Phytochemical Analysis Of The Echinops Albicaulis Endemic Plant

Rindera graeca is a Greek endemic plant of the Boraginaceae family which has never been studied before. Consequently, this study attempted to phytochemically examine the aerial parts of this species. Nine phenolic secondary metabolites were identified, consisting of seven caffeic acid derivatives and two flavonol glucosides, namely rutin and quercetin-3-rutinoside-7-rhamnoside. These flavonoids, together with rosmarinic acid, were isolated via column chromatography and structurally determined through spectral analysis. Quercetin-3-rutinoside-7-rhamnoside is an unusual triglycoside, which is identified for the first time in Rindera genus and among Boraginaceae plants. This metabolite was further examined with thermal analysis and its 3D structure was simulated, revealing some intriguing information on its interaction with biological membrane models, which might have potential applications in microcirculation-related conditions. R. graeca was also analyzed for its pyrrolizidine alkaloids content, and it was found to contain echinatine together with echinatine N-oxide and rinderine N-oxide. Additionally, the total phenolic and flavonoid contents of R. graeca methanol extract were determined, along with free radical inhibition assays. High total phenolic content and almost complete inhibition at experimental doses at the free radical assays indicate a potent antioxidant profile for this plant. Overall, through phytochemical analysis and biological activity assays, insight was gained on an endemic Greek species of the little-studied Rindera genus, while its potential for further applications has been assessed.

Download Full-text

Phytochemical screening, antibacterial and antifungal activity of Pinda concanensis (Dalzell) P.K. Mukh. & Constance (Apiaceae) an endemic plant from Nasik district (M.S.) India

Annals of Plant Sciences ◽

10.21746/aps.2017.6.12.15 ◽

2017 ◽

Vol 6 (12) ◽

pp. 1886 ◽

Cited By ~ 1

Author(s):

Hemantkumar Atmaram Thakur

Keyword(s):

Antimicrobial Activity ◽

Medicinal Plants ◽

Economic Value ◽

Water Extract ◽

Diffusion Method ◽

Phytochemical Analysis ◽

Phytochemical Screening ◽

Endemic Plant ◽

Plant Parts ◽

Agar Disc

Medicinal plants are a source of great economic value all over the world. Various medicinal plants have been used for years in daily life to treat diseases. Present study illustrates the phytochemical screening and antimicrobial activity of Pinda concanensis (Dalzell) P.K. Mukh. & Constance. The selected plant Fruits were collected and powdered. The bioactive compounds were extracted by using Methanol, Ethanol, Petroleum ether and water in a soxhlet extractor. The antimicrobial activity was determined by using agar disc and well diffusion method. Acetone and chloroform extracts were mixed with 1ml diethyl sulfoxide (DMSO) and added into the well. The extract of Methanol with Pinda concanensis (Dalzell) P.K. Mukh. & Constance has shown maximum zone of inhibition against bacterial pathogens when compared to chloral and water extract. Phytochemical analysis reflected the antimicrobial activity of Pinda concanensis (Dalzell) P.K. Mukh. & Constance which is due to the presence of phytochemical compounds like alkaloids, terpenoids, steroids, flavonoids, Saponins, Phenolic compounds and tannins. The results of agar well diffusion method indicated the inhibition and it depends largely upon plant parts used and organism tested.

Download Full-text

Papaver Decaisnei: GC-MS Alkaloids Profiling, in Vitro Antioxidant, and Anticancer Activity

10.21203/rs.3.rs-1207324/v1 ◽

2022 ◽

Author(s):

Ahmed Aj.Jabbar ◽

Fuad Othman Abdullah ◽

Kamaran Kaiani Abdulrahman ◽

yaseen Galali ◽

Abdullah Sh. Sardar

Keyword(s):

