Comparative study of the effect of a herbal preparation (Imupret®) dissolved in different solvents on LPS-primed type II-like human lung epithelial A549 cells

ABSTRACT Human parainfluenza virus type 3 (HPIV-3) is an airborne pathogen that infects the epithelial cells of the respiratory tract. In the present study we investigated the interaction of HPIV-3 with the type II alveolar human lung polarized epithelial A549 cells. Although HPIV-3 entry and budding were bidirectional from both the apical and the basolateral domains, HPIV-3 exhibited preferential entry and release from the apical pole. While disruption of the cellular actin microfilament and microtubule by cytochalasin D and nocodazole, respectively, had no effect on virus entry, disruption of the microtubule but not the microfilament inhibited HPIV-3 release.

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A herbal preparation Imupret® inhibits LPS and IL-1β-induced release of IL-8 and human β-defensin 2 in lung epithelial A549 cells

Planta Medica ◽

10.1055/s-0028-1083989 ◽

2008 ◽

Vol 74 (09) ◽

Author(s):

K Hostanska ◽

J Melzer ◽

A Amon ◽

R Saller

Keyword(s):

A549 Cells ◽

Herbal Preparation ◽

Lung Epithelial

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Comparative Toxic Effects of Manufactured Nanoparticles and Atmospheric Particulate Matter in Human Lung Epithelial Cells

International Journal of Environmental Research and Public Health ◽

10.3390/ijerph18010022 ◽

2020 ◽

Vol 18 (1) ◽

pp. 22

Author(s):

Yun Wu ◽

Mei Wang ◽

Shaojuan Luo ◽

Yunfeng Gu ◽

Dongyang Nie ◽

...

Keyword(s):

Particulate Matter ◽

Human Lung ◽

A549 Cells ◽

Lung Epithelial Cells ◽

Atmospheric Particulate Matter ◽

Toxic Effects ◽

Ros Generation ◽

Atmospheric Particulate ◽

Significant Difference ◽

Lung Epithelial

Although nanoparticles (NPs) have been used as simplified atmospheric particulate matter (PM) models, little experimental evidence is available to support such simulations. In this study, we comparatively assessed the toxic effects of PM and typical NPs (four carbonaceous NPs with different morphologies, metal NPs of Fe, Al, and Ti, as well as SiO2 NPs) on human lung epithelial A549 cells. The EC50 value of PM evaluated by cell viability assay was 148.7 μg/mL, closest to that of SiO2 NPs, between the values of carbonaceous NPs and metal NPs. All particles caused varying degrees of reactive oxygen species (ROS) generation and adenosine triphosphate (ATP) suppression. TiO2 NPs showed similar performance with PM in inducing ROS production (p < 0.05). Small variations between two carbonaceous NPs (graphene oxides and graphenes) and PM were also observed at 50 μg/mL. Similarly, there was no significant difference in ATP inhibition between carbonaceous NPs and PM, while markedly different effects were caused by SiO2 NP and TiO2 NP exposure. Our results indicated that carbonaceous NPs could be served as potential surrogates for urban PM. The identification of PM model may help us further explore the specific roles and mechanisms of various components in PM.

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Enhancing Effect of Poly(amino acid)s on Albumin Uptake in Human Lung Epithelial A549 Cells

Drug Metabolism and Pharmacokinetics ◽

10.2133/dmpk.dmpk-13-rg-028 ◽

2013 ◽

Vol 28 (6) ◽

pp. 497-503 ◽

Cited By ~ 4

Author(s):

Ryoko Yumoto ◽

Sayuri Suzuka ◽

Saori Nishimoto ◽

Junya Nagai ◽

Mikihisa Takano

Keyword(s):

Amino Acid ◽

Human Lung ◽

A549 Cells ◽

Enhancing Effect ◽

Lung Epithelial

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Bioactive extracts of Ziziphus mauritiana induces apoptosis in A549 human lung epithelial carcinoma cells through the generation of reactive oxygen species

Current Cancer Therapy Reviews ◽

10.2174/1573394717666210805115802 ◽

2021 ◽

Vol 17 ◽

Author(s):

Om Prakash ◽

Shazia Usmani ◽

Amresh Gupta ◽

Asif Jafri ◽

Mohammad Fahad Ullah ◽

...

