Anti-inflammatory, Antipyretic, and Antinociceptive Effects of a Cressa cretica Aqueous Extract

Planta Medica ◽  
2017 ◽  
Vol 83 (17) ◽  
pp. 1313-1320 ◽  
Author(s):  
Heba Abdallah ◽  
Abdelsamed Elshamy ◽  
Abd El Gendy ◽  
Ahmed Abd El-Gawad ◽  
Elsayed Omer ◽  
...  
Keyword(s):  
Aqueous Extract ◽  
Wide Spectrum ◽  
Chemical Characterization ◽  
Control Group ◽  
Standard Drug ◽  
Whole Plant ◽  
Antinociceptive Effects ◽  
Anti Inflammatory

Abstract Cressa cretica is a widely grown halophytic plant traditionally used for the treatment of different ailments. Previous investigations reported its biological activity on a wide spectrum of diseases. In this study, in vivo antinociceptive, anti-inflammatory, and antipyretic activities of C. cretica aqueous extract whole plant were evaluated. In addition, the total polyphenol content, the total flavonoid content, and the chemical characterization of the extract were performed. C. cretica showed writhing inhibition in acetic acid-induced peripheral nociception of 43 and 48 % at doses of 50 and 100 mg/kg, respectively. The same doses increased latency time in a hot plate model of central analgesia by 66 and 78 % compared to the control group, respectively. The acute anti-inflammatory effect of the extract was explored in the carrageenan-induced rat hind paw test. The inhibition of paw volume was better than that of the standard drug indomethacin. C. cretica significantly decreased rectal temperature in the rats injected with Brewerʼs yeast. C. cretica aqueous extract showed both central and peripheral antinociceptive activities and was effective as an anti-inflammatory and antipyretic. Phenolic compounds, including chlorogenic acids and flavonol glycosides, were identified by HPLC-PDA-ESI-MS techniques. These findings indicate the medicinal importance of this traditionally used plant as a therapeutic remedy for different ailments.

scholarly journals Phenolic Content, Antioxidant Activity, Anti-Inflammatory Potential, and Acute Toxicity Study of Thymus leptobotrys Murb. Extracts

2020 ◽  
Vol 2020 ◽  
pp. 1-7
Author(s):  
Asmaa Oubihi ◽  
Hanae Hosni ◽  
Issmail Nounah ◽  
Abdessamad Ettouil ◽  
Hicham Harhar ◽  
...  
Keyword(s):  
Antioxidant Activity ◽  
Female Rats ◽  
Trolox Equivalent Antioxidant Capacity ◽  
Control Group ◽  
Standard Drug ◽  
Methanolic Extracts ◽  
Anti Inflammatory ◽  
Inflammatory Effect

Thymus leptobotrys is a medicinal plant belonging to the Lamiaceae family, endemic in Morocco, and used in traditional medicine. The present work aims to study the phenolic compounds, the antioxidant activity, the anti-inflammatory effect, and the toxicity of two ethanolic and methanolic extracts of Thymus leptobotrys aerial part. The yield of the methanolic extraction (22.2%) is higher than that of the ethanolic extraction (15.8%) and is characterized by higher contents of polyphenols 243.08 mg/g GAE (mg/g of gallic acid), flavonoids 179.28 mg/g RE (mg/g of rutin), and tannins 39.31 mg/g CE (mg/g of catechin). The in vitro measurement of antioxidant activity with the 2,2-diphenyl-1-picrylhydrazyl (DPPH) radical reduction test and Trolox equivalent antioxidant capacity (TEAC) test demonstrates the higher performance of the methanolic extract. The evaluation of the anti-inflammatory effect in vivo on adult Wistar female rats leads to a very significant decrease in the inflammation of the edema compared to the standard drug (indomethacin) and the control group. The toxicity test reveals that both extracts showed no toxicity within an LD50 above 2000 mg/kg body weight of the rats.

Chemical Characterization and In Vivo Anti-Inflammatory Activities of Actinidia callosa var. ephippioides via Suppression of Proinflammatory Cytokines

2013 ◽  
Vol 41 (02) ◽  
pp. 405-423 ◽  
Author(s):  
Jung-Chun Liao ◽  
Shyh-Shyun Huang ◽  
Jeng-Shyan Deng ◽  
Chao-Ying Lee ◽  
Ying-Chih Lin ◽  
...  
Keyword(s):  
Chemical Characterization ◽  
Ethyl Acetate Fraction ◽  
Paw Edema ◽  
Inflammatory Mechanism ◽  
Tnf Α ◽  
Anti Inflammatory ◽  
Factor Α ◽  
Oxide Synthase

