scholarly journals Chemical composition, antioxidant and antimicrobial activities of the essential oil of Illicium verum

2021 ◽  
Vol 319 ◽  
pp. 01052
Author(s):  
Brahim Outemsaa ◽  
Asmaa Oubihi ◽  
Hassna Jaber ◽  
Sara Haida ◽  
Ikram Kenfaoui ◽  
...  

In recent years, a concern has been expressed about the impact of antibiotics and synthetic antioxidants, which are used to inhibit microbial growth and retard fat oxidation in foods. In addition, antibiotic resistance presents a serious menace to human and environmental ecosystems. This has led the food industry to use natural resources such as essential oils in the preparation of foods, this forming their sensory profile and increasing preservation time there. The objective of this work is to determine the chemical composition and evaluate the antibacterial and antioxidant activity of the essential oil of Illicium verum. The yield of essential oil extracted by hydro distillation is about 4.13%. The chemical composition of the essential oil extracted from the dried fruits of Illicium verum was studied by gas chromatography coupled with mass spectrometry (GC and GC/MS). Twenty-eight constituents, representing 99.74% of the essential oil were identified. The major compounds are: trans-anethole (83.46%), D-Limonene (4.56%), Estragole (3.47%) and Linalool (1.07%). Antioxidant activity was determined by the DPPH assay. The essential oils of Illicium verum showed low antioxidant activity with IC50= 286.19 ± 7.4 mg/mL, compared to Ascorbic acid IC50= 0.09 ± 0.01 mg/mL. The antibacterial effect of this essential oil was tested against six microorganisms, of which Staphylococcus aureus is the most sensitive with an MIC of about 1/1000 (v/v), followed by Staphylococcus epidermidis and Enterobacter cloacae with an MIC equal to 1/100 (v/v).

2020 ◽  
Vol 49 (4) ◽  
pp. 957-965
Author(s):  
Omer Elkiran ◽  
Cumhur Avsar

The chemical composition, antimicrobial and antioxidant properties of the essential oils from the leaves of endemic Thymus leucostomus naturally grown in Turkey were investigated and chemical differences were discussed by means of chemotaxonomy. Twenty-six components were identified representing 98.8% of the oils. The main compounds in the essential oil of T. leucostomus were: o-cymene (30.6%), carvacrol (9.6%), thymol methyl ether (7.2%), limonene (6.8%). Essential oil was screened for their antimicrobial activities against 7 bacteria and 2 yeast species by using disc-diffusion and MIC procedure. The essential oil showed higher effectiveness against all the tested bacteria and yeast. The extract was observed to be much more effective in Gram-positive bacteria (especially, S. aureus ATCC 6538). In vitro antioxidant activity based on the 1,1-diphenyl-2-picrylhydrazyl (DPPH) free radical was evaluated for the essential oil, and it was found that the essential oil had good antioxidant activity in the range of the IC50= 5.42 ±0.8 μg/ml.


Molecules ◽  
2021 ◽  
Vol 26 (10) ◽  
pp. 2888
Author(s):  
Carmen M. S. Ambrosio ◽  
Gloria L. Diaz-Arenas ◽  
Leidy P. A. Agudelo ◽  
Elena Stashenko ◽  
Carmen J. Contreras-Castillo ◽  
...  

Essential oils (EOs) from Citrus are the main by-product of Citrus-processing industries. In addition to food/beverage and cosmetic applications, citrus EOs could also potentially be used as an alternative to antibiotics in food-producing animals. A commercial citrus EO—Brazilian Orange Terpenes (BOT)—was fractionated by vacuum fractional distillation to separate BOT into various fractions: F1, F2, F3, and F4. Next, the chemical composition and biological activities of BOT and its fractions were characterized. Results showed the three first fractions had a high relative amount of limonene (≥10.86), even higher than the whole BOT. Conversely, F4 presented a larger relative amount of BOT’s minor compounds (carvone, cis-carveol, trans-carveol, cis-p-Mentha-2,8-dien-1-ol, and trans-p-Mentha-2,8-dien-1-ol) and a very low relative amount of limonene (0.08–0.13). Antibacterial activity results showed F4 was the only fraction exhibiting this activity, which was selective and higher activity on a pathogenic bacterium (E. coli) than on a beneficial bacterium (Lactobacillus sp.). However, F4 activity was lower than BOT. Similarly, F4 displayed the highest antioxidant activity among fractions (equivalent to BOT). These results indicated that probably those minor compounds that detected in F4 would be more involved in conferring the biological activities for this fraction and consequently for the whole BOT, instead of the major compound, limonene, playing this role exclusively.


