Weed-Associated Fungal Endophytes as Biocontrol Agents of Fusariumoxysporum f. sp. cubense TR4 in Cavendish Banana

The antagonistic activity of fungal endophytes isolated from weeds growing in Cavendish banana farms was determined against Fusarium oxysporum f. sp. cubense TR4 (Foc TR4) causing Fusarium wilt of Cavendish banana. Forty-nine out of the total 357 fungal endophytes from the roots of weeds exhibited antagonistic activity against Foc TR4. High inhibitory activity at 79.61–99.31% based on dual culture assay was recorded in endophytes Lasiodiplodia theobromae TDC029, Trichoderma asperellum TDC075, Ceratobasidium sp. TDC037, Ceratobasidium sp. TDC241, and Ceratobasidium sp. TDC474. All five endophytes were identified through DNA sequencing with 86–100% identity. Endophyte-treated Grand Naine and GCTCV 218 plantlets showed significantly lower disease incidence (p = 0.014), significantly lower degree of leaf yellowing (p = 0.037) and rhizome discoloration (p = 0.003). In addition, the cultivar Grand Naine was consistently highly susceptible compared with the tolerant cultivar GCTCV 218.

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Exploration of multitrait antagonistic microbes against Fusarium oxysporum f.sp. lycopersici

Journal of Applied and Natural Science ◽

10.31018/jans.v11i2.2111 ◽

2019 ◽

Vol 11 (2) ◽

pp. 503-510 ◽

Cited By ~ 2

Author(s):

Prachi Singh ◽

Jyoti Singh ◽

Rahul Singh Rajput ◽

Anukool Vaishnav ◽

Shatrupa Ray ◽

...

Keyword(s):

Fusarium Oxysporum ◽

Fusarium Wilt ◽

Cost Effective ◽

Field Studies ◽

Dual Culture ◽

Cost Effective Approach ◽

Maximum Inhibition ◽

Plant Growth Promoting Activities ◽

Plant Growth Promoting ◽

Dual Culture Assay

Fusarium wilt is one of the major diseases of tomato causing extensive loss of production. Exploration of agriculturally important microbes (AIMs) for management of the tomato wilt is an ecofriendly and cost effective approach. In the present study, a total 30 Trichoderma and 30 bacterial isolates were screened in the laboratory for their biocontrol activity against Fusarium oxysporum f.sp. lycopersici (FOL). Out of all the isolates tested, Trichoderma asperellum BHU P-1 and Ochrobactrum sp. BHU PB-1 were found to show maximum inhibition of FOL in dual culture assay. Both the microbes also exhibited plant growth promoting activities such as phosphate solubilisation, production of siderophore, hydrogen cyanide (HCN), indole acetic acid (IAA) and protease activity. These microbes could be evaluated further in greenhouse and field studies for their potential use in management of Fusarium wilt of tomato.

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Inhibitory Effect of Trichoderma asperellum Isolate against Ralstonia solanacearum Causing Brinjal Wilt

Annals of Bangladesh Agriculture ◽

10.3329/aba.v24i2.55788 ◽

2021 ◽

Vol 24 (2) ◽

pp. 107-120

Author(s):

SMN Islam ◽

SS Siddique ◽

MZH Chowdhury ◽

NJ Mishu

Keyword(s):

Ralstonia Solanacearum ◽

Disease Incidence ◽

Its Region ◽

Inhibitory Effect ◽

Dual Culture ◽

Trichoderma Asperellum ◽

In Planta ◽

Quantitative Test ◽

Trichoderma Isolate

A native Trichoderma isolate was collected from the agricultural soil of Gazipur. This isolate was identified as a Trichoderma asperellum through morphology and analysis of internal transcribed spacer (ITS) region of ribosomal RNA gene sequence and reconstruction of the phylogenetic tree. The antagonistic effects of the newly identified T. asperellum isolate were assessed against brinjal bacterial wilt caused by Ralstonia solanacearum both in vitro and in planta. Both qualitative and quantitative bioassays were conducted in vitro. For qualitative tests, dual culture and antibacterial activity were carried out, and pathogen growth was observed visually. The antagonism of T. asperellum cell free culture filtrate on the growth of R. solanacearum was conducted in a quantitative test. Successful antagonism was recorded after both in vitro qualitative tests. In addition, the lowest colony forming unit was recorded in 100% of CFC (2.4±0.51 ×103 cfu/ml) in quantitative test. The T. asperellum inoculated plant showed low disease incidence (13.33%) when seedlings were challenged with R. solanacearum in planta experiment. Disease incidence was 100% for seedlings when treated with only R. solanacearum. The results showed that the isolated and identified T. asperellum isolate suppressed R. solanacearum growth in vitro and protected the seedling from wilting in planta. Therefore, this isolate could be considered as a potential isolate. Ann. Bangladesh Agric. (2020) 24(2) : 107-120