Actinidia callosa var. ephippioides (ACE) has been widely used to treat anti-pyretic, antinociceptive, anti-inflammation, abdominal pain and fever in Taiwan. This study aims to determine the mechanism of anti-inflammatory activities of ethyl acetate fraction of ACE (EA-ACE) using a model of λ-carrageenan (Carr)-induced paw edema in mouse model. In HPLC analysis, chemical characterization of EA-ACE was established. In order to investigate the anti-inflammatory mechanism of EA-ACE, we have detected the activities of catalase (CAT), superoxide dismutase (SOD), and glutathione peroxidase (GPx) and the levels of malondialdehyde (MDA) in the paw edema. Serum NO, tumor necrosis factor α (TNF-α), and interleukin-1β (IL-1β) were evaluated. Chemical characterization from HPLC indicated that EA-ACE contains betulinic acid, ursolic acid and oleanolic acid. In the anti-inflammatory test, EA-ACE decreased the paw edema after Carr administration, increased the activities of CAT, SOD, and GPx and decreased the MDA level in the edema paw at the 5th hr after Carr injection. EA-ACE affects the serum NO, TNF-α, and IL-1β levels at the 5th hr after Carr injection. EA-ACE decreased Carr-induced inducible nitric oxide synthase (iNOS) and cyclooxygenase-2 (COX-2) expressions by Western blotting. Actinidia callosa var. ephippioides have the potential to provide a therapeutic approach to inflammation-associated disorders.

scholarly journals Evaluation of the curative role, Anti-inflammatory and anti-oxidative activity of some dietary spices on carrageenan induced paw edema in albino Wistar rats

2020 ◽  
Vol 10 (5-s) ◽  
pp. 90-96
Author(s):  
Khaoula Bouaoud ◽  
Noreddine Menadi ◽  
Mohammed Zairi ◽  
Sofiane Bouazza ◽  
Hadjer Bekhadda ◽  
...  
Keyword(s):  
Aqueous Extract ◽  
Wistar Rats ◽  
Red Pepper ◽  
Total Phenolic ◽  
Control Group ◽  
Paw Edema ◽  
Standard Drug ◽  
Reference Drug ◽  
Anti Inflammatory ◽  
Dietary Spices

Background: Inflammation is a major burning problem worldwide and billions of individuals are affected. Some of the spices used daily in Algerian cuisine have been known to possess major anti-inflammatory effects. Turmeric, red pepper, ginger, cinnamon and cumin  etc. are a few of the wide spectrum of spices used in Algeria. The aim of the study was to investigate the antiinflammatory effect  and anti actvity of some dietary spices in carrageenan induced models of inflammation on Wistar  rats. Methods: Albino wistar rats were divided into four groups. Inflammation was induced on the animal by injecting the right hand paw with carrageenan (0.1 ml of 1%). Group 1  was fed with food and water and  treated with carrageenan (control) whereas group 2 and  3 treated with different doses (100 and 200 mg/kg/bw) of aqueous extract of some dietary spices with carrageenan, respectively. Group 4  treated with standard drug diclofenac sodium (10 mg/kg b.w., reference drug for inflammation), once for 24 hrs. Results: The content of total phenolic was 137.2±21.6 mg/g and flavonoids 122.3±5.8 mg/g. After 60, 120, 180, 240 and 300 min, the data indicate that  some dietary spices 100 and 200 mg/kg was significantly effective reducing paw edema volumes induced by carrageenan compared to control (p <0.01). The levels of serum pro-inflammatory white blood cells, neutrophile, lymphocyte, monocyte,  eosinophile, total protein and protein c reactive and total cephalin KADIN  and platelts in the control group were significantly increased by carrageenan injection compared with the normal group. The rats pretreated with some spices 100 and 200 mg/kg had significantly lower levels of pro-inflammatory to compare control group. The paw edema in carrageenan-induced rats was considerably reduced by treating with 100 and 200  mg/kg aqueous extract of some dietary spices when compared to the untreated  rats (p<0.001). Conclusion : The present results clearly demonstarted that carrageenan significantly increased paw edema whereas some dietary treated rats significantly decreased the paw edema. The study underlines the importance of some of the frequently used spices (Turmeric, red pepper, ginger, cinnamon and cumin)  in the treatment of inflammation.

scholarly journals Anti-inflammatory potential of ethanol leaf extract of Sphenocentrum jollyanum in experimental mice

