scholarly journals Comparative Study on Antioxidant Capacity of Self-fermenting Enzyme and Commercial Enzyme

2021 ◽  
Vol 251 ◽  
pp. 02032
Author(s):  
Fenghong Liu ◽  
Xianhao Cheng ◽  
Jing Miu ◽  
Xiaotong Cui ◽  
Xiaojuan Gao ◽  
...  
Keyword(s):  
Free Radicals ◽  
Comparative Study ◽  
Antioxidant Capacity ◽  
Hydroxyl Radicals ◽  
Superoxide Anion ◽  
Antioxidant Ability ◽  
Commercial Enzyme ◽  
Visible Spectrophotometry ◽  
Uv Visible ◽  
Living Bacteria

In order to provide consumers with a more reasonable method of using Enzyme, UV-visible spectrophotometry was used to study the antioxidant ability of self-fermenting Enzyme and commercial Enzyme. By measuring the ability of fourteen kinds of self-fermenting Enzyme and three commercial Enzyme to scavenge DPPH free radicals, superoxide anion and hydroxyl radicals and the number of living bacteria, the following conclusions were drawn: (1) Commercial Enzyme has more properties than self-fermenting Enzyme of antioxidant capacity; (2) Compound fruit Enzyme has stronger antioxidant ability than single fruit Enzyme; (3) Self-fermenting Enzyme is not sterilized including more living bacteria; (4) Commercial Enzyme has a higher drinking value than self-fermenting Enzyme.

Endothelial dysfunction in mesenteric resistance arteries of diabetic rats: role of free radicals

1994 ◽  
Vol 266 (3) ◽  
pp. H1153-H1161 ◽  
Author(s):  
D. Diederich ◽  
J. Skopec ◽  
A. Diederich ◽  
F. X. Dai
Keyword(s):  
Nitric Oxide ◽  
Superoxide Dismutase ◽  
Free Radicals ◽  
Endothelial Dysfunction ◽  
Hydroxyl Radicals ◽  
Superoxide Anion ◽  
Diabetic Rats ◽  
Resistance Arteries ◽  
Luminal Diameter

Diabetes was induced in rats by an injection of streptozotocin (55 mg/kg). Endothelium-dependent relaxations in mesenteric resistance arteries (luminal diameter 210 +/- 20 microns) of control and diabetic rats were compared in myographs. Acetylcholine induced endothelium-dependent relaxations that were mediated by nitric oxide (EDNO). EDNO-mediated relaxations were impaired in diabetic arteries; concentrations of acetylcholine required to produce 50% relaxation (ED50) of activated arteries were 5 nM in control and 13.5 nM in arteries from diabetic rats studied after 6 wk (P < 0.05). The impairment in relaxation worsened with duration of the diabetes; ED50 for acetylcholine increased to 63 and 100 nM in diabetic arteries studied after 16 and 24 wk of diabetes, respectively. NG-nitro-L-arginine produced 5.5- and 16-fold decreases in sensitivity of control and diabetic arteries to acetylcholine. NG-nitro-L-arginine produced at least as much inhibition of acetylcholine relaxations in diabetic arteries, indicating that the impaired relaxation noted in diabetic arteries does not result from decreased production of EDNO. EDNO-mediated relaxations in diabetic arteries were impaired by increased production of endothelium-derived free radicals. Superoxide dismutase, a scavenger of superoxide anion, and dimethylthiourea, a scavenger of hydroxyl radicals, normalized EDNO-mediated relaxations in diabetic arteries. The ED50 values for acetylcholine were 13.5, 5.5, and 4 nM for untreated and SOD- and DMTU-treated diabetic arteries, respectively (P < 0.05 for treated vs. untreated arteries). Superoxide anion and hydroxyl radicals appear to block EDNO-mediated relaxation by inactivating EDNO.(ABSTRACT TRUNCATED AT 250 WORDS)

scholarly journals Comparison of Antioxidant Activity of Ethanol Extract of Fruit and Okra Leaves (Abelmoschus esculentus L. Moench) with DPPH and ABTS Methods