Antioxidant Activity ◽

Radical Scavenging ◽

Reducing Power ◽

Phytochemical Analysis ◽

Endemic Plant ◽

Anticancer Activities ◽

Antioxidant Power ◽

Leaves And Roots ◽

Mcf 7

Abstract The Papaver L. plant have been well known as a source of pharmaceutically valuable alkaloids (noscapine, thebaine, codeine, roemerine, papaverine and morphine). The current study investigates the phytochemical, in-vitro antioxidant, and anticancer activities of papaver decaisnei, an endemic plant species to the flora of Kurdistan-Iraq. The chemical analysis of the methanolic (MeOH) extracts of flowers, leaves, and roots of papaver decaisnei were made by using gas chromatography-mass spectrophotometry (GC-MS), and the antioxidant activity evaluation done by radical scavenging [on 1,1-diphenyl-2-picrylhydrazyl (DPPH), 2,2j-azino-bis (3- ethylbenzothiazoline-6-sulfonic acid) (ABTS)], and reducing power [cupric reducing antioxidant capacity (CUPRAC), and ferric reducing antioxidant power (FRAP)] assays. The anticancer actions were presented as IC50 (inhibitory concentration at 50%) on human colorectal adenocarcinoma (Caco-2), mammary cancer cells (MCF-7), and human cervical carcinoma (HeLa) cells. The results of the phytochemical analysis showed 17, 19, and 22 chemical compounds for flowers, leaves, and roots of P. decaisnei, respectively. The prevalent organic compounds of P. decaisnei were alkaloids, phenolics, fatty acids, esters, and phytosterols, namely Roemerine (70.44%), Decarbomethoxytabersonine, 9,12,15-Octadecatrien-1-ol, Hexadecanoic acid, 6,8-Dioxa-3-thiabicyclo(3,2,1)octane 3,3-dioxide, and γ-Sitosterol. The antioxidant activity of plant organ extracts was within 39.1-143.5 μg/ml for DPPH and 123.12-276.4 μg/ml for ABTS assays, while, the FRAP and CUPRAC values ranged within 12.4- 34.3 and 42.6-75.8 μg/ml, respectively. The anticancer action of P.decaisnei organ extracts was found against all tested human cell lines (Caco-2, MCF-7, HeLa) with inhibitory concentrations (IC50) values between 125.3-388.4 μg/ml. The presented data on alkaloid contents and biological activity of P. decaisnei can serve a ground knowledge for the future biomedical synthesis and cancer research projects.

Download Full-text

PHYTOCHEMICAL EVALUATION OF ARTOCARPUS HIRSUTUS LAM. FRUIT EXTRACT: A POTENTIAL WILD ENDEMIC PLANT

International Journal of Current Pharmaceutical Research ◽

10.22159/ijcpr.2020v12i2.37488 ◽

2020 ◽

pp. 53-56

Author(s):

NEHA C. P. ◽

ABDUSSALAM A. K.

Keyword(s):

Ferulic Acid ◽

Gallic Acid ◽

Caffeic Acid ◽

High Performance ◽

Quantitative Estimation ◽

Appreciable Amount ◽

Phytochemical Analysis ◽

Endemic Plant ◽

Fruit Extract ◽

Qualitative And Quantitative

Objective: The present study includes the qualitative and quantitative estimation of phytochemical constituents and HPTLC (High-performance thin layer chromatography) analysis of phenolic acids in the fruit extract of Artocarpus hirsutus. Methods: Qualitative and quantitative analysis of Artocarpus hirsutus methanol fruit extract using standard methods. HPTLC analysis was performed by the CAMAG HPTLC system (Switzerland) for gallic acid, caffeic acid and ferulic acid determination. Results: The Preliminary phytochemical analysis revealed the presence of phytoconstituents such as flavonoids, phenols, tannins, steroids, glycosides, alkaloids, carbohydrate and protein. The methanol fruit extract has high carbohydrate content (267±0.02 mg/g FW). And good amount of protein, phenol, flavanoid were also found. The results of mineral studies shows that elements such as potassium (1.601%) and nitrogen (1.4%) were present in higher quantity. The result showed the presence of caffeic acid within peak 7 with an Rf (Retension factor) value 0.49, ferulic acid within peak 8 with an Rf value 0.60, and gallic acid within peak 5 with an Rf value 0.25. Conclusion: The results indicated that the Artocarpus hirsutus fruit contains an appreciable amount of bioactive compounds.

Download Full-text

In-vitro propagation and phytochemical profiling of a highly medicinal and endemic plant species of the Himalayan region (Saussurea costus)

Scientific Reports ◽

10.1038/s41598-021-03032-1 ◽

2021 ◽

Vol 11 (1) ◽

Author(s):

Ajmal Khan ◽

Azhar Hussain Shah ◽

Niaz Ali

Keyword(s):