2021 ◽  
Vol 7 (1) ◽  
pp. 146-156
Author(s):  
Emmanuel Uka ◽  
Efosa Godwin Ewere ◽  
Grace Sylvester Effiong
Keyword(s):  
Leaf Extract ◽  
Folk Medicine ◽  
Control Group ◽  
Standard Drug ◽  
Egg Albumin ◽  
Paw Oedema ◽  
Anti Inflammatory ◽  
Dose Dependent ◽  
Ear Oedema

Anti-inflammatory potential of the ethanol leaf extract of Sphenocentrum jollyanum (ELESJ) was assessed in-vivo in mice by using carrageenan-induced paw oedema, egg albumin-induced paw oedema, and xylene-induced ear oedema model at doses of 474.34, 948.68, and 1423.03 mg/kgbw respectively. A total of seventy-five matured mice of both sexes weighing between 20 g and 24 g (divided into three groups of 25 mice each for the 3 different studies) were used for this study. Treatment was done intraperitoneally. Distilled water (10 mL/kgbw), acetylsalicylic acid (ASA) (100 mg/kgbw) or dexamethasone (4 mg/kgbw) served as the normal and standard drugs respectively. The results showed that ELESJ produced significant (p < 0.05), dose-dependent and time-dependent reductions in mean paw thickness in groups III-V compared with control group. Similarly, the extract caused a dose-dependent increase in percentage inhibition of carrageenan-induced paw oedema and egg-albumin induced paw oedema (34.49%, 36.71%) at the highest dose (1423.03 mg/kg), comparable with the anti-inflammatory effects of the standard drug ASA (34.69%, 31.54%). The ELESJ also caused significant (p < 0.05), dose-dependent decreases in xylene-induced ear oedema weight in all the test groups relative to control group and standard drug group. In addition, ELESJ showed a dose-dependent percentage inhibitory effect (44%) at the highest dose (1423.03 mg/kg) comparable with that of the standard drug, dexamethasone (48%). The results obtained from this study support the use of the leaf in folk medicine as it has significant anti-inflammatory properties and this may be attributed to the phytochemical constituents of the leaf.

scholarly journals In vivo anti-psoriasis, anti-inflammatory activities and oral toxicity studies of aqueous extract from seeds of Ammi visnaga L.

2020 ◽  
Vol 11 (SPL4) ◽  
pp. 1025-1037
Author(s):  
Ez-zahir Abdeljaouad ◽  
Naya Abdallah ◽  
Seddik Nadia ◽  
Marnissi Farida ◽  
Belghmi Khalid ◽  
...  
Keyword(s):  
Acute Toxicity ◽  
Aqueous Extract ◽  
Control Group ◽  
Oral Toxicity ◽  
Distribution Width ◽  
Toxicity Studies ◽  
Ammi Visnaga ◽  
Significant Difference ◽  
Anti Inflammatory

Ammi visnaga L (A.V.) is used in the traditional medicine for the treatment of kidney stones, diabetes and vitiligo. An in vivo anti-psoriasis, anti-inflammatory activities and oral toxicity studies of aqueous extract from seeds of Ammi visnaga L. was investigated. In the acute toxicity, the extract was administered orally in a single dose to rats (0- 2000-5000 mg/kg) and in the sub-acute toxicity daily for 28 days (0-300-600-1000 mg/kg/day). The symptoms of toxicity and mortality have been recorded daily and during 14 days of recovery with an examination of liver, kidney, hematologic, biochemical and histological analysis at the end of treatment. The anti-inflammatory activity was evaluated by induction of oedema and the anti-psoriasis by induction of a psoriasiform-like skin phenotype by UV-B radiations. No mortality was observed after single gavages by a dose up to 5000 mg/kg and no signs of toxicity noted. Clinical and biochemical examination during 28 days of gavages at all doses showed no significant difference compared to control group, while a significant reduction in MCV (mean corpuscular volume) and P-LCR (platelet large cell ratio) (p <0.05), PDW (platelet distribution width) and MPV (multi-purpose vehicle) (p <0.01) was observed and histopathological examinations showed slight inflammation in the liver and kidneys for the higher dose. Percentage of inhibition of the oedema was near the positive control 50% for all doses tested. Treatment with A.V. extract had decreased the thickness of the skin induced by UV-B irradiation.  In conclusion, the LD50 was estimated greater than 5000 mg/kg; therefore A.V. can be classified as non-toxic but if used in the long term can induce a slight toxicity dose dependant with high anti-psoriasis and anti-inflammatory activities.

