scholarly journals Micro-computed tomography: A novel diagnostic technique for the evaluation of gastrointestinal specimens

2021 ◽  
Vol 09 (12) ◽  
pp. E1886-E1889
Author(s):  
Noboru Kawata ◽  
Alexei Teplov ◽  
Peter Ntiamoah ◽  
Jinru Shia ◽  
Meera Hameed ◽  
...  
Keyword(s):  
Computed Tomography ◽  
Three Dimensional ◽  
Diagnostic Technique ◽  
Human Colon ◽  
Ffpe Tissue ◽  
Micro Ct ◽  
Micro Computed Tomography ◽  
Cross Sectional ◽  
Tissue Samples ◽  
Formalin Fixed Paraffin Embedded

AbstractMicro-computed tomography (micro-CT) is a non-destructive modality that can be used to obtain high-resolution three-dimensional (3 D) images of the whole sample tissue; the usefulness of micro-CT has been reported for evaluation of breast cancer and lung cancer. However, this novel diagnostic technique has never been used for evaluating endoscopically resected gastrointestinal specimens. In the present study, we scanned 13 formalin-fixed paraffin-embedded (FFPE) tissue blocks of a normal human colon and gastric tissue samples using micro-CT. The evaluation comprised a comparison of the acquired whole block images with the images of the corresponding cross-sectional slice of the hematoxylin and eosin-stained slide. Micro-CT was able to produce images of the whole sample and clearly depict tissues such as glandular structures, muscularis mucosae, and blood vessels in the FFPE tissue blocks of normal gastrointestinal samples. Furthermore, the 3 D reconstructed could be used to create a cross-sectional image and reflected the surface structure of samples obtained from any site. Micro-CT has the potential to become a highly promising pathological diagnostic assistance tool for endoscopically resected gastrointestinal specimens in combination with conventional microscopic examination.

scholarly journals Immunofluorescence-guided segmentation of three-dimensional features in micro-computed tomography datasets of human lung tissue

2021 ◽  
Vol 8 (11) ◽  
Author(s):  
Matthew J. Lawson ◽  
Orestis L. Katsamenis ◽  
David Chatelet ◽  
Aiman Alzetani ◽  
Oliver Larkin ◽  
...  
Keyword(s):  
Computed Tomography ◽  
Lung Tissue ◽  
Human Lung ◽  
Three Dimensional ◽  
Micro Computed Tomography ◽  
3D Segmentation ◽  
Human Lung Tissue ◽  
Formalin Fixed Paraffin ◽  
Formalin Fixed Paraffin Embedded ◽  
Formalin Fixed

Micro-computed tomography (µCT) provides non-destructive three-dimensional (3D) imaging of soft tissue microstructures. Specific features in µCT images can be identified using correlated two-dimensional (2D) histology images allowing manual segmentation. However, this is very time-consuming and requires specialist knowledge of the tissue and imaging modalities involved. Using a custom-designed µCT system optimized for imaging unstained formalin-fixed paraffin-embedded soft tissues, we imaged human lung tissue at isotropic voxel sizes less than 10 µm. Tissue sections were stained with haematoxylin and eosin or cytokeratin 18 in columnar airway epithelial cells using immunofluorescence (IF), as an exemplar of this workflow. Novel utilization of tissue autofluorescence allowed automatic alignment of 2D microscopy images to the 3D µCT data using scripted co-registration and automated image warping algorithms. Warped IF images, which were accurately aligned with the µCT datasets, allowed 3D segmentation of immunoreactive tissue microstructures in the human lung. Blood vessels were segmented semi-automatically using the co-registered µCT datasets. Correlating 2D IF and 3D µCT data enables accurate identification, localization and segmentation of features in fixed soft lung tissue. Our novel correlative imaging workflow provides faster and more automated 3D segmentation of µCT datasets. This is applicable to the huge range of formalin-fixed paraffin-embedded tissues held in biobanks and archives.