Lateral Roots ◽

Multiple Shoot ◽

Garden Soil ◽

Phytochemical Analysis ◽

Endemic Plant ◽

Root Explants ◽

Leaf Petiole ◽

Full Strength ◽

Pharmacologically Active

AbstractEfficient protocols for callus induction and micro propagation of Saussurea costus (Falc.) Lipsch were developed and phytochemical diversity of wild and in-vitro propagated material was investigated. Brown and red compact callus was formed with frequency of 80–95%, 78–90%, 70–95% and 65–80% from seeds, leaf, petiole and root explants, respectively. MS media supplemented with BAP (2.0 mgL−1), NAA (1.0 mgL−1) and GA3 (0.25 mgL−1) best suited for multiple shoot buds initiation (82%), while maximum shoot length was formed on media with BAP (1.5 mgL−1), NAA (0.25 mgL−1) and Kinetin (0.5 mgL−1). Full strength media with IAA (0.5 mgL−1) along with IBA (0.5 mgL−1) resulted in early roots initiation. Similarly, maximum rooting (87.57%) and lateral roots formation (up to 6.76) was recorded on full strength media supplemented with BAP (0.5 mgL−1), IAA (0.5 mgL−1) and IBA (0.5 mgL−1). Survival rate of acclimatized plantlets in autoclaved garden soil, farmyard soil, and sand (2:1:1) was 87%. Phytochemical analysis revealed variations in biochemical contents i.e. maximum sugar (808.32 µM/ml), proline (48.14 mg/g), ascorbic acid (373.801 mM/g) and phenolic compounds (642.72 mgL−1) were recorded from callus cultured on different stress media. Nonetheless, highest flavenoids (59.892 mg/g) and anthocyanin contents (32.39 mg/kg) were observed in in-vitro propagated plants. GC–MS analysis of the callus ethyl acetate extracts revealed 24 different phytochemicals. The variability in secondary metabolites of both wild and propagated plants/callus is reported for the first time for this species. This study may provide a baseline for the conservation and sustainable utilization of S. costus with implications for isolation of unique and pharmacologically active compounds from callus or regenerated plantlets.

Download Full-text

Phytochemical Content, Antibacterial and Antioxidant Potential of Endemic Plant Anabasis aretioïdes Coss. & Moq. (Chenopodiaceae)

BioMed Research International ◽

10.1155/2020/6152932 ◽

2020 ◽

Vol 2020 ◽

pp. 1-16

Author(s):

Souad Senhaji ◽

Fatima Lamchouri ◽

Hamid Toufik

Keyword(s):

Antioxidant Activity ◽

Quantitative Analysis ◽

Ethyl Acetate ◽

Ethyl Acetate Extract ◽

Biological Activities ◽

Diffusion Method ◽

Total Phenolic ◽

Phytochemical Analysis ◽

Endemic Plant

This study aims to investigate the biological activities of endemic plant Anabasis aretioïdes harvested in eastern Morocco. Various extracts were prepared from aerial part by aqueous and organic extraction using a Soxhlet and cold maceration. Preliminary phytochemical analysis was carried out on the powder and on the different extracts by standard phytochemical tests, and was confirmed by a quantitative analysis based on the determination of total polyphenols and cathechic tannins. Antioxidant activity was evaluated in vitro by five methods: H2O2, DPPH, ABTS, FRAP, and RP, and the antibacterial activity was carried out by disc diffusion method and the determination of MIC and MBC. Phytochemical screening revealed the presence of cathechic tannins, saponins, and sterols and quantitative analysis showed that Ethyl acetate extract presented the high level of phenolic and cathechic tannins contents (46.79 ± 0.75 µg GAE/mg E and 46.46 ± 0.67 µg CE/mg E). A highest hydrogen peroxide activity was observed in aqueous macerated extract (7.84 ± 0.44%) and the macerated methanol extract has the highest rates for the other four antioxidant activity tests: It was able to reduce DPPH with an IC50 of 52.91 ± 0.24 µg/ml, the highest ABTS•+ radical scavenging capacity (48.99 ± 1.316 µg TE/mg E), it showed also the highest antioxidant activity by the FRAP and reducing power test (99.73 ± 3.570 µg TE/mg E and 72.176 ± 0.540 µg AAE/mg E). Antibacterial screening showed that the maximum zone of inhibition was noted for ethyl acetate extract against Staphylococcus aureus (13.5 mm). The lowest MIC value was obtained with methanolic and macerated methanolic extracts against Protéus mirabilis strain (MIC = 3.125 mg/ml). Principal component analysis showed that the four methods ABTS, DPPH, FRAP, and RP are highly correlated and a correlation between the antioxidant activity and the total phenolic contents of the extracts indicated that phenolic compounds were the dominant contributors to the antioxidant activity of the plant.

Download Full-text