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Role of Biocontrol Agents in Management of Corm Rot of Saffron Caused by Fusarium oxysporum

Agronomy ◽

10.3390/agronomy10091398 ◽

2020 ◽

Vol 10 (9) ◽

pp. 1398

Author(s):

Vishal Gupta ◽

Krishna Kumar ◽

Kausar Fatima ◽

Vijay Kumar Razdan ◽

Bhagwati Charan Sharma ◽

...

Keyword(s):

Fusarium Oxysporum ◽

Growth Promotion ◽

Disease Incidence ◽

Biocontrol Agents ◽

Crocus Sativus ◽

First Year ◽

Dual Culture ◽

Maximum Reduction

Saffron (Crocus sativus L.) is considered as one of the most expensive spices. Fusarium corm rot of saffron, caused by Fusarium oxysporum, is known to cause severe yield losses worldwide. In the present study, efficacy of biocontrol agents (Trichoderma asperellum, Pseudomonas fluorescens, Pseudomonas aeruginosa, Pseudomonas putida, Bacillus stratosphericus, Bacillus pumilus, and Bacillus subtilis) along with a chemical fungicide, carbendazim, was evaluated for managing the corm rot of saffron. Under in vitro conditions, using dual culture and poison food techniques on potato dextrose agar, T. asperellum and carbendazim significantly reduced the mycelial growth of the pathogen F. oxysporum, with the inhibition of 62.76 and 60.27%, respectively, compared with control. Under field conditions, dipping of saffron corms in carbendazim and T. asperellum exhibited maximum reduction of 82.77 and 77.84%, respectively, in the disease incidence, during the first year of experiment. However, during the second year, maximum reduction in the incidence of corm rot (68.63%) was recorded with the T. asperellum. Moreover, the population density of F. oxysporum was also significantly reduced by 60 and 80.19% while using T. asperellum after 75 and 260 days of sowing of saffron corms, compared to its population before planting of corms. In case of growth promotion traits, such as sprouting and flowering, biocontrol treatments reduced the number of days (average) of sprouting and flower emergence after sowing, compared to control.

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Antagonistic Activity and Characterization of Indigenous Soil Isolates of Bacteria and Fungi Against Onion Wilt Incited by Fusarium sp

10.21203/rs.3.rs-250917/v1 ◽

2021 ◽

Author(s):

Hilda Karim ◽

Andi Asmawati ◽

Oslan Jumadi

Keyword(s):

Fusarium Oxysporum ◽

Evolutionary Relationship ◽

Antagonistic Activity ◽

Dual Culture ◽

Bacterial Isolates ◽

Gene Markers ◽

Culture Techniques ◽

Fungal Isolates ◽

Bacteria And Fungi

Abstract Tuber rot disease due to phytopathogen Fusarium oxysporum f. sp. cepae (Foc) infection is one of the main factors causing the decreasing amount of global shallot production. This study aims to find bacteria and fungi candidates which have Foc antagonistic activity through in vitro tests using dual culture techniques. A total of five bacterial isolates and three fungal isolates isolated from the rhizosphere of healthy onion plants showed the ability to inhibit Foc growth. B1 and B4 bacterial isolates had an average inhibitory capability of 65.93% and 72.27% respectively. Whereas C1 and C2 fungal isolates have the ability to inhibit the growth of Foc by as much as 74.82% and 67.76% respectively. The four tested microbial isolates were able to significantly inhibit Foc activity in vitro based on the ANOVA test, with values α = 0.05, and n = 3. Molecular analysis based on 16S-rRNA markers showed bacterial isolates B1 and B4 have an evolutionary relationship with B. subtilis. Whereas fungi C1 and C2 have evolutionary relationships with Aspergillus tubingensis and Trichoderma asperellum respectively, based on internal transcribed spacer (ITS) gene markers. The results of this study can be used to develop indigenous microbial consortiums as biological control agents for phytopathogenic fungi Fusarium oxysporum f. sp. cepae (Foc) on shallots.