2008 ◽  
Vol 3 (9) ◽  
pp. 1934578X0800300 ◽  
Author(s):  
Ana P. Murray ◽  
Silvana A. Rodriguez ◽  
María G. Murray

Schinus fasciculata and S. praecox (Anacardiaceae) can be found among the characteristic vegetation components of the phytogeographic province of the Espinal. The essential oils from these two species have been obtained from fresh material by hydrodistillation and analyzed by GC and GC-MS. We have compared the chemical composition of the oils obtained from samples collected at two different natural environments and at different phenological phases. The antioxidant activity of these oils was evaluated by the DPPH assay. S. fasciculata and S. praecox showed significant differences in their chemical composition as well as in their radical scavenger ability. The essential oil from samples of S. fasciculata attacked by plant-lice (Hemiptera, Psylloidea) was the more active one, with inhibition percentages higher than that of the reference compound.


2013 ◽  
Vol 8 (5) ◽  
pp. 504-511 ◽  
Author(s):  
Abdulhmid Giweli ◽  
Ana Džamić ◽  
Marina Soković ◽  
Mihailo Ristić ◽  
Petar Marin

AbstractThe composition of essential oil isolated from Thymus algeriensis growing wild in Libya was analyzed by GC and GC-MS. The essential oil was characterized with thymol (38.50%) as the major component. The oil was screened for antioxidant activity using DPPH assay, and compared to thymol and carvacrol. Antioxidant activity was high, with the IC50 of 0.299 mg/ml, compared to 0.403 and 0.105 mg/ml for thymol and carvacrol, and 0.0717 mg/ml for BHA. In addition, antimicrobial activity was tested against eight bacteria and eight fungi. T. algeriensis oil showed inhibitory activity against tested bacteria at 0.001–0.05 mg/ml, while bactericidal activity (MBC) was achieved at 0.0025–0.05 mg/ml. For antifungal activity MICs ranged 0.0005–0.025 mg/ml and MFC 0.001–0.05 mg/ml. High antimicrobial activity against the fungi in particular suggests that the essential oil of Thymus algeriensis could have a useful practical application.


Foods ◽  
2020 ◽  
Vol 9 (5) ◽  
pp. 550 ◽  
Author(s):  
Evelyne Amenan Tanoh ◽  
Guy Blanchard Boué ◽  
Fatimata Nea ◽  
Manon Genva ◽  
Esse Leon Wognin ◽  
...  

This study focused, for the first time, on the evaluation of the seasonal effect on the chemical composition and biological activities of essential oils hydrodistillated from leaves, trunk bark and fruits of Zanthoxylum leprieurii (Z. leprieurii), a traditional medicinal wild plant growing in Côte d’Ivoire. The essential oils were obtained by hydrodistillation from fresh organs of Z. leprieurii growing on the same site over several months using a Clevenger-type apparatus and analyzed by gas chromatography-mass spectrometry (GC/MS). Leaf essential oils were dominated by tridecan-2-one (9.00 ± 0.02–36.80 ± 0.06%), (E)-β-ocimene (1.30 ± 0.50–23.57 ± 0.47%), β-caryophyllene (7.00 ± 1.02–19.85 ± 0.48%), dendrolasin (1.79 ± 0.08–16.40 ± 0.85%) and undecan-2-one (1.20 ± 0.03–8.51 ± 0.35%). Fruit essential oils were rich in β-myrcene (16.40 ± 0.91–48.27 ± 0.26%), citronellol (1.90 ± 0.02–28.24 ± 0.10%) and geranial (5.30 ± 0.53–12.50 ± 0.47%). Tridecan-2-one (45.26 ± 0.96–78.80 ± 0.55%), β-caryophyllene (1.80 ± 0.23–13.20 ± 0.33%), α-humulene (4.30 ± 1.09–12.73 ± 1.41%) and tridecan-2-ol (2.23 ± 0.17–10.10 ± 0.61%) were identified as major components of trunk bark oils. Statistical analyses of essential oil compositions showed that the variability mainly comes from the organs. Indeed, principal component analysis (PCA) and hierarchical cluster analysis (HCA) allowed us to cluster the samples into three groups, each one consisting of one different Z. leprieurii organ, showing that essential oils hydrodistillated from the different organs do not display the same chemical composition. However, significant differences in essential oil compositions for the same organ were highlighted during the studied period, showing the impact of the seasonal effect on essential oil compositions. Biological activities of the produced essential oils were also investigated. Essential oils exhibited high insecticidal activities against Sitophilus granarius, as well as antioxidant, anti-inflammatory and moderate anti-plasmodial properties.