2019 ◽  
Vol 2 (2) ◽  
pp. 6-13
Author(s):  
Hendri Faisal ◽  
Sri Handayani
Keyword(s):  
Antioxidant Activity ◽  
Free Radicals ◽  
Fruits And Vegetables ◽  
Ethanol Extract ◽  
Natural Antioxidants ◽  
Abelmoschus Esculentus ◽  
Visible Spectrophotometry ◽  
Dpph Method ◽  
Uv Visible ◽  
Free Radical Activity

Excessive free radical activity in the human body can be characterized by pathological conditions such as cardiovascular disease, pre-natal complications, arthritis, cataracts, parkinsonism, alzheimer's, and aging. One way to prevent the formation of free radicals is to use nutrients that act as antioxidants. Natural antioxidants found in fruits and vegetables are effective free radicals and are considered non-toxic when compared with synthetic antioxidants, one of which is the okra plant Abelmoschus esculentus L.The purpose of this study is to determine differences in the antioxidant activity ethanol extract of fruit  and okra leaves with DPPH method and ABTS method. The method used in this study is the maceration extraction method with 70% ethanol solvent to obtain fruit extracts and okra leaves. The antioxidant activity test was carried out by the DPPH (1,1-diphenyl-2-picrylhydrazi) method by measuring the absorption of a mixture of sample extracts and DPPH by UV-Visible spectrophotometry at a wavelength of 520 nm and by the ABTS method ((2,2-azinobis- (3 -Ethylbenzothiazoline-6-Sulfonic Acid) by measuring the absorption of a mixture of sample extracts and ABTS by UV-Visible spectrophotometry at a wavelength of 734 nm. measurement of antioxidant activity of okra fruit ethanol extract by ABTS method has very strong antioxidant activity (IC50 24.50 ppm)  

scholarly journals MALONDIALDEHYDE (MDA) (ZAT OKSIDAN YANG MEMPERCEPAT PROSES PENUAAN)

2020 ◽  
Vol 2 (2) ◽  
pp. 117-123
Author(s):  
Novrina Situmorang ◽  
Zulham Zulham
Keyword(s):  
Free Radicals ◽  
Hydroxyl Radicals ◽  
Superoxide Anion ◽  
Aging Process ◽  
Cell Damage ◽  
The Body ◽  
Premature Aging ◽  
Cell Components ◽  
Oxidation Damage ◽  
Superoxide Anion Radicals

Malondialdehyde (MDA) is the end product of fat peroxidation due to the breakdown of fatty acid chains which are compounds that toxic to cells. Fat peroxidation is caused by body fat bound to free radicals such as hydroxyl radicals, superoxide anion radicals, and hydrogen peroxide, which are compounds or atoms that have unpaired electrons in their outer orbitals so that they are very reactive to cells or surrounding cell components including lipids. As a result of the lipid peroxidation reaction which is continuous which can cause various diseases and accelerate the aging process, because malondialdehyde causes cell damage. The aging process is the weakening of cells and organs as a whole starting from adulthood slowly and progressing quickly after the age of 50 years, marked by the body begins to ache and wrinkled skin. Naturally, the aging process will occur in every human being, but the process is different, some are fast (the process of premature aging) and some are slow (ageless). Although the aging process occurs for several reasons, free radicals such as melondialdehyde also contribute to accelerating the aging process. Efforts to slow down premature aging due to free radicals, namely antioxidants. As an active ingredient, antioxidants are used to protect skin from oxidation damage and prevent premature aging.

Evaluation of Salivary Antioxidants and Oxidative Stress Markers in Male Smokers

2019 ◽  
Vol 22 (7) ◽  
pp. 496-501
Author(s):  
Fatemeh Ahmadi-Motamayel ◽  
Parisa Falsafi ◽  
Hamidreza Abolsamadi ◽  
Mohammad T. Goodarzi ◽  
Jalal Poorolajal
Keyword(s):  
Oxidative Stress ◽  
Uric Acid ◽  
Free Radicals ◽  
Antioxidant Capacity ◽  
Cigarette Smoke ◽  
Total Antioxidant Capacity ◽  
The Body ◽  
Total Antioxidant ◽  
The Mean ◽  
And Oxidative Stress

