Acute Hematological and Inflammatory Responses to High-intensity Exercise Tests: Impact of Duration and Mode of Exercise

2017 ◽  
Vol 38 (07) ◽  
pp. 551-559 ◽  
Author(s):  
Luciele Minuzzi ◽  
Humberto Carvalho ◽  
Diego Brunelli ◽  
Fatima Rosado ◽  
Cláudia Cavaglieri ◽  
...  
Keyword(s):  
Energy Expenditure ◽  
High Intensity ◽  
Present Finding ◽  
Inflammatory Responses ◽  
Hematological Parameters ◽  
Cycle Ergometer ◽  
High Intensity Exercise ◽  
Exercise Protocol ◽  
Exercise Tests ◽  
Repeated Sprints

AbstractThe purpose of this study was to investigate the hematological and inflammatory responses to 4 maximal high-intensity protocols, considering energy expenditure in each test. 9 healthy volunteers performed 4 high-intensity exercise tests of short [Wingate (WANT); Repeated-sprints (RSA)] and long durations [Continuous VO2 test (VCONT); intermittent VO2 test (VINT)] in a cycle-ergometer, until exhaustion. Hematological parameters and IL-6, IL-10 and creatine kinase (CK) levels were determined before (PRE), POST, 30 min, 1, 2, 12 and 24 h after the end of the protocols. Additionally, energy expenditure was determined. Leucocytes, erythrocytes and lymphocytes increased at POST and returned to PRE values at 30 min for all protocols. Lymphocytes had a second decreased at 2 h and granulocytes increased at 2 h when compared to PRE. Both variables returned to PRE values between 12–24 h into recovery. The magnitude of response for IL-6 was greater in VINT and for IL-10 in VCONT. There was no association of energy expenditure within each exercise protocol with the pattern of IL-6, IL-10 and CK responses to the exercise protocols. The present finding support that similar responses after continuous or intermittent acute protocols are observed when exercises are performed to volitional failure, regardless of the duration and mode of exercise.

Influence of endurance training on muscle [PCr] kinetics during high-intensity exercise

2007 ◽  
Vol 293 (1) ◽  
pp. R392-R401 ◽  
Author(s):  
Andrew M. Jones ◽  
Daryl P. Wilkerson ◽  
Nicolas J. Berger ◽  
Jonathan Fulford
Keyword(s):  
Endurance Training ◽  
High Intensity ◽  
Slow Component ◽  
Knee Extension ◽  
High Intensity Exercise ◽  
Whole Body ◽  
Time To Exhaustion ◽  
Exercise Tests ◽  
Before And After ◽  
Knee Extension Exercise

We hypothesized that a period of endurance training would result in a speeding of muscle phosphocreatine concentration ([PCr]) kinetics over the fundamental phase of the response and a reduction in the amplitude of the [PCr] slow component during high-intensity exercise. Six male subjects (age 26 ± 5 yr) completed 5 wk of single-legged knee-extension exercise training with the alternate leg serving as a control. Before and after the intervention period, the subjects completed incremental and high-intensity step exercise tests of 6-min duration with both legs separately inside the bore of a whole-body magnetic resonance spectrometer. The time-to-exhaustion during incremental exercise was not changed in the control leg [preintervention group (PRE): 19.4 ± 2.3 min vs. postintervention group (POST): 19.4 ± 1.9 min] but was significantly increased in the trained leg (PRE: 19.6 ± 1.6 min vs. POST: 22.0 ± 2.2 min; P < 0.05). During step exercise, there were no significant changes in the control leg, but end-exercise pH and [PCr] were higher after vs. before training. The time constant for the [PCr] kinetics over the fundamental exponential region of the response was not significantly altered in either the control leg (PRE: 40 ± 13 s vs. POST: 43 ± 10 s) or the trained leg (PRE: 38 ± 8 s vs. POST: 40 ± 12 s). However, the amplitude of the [PCr] slow component was significantly reduced in the trained leg (PRE: 15 ± 7 vs. POST: 7 ± 7% change in [PCr]; P < 0.05) with there being no change in the control leg (PRE: 13 ± 8 vs. POST: 12 ± 10% change in [PCr]). The attenuation of the [PCr] slow component might be mechanistically linked with enhanced exercise tolerance following endurance training.

