VARIATION OF FUNCTIONAL PLATELET PARAMETERS DURING STORAGE AND DURING VENOUS OCCLUSSION MEASURED

1987 ◽  
Author(s):  
M Spannagl ◽  
G Valet ◽  
W Schramm
Keyword(s):  
Esterase Activity ◽  
Transmembrane Potential ◽  
Sodium Citrate ◽  
Bleeding Time ◽  
Blue Fluorescence ◽  
Platelet Volume ◽  
Blood Samples ◽  
The Mean ◽  
Variation Of Functional ◽  
Whole Blood Sample

Information on platelet function would be of great importance for many clinical situations in addition to platelet count and bleeding time. It was the purpose of this study to test Di0c6 (3,3-dihexyl-oxacarbocyanine: transmembrane potential), AO (acridine orange: granular content), and ADB (1,4-di-acetoxy-2,3-dicyanobenzene: intracellular esterase-activity and pH) stained platelets after 1 and 5 hours storage (anticoagulated with EDTA, Heparin and Sodium-Citrate) and 2, 6, 12 and 20 minutes after venous occlussion (immediately diluted in HEPES-Buffer (1:50)). A fresh whole blood sample diluted in Buffer served as control. All blood samples were gained from normal persons. Platelets were finally diluted 1:200 in HEPES buffered saline and stained. Cell volume, green and blue fluorescence were then measured in a Fluovo-Metricell-II flow cytometer.- The mean platelet volume increased to 111% (1 h) and 117% (5 h) of control during storage. The volume remained stable during venous occlussion. - The transmembrane potential (Di0c6) decreased to 52% of control after 5 hours storage. We saw an increase to 141% after 20 minutes venous occlussion.- The granular content (A0) decreased to 81% of control during storage. There was no variation during VOT.- Esterase activity (ADB) remained constant during storage and had the lowest coefficient of variation (CV = 51%). There was an increase to 132% after 20 minutes venous occlussion.- We saw most increase in volume and decrease in Di0c6 and A0 dye content after storage in EDTA compared to Citrate and Heparin.The present results show that the dyes of functional platelet parameters are sensitively picked up by flow cytometry. The methodology seems attractive for clinical purposes because measurements can be performed in diluted blood samples within less than five minutes after venipuncture.

1-Desamino-8-D-Arginine Vasopressin (DDAVP) Infusion in Type IIB von Willebrand’s Disease: Shortening of Bleeding Time and Induction of a Variable Pseudothrombocytopenia

1990 ◽  
Vol 64 (01) ◽  
pp. 117-120 ◽  
Author(s):  
Alessandra Casonato ◽  
M Teresa Sartori ◽  
Luigi de Marco ◽  
Antonio Girolami
Keyword(s):  
Monoclonal Antibody ◽  
Platelet Count ◽  
Arginine Vasopressin ◽  
Calcium Concentration ◽  
Sodium Citrate ◽  
Bleeding Time ◽  
Blood Collection ◽  
Von Willebrand's Disease ◽  
Blood Samples ◽  
Von Willebrand’S Disease

SummaryWe have investigated the effects of 1-desamino-8-D-arginine vasopressin (DDAVP) infusion on platelet count and bleeding time in 4 patients with type IIB von Willebrand’s disease (vWd). Three of four patients showed a normalization of the bleeding time within 1 h after the infusion, while bleeding time was not modified in the fourth. In accordance with the literature, thrombocytopenia was observed after DDAVP infusion, but this thrombocytopenia was due to the anticoagulants used for blood collection. In two patients (F. I., G. F.) no thrombocytopenia was observed when platelets were counted by fingerstick method but there was a 20% platelet decrease in blood samples collected in sodium citrate and a 50% decrease in samples collected in EDTA. Dramatic falls in platelet counts (70–95%) were observed in the additional two patients (C. A., D.Z.) after DDAVP infusion, when both sodium citrate or EDTA were used as anticoagulants. In the latter two patients there was also a 50% decrease in platelet count when the fingerstick method was used. The decrease in the patient’s platelet count in EDTA samples after DDAVP infusion could be prevented, in part, by the previous additions of an anti GPIb monoclonal antibody and an anti GPIIb-IIIa monoclonal antibody.Thus, the thrombocytopenia observed in the four IIB vWd patients studied after DDAVP infusion seems to be, at least partially, a pseudothrombocytopenia depending on the calcium concentration in the blood samples and the availability of GPIb and GPIIb-IIIa receptors. These findings and the normalization of the bleeding time observed in three of the four patients has led us to reconsider the possible use of DDAVP in the treatment of our IIB vWd patients.