Background: Cigarette smoke free radicals can cause cellular damage and different diseases. All the body fluids have antioxidants which protect against free radicals. Objective: The aim of this study was to evaluate salivary total antioxidant capacity and peroxidase, uric acid and malondialdehyde levels in smokers and a nonsmoking control group. Methods: Unstimulated saliva was collected from 510 males. A total of 259 subjects were current smokers and 251 were non-smokers. The levels of salivary total antioxidant capacity, uric acid, peroxidase and malondialdehyde were measured using standard procedures. Data were analyzed with t test and ANOVA. Results: The smokers were younger and dental hygiene index was higher than healthy nonsmoking controls. The mean total antioxidant capacity in smokers and nonsmokers was 0.13±0.07 and 0.21±011, respectively (P=0.001). Smokers had significantly lower peroxidase and uric acid levels than healthy controls. In addition, the mean malondialdehyde levels in the smokers and nonsmokers were 4.55 ±2.61 and 2.79 ±2.21, respectively (P=0.001). Conclusion: Cigarette smoke produces free radical and oxidative stress, causing many side effects. Salivary antioxidant levels decreased and malondialdehyde levels increased in smokers, indicating the high oxidative stress among smokers compared to nonsmokers. Cigarette smoke had deleterious effects on main salivary antioxidants levels.

Shape and stability of silver nanoparticles and their dependence on the conditions of preparation.

2012 ◽  
Vol 1371 ◽  
Author(s):  
M.I. Hernández-Castillo ◽  
O. Zaca-Moran ◽  
P. Zaca-Moran ◽  
M. Rojas-López ◽  
V.L. Gayou ◽  
...  
Keyword(s):  
Silver Nanoparticles ◽  
Reaction Time ◽  
Ag Nanoparticles ◽  
Reduction Procedure ◽  
Visible Spectrophotometry ◽  
Logical Sequence ◽  
Uv Visible ◽  
Citrate Reduction ◽  
Shape And Size

ABSTRACTBy using the citrate reduction procedure we have synthesized Ag nanoparticles, applying several conditions of preparation, being after characterized by UV-visible spectrophotometry. Following a logical sequence, the starting experiment was realized varying the reaction time, after that it was varied the concentration of the reductor agent, and finally it was varied the volume of the reductor agent. According to this methodology, TEM measurements show that firstly we have nanostructures with different shape and size, whereas in the last part of the experiment we have Ag nanoparticles with homogeneous shape and size.

scholarly journals Isolasi dan Karakterisasi Bakteri Penghasil Etanol dari Lingkungan Industri Arak Bali di Desa Merita dan Tri Eka Buana, Karangasem-Bali

2020 ◽  
Vol 8 (2) ◽  
pp. 279
Author(s):  
Azis Akbar Hakim ◽  
I M. Mahaputra Wijaya ◽  
Ida Bagus Wayan Gunam
Keyword(s):  
Fermentation Process ◽  
Visible Spectrophotometry ◽  
Gram Positive Bacteria ◽  
Production Location ◽  
Coconut Tree ◽  
Isolation And Characterization ◽  
Exposure Method ◽  
Sucrose Medium ◽  
Uv Visible

The purpose of this research is isolation and characterization to find potential bacteria which can produce the most optimal ethanol from the Arak Bali industry in Karangasem Regency, Bali. Bacteria were isolated by exposure method in open air using selective media Zymomonas Sucrose Medium (ZSMA) with the addition of nystatin as much as 0.18 g / L as an antifungal then samples were taken at three different points in one Arak Bali production location, namely the distillation place, the fermentation room for roomie, and the place of taking coconut juice under the coconut tree and the variation of time is 15, 30, and 60 minutes of exposure. Gas checking is done on the bacteria obtained to select its ability to produce ethanol. The results of the scanning of 11 best isolates using UV-visible spectrophotometry were fermented on 500 mL ZSM media for 10 days. BM1-CP14 is the best isolate to produce total ethanol of 15.33 mL through the fermentation process. The results of the characterization of BM1-CP14 isolates were Gram-positive bacteria in the form of bacilli, anaerobic and non-motile bacteria. The results showed that bacteria isolated from open-air also can produce ethanol. Keyword: ethanol, Arak Bali, airborne bacterial exposure, isolation, characterization, UV-Visible spectrophotometry

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