scholarly journals A Short Bout of High-intensity Exercise Alters Ipsilesional Motor Cortical Excitability Post-stroke

2019 ◽  
Author(s):  
Xin Li ◽  
Charalambos C. Charalambous ◽  
Darcy S. Reisman ◽  
Susanne M. Morton
Keyword(s):  
High Intensity ◽  
Acute Exercise ◽  
Cortical Excitability ◽  
Motor Evoked Potentials ◽  
High Intensity Exercise ◽  
Exercise Protocol ◽  
Post Stroke ◽  
Motor Cortical Excitability ◽  
Motor Cortical ◽  
Lactate Levels

AbstractBackgroundAcute exercise can increase motor cortical excitability and enhance motor learning in healthy individuals, an effect known as exercise priming. Whether it has the same effects in people with stroke is unclear.ObjectivesThe objective of this study was to investigate whether a short, clinically-feasible high-intensity exercise protocol can increase motor cortical excitability in non-exercised muscles of chronic stroke survivors.MethodsThirteen participants with chronic, unilateral stroke participated in two sessions, at least one week apart, in a crossover design. In each session, they underwent either high-intensity lower extremity exercise or quiet rest. Motor cortical excitability of the extensor carpi radialis muscles was measured bilaterally with transcranial magnetic stimulation before and immediately after either exercise or rest. Motor cortical excitability changes (post-exercise or rest measures normalized to pre-test measures) were compared between exercise vs. rest conditions.ResultsAll participants were able to reach the target high-intensity exercise level. Blood lactate levels increased significantly after exercise (p < 0.001, d = 2.85). Resting motor evoked potentials from the lesioned hemisphere increased after exercise compared to the rest condition (p = 0.046, d = 2.76), but this was not the case for the non-lesioned hemisphere (p = 0.406, d = 0.25).ConclusionsHigh-intensity exercise can increase lesioned hemisphere motor cortical excitability in a non-exercised muscle post-stroke. Our short and clinically-feasible exercise protocol shows promise as a potential priming method in stroke rehabilitation.

scholarly journals Defining the contribution of skeletal muscle pyruvate dehydrogenase α1 to exercise performance and insulin action

2018 ◽  
Vol 315 (5) ◽  
pp. E1034-E1045 ◽  
Author(s):  
Kristoffer Svensson ◽  
Jessica R. Dent ◽  
Shahriar Tahvilian ◽  
Vitor F. Martins ◽  
Abha Sathe ◽  
...  
Keyword(s):  
Skeletal Muscle ◽  
Insulin Sensitivity ◽  
Energy Expenditure ◽  
Exercise Performance ◽  
Pyruvate Dehydrogenase ◽  
High Intensity ◽  
High Intensity Exercise ◽  
Low Intensity ◽  
Low Intensity Exercise ◽  
Muscle Contractile

The pyruvate dehydrogenase complex (PDC) converts pyruvate to acetyl-CoA and is an important control point for carbohydrate (CHO) oxidation. However, the importance of the PDC and CHO oxidation to muscle metabolism and exercise performance, particularly during prolonged or high-intensity exercise, has not been fully defined especially in mature skeletal muscle. To this end, we determined whether skeletal muscle-specific loss of pyruvate dehydrogenase alpha 1 ( Pdha1), which is a critical subunit of the PDC, impacts resting energy metabolism, exercise performance, or metabolic adaptation to high-fat diet (HFD) feeding. For this, we generated a tamoxifen (TMX)-inducible Pdha1 knockout (PDHmKO) mouse, in which PDC activity is temporally and specifically ablated in adult skeletal muscle. We assessed energy expenditure, ex vivo muscle contractile performance, and endurance exercise capacity in PDHmKO mice and wild-type (WT) littermates. Additionally, we studied glucose homeostasis and insulin sensitivity in muscle after 12 wk of HFD feeding. TMX administration largely ablated PDHα in skeletal muscle of adult PDHmKO mice but did not impact energy expenditure, muscle contractile function, or low-intensity exercise performance. Additionally, there were no differences in muscle insulin sensitivity or body composition in PDHmKO mice fed a control or HFD, as compared with WT mice. However, exercise capacity during high-intensity exercise was severely impaired in PDHmKO mice, in parallel with a large increase in plasma lactate concentration. In conclusion, although skeletal muscle PDC is not a major contributor to resting energy expenditure or long-duration, low-intensity exercise performance, it is necessary for optimal performance during high-intensity exercise.

