scholarly journals Effect of stalk and leaf extracts from Euphorbiaceae species on Aedes aegypti (Diptera, Culicidae) larvae

2006 ◽  
Vol 48 (4) ◽  
pp. 211-214 ◽  
Author(s):  
Maria Goretti Araújo de Lima ◽  
Ismália Cassandra Costa Maia ◽  
Bruna Dantas de Sousa ◽  
Selene Maia de Morais ◽  
Sílvia Maria Freitas

The objective of this study was to evaluate the larvicidal activity of essential oil aqueous solutions (hydrolates) obtained by steam distillation of stalks and leaves of Croton argyrophylloides, Croton nepetaefolius, Croton sonderianus and Croton zehntneri against Aedes aegypti larvae. Twenty-five larvae of third instar were placed in plastic beckers, containing the hydrolates (50 mL), in a four repetitions scheme. Water was used as control and the number of dead larvae was counted after 24 hours. The data obtained were submitted to Variance Analysis and Tukey test. Significant differences were observed among the hydrolates from different species and from different parts of each plant (p < 0.001). The hydrolates of stalk and leaf from C. nepetaefolius and C. zehntneri and leaf hydrolate of C. argyrophylloides presented 100% mortality against larvae. The compounds present in C. zenhtneri and C. nepetaefolius are oxygenated phenylpropanoids that are more soluble in water than the monoterpenes and sesquiterpenes detected in the oils of C. argyrophylloides and C. sonderianus. This study showed that all species analyzed presented compounds with larvicidal properties, with differences between each plant parts.

2010 ◽  
Vol 5 (5) ◽  
pp. 1934578X1000500 ◽  
Author(s):  
Jesús Palá-Paúl ◽  
Jaime Usano-Alemany ◽  
Joseph J. Brophy ◽  
María J. Pérez-Alonso ◽  
Ana-Cristina Soria

The essential oils from the different parts [inflorescences (E.a.I), stems + leaves (E.a.SL) and roots (E.a.R)] of E. aquifolium Cav. gathered in Cádiz (Spain), have been extracted by steam distillation and analyzed by gas chromatography and gas chromatography coupled to mass spectrometry. Quantitative and qualitative differences have been found between the analyzed plant parts. A total of 107 compounds have been identified. The main constituents were germacrene D (30.3%) and sesquicineole (26.7%) for E.a.I fraction, germacrene D (46.0%) and myrcene (13.8%) in the E.a.SL, while E.a.R showed phyllocladene isomer (63.6%) as a unique major compound. The percentage composition of the other constituents was lower than 5.5% in all the analyzed fractions. In agreement with other Eryngium species, no specific compound could be used as a marker for the chemotaxonomy of E. aquifolium. However, similarities in volatile composition were found between E. aquifolium and other species growing under similar environmental conditions. As far as we know, this is the first report on the essential oil of this species.


Author(s):  
Anne Carolina ◽  
Maman Maman

The aim of this research was to examine the larvicidal activity of essential oil (EO) extracted from nutmeg (Myristica fragrans Houtt) leaves and fruits by steam distillation, and to analyze its chemical compounds. The EO yield of nutmeg leaves and fruits collected from the same tree was 0.66% and 0.30%, respectively. Larvicidal tests with the EO were carried out against Aedes aegypti (L.) (Diptera: Culicidae). The concentrations of nutmeg EO used for the larvicidal assay were 50, 100, 150, 200, and 250 μg/mL. The results showed that fruit oil was more toxic than the leaf oil. LC50 values of leaf and fruit EOs were 133.8 and 110.1 µg/mL, respectively. The chromatogram of GC-MS showed that the chemical components in nutmeg leaf and fruit EOs were dominated by α-pinene, sabinene, β-pinene, delta-3-carene, limonene, β-phellandrene, α-terpinolene, linalool, safrole, croweacin, and myristicin.


2017 ◽  
Vol 54 (3) ◽  
pp. 670-676 ◽  
Author(s):  
Agustín Alvarez Costa ◽  
Cecilia V. Naspi ◽  
Alejandro Lucia ◽  
Héctor M. Masuh

2007 ◽  
Vol 2 (12) ◽  
pp. 1934578X0700201 ◽  
Author(s):  
Hélcio S. Santos ◽  
Gilvandete M. P. Santiago ◽  
João P. P. de Oliveira ◽  
Angela M. C. Arriaga ◽  
Délcio D. Marques ◽  
...  

The chemical composition of the essential oils from leaves, stalks and inflorescences of Croton zehntneri obtained by hydrodistillation were analyzed by GC-MS and CG-FID. E-Anethole was the main component of the essential oils of all plant parts. Essential oils of leaves, stalks, inflorescences and E-anethole were tested at different concentrations against instar III larvae of Aedes aegypti and showed LC50 values of 56.2 ± 0.3, 51.3 ± 0.3, 57.5 ± 0.1 and 69.2 ± 0.5 μg/mL, respectively.