Effects of biological variations on platelet count in healthy subjects in China

2004 ◽  
Vol 91 (02) ◽  
pp. 367-372 ◽  
Author(s):  
Jing Yang ◽  
Xiaojun Lu ◽  
Tokuhiro Okada ◽  
Tamiaki Kondo ◽  
Changgeng Ruan ◽  
...  
Keyword(s):  
Platelet Count ◽  
Mean Platelet Volume ◽  
Healthy Subjects ◽  
Bleeding Time ◽  
Platelet Volume ◽  
Platelet Counts ◽  
Normal Platelet Count ◽  
Normal Platelet ◽  
Low Platelet Count ◽  
The Mean

SummaryThe effects of biological variations on platelet counts were investigated in 694 healthy subjects aged 18 to 60 years living in three cities including Chengdu (Sichuan Province), Suzhou (Jiangsu Province) and Harbin (Heilongjang Province) in China. Platelet counts in healthy subjects were significantly lower in Chengdu (52∼202 X 109/L) and Suzhou (60∼259 X 109/L) than in Harbin (154∼348 X 109/L)(p <0.0001), but the mean platelet volume (MPV) determined concurrently was negatively correlated with platelet count, the MPV values were significantly higher in Chengdu (11.8∼15.6 fl) and Suzhou (10.9∼15.8 fl) than in Harbin (9.5∼12.9 fl) (p < 0.0001). Platelet counts were significantly higher in summer (73∼289 X 109/L) than in winter (52∼202 X 109/L) (p <0.0001), but the MPV values were lower in summer (11.2∼14.7 fl) than in winter (11.8∼15.6 fl) (p <0.05) in Chengdu. Platelet associated immunoglobulin (PA-IgG) in Chengdu was revealed to be significantly higher in the low platelet count group (<150 X 109/L, 13.5 ± 7.1 ng/107 PLT) than in the normal platelet count group (≥150 X 109/L, 8.3 ± 2.7 ng/107 PLT)(p <0.0001). Similar results were observed in Suzhou for the reticulated platelet ratio, which was significantly higher in the low platelet count group (19.5 ± 7.1%) than in the normal platelet count group (11.6 ± 2.7%)(p <0.01). The bleeding time in Chengdu showed a significantly longer time in the low platelet count group (8.6 ± 2.3 min) than in the normal platelet count group (6.0 ± 1.2 min)(p <0.01). With regard to the effects of lipids on platelet counts, the HDL values were significantly higher in the normal platelet count group (1.60 ± 0.76 mmol/L) than the low platelet count group (1.23 ± 0.31 mmol/L) (p <0.01); but no significant differences in cholesterol and triglycerides values between the normal and low platelet count groups (p >0.05) were recorded. These findings suggest that the platelet counts could be greatly influenced in healthy subjects by biological variations such as geographical, seasonal, and lipid variations.

scholarly journals A Novel Utility for an Old Method: The Leukocyte Adherence Inhibition Test Is an Easy Way to Detect the Immunoreactive Interference of the Collection Tube Anticoagulant on Cellular Immunoassays

2014 ◽  
Vol 2014 ◽  
pp. 1-6
Author(s):  
Celso Eduardo Olivier ◽  
Raquel Acácia Pereira Gonçalves dos Santos ◽  
Regiane Patussi Santos Lima ◽  
Daiana Guedes Pinto Argentão ◽  
Grayce Katlen Moreno da Silva ◽  
...  
Keyword(s):  
Sodium Citrate ◽  
Leukocyte Adherence ◽  
Blood Samples ◽  
Analytical Interference ◽  
Highly Sensitive ◽  
Leukocyte Adherence Inhibition ◽  
Collection Tube ◽  
Specific Antigens ◽  
The Mean ◽  
Adherence Inhibition