Pre-exercise β-hydroxy-β-methylbutyrate free-acid supplementation improves work capacity recovery: a randomized, double-blinded, placebo-controlled study

2018 ◽  
Vol 43 (7) ◽  
pp. 691-696 ◽  
Author(s):  
Ana Luiza Matias Correia ◽  
Filipe Dinato de Lima ◽  
Martim Bottaro ◽  
Amilton Vieira ◽  
Andrew Correa da Fonseca ◽  
...  
Keyword(s):  
Single Dose ◽  
High Intensity ◽  
Time Course ◽  
Free Acid ◽  
Exercise Bout ◽  
Work Capacity ◽  
High Intensity Exercise ◽  
Controlled Study ◽  
Exercise Protocol ◽  
Young Males

The purpose of this study was to investigate the effects of a single-dose of β-hydroxy-β-methylbutyrate free acid (HMB-FA) supplementation on muscle recovery after a high-intensity exercise bout. Twenty-three trained young males were randomly assigned to receive either a single-dose supplementation of 3 g of HMB-FA (n = 12; age, 22.8 ± 3.0 years) or placebo (PLA; n = 11; age, 22.9 ± 3.1 years). A muscle damage protocol was applied 60 min after supplementation, and consisted of 7 sets of 20 drop jumps from a 60-cm box with 2-min rest intervals between sets. Muscle swelling, countermovement jump (CMJ), maximal voluntary isometric torque (MVIT), and work capacity (WC) were measured before, immediately after, and 24, 48, and 72 h after the exercise protocol. Muscle swelling, CMJ, and MVIT changed similarly in both groups after the exercise protocol (p < 0.001), but returned to pre-exercise levels after 24 h in both groups. WC decreased similarly in both groups after the exercise protocol (p < 0.01). For HMB-FA, WC returned to pre-exercise level 24 h after exercise protocol. However, for PLA, WC did not return to pre-exercise level even 72 h after the exercise protocol. In summary, a single-dose of HMB-FA supplementation improved WC recovery after a high-intensity exercise bout. However, HMB-FA did not affect the time-course of muscle swelling, MVIT, and CMJ recovery.

scholarly journals Complex I is bypassed during high intensity exercise

2019 ◽  
Vol 10 (1) ◽  
Author(s):  
Avlant Nilsson ◽  
Elias Björnson ◽  
Mikael Flockhart ◽  
Filip J. Larsen ◽  
Jens Nielsen
Keyword(s):  
High Intensity ◽  
Complex I ◽  
Atp Synthesis ◽  
Metabolic Model ◽  
High Intensity Exercise ◽  
Whole Body ◽  
Synthesis Rate ◽  
Proteomics Data ◽  
Exercise Tests

Abstract Human muscles are tailored towards ATP synthesis. When exercising at high work rates muscles convert glucose to lactate, which is less nutrient efficient than respiration. There is hence a trade-off between endurance and power. Metabolic models have been developed to study how limited catalytic capacity of enzymes affects ATP synthesis. Here we integrate an enzyme-constrained metabolic model with proteomics data from muscle fibers. We find that ATP synthesis is constrained by several enzymes. A metabolic bypass of mitochondrial complex I is found to increase the ATP synthesis rate per gram of protein compared to full respiration. To test if this metabolic mode occurs in vivo, we conduct a high resolved incremental exercise tests for five subjects. Their gas exchange at different work rates is accurately reproduced by a whole-body metabolic model incorporating complex I bypass. The study therefore shows how proteome allocation influences metabolism during high intensity exercise.

