract of the fruits of elderberry and dwarf elder than of the red elderberry fruits. The most optimal method for evaluating the antioxidant activity of elder extracts was the FRAP assay, which showed the highest correlation between AOA and individual antioxidant components, compared to DPPH and ABTS assays. Comparative analysis of antioxidant content and antioxidant activity of various plant parts of three elderberry species showed that the most promising sources of biologically active substances with antioxidant properties are fruits and flowers of elderberry and dwarf elder.

scholarly journals The influence of gamma-irradiation on the formation of free radicals and antioxidant status of oregano (Origanum vulgare L.)

2008 ◽  
Vol 25 (No. 3) ◽  
pp. 131-143 ◽  
Author(s):  
J. Horváthová ◽  
M. Suhaj ◽  
M. Polovka ◽  
V. Brezová ◽  
P. Šimko
Keyword(s):  
Thermal Stability ◽  
Antioxidant Activity ◽  
Phenolic Compounds ◽  
Epr Spectroscopy ◽  
Antioxidant Properties ◽  
Reducing Power ◽  
Total Content ◽  
Life Time ◽  
Total Phenolic ◽  
Origanum Vulgare

The influence of various gamma-radiation dose absorptions on oregano (<i>Origanum vulgare</i> L.) solid samples was monitored by means of electron paramagnetic resonance (EPR) spectroscopy. Further, the antioxidant activity of oregano methanol/water extracts was characterised using DPPH (1,1-diphenyl-2-picrylhydrazyl free radical); thiobarbituric acid reactive substances (TBARS); ferric reducing power (FRP); and total content of phenolic compounds (TPC) assays. EPR spectroscopy was used for the investigation of the influence of the absorbed dose on the character of the paramagnetic structures formed, as well as for their thermal stability and life-time characterisation. EPR spectrum of the reference (non-irradiated) sample represents a broad singlet line with unresolved hyperfine splitting, attributable to Mn(II) ions, upon which the additional sharp EPR signal (g = 2.0022, &Delta;B<sub>pp</sub> &asymp; 1 mT) is superimposed, assigned to stable semiquinone radicals produced by the oxidation of polyphenolic compounds present in plants. The additional paramagnetic structures of different origin (mostly of cellulose and carbohydrate), possessing diverse thermal stability and life-time, were identified in the gamma-irradiated samples. Immediately after irradiation, a statistically significant increase was observed of the TBARS values and the total content of phenolic compounds in methanol/water oregano extract. The alterations of the antioxidant properties of oregano extracts with the time after the radiation treatment were also monitored. A substantial time-dependent decrease of antioxidant activity was observed, probably as a result of storage, with both irradiated and non-irradiated oregano samples, as obvious from the ferric reducing power test and the content of total phenolic substances. The influence of irradiation and subsequent storage of oregano samples on the DPPH radical-scavenging ability was only negligible.

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