The leukocyte adherence inhibition (LAI) test was designed to evaluate the presence of cellular immunoreactivity against specific antigens and is highly sensitive to analytical interference. To compare the performance of the LAI test among plasmas obtained with different anticoagulants, 36 blood samples of allergic subjects were divided into three tubes with heparin, sodium citrate, and EDTA. The adherence was assessed with the hemocytometer technique. The mean adherence of each group was compared as well as the number of tests with inappropriate adherence (<25%). Of the 36 tests, there were 4, 9, and 21 with inappropriate adherence in the groups of heparin, citrate, and EDTA, respectively. Of these last, 5 samples also did not demonstrate proper adherence with sodium citrate and 1 sample did not show proper adherence with any anticoagulant. The anticoagulant in the collection tube can elicit a specific immune response, becoming an interferent for the LAI test. In this case, there is the possibility of redoing the test using another anticoagulant. Performed with different anticoagulants, the LAI test may be used as a preanalytical tool for choosing the less immunoreactive anticoagulant for more complex immunoassays.

The Decreased Circulating Platelet Mass and Its Relation to Bleeding Time in Chronic Renal Failure

1991 ◽  
Vol 65 (01) ◽  
pp. 011-014 ◽  
Author(s):  
E Michalak ◽  
B Walkowiak ◽  
M Paradowski ◽  
C S Cierniewski
Keyword(s):  
Renal Failure ◽  
Chronic Renal Failure ◽  
Electrical Impedance ◽  
Bleeding Time ◽  
Impedance Method ◽  
Bleeding Diathesis ◽  
Inverse Relation ◽  
Platelet Volume ◽  
The Mean ◽  
Platelet Mass

SummaryThe mean platelet volume, measured optically and by the electrical impedance method, was analysed in the patients with chronic renal failure. All parameters describing the circulating platelet biomass except the platelet volume heterogeneity were decreased in these patients. There is a strong inverse relation between the Ivy bleeding time and the platelet mass in the patient group.Our results indicate that the smaller platelets circulating in the patients with chronic renal failure may contribute to the uremic bleeding diathesis, and disturbed thrombocytopoiesis occurs in chronic renal failure.

scholarly journals Does varying the ingestion period of sodium citrate influence blood alkalosis and gastrointestinal symptoms?

PLoS ONE ◽  
2021 ◽  
Vol 16 (5) ◽  
pp. e0251808
Author(s):  
Charles S. Urwin ◽  
Rodney J. Snow ◽  
Liliana Orellana ◽  
Dominique Condo ◽  
Glenn D. Wadley ◽  
...  
Keyword(s):  
Experimental Design ◽  
Mixed Models ◽  
Sodium Citrate ◽  
Venous Blood ◽  
Gastrointestinal Symptoms ◽  
The Other ◽  
Strong Ion Difference ◽  
Blood Samples ◽  
Blood Ph ◽  
The Mean

Objectives To compare blood alkalosis, gastrointestinal symptoms and indicators of strong ion difference after ingestion of 500 mg.kg-1 BM sodium citrate over four different periods. Methods Sixteen healthy and active participants ingested 500 mg.kg-1 BM sodium citrate in gelatine capsules over a 15, 30, 45 or 60 min period using a randomized cross-over experimental design. Gastrointestinal symptoms questionnaires and venous blood samples were collected before ingestion, immediately post-ingestion, and every 30 min for 480 min post-ingestion. Blood samples were analysed for blood pH, [HCO3-], [Na+], [Cl-] and plasma [citrate]. Linear mixed models were used to estimate the effect of the ingestion protocols. Results For all treatments, blood [HCO3-] was significantly elevated above baseline for the entire 480 min post-ingestion period, and peak occurred 180 min post-ingestion. Blood [HCO3-] and pH were significantly elevated above baseline and not significantly below the peak between 150–270 min post-ingestion. Furthermore, blood pH and [HCO3-] were significantly lower for the 60 min ingestion period when compared to the other treatments. Gastrointestinal symptoms were minor for all treatments; the mean total session symptoms ratings (all times summed together) were between 9.8 and 11.6 from a maximum possible rating of 720. Conclusion Based on the findings of this investigation, sodium citrate should be ingested over a period of less than 60 min (15, 30 or 45 min), and completed 150–270 min before exercise.