Failure of Glycine-Arginine-α-Ketoisocaproic Acid to Improve High-Intensity Exercise Performance in Trained Cyclists

2011 ◽  
Vol 21 (1) ◽  
pp. 33-39 ◽  
Author(s):  
Lukas Beis ◽  
Yaser Mohammad ◽  
Chris Easton ◽  
Yannis P. Pitsiladis
Keyword(s):  
Exercise Performance ◽  
High Intensity ◽  
Perceived Exertion ◽  
Cycle Ergometer ◽  
High Intensity Exercise ◽  
Ratings Of Perceived Exertion ◽  
Mean Power ◽  
Double Blind ◽  
Oral Supplementation ◽  
Experimental Trials

Oral supplementation with glycine-arginine-α-ketoisocaproic acid (GAKIC) has previously been shown to improve exhaustive high-intensity exercise performance. There are no controlled studies involving GAKIC supplementation in well-trained subjects. The aim of the current study was to examine the effects of GAKIC supplementation on fatigue during high-intensity, repeated cycle sprints in trained cyclists. After at least 2 familiarization trials, 10 well-trained male cyclists completed 2 supramaximal sprint tests each involving 10 sprints of 10 s separated by 50-s rest intervals on an electrically braked cycle ergometer. Subjects ingested 11.2 g of GAKIC or placebo (Pl) during a period of 45 min before the 2 experimental trials, administered in a randomized and double-blind fashion. Peak power declined from the 1st sprint (M ± SD; Pl 1,332 ± 307 W, GAKIC 1,367 ± 342 W) to the 10th sprint (Pl 1,091 ± 229 W, GAKIC 1,061 ± 272 W) and did not differ between conditions (p = .88). Mean power declined from the 1st sprint (Pl 892 ± 151 W, GAKIC 892 ± 153 W) to the 10th sprint (Pl 766 ± 120 W, GAKIC 752 ± 138 W) and did not differ between conditions (p = .96). The fatigue index remained at ~38% throughout the series of sprints and did not differ between conditions (p = .99). Heart rate and ratings of perceived exertion increased from the 1st sprint to the 10th sprint and did not differ between conditions (p = .11 and p = .83, respectively). In contrast to previous studies in untrained individuals, these results suggest that GAKIC has no ergogenic effect on repeated bouts of high-intensity exercise in trained individuals.

Water Intake Accelerates Parasympathetic Reactivation After High-Intensity Exercise

2014 ◽  
Vol 24 (5) ◽  
pp. 489-496 ◽  
Author(s):  
Tiago Peçanha ◽  
Marcelle Paula-Ribeiro ◽  
Edson Campana-Rezende ◽  
Rhenan Bartels ◽  
João Carlos Bouzas Marins ◽  
...  
Keyword(s):  
Heart Rate ◽  
Water Intake ◽  
High Intensity ◽  
Cycle Ergometer ◽  
High Intensity Exercise ◽  
Exercise Session ◽  
Exponential Fitting ◽  
Maximal Heart Rate ◽  
Parasympathetic Reactivation ◽  
Cardiovascular Benefit

It has been shown that water intake (WI) improves postexercise parasympathetic recovery after moderateintensity exercise session. However, the potential cardiovascular benefit promoted by WI has not been investigated after high-intensity exercise.Purpose:To assess the effects of WI on post high-intensity parasympathetic recovery.Methods:Twelve recreationally active young men participated in the study (22 ± 1.4 years, 24.1 ± 1.6 kg.m−2). The experimental protocol consisted of two visits to the laboratory. Each visit consisted in the completion of a 30-min high-intensity [~80% of maximal heart rate (HR)] cycle ergometer aerobic session performing randomly the WI or control (CON, no water consumption) intervention at the end of the exercise. HR and RR intervals (RRi) were continuously recorded by a heart rate monitor before, during and after the exercise. Differences in HR recovery [e.g., absolute heart rate decrement after 1 min of recovery (HRR60s) and time-constant of the first order exponential fitting curve of the HRR (HRRτ)] and in postexercise vagalrelated heart rate variability (HRV) indexes (rMSSD30s, rMSSD, pNN50, SD1 and HF) were calculated and compared for WI and CON.Results:A similar HR recovery and an increased postexercise HRV [SD1 = 9.4 ± 5.9 vs. 6.0 ± 3.9 millisecond, HF(ln) = 3.6 ± 1.4 vs. 2.4 ± 1.3 millisecond2, for WI and CON, respectively; p < .05] was observed in WI compared with CON.Conclusion:The results suggest that WI accelerates the postexercise parasympathetic reactivation after high-intensity exercise. Such outcome reveals an important cardioprotective effect of WI.