scholarly journals Micro-CT study of Middle Ordovician Spumellaria (radiolarians) from western Newfoundland, Canada

2020 ◽  
Vol 94 (3) ◽  
pp. 417-435 ◽  
Author(s):  
Sarah Kachovich ◽  
Jonathan C. Aitchison
Keyword(s):  
Computed Tomography ◽  
Three Dimensional ◽  
Detailed Examination ◽  
Micro Ct ◽  
Micro Computed Tomography ◽  
Lower Paleozoic ◽  
X Ray ◽  
Middle Ordovician ◽  
Radiolarian Assemblage ◽  
Taxonomic Groups

AbstractA new, previously undescribed Middle Ordovician (middle Darriwilian: Dw2) radiolarian assemblage has been recovered from the Table Cove Formation at Piccadilly Quarry, western Newfoundland. Constituents of the fauna described herein are both distinctive and exceptionally well preserved. Three-dimensional X-ray micro-computed tomography (μ-CT) is used to make a detailed examination of four key spumellarian specimens. This technology enables visualization of hitherto ambiguous details of the internal morphologies of key lower Paleozoic taxonomic groups, among which a lack of knowledge has impeded resolution of higher taxonomic rankings.

Upscaling Reservoir Rock Porosity by Fractal Dimension Using Three-Dimensional Micro-Computed Tomography and Two-Dimensional Scanning Electron Microscope Images

2020 ◽  
Vol 143 (1) ◽  
Author(s):  
Muhammad Jawad Munawar ◽  
Sandra Vega ◽  
Chengyan Lin ◽  
Mohammad Alsuwaidi ◽  
Naveed Ahsan ◽  
...  
Keyword(s):  
Computed Tomography ◽  
Three Dimensional ◽  
Fractal Dimensions ◽  
Two Dimensional ◽  
Micro Ct ◽  
Micro Computed Tomography ◽  
Sandstone Sample ◽  
The Core ◽  
Carbonate Sample ◽  
Scanning Electron

Abstract Scaling porosity of sedimentary rocks from the scale of measurement to the scale of interest is still a challenge. Upscaling of porosity can assist to accurately predict other petrophysical properties of rock at multiple scales. In this study, we use the two-dimensional (2D) scanning electron microscope (SEM) and three-dimensional (3D) X-ray micro-computed tomography (micro-CT) image to upscale porosity from the image scale to the core plug scale. A systematic imaging plan is deployed to capture rock properties of a carbonate and a sandstone sample, which are sensitive to the fractal nature of these rocks. Image analysis records wider pore spectrum (0.12–50 µm) in the carbonate sample than in sandstone (0.12–30 µm). The fractal dimensions are also higher in the carbonate than in the sandstone sample. Median, volume-weighted average of pore radius, and fractal dimensions derived from the image analysis are used as inputs in this equation. The results of the present study using this equation yielded to the best results on a resolution of 2.5 µm/voxel in the sandstone and 2.01 µm/voxel resolution in the carbonate sample for 3D micro-CT images, where fractal-scaling porosity matches well with the porosity measured at the core plug scale. The 2D SEM images provided a good estimation of porosity in the sandstone sample, where micro-CT imaging techniques could not capture the full pore spectrum. The fractal porosity equation showed promising results and offers a potential alternative way to estimate porosity when there are no routine core measurements available.

scholarly journals Evaluation of scaffold microstructure and comparison of cell seeding methods using micro-computed tomography-based tools

2020 ◽  
Vol 17 (165) ◽  
pp. 20200102
Author(s):  
Aleksi Palmroth ◽  
Sanna Pitkänen ◽  
Markus Hannula ◽  
Kaarlo Paakinaho ◽  
Jari Hyttinen ◽  
...  
Keyword(s):  
Computed Tomography ◽  
Three Dimensional ◽  
3D Cell Culture ◽  
Adipose Derived Stem Cells ◽  
Micro Ct ◽  
Cell Seeding ◽  
Micro Computed Tomography ◽  
Tissue Engineering Scaffolds ◽  
Scaffold Architecture ◽  
Static Seeding