2018 ◽  
Vol 30 (5) ◽  
pp. 379-387 ◽  
Author(s):  
Carla Maria Mariano Fernandez ◽  
Maurício Ferreira da Rosa ◽  
Ana Claudia Aparecida Mariano Fernandez ◽  
Fabiana Brusco Lorenzetti ◽  
Keila Fernanda Raimundo ◽  
...  

Molecules ◽  
2020 ◽  
Vol 25 (6) ◽  
pp. 1359
Author(s):  
Abbas Ali ◽  
Nurhayat Tabanca ◽  
Betul Demirci ◽  
Vijayasankar Raman ◽  
Jane M. Budel ◽  
...  

In our natural products screening program for mosquitoes, we tested essential oils extracted from different plant parts of Magnolia grandiflora L. for their insecticidal and biting deterrent activities against Aedes aegypti. Biting deterrence of seeds essential oil with biting deterrence index value of 0.89 was similar to N,N-diethyl-3-methylbenzamide (DEET). All the other oils were active above the solvent control but the activity was significantly lower than DEET. Based on GC-MS analysis, three pure compounds that were only present in the essential oil of seed were further investigated to identify the compounds responsible for biting deterrent activity. 1-Decanol with PNB value of 0.8 was similar to DEET (PNB = 0.8), whereas 1-octanol with PNB value of 0.64 showed biting deterrence lower than 1-decanol and DEET. The activity of 1-heptanol with PNB value of 0.36 was similar to the negative control. Since 1-decanol, which was 3.3% of the seed essential oil, showed biting deterrence similar to DEET as a pure compound, this compound might be responsible for the activity of this oil. In in vitro A & K bioassay, 1-decanol with MED value of 6.25 showed higher repellency than DEET (MED = 12.5). Essential oils of immature and mature fruit showed high toxicity whereas leaf, flower, and seeds essential oils gave only 20%, 0%, and 50% mortality, respectively, at the highest dose of 125 ppm. 1-Decanol with LC50 of 4.8 ppm was the most toxic compound.


2020 ◽  
Vol 17 (34) ◽  
pp. 1-9
Author(s):  
Andréia Gregório da Silva SANTOS ◽  
Daniela do Amaral Ferraz NAVARRO ◽  
Nereide Stela Santos MAGALHÃES ◽  
Maria Alice Varjal MELO-SANTOS ◽  
Sofia Suely Ferreira BRANDÃO

Aedes aegypti is a species of mosquito with wide distribution worldwide, involved in the cycles of transmission of dengue, Zika and chikungunya. The lack of vaccines for most of these arboviruses highlights the importance of vector control as a measure to reduce the incidence of these diseases. Several researches have been carried out in order to find substances of plant origin that are candidates for the control of A. aegypti. In this sense, medicinal plants that also have an insecticidal action have shown promise for the development of new products, environmentally safer than traditional chemical insecticides. The objective of this work was to evaluate the larvicidal activity of the essential oil of the leaves of Croton rhamnifolioides and its formulation in nanocapsules for A. aegypti. The essential oil was extracted by hydrodistillation and analyzed by gas chromatography coupled to a mass spectrometer (CG/MS). Formulations were produced containing concentrations of pure oil, which ranged from 40 to 80 μg/mL and polycaprolactone nanocapsules with concentrations from 30 to 120 μg/mL. Larvicide tests were performed using varying concentrations of an aqueous suspension, in triplicates, containing 20 larvae / concentration, in addition to an untreated control. As a result, the essential oil had an average content that varied between 1.4% and 0.58%, depending on the time of collection and, as main constituents, eucalyptol (16.57%) and (E) -cariophylene (11, 32%). LC50 values ranged from 26.3 μg/mL to 52.2 μg/mL and CL90 from 37.8 μg/mL to 68.7 μg/mL for pure oil. In encapsulated oil, the LC50 = 63.4 μg/mL and the LC90 = 104.8 μg/mL. The pure oil of Croton rhamnifolioides was considered more active against the larvae of A. aegypti than the encapsulated one, although both have great potential for application as a larvicide.


2019 ◽  
Vol 11 (5) ◽  
pp. 314
Author(s):  
Bruna de Villa ◽  
Reginaldo Ferreira Santos ◽  
Deonir Secco ◽  
Jair Antonio Cruz Siqueira ◽  
Luciene Kazue Tokura ◽  
...  

The crambe crop is an interesting option for the production of biodiesel, mainly due to the high oil content (35%) and the corn one of the most implanted crops in Brazil. Thus, the objective of this work was to evaluate the allelopathic effect of crambe in the initial development of maize. The experiment was carried out in the laboratory of seed and plant evaluation (LASP) and laboratory of soil physics (LAFIS) of UNIOESTE-Cascavel. The seeds of corn with germination of 93.5% were submitted to four concentrations plus the control in the different parts of the crambe plant, where the growth and mass of corn were valued. The experimental design was completely randomized with 4 replicates per treatment. The statistical analysis consisted of analysis of variance and the means of the treatments were compared by the Tukey test at 5% of significance. The extract of the whole crambe plant stimulated aerial length, root length, fresh and dry mass of corn shoot. Root, stem and leaf extracts inhibited the initial development of maize in relation to growth and fresh and dry shoot mass.


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