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Use of Trichoderma in Biological Control of Collar Rot of Soybean and Chickpea

International Journal of Biochemistry Research & Review ◽

10.9734/ijbcrr/2020/v29i930221 ◽

2020 ◽

pp. 25-31

Author(s):

Mahbuba Kaniz Hasna ◽

Md. Abul Kashem ◽

Farid Ahmed

Keyword(s):

Seed Treatment ◽

Field Experiments ◽

Disease Incidence ◽

Sclerotium Rolfsii ◽

Antagonistic Activity ◽

Root Weight ◽

Dual Culture ◽

Collar Rot ◽

Rot Disease

An in vitro and field experiments for two consecutive years were conducted at Bangladesh Institute of Nuclear Agriculture, Mymensingh, aiming to investigate the efficacy of Trichoderma harzianum against Sclerotium rolfsii causing collar rot disease of soybean and chickpea. In in vitro the antagonistic activity of T. harzianum against S. rolfsii was observed through dual culture. In field experiment Trichoderma was applied as soil treatment and seed treatment. The percent inhibition of S. rolfsii induced by T. harzianum was found upto 78.9% in in vitro. The maximum reduction of collar rot disease incidence over control was 82.4% in soybean and 77.6% in chickpea which was recorded in the plot where T. harzianum was applied in the soil. The highest seed germination: 86.3% in soybean and 84.8% in chickpea, maximum fresh shoot weight: 94.5 g plant-1 in soybean, 62.5 g plant-1 in chickpea, maximum fresh root weight: 10.7 g plant-1 in soybean, 9.3 g plant-1 in chickpea and the highest yield: 2830 kg ha-1 in soybean, 1836 kg ha-1 in chickpea were obtained by the application of Trichoderma in soil. The study indicated that the tested isolate of T. harzianum had potential in controlling collar rot disease of soybean and chickpea. For the reduction of collar rot incidence application of T. harzianum in soil was found more effective than seed treatment.

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Isolation, Identification and In Vitro Test for the Biocontrol Potential of Trichoderma viride on Fusarium oxysporum f. sp. Lycopersici

The Open Agriculture Journal ◽

10.2174/1874331502115010010 ◽

2021 ◽

Vol 15 (1) ◽

pp. 10-20

Author(s):

Tsegaye Mekuria Ayele ◽

Guesh Desta Gebremariam ◽

Subban Patharajan

Keyword(s):

Fusarium Oxysporum ◽

Trichoderma Viride ◽

Dual Culture ◽

In Vitro Test ◽

Tomato Production ◽

Biocontrol Efficacy ◽

Pests And Diseases ◽

Dual Culture Assay ◽

Biocontrol Potential

Introduction: Tomato production in Ethiopia is challenged by many pests and diseases. Fusarium wilt is one of the most important diseases of tomato affecting its productivity. Methods: Tomato tissue and soil samples were collected from tomato farmlands around Aksum town to isolate and identify pathogenic Fusarium species and Trichoderma species with biocontrol efficacy. Samples were processed in the Aksum University Biotechnology laboratory following standard procedures. Results and Discussion: Eight Fusarium and five Trichoderma isolates were obtained. Six of the Fusarium isolates were identified as Fusarium oxysporum, whereas the remaining two were Fusarium equiseti and Fusarium circinatum. Detached leaf bioassay of the F. oxysporum on tomato leaves showed leaf lesion on the tomato variety, Melka oda. The isolated Trichoderma strains were screened for biocontrol potential against virulent F. oxysporum in vitro. The Trichoderma isolate showing the highest biocontrol efficacy against the virulent Fusarium was morphologically identified as Trichoderma viride. in vitro F. oxysporum-T. viride dual culture assay demonstrated that T. viride inhibits the growth of F. oxysporum f.sp. lycopersici with 76.94% growth inhibition. Conclusion: Fusarium oxysporum is prevalent in tomato growing farmlands covered in this study. T. viride identified in this study is an effective biocontrol agent for the identified F. oxysporum fsp. lycopersici in vitro.