Micro-computed tomography (micro-CT) provides a means to analyse and model three-dimensional (3D) tissue engineering scaffolds. This study proposes a set of micro-CT-based tools firstly for evaluating the microstructure of scaffolds and secondly for comparing different cell seeding methods. The pore size, porosity and pore interconnectivity of supercritical CO 2 processed poly( l -lactide-co- ɛ -caprolactone) (PLCL) and PLCL/β-tricalcium phosphate scaffolds were analysed using computational micro-CT models. The models were supplemented with an experimental method, where iron-labelled microspheres were seeded into the scaffolds and micro-CT imaged to assess their infiltration into the scaffolds. After examining the scaffold architecture, human adipose-derived stem cells (hASCs) were seeded into the scaffolds using five different cell seeding methods. Cell viability, number and 3D distribution were evaluated. The distribution of the cells was analysed using micro-CT by labelling the hASCs with ultrasmall paramagnetic iron oxide nanoparticles. Among the tested seeding methods, a forced fluid flow-based technique resulted in an enhanced cell infiltration throughout the scaffolds compared with static seeding. The current study provides an excellent set of tools for the development of scaffolds and for the design of 3D cell culture experiments.

scholarly journals Three Dimensional Imaging of Paraffin Embedded Human Lung Tissue Samples by Micro-Computed Tomography

PLoS ONE ◽  
2015 ◽  
Vol 10 (6) ◽  
pp. e0126230 ◽  
Author(s):  
Anna E. Scott ◽  
Dragos M. Vasilescu ◽  
Katherine A. D. Seal ◽  
Samuel D. Keyes ◽  
Mark N. Mavrogordato ◽  
...  
Keyword(s):  
Computed Tomography ◽  
Lung Tissue ◽  
Human Lung ◽  
Three Dimensional ◽  
Micro Computed Tomography ◽  
Human Lung Tissue ◽  
Three Dimensional Imaging ◽  
Tissue Samples

scholarly journals Imaging the aqueous humor outflow pathway in human eyes by three-dimensional micro-computed tomography (3D micro-CT)

2011 ◽  
Vol 92 (2) ◽  
pp. 104-111 ◽  
Author(s):  
Cheryl R. Hann ◽  
Michael D. Bentley ◽  
Andrew Vercnocke ◽  
Erik L. Ritman ◽  
Michael P. Fautsch
Keyword(s):  
Computed Tomography ◽  
Aqueous Humor ◽  
Three Dimensional ◽  
Micro Ct ◽  
Micro Computed Tomography ◽  
Aqueous Humor Outflow ◽  
Human Eyes ◽  
Outflow Pathway

Three-Dimensional Quantification of Calcium Salt-Composite Resorption (CSC) In Vitro by Micro-computed Tomography (Micro-CT)

JOM ◽  
2014 ◽  
Vol 66 (4) ◽  
pp. 559-565 ◽  
Author(s):  
T. Winkler ◽  
X. Y. Dai ◽  
G. Mielke ◽  
S. Vogt ◽  
H. Buechner ◽  
...  
Keyword(s):  
Computed Tomography ◽  
Calcium Salt ◽  
Three Dimensional ◽  
Micro Ct ◽  
Micro Computed Tomography

scholarly journals Cardiac Micro–Computed Tomography for Morphological and Functional Phenotyping of Muscle LIM Protein Null Mice

2007 ◽  
Vol 6 (4) ◽  
pp. 7290.2007.00022 ◽  
Author(s):  
Cristian T. Badea ◽  
Laurence W. Hedlund ◽  
Julie F. Boslego Mackel ◽  
Lan Mao ◽  
Howard A. Rockman ◽  
...  
Keyword(s):  
Computed Tomography ◽  
Three Dimensional ◽  
Blood Pool ◽  
Micro Ct ◽  
Micro Computed Tomography ◽  
Isotropic Resolution ◽  
Lim Protein ◽  
Muscle Lim Protein ◽  
The Difference ◽  
Time Required