Acute and Chronic Changes in Platelet Volume and Count After Cardiopulmonary Bypass Induced Thrombocytopenia in Man

1987 ◽  
Vol 57 (01) ◽  
pp. 55-58 ◽  
Author(s):  
J F Martin ◽  
T D Daniel ◽  
E A Trowbridge
Keyword(s):  
Platelet Count ◽  
Mean Platelet Volume ◽  
Artery Bypass ◽  
Bypass Graft ◽  
Control Group ◽  
Artery Bypass Graft ◽  
Platelet Volume ◽  
Young Males ◽  
The Mean

SummaryPatients undergoing surgery for coronary artery bypass graft or heart valve replacement had their platelet count and mean volume measured pre-operatively, immediately post-operatively and serially for up to 48 days after the surgical procedure. The mean pre-operative platelet count of 1.95 ± 0.11 × 1011/1 (n = 26) fell significantly to 1.35 ± 0.09 × 1011/1 immediately post-operatively (p <0.001) (n = 22), without a significant alteration in the mean platelet volume. The average platelet count rose to a maximum of 5.07 ± 0.66 × 1011/1 between days 14 and 17 after surgery while the average mean platelet volume fell from preparative and post-operative values of 7.25 ± 0.14 and 7.20 ± 0.14 fl respectively to a minimum of 6.16 ± 0.16 fl by day 20. Seven patients were followed for 32 days or longer after the operation. By this time they had achieved steady state thrombopoiesis and their average platelet count was 2.44 ± 0.33 × 1011/1, significantly higher than the pre-operative value (p <0.05), while their average mean platelet volume was 6.63 ± 0.21 fl, significantly lower than before surgery (p <0.001). The pre-operative values for the platelet volume and counts of these patients were significantly different from a control group of 32 young males, while the chronic post-operative values were not. These long term changes in platelet volume and count may reflect changes in the thrombopoietic control system secondary to the corrective surgery.

Influence of Certain Drugs on the Adhesiveness of Human, Rat, and Rabbit Blood Platelets in Vitro

1965 ◽  
Vol 13 (02) ◽  
pp. 428-438 ◽  
Author(s):  
K Reber ◽  
A Studer
Keyword(s):  
Sodium Citrate ◽  
Blood Platelets ◽  
Drug Application ◽  
Normal Range ◽  
Blood Samples ◽  
Serotonin Antagonist ◽  
Thrombocyte Count ◽  
Particle Count ◽  
Rabbit Blood

SummaryThis is a comparative study of the methods described by H. P. Wright and O’Brien for determining the adhesiveness of thrombocytes. An attempt is made to characterize and statistically correlate both techniques. With the aid of a Coulter Counter for thrombocyte counts, a normal range is presented for human, rat, and rabbit blood. Anticoagulants used are sodium citrate and Heparin.The influence of Cocaine and the Serotonin antagonist Ro 3-0837 was studied on these same substrates, to determine a pharmacological interference with results of either Wright’s test or O’Brien’s. Both drugs are found to induce a statistically significant increase in the “thrombocyte count” as compared to the corresponding controls. These effects are not real but to be attributed to an increase in particle count due to thrombocyte fragmentation as a consequence of drug application. There is no evidence for the claim that these drugs decrease the adhesiveness of thrombocytes.Numerical results of both tests often show a high and statistically significant correlation, especially following the addition of Ro 3-0837. Such is not true of individual blood samples to which no drug has been added. Evidentally, both tests are not specific for the same characteristic of normal blood platelets. But, when Ro 3-0837 is added, the breakdown of unstable platelets is induced; and the corresponding increase in count of thrombocyte fragments is expressed by both tests in the same fashion.