N-arylmethylideneaminophthalimide: Design, Synthesis and Evaluation as Analgesic and Anti-inflammatory Agents

2019 ◽  
Vol 19 (8) ◽  
pp. 679-687 ◽  
Author(s):  
Nasimossadat Banarouei ◽  
Asghar Davood ◽  
Hamed Shafaroodi ◽  
Ghazaleh Saeedi ◽  
Abbas Shafiee
Keyword(s):  
Room Temperature ◽  
Antiinflammatory Activity ◽  
Chemical Characterization ◽  
Design Synthesis ◽  
Reference Drug ◽  
Anti Inflammatory ◽  
Pain Test ◽  
Derivatives Of

Background and Objective: N-aryl derivatives of phthalimide and 4-nitro phthalimide have demonstrated cyclooxygenase inhibitory activity. Also, they possess excellent analgesic and antiinflammatory activity. In this work, a new series of N-arylmethylideneamino derivatives of phthalimide and 4-nitro phthalimide were designed and synthesized. Methods: The designed compounds were synthesized by condensation of the appropriate aldehyde and N-aminophthalimide in ethanol at room temperature at PH around 3. Their analgesic and antiinflammatory activity were evaluated by acetic acid-induced pain test and carrageenan-induced paw edema test in mice and rats, respectively. Results and Conclusion:: The details of the synthesis and chemical characterization of the analogs are described. In vivo screening showed compounds 3a, 3b, 3f and 3h were the most potent analgesic compounds. In addition, compounds 3a, 3c, 3d, 3e and 3j indicated comparable anti-inflammatory activity to indomethacin as a reference drug.

scholarly journals TPGS-Stabilized Curcumin Nanoparticles Exhibit Superior Effect on Carrageenan-Induced Inflammation in Wistar Rat

2016 ◽  
Author(s):  
Heni Rachmawati ◽  
Dewi Safitri ◽  
Aditya Trias Pradana ◽  
I Ketut Adnyana
Keyword(s):  
Oral Absorption ◽  
Wide Spectrum ◽  
Wistar Rat ◽  
Standard Drug ◽  
Curcumin Nanoparticles ◽  
Edema Model ◽  
Polyethylene Glycol 1000 ◽  
Anti Inflammatory ◽  
Inflammatory Effect

Curcumin, a hydrophobic polyphenol compound derived from the rhizome of the Curcuma genus, has a wide spectrum of biological and pharmacological applications. Previously, curcumin nanoparticles with different stabilizers had been produced successfully in order to enhance solubility and per oral absorption. In the present study, we tested the anti-inflammatory effect of D-&alpha;-Tocopheryl polyethylene glycol 1000 succinate (TPGS)-stabilized curcumin nanoparticles in vivo. Lambda-carrageenan (&lambda;-carrageenan) was used to induce inflammation in rats; it was given by an intraplantar route and intrapelurally through surgery in the pleurisy test. In the &lambda;-carrageenan-induced edema model, TPGS-stabilized curcumin nanoparticles were given orally one hour before induction and at 0.5, 4.5, and 8.5 h after induction with two different doses (1.8 and 0.9 mg/kg body weight (BW)). Sodium diclofenac with a dose of 4.5 mg/kg BW was used as a standard drug. A physical mixture of curcumin-TPGS was also used as a comparison with a higher dose of 60 mg/kg BW. The anti-inflammatory effect was assessed on the edema in the carrageenan-induced paw edema model and by the volume of exudate as well as the number of leukocytes reduced in the pleurisy test. TPGS-stabilized curcumin nanoparticles with lower doses showed better anti-inflammatory effects, indicating the greater absorption capability through the gastrointestinal tract.

scholarly journals TPGS-Stabilized Curcumin Nanoparticles Exhibit Superior Effect on Carrageenan-Induced Inflammation in Wistar Rat

2016 ◽  
Author(s):  
Heni Rachmawati ◽  
Dewi Safitri ◽  
Aditya Trias Pradana ◽  
I Ketut Adnyana
Keyword(s):  
Oral Absorption ◽  
Wide Spectrum ◽  
Wistar Rat ◽  
Standard Drug ◽  
Curcumin Nanoparticles ◽  
Edema Model ◽  
Superior Effect ◽  
Anti Inflammatory ◽  
Inflammatory Effect