The purpose of this study was to investigate the use of micro–computed tomography (micro-CT) for morphological and functional phenotyping of muscle LIM protein (MLP) null mice and to compare micro-CT with M-mode echocardiography. MLP null mice and controls were imaged using both micro-CT and M-mode echocardiography. For micro-CT, we used a custom-built scanner. Following a single intravenous injection of a blood pool contrast agent (Fenestra VC, ART Advanced Research Technologies, Saint-Laurent, QC) and using a cardiorespiratory gating, we acquired eight phases of the cardiac cycle (every 15 ms) and reconstructed three-dimensional data sets with 94-micron isotropic resolution. Wall thickness and volumetric measurements of the left ventricle were performed, and cardiac function was estimated. Micro-CT and M-mode echocardiography showed both morphological and functional aspects that separate MLP null mice from controls. End-diastolic and -systolic volumes were increased significantly three- and fivefold, respectively, in the MLP null mice versus controls. Ejection fraction was reduced by an average of 32% in MLP null mice. The data analysis shows that two imaging modalities provided different results partly owing to the difference in anesthesia regimens. Other sources of errors for micro-CT are also analyzed. Micro-CT can provide the four-dimensional data (three-dimensional isotropic volumes over time) required for morphological and functional phenotyping in mice.

scholarly journals Quantitative Analysis of the Anatomy of Mesial Roots of Mandibular First Molars with Vertucci Type I Root Canal Configuration by Means of Micro-Computed Tomography

2020 ◽  
Vol 10 (1) ◽  
pp. 17-21
Author(s):  
Ali Keleş ◽  
Cangül Keskin
Keyword(s):  
Computed Tomography ◽  
Quantitative Analysis ◽  
Root Canal ◽  
Visual Analysis ◽  
Descriptive Analysis ◽  
Type I ◽  
Micro Ct ◽  
Micro Computed Tomography ◽  
Cross Sectional ◽  
Root Canals

Aim:  This study aimed to evaluate root canal morphology of Vertucci type I root canal configuration detected in mesial roots of mandibular first molar teeth using micro-computed tomography (Micro-CT). Methodology: Micro-CT datasets of 269 specimens were evaluated for the detection of specimens with Vertucci type I root canal configuration for further analysis. Major and minor diameter, area, perimeter and roundness of root canal cross-sections at the beginning and midline of each root thirds were measured and recorded. The presence of apical delta, accessory canals, and deviation of major apical foramen from anatomical apex were investigated. Data was analyzed using descriptive analysis, ANOVA-Tukey and Kruskal-Wallis H-Wilcoxon signed-rank tests. Results: Vertucci type I canal configuration was present in the 4.46% of the specimens. The specimens often showed accessory root canals (50%) and apical deltas (41,6%). From the visual analysis of the 3D and 2D images, significant differences were detected among coronal, middle and apical thirds of the specimens regarding major and minor diameter, area, perimeter and roundness (P < 0.05). Conclusion: Three- and two-dimensional analyses of mesial roots with Vertucci type I canal configuration indicated that this type of configuration presents large buccolingual diameters with long oval shape and has isthmus at coronal and middle thirds. At the apical third the root canals often terminate in an oval cross sectional shape.   How to cite this article: Keles A, Keskin C. Quantitative analysis of the anatomy of mesial roots of mandibular first molars with Vertucci type I root canal configuration by means of micro-computed tomography. Int Dent Res 2020;10(1):17-21. https://doi.org/10.5577/intdentres.2020.vol10.no1.4   Linguistic Revision: The English in this manuscript has been checked by at least two professional editors, both native speakers of English.

Sign in / Sign up

Export Citation Format

Share Document