Association of Retained Fetal Membranes with Haematological Profile in Crossbred Cows

2017 ◽  
Vol 12 (3) ◽  
Author(s):  
K. Rokde ◽  
S. Kumar ◽  
A. Bhardwaz ◽  
S. S. Mahour ◽  
S. P. Nema ◽  
...  
Keyword(s):  
Eosinophil Count ◽  
Jugular Vein ◽  
Dairy Farm ◽  
Fetal Membranes ◽  
Monocyte Count ◽  
Blood Samples ◽  
Mean Values ◽  
Crossbred Cows ◽  
Haematological Profile ◽  
The Mean

This study was carried out on clinical cases of retained fetal membranes in crossbred cows presented at College Clinics and College dairy farm and from Villages in and around Mhow. The blood samples were collected from jugular vein just before 12 hr. postpartum and on 7th day postpartum. Haematological profile revealed that the mean values of haemoglobin, neutrophil and monocyte count after 12 hrs and 7th day postpartum were significantly lower and lymphocyte count was significantly higher in RFM cows (n=18) than normally calved cows (n=6). The differences in mean TLC, eosinophil and basophil counts were non-significant at 12 hrs postpartum, however on 7th day postpartum the TLC and eosinophil count were significantly higher and basophil count was non-significantly different in RFM cows than the normally calved cows.

PS-095a The Mean Platelet Volume In Infants With Meconium Stained Amniotic Fluid

2014 ◽  
Vol 99 (Suppl 2) ◽  
pp. A144.4-A145
Author(s):  
M Tekin ◽  
C Konca ◽  
Z Kahramaner ◽  
A Erdemir ◽  
A Gulyuz ◽  
...  
Keyword(s):  
Amniotic Fluid ◽  
Mean Platelet Volume ◽  
Platelet Volume ◽  
The Mean ◽  
Meconium Stained Amniotic Fluid

scholarly journals Handling Associated with Drenching Does Not Impact Survival and General Health of Low Birth Weight Piglets

Animals ◽  
2021 ◽  
Vol 11 (2) ◽  
pp. 404
Author(s):  
Kevin Van Tichelen ◽  
Sara Prims ◽  
Miriam Ayuso ◽  
Céline Van Kerschaver ◽  
Mario Vandaele ◽  
...  
Keyword(s):  
Birth Weight ◽  
Low Birth Weight ◽  
Significant Risk ◽  
Skin Lesions ◽  
Additional Stress ◽  
Bioactive Substances ◽  
Blood Samples ◽  
Panel Test ◽  
Esterified Fatty Acids ◽  
The Mean

The increase in litter sizes in recent years has resulted in more low birth weight (LBW) piglets, accompanied by a higher mortality. A potential intervention to overcome this is drenching bioactive substances. However, if the act of drenching provokes additional stress in LBW piglets, it might counteract the supplement’s effect and be detrimental for the piglet’s survival. To study the effect of the drenching act, piglets from 67 sows were weighed within 4 h after birth. The mean litter birth weight (MLBW) and standard deviation (SD) were calculated. LBW piglets (n = 76) were defined as weighing between (MLBW-1*SD) and (MLBW-2.5*SD). They were randomly allocated to two treatments: “sham” (conducting the act of drenching by inserting an empty 2.5 mL syringe in the mouth during 20 s, once a day, d1 till d7; n = 37) or “no treatment” (no handling; n = 39). On day 1, 3, 9, 24 and 38, piglets were weighed and scored for skin lesions. Blood samples were collected on day 9 and 38 and analyzed to determine glucose, non-esterified fatty acids (NEFA), urea, immunoglobulin G (IgG), insulin-like growth factor 1 (IGF-1) and a standard blood panel test. There was no difference between sham drenched and untreated piglets regarding any of the parameters. In conclusion, this study showed that drenching does not impose a significant risk to LBW piglets and can be applied safely during the first 7 days after birth.

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