2020 ◽  
Vol 12 (1) ◽  
pp. 25-29
Author(s):  
Anju Bhatnagar

Cymbopogon species from the Poaceae family are widely distributed in the Himalayan region of India and commonly used as flavors, fragrances, cosmetics, and pharmaceuticals. It is known to contain compound citral, which give the lemon scent to many of the plants of the cymbopogon genera. The essential oil of  Cymbopogon flexuosus has high polyphenolic content which is responsible for antioxidant properties.  Beside citral is also used for the synthesis of vitamin B and Ionones. The bioactive potential of Lemongrass and constituent are rapidly increasing which is reflected from growing number of reports being published.  The present study was to know the chemical composition and in vitro antioxidant activity of essential oil of C. flexuosus from Uttarakhand. The essential oils of Cymbopogon collected in the region of Uttarakhand were obtained by hydrodistillation of the leaves and analyzed for chemical composition by GC/MS. The antioxidant activity of essential oils at different concentrations was determined against DPPH radical activity and vitamin C as the standard antioxidant compound.  The IC50    value and percentage of DPPH inhibition were recorded.  Twenty-five compounds were identified in essential oil extracted from leaves representing 93.15% of the oil composition. The yield of essential oil of Cymbopogon was 0.6 + 0.1 %and the major compound in the essential oil was citral (a racemic mixture of two isoforms geranial and nearl) followed by heptenone(1.98%) , linalool(1.65%), geraniol (1.47%), ?-caryophyllene (1.14% ) , limonene (0.92%),  nearl acetate (0.82%), citronellal(0.44 %) and citronellol (0.22%). Radical scavenging capacity (Inhibition, %) of the C. flexuosus essential oil was high (78.19+1.11) at the concentration level of 150 ?g/ml and  IC50 value of the essential oil was 43.67?g/ml.  The data of this study encourages to consider the essential oil of C. flexuosus  as a source of bioactive compounds which may add great industrial value to this crop.


Molecules ◽  
2019 ◽  
Vol 24 (2) ◽  
pp. 342 ◽  
Author(s):  
Wondwosen Matebie ◽  
Wanchang Zhang ◽  
Guangbo Xie

The essential oil from Phytolacca dodecandra, a traditional herb of Ethiopia, has been studied, including the chemical composition and antimicrobial activity. The difference between four P. dodecandra samples (P-1–P-4), which differed in gender or location, has also been analyzed. The essential oils were obtained by steam distillation, while the aromas were extracted by head space solid-phase microextraction (HS-SPME) and both were analyzed by gas chromatography- mass spectrometry (GC-MS). The oils’ antimicrobial activities were evaluated by the microdilution method against Escherichia coli, Staphylococcus aureus, Bacillus subtilis and Candida albicans. Ninety one components, representing 88.37 to 94.01% of the aromas, were identified. The compositions of the aromas of four samples are mainly dominated by aldehydes and ketones: 2-nonanone (1.80–30.80%), benzaldehyde (4.99–25.99%), and sulcatone (2.34–5.87%). Sixty components representing 64.61 to 69.64% of the oils were identified, and phytone (3.04–21.23%), phytol (4.11–26.29%) and palmitic acid (1.49–23.87%) are the major compounds. No obvious antimicrobial activity was observed for all the four essential oils.


2009 ◽  
Vol 4 (6) ◽  
pp. 1934578X0900400 ◽  
Author(s):  
Ana P. Murray ◽  
María S. Vela Gurovic ◽  
Silvana A. Rodriguez ◽  
María G. Murray ◽  
Adriana A. Ferrero

The essential oils of Schinus areira L. and S. longifolia (Lindl.) Speg. (Anacardiaceae) have been studied for their in vitro anti-acetylcholinesterase and antioxidant activities. The chemical composition of the oils obtained by hydrodistillation was determined by GC-MS. Fruit and leaf oils of S. areira were analyzed separately. The essential oil from S. longifolia elicited marked enzymatic inhibition (IC50 = 20.0 ± 1.0 μg/mL) and showed radical scavenger activity (IC50 = 25.2 ± 2.4 μg/mL). The essential oil from S. areira leaves was more active than that of the fruits in both bioassays.


2020 ◽  
Vol 15 (5) ◽  
pp. 1934578X2092781
Author(s):  
Farukh S Sharopov ◽  
Aminjon Salimov ◽  
Sodik Numonov ◽  
Abduahad Safomuddin ◽  
Mahinur Bakri ◽  
...  

The aerial parts of Аrtemisia annua L. were collected from Varzob, Rudaki, and Hisor regions of Tajikistan. The essential oil was obtained by hydrodistillation and analyzed by gas chromatography–mass spectrometry. The essential oils of aerial parts of А. annua were dominated by the monoterpenoids like camphor (32.5%-58.9%), 1,8-cineole (13.7%-17.8%), camphene (4.5%-8.4%), and α-pinene (1.9%-7.3%). Hierarchical cluster analysis of A. annua essential oils indicated the existence of 3 A. annua chemotypes: camphor/1,8-cineole, camphor, and artemisia ketone. The essential oils of А. annua show weak antioxidant activity and average antibacterial activity. In our opinion, the antibacterial activity of А. annua essential oils is related to the presence of 1,8-cineole. To our best knowledge, this is the first report concerning the chemical composition, chemotypic variation, antioxidant, and antimicrobial activities of the essential oils obtained from the aerial parts of А. annua, growing wild in Tajikistan.


Sign in / Sign up

Export Citation Format

Share Document