Keyword(s):

Mtt Assay ◽

Human Lung ◽

A549 Cells ◽

Carcinoma Cells ◽

Ziziphus Mauritiana ◽

Meoh Extract ◽

Lung Carcinomas ◽

Pharmacological Significance ◽

Lung Epithelial ◽

A549 Lung

Background: In recent years, novel metabolites isolated from botanical sources have attracted much attention for traditional and therapeutic significance. The ethnopharmacological studies suggest that Ziziphus mauritiana is a common remedy against several kinds of ailments. Objective: The current study has evaluated the MeOH extract of Ziziphus mauritiana leaves (ZME) through physicochemical, phytochemical, and chromatographic fingerprinting analysis, which displayed an array of biometabolites of pharmacological significance including flavonoids. Methods: The extract was further examined for anticancer activities which revealed promising anticancer properties against human lung epithelial carcinoma cells (A549) and induction of apoptosis impart by ROS. The oxidative stress was evaluated in terms of production and accumulation of cytosolic extent of ROS whereas anticancer perspective was determined by MTT assay, cell morphology analysis, followed by nuclear condensation for the examination of apoptosis induction. Results: Finding suggests that the MeOH extract of ZME markedly exhibited promising anticancer activity against the A549 lung epithelial carcinoma cell. The ZME was found to be most active in the MTT assay against A549 cells while it was less toxic to normal cells. The intracellular ROS generation was remarkably induced by ZME, which correlated with the ability of the flavonoid-rich fractions in the MeOH extract to inhibit cell growth and might induce apoptosis. Conclusion: The present study provides useful insight concerning the promising anticancer potential of ZME against A549 lung carcinomas. However, the clinical correlation will be required for its authorization and in the discovery of significant and least noxious novel agents against lung carcinomas.

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Ionizing radiation enhances matrix metalloproteinase-2 production in human lung epithelial cells

AJP Lung Cellular and Molecular Physiology ◽

10.1152/ajplung.2001.280.1.l30 ◽

2001 ◽

Vol 280 (1) ◽

pp. L30-L38 ◽

Cited By ~ 41

Author(s):

Jun Araya ◽

Muneharu Maruyama ◽

Kazuhiko Sassa ◽

Tadashi Fujita ◽

Ryuji Hayashi ◽

...

Keyword(s):

Epithelial Cells ◽

Ionizing Radiation ◽

Basement Membrane ◽

Lung Injury ◽

Human Lung ◽

A549 Cells ◽

Lung Epithelial Cells ◽

Lung Epithelial ◽

Radiation Induced ◽

Human Lung Epithelial Cells

Radiation pneumonitis is a major complication of radiation therapy. However, the detailed cellular mechanisms have not been clearly defined. Based on the recognition that basement membrane disruption occurs in acute lung injury and that matrix metalloproteinase (MMP)-2 can degrade type IV collagen, one of the major components of the basement membrane, we hypothesized that ionizing radiation would modulate MMP-2 production in human lung epithelial cells. To evaluate this, the modulation of MMP-2 with irradiation was investigated in normal human bronchial epithelial cells as well as in A549 cells. We measured the activity of MMP-2 in the conditioned medium with zymography and the MMP-2 mRNA level with RT-PCR. Both of these cells constitutively expressed 72-kDa gelatinolytic activity, corresponding to MMP-2, and exposure to radiation increased this activity. Consistent with the data of zymography, ionizing radiation increased the level of MMP-2 mRNA. This radiation-induced increase in MMP-2 expression was mediated via p53 because the p53 antisense oligonucleotide abolished the increase in MMP-2 activity as well as the accumulation of p53 after irradiation in A549 cells. These results indicate that MMP-2 expression by human lung epithelial cells is involved in radiation-induced lung injury.

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Identification of novel antiviral drug combinations in vitro and tracking their development

10.1101/2020.09.17.299933 ◽

2020 ◽

Author(s):

Aleksandr Ianevski ◽

Rouan Yao ◽

Svetlana Biza ◽

Eva Zusinaite ◽

Andres Männik ◽

...

Keyword(s):

Viral Infections ◽

Human Lung ◽

Antiviral Drug ◽

A549 Cells ◽

Drug Combinations ◽

Investigational Drug ◽

Synergistic Activity ◽

Lung Epithelial ◽

Reduced Toxicity

AbstractCombination therapies have become a standard for the treatment for HIV and HCV infections. They are advantageous over monotherapies due to better efficacy and reduced toxicity, as well as the ability to prevent the development of resistant viral strains and to treat viral co-infections. Here, we identify several new synergistic combinations against emerging and re-emerging viral infections in vitro. We observed synergistic activity of nelfinavir with investigational drug EIDD-2801 and convalescent serum against SARS-CoV-2 infection in human lung epithelial Calu-3 cells. We also demonstrated synergistic activity of vemurafenib combination with emetine, homoharringtonine, gemcitabine, or obatoclax against echovirus 1 infection in human lung epithelial A549 cells. We also found that combinations of sofosbuvir with brequinar and niclosamide were synergistic against HCV infection in hepatocyte derived Huh-7.5 cells, whereas combinations of monensin with lamivudine and tenofovir were synergistic against HIV-1 infection in human cervical TZM-bl cells. Finally, we present an online resource that summarizes novel and known antiviral drug combinations and their developmental status. Overall, the development of combinational therapies could have a global impact improving the preparedness and protection of the general population from emerging and re-emerging viral threats.

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