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Cladobotryum mycophilum as Potential Biocontrol Agent

Agronomy ◽

10.3390/agronomy9120891 ◽

2019 ◽

Vol 9 (12) ◽

pp. 891

Author(s):

Mila Santos ◽

Fernando Diánez ◽

Alejandro Moreno-Gavíra ◽

Brenda Sánchez-Montesinos ◽

Francisco J. Gea

Keyword(s):

Biological Control ◽

Fusarium Oxysporum ◽

Biocontrol Agent ◽

Biological Control Agent ◽

Control Agent ◽

Phytopathogenic Fungi ◽

Antagonistic Activity ◽

Dual Culture ◽

Potential Biocontrol Agent

A study was conducted to explore the efficacy of potential biocontrol agent Cladobotryum mycophilum against different phytopathogenic fungi. The growth rates of 24 isolates of C. mycophilum were determined, and their antagonistic activity was analysed in vitro and in vivo against Botrytis cinerea, Fusarium oxysporum f. sp. radicis-lycopersici, Fusarium oxysporum f.sp. cucumerinum, Fusarium solani, Phytophthora parasitica, Phytophthora capsici, Pythium aphanidermatum and Mycosphaerella melonis. Most isolates grow rapidly, reaching the opposite end of the Petri dish within 72–96 h. Under dual-culture assays, C. mycophilum showed antagonistic activity in vitro against all phytopathogenic fungi tested, with mycelial growth inhibition ranging from 30 to 90% against all the different phytopathogens tested. Similarly, of all the selected isolates, CL60A, CL17A and CL18A significantly (p < 0.05) reduced the disease incidence and severity in the plant assays compared to the controls for the different pathosystems studied. Based on these results, we conclude that C. mycophilum can be considered as a potential biological control agent in agriculture. This is the first study of Cladobotryum mycophilum as a biological control agent for different diseases caused by highly relevant phytopathogens in horticulture.

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ANTAGONISTIC TEST OF RIAU LOCAL FUNGAL ISOLATES AGAINST SOME PATHOGENIC IN CULTIVATED PLANTS

AL-Kauniyah Jurnal Biologi ◽

10.15408/kauniyah.v12i2.8730 ◽

2019 ◽

Vol 12 (2) ◽

pp. 124-132

Author(s):

Nia Safitri ◽

Atria Martina ◽

Rodesia Mustika Roza

Keyword(s):

Fusarium Oxysporum ◽

Crop Production ◽

Plant Pathogens ◽

Pathogenic Fungi ◽

Culture Method ◽

Antagonistic Activity ◽

Cultivated Plants ◽

Dual Culture ◽

Trichoderma Sp

Tanaman budi daya merupakan tanaman yang sering diserang oleh cendawan pathogen, sehingga mengakibatkan penurunan populasi dan produksi tanaman. Pengendalian hayati dengan cendawan antagonis merupakan salah satu metode yang paling efektif dan lebih ramah lingkungan dalam menekan pertumbuhan patogen tanaman. Penelitian ini bertujuan untuk menguji aktivitas antagonis cendawan isolat lokal Riau terhadap beberapa cendawan patogen pada tanaman budi daya. Uji antagonis dilakukan secara in vitro dengan metode dual culture menggunakan lima belas cendawan isolat lokal Riau terhadap Fusarium oxysporum f.sp. lycopersici, Ganoderma philippii, G. boninense, Rigidoporus microporus dan Colletotrichum sansevieria. Hasil penelitian menunjukkan bahwa Trichoderma sp. PNE 4 memiliki aktivitas antagonis tertinggi dan isolat FER C1 serta isolat LLB07 hanya memiliki aktivitas antagonis yang tinggi dalam menekan pertumbuhan cendawan patogen. Trichoderma sp. PNE 4 mampu menghambat pertumbuhan miselium F. oxysporum sebesar 85,30%, G. Philippii (100%), G. boninense (100%), dan C. sansevieria (100%). Isolat FER C1 hanya menghambat R. Microporus (50,39%) dan isolat LLB07 menghambat G. philippii (52,20%). Trichoderma sp. PNE 4 merupakan cendawan uji yang terpilih sebagai cendawan antagonis, karena memiliki kemampuan daya hambat >70%.Abstract Cultivated plants are often attacked by pathogenic fungi resulting in a decline of population and crop production. Biocontrol with antagonistic fungi is one of the most effective and environmentally friendly methods in suppressing the growth of plant pathogens. This study aims to examine the antagonistic activity of local isolates fungi Riau against some pathogenic fungi on cultivated plants. The antagonistic test was performed in vitro by dual culture method using fifteen local isolates fungal Riau against Fusarium oxysporum f.sp. lycopersici, Ganoderma philippii, G. boninense, Rigidoporus microporus and Colletotrichum sansevieria. The results showed that Trichoderma sp. PNE 4 isolate exhibited highest activites and FER C1 and LLB07 isolates exhibited high activities suppressed the growth of the fungal pathogen. Trichoderma sp. PNE 4 isolate inhibited mycelial growth F. oxysporum (85.30%), G. philippii (100%), G. boninense (100%) and C. sansevieria (100%). FER C1 isolate only inhibited R. microporus (50.39%), and LLB07 isolate inhibited G. philippii (52.20%). Trichoderma sp. PNE 4 isolate is test isolates as fungal antagonistic.