Force, speed, and oxygen consumption in Thoroughbred and draft horses

1998 ◽  
Vol 84 (6) ◽  
pp. 2052-2059 ◽  
Author(s):  
U. Silke Birlenbach Potard ◽  
David E. Leith ◽  
M. Roger Fedde
Keyword(s):  
Body Weight ◽  
Oxygen Consumption ◽  
High Intensity ◽  
High Intensity Exercise ◽  
Peak Efficiency ◽  
Exercise Tests ◽  
Progressive Exercise ◽  
Draft Force ◽  
External Power ◽  
Locomotor Muscles

Thoroughbred (TB) and draft horses (DH) have long been selected for tasks of very different intensities and force-speed relationships. To study their adaptations, we measured O2 consumption and related variables in three TB and four DH during progressive exercise tests on a level treadmill. The horses exerted a draft force of 0, 5, 10, 15, or 20% of their body weight at speeds that increased by 2 m/s every 3 min until they could not maintain that speed. We found that TB could exert the same draft forces as DH and, at each force, TB achieved about twice the speed, twice the external power, and twice the O2 consumption as DH; thus the two breeds had the same gross efficiencies. We also found maximal O2 consumption of TB to be about twice that of DH (134 vs. 72 ml ⋅ kg−1 ⋅ min−1, respectively), suggesting adaptations to high-intensity exercise. Peak efficiency was reached at lower speeds in DH than in TB, suggesting adaptations to high-force, low-speed exercise. These differences between TB and DH in force-speed and aerobic capacities and in speed for peak efficiency likely reflect different contraction velocities in locomotor muscles.

scholarly journals Modified Total-Body Recumbent Stepper Exercise Test for Assessing Peak Oxygen Consumption in People With Chronic Stroke

2008 ◽  
Vol 88 (10) ◽  
pp. 1188-1195 ◽  
Author(s):  
Sandra A Billinger ◽  
Benjamin Y Tseng ◽  
Patricia M Kluding
Keyword(s):  
Oxygen Consumption ◽  
Exercise Test ◽  
Health Care Professionals ◽  
Cycle Ergometer ◽  
Peak Oxygen Consumption ◽  
Exercise Protocol ◽  
Exercise Tests ◽  
Cycle Ergometer Exercise ◽  
Ergometer Exercise ◽  
Total Body

Background Assessment of peak oxygen consumption (V̇o2peak) using traditional modes of testing such as treadmill or cycle ergometer can be difficult in individuals with stroke due to balance deficits, gait impairments, or decreased coordination. Objective The purpose of this study was to quantitatively assess the validity and feasibility of a modified exercise test using a total-body recumbent stepper (mTBRS-XT) in individuals after stroke. Design A within-subject design, with a sample of convenience, was used. Participants Eleven participants (7 male, 4 female) with a mean of 40.1 months (SD=32.7) after stroke, a mean age of 60.9 years (SD=12.0), and mild to severe lower-extremity Fugl-Myer test scores (range=13–34) completed the study. Methods Participants performed 2 maximal-effort graded exercise tests on separate days using the mTBRS-XT and a cycle ergometer exercise protocol to assess cardiorespiratory fitness. Measurements of V̇o2peak and peak heart rate (peak HR) were obtained during both tests. Results A strong relationship existed between the mTBRS-XT and the cycle ergometer exercise test for V̇o2peak and peak HR (r=.91 and .89, respectively). Mean V̇o2peak was significantly higher for the mTBRS-XT (16.6 mL×kg−1×min−1[SD=4.5]) compared with the cycle ergometer exercise protocol (15.4 mL×kg−1×min−1 [SD=4.5]). All participants performed the mTBRS-XT. One individual with severe stroke was unable to pedal the cycle ergometer. No significant adverse events occurred. Conclusion The mTBRS-XT may be a safe, feasible, and valid exercise test to obtain measurements of V̇o2peak in people with stroke. Health care professionals may use the mTBRS-XT to prescribe aerobic exercise based on V̇o2peak values for individuals with mild to severe deficits after stroke.

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