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The activity of some Actinomycetes isolates in control of cucumber damping off disease caused by Rhizoctiona solani and Pythium sp.

Basrah Journal of Agricultural Sciences ◽

10.37077/25200860.2018.95 ◽

2018 ◽

Vol 31 (2) ◽

pp. 11-23

Author(s):

Lina K. Awad ◽

Mohammed A. Fayyadh

Keyword(s):

Date Palm ◽

Disease Incidence ◽

Streptomyces Griseus ◽

Inhibition Zone ◽

Antagonistic Activity ◽

Culture Technique ◽

Control Treatment ◽

Dual Culture ◽

Damping Off ◽

Accession Number

A 28 Actinomycetes isolates which collected from different environmental sources in Basra province were described as Gram positive and are characterized by producing branching hyphae. Two isolates were identified by molecular analysis of 16 S r RNA gene. Molecular identification confirmed that two isolates of Actinomycetes from soil had a similarity of 99% with Streptomyces griseus. The sequence has been deposited at NCBI with Gen bank accession number (NBRC 14886, AB 184627. 1). While the isolates of date palm roots was analogous to Brevibacterium celere and the sequence of this strain deposited at NCBI with Gen bank accession number (DQ164,K414744601). The dual culture technique showed that Actinomyces isolates 44 had high antagonistic activity against Rhizoctonia solani as inhibition zone reached 1.7 cm, in contrast to Actinomyces 24 and S. griseus which revealed a high antagonistic activity against Pythium sp. with inhibition zone reached 1.2 cm for both isolates. Pots experiment showed all Actinomyces isolates were significantly reduced cucumber seedling damping off caused by R. solani and Pythium sp. the disease incidence for R. solani damping off were reduced to 1.0% in actinomycetes 44, Actinomycetes 24 and B. celere treatment compared to 11.37 % in control treatment. Disease incidence at Pythium sp. damping off was reduced to 1.0% in Actinomycetes isolates compared with that in control treatment (4.33%). According to this study there is possibility for isolating Actinomycetes isolates which isolated from different environments sources have the ability for reducing cucumber damping off disease caused by R. solani and Pythium sp.

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Antagonism of Some Aquatic Hyphomycetes against Plant Pathogenic Fungi

The Scientific World JOURNAL ◽

10.1100/tsw.2010.80 ◽

2010 ◽

Vol 10 ◽

pp. 760-765 ◽

Cited By ~ 8

Author(s):

S. C. Sati ◽

P. Arya

Keyword(s):

Inhibitory Activity ◽

Radial Growth ◽

Pathogenic Fungi ◽

Antagonistic Activity ◽

Culture Technique ◽

Aquatic Hyphomycetes ◽

Colletotrichum Falcatum ◽

Plant Pathogenic Fungi ◽

Dual Culture ◽

Tilletia Indica

The antagonistic activity of five aquatic hyphomycetes, viz.,Heliscus lugdunensis,Tetrachaetum elegans,Tetracladium breve,T. marchalianum, andT. nainitalense, against seven plant pathogenic fungi was studied using a dual culture technique. Inhibitory activity of tested aquatic hyphomycetes was determined by measuring the radial growth of plant pathogenic fungi on dual culture plates.Tetrachaetum elegansshowed antagonistic activity againstColletotrichum falcatum,Fusarium oxysporum,Pyricularia oryzae,Sclerotium sclerotiorum, andTilletia indicaHeliscus lugdunensisshowed antagonism against only two plant pathogenic fungi,Rhizoctonia solaniandColletotrichum falcatum.Tetracladium breve,T. marchalianum, andT. nainitalenseshowed no response towards tested plant pathogenic fungi.

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