Curcumin, a hydrophobic polyphenol compound derived from the rhizome of Curcuma genus has wide spectrum of biological and pharmacological activities. Previously, curcumin nanocrystal with different stabilizers had been produced successfully in order to enhance solubility and per oral absorption. In present study, we tested the anti-inflammatory effect of TPGS-stabilized curcumin nanocrystal in vivo. Lambda-carrageenan was used to induce inflammation in rat, given by intraplantar route and intrapelurally through surgery in the pleurisy test. In lambda-carrageenan-induced edema model, TPGS-stabilized curcumin nanocrystal was given orally 1 hour before induction and at 0.5, 4.5, and 8.5 hour after induction with 2 different doses (1.8 and 0.9 mg/kg BW). Sodium diclofenac with a dose of 4.5 mg/kg BW was used as a standard drug. Physical mixture of curcumin-TPGS was also used as a comparison with higher dose of 60 mg/kg BW. The anti-inflammatory effect was assessed on the edema in carrageenan-induced paw edema model and volume of exudate as well as number of leukocytes reduction in the pleurisy test. TPGS-stabilized curcumin nanocrystal with lower doses showed better anti-inflammatory effect, indicating the greater absorption capability through gastrointestinal tract.

scholarly journals Anti-Inflammatory and Antinociceptive Activities of Untreated, Germinated, and Fermented Mung Bean Aqueous Extract

2014 ◽  
Vol 2014 ◽  
pp. 1-6 ◽  
Author(s):  
Norlaily Mohd Ali ◽  
Hamidah Mohd Yusof ◽  
Swee-Keong Yeap ◽  
Wan-Yong Ho ◽  
Boon-Kee Beh ◽  
...  
Keyword(s):  
Aqueous Extract ◽  
Mung Bean ◽  
Inflammatory Mediator ◽  
Dependent Manner ◽  
Antinociceptive Effects ◽  
Anti Inflammatory ◽  
Dose Dependent ◽  
Ear Oedema

Evaluation of anti-inflammatory and antinociceptive activities of untreated mung bean (MB), germinated mung bean (GMB), and fermented mung bean (FMB) was performed on bothin vitro(inhibition of inflammatory mediator, nitric oxide(NO)) andin vivo(inhibition of ear oedema and reduction of response to pain stimulus) studies. Results showed that both GMB and FMB aqueous extract exhibited potent anti-inflammatory and antinociceptive activities in a dose-dependent manner.In vitroresults showed that GMB and FMB were potent inflammatory mediator (NO) inhibitors at both 2.5 and 5 mg/mL. Furtherin vivostudies showed that GMB and FMB aqueous extract at 1000 mg/kg can significantly reduce ear oedema in mice caused by arachidonic acid. Besides, both 200 mg/kg and 1000 mg/kg concentrations of GMB and FMB were found to exhibit potent antinociceptive effects towards hotplate induced pain. With these, it can be concluded that GMB and FMB aqueous extract exhibited potential anti-inflammatory and antinociceptive effects.

scholarly journals Antioxidant Action and In Vivo Anti-Inflammatory and Antinociceptive Activities of Myrciaria floribunda Fruit Peels: Possible Involvement of Opioidergic System

2020 ◽  
Vol 2020 ◽  
pp. 1-11
Author(s):  
Izabelly Bianca da Silva Santos ◽  
Bruno Santos dos Santos ◽  
João Ricardhis Saturnino de Oliveira ◽  
Wêndeo Kennedy Costa ◽  
Adrielle Zagmignan ◽  
...  
Keyword(s):  
High Performance ◽  
Antioxidant Properties ◽  
Chemical Characterization ◽  
Opioidergic System ◽  
Anti Inflammatory ◽  
Inflammatory Action ◽  
Monomeric Anthocyanins

This work evaluated the antioxidant properties and in vivo antinociceptive and anti-inflammatory effects of extracts obtained from fruit peels of Myrciaria floribunda (H. West ex Willd.) O. Berg (Myrtaceae). This plant is popularly known in Brazil as Cambuí or camboim. Different extracts were submitted to comparative analysis to determine the content of selected phytochemical classes (levels of total phenols, flavonoids, and monomeric anthocyanins) and the in vitro antioxidant potentials. The extract with higher potential was selected for in vivo evaluation of its antinociceptive and anti-inflammatory action. Finally, the chemical characterization of this extract was performed by high-performance liquid chromatography (HPLC). MfAE (extract obtained using acetone as solvent) showed the higher levels of phenols (296 mg GAE/g) and anthocyanins contents (35.65 mg Cy-3-glcE/g) that were associated with higher antioxidant activity. MfAE also exhibited in vivo anti-inflammatory and analgesic propertiers. This fraction inhibited the inflammatory and neurogenic phases of pain, and this effect was reversed by naloxone (suggesting the involvement of opioidergic system). MfAE reduced the abdominal contortions induced by acetic acid. The HPLC analysis revealed the presence of gallic acid (and its derivatives) and ellagic acid. Taken together, these data support the use of M. floribunda fruit peels for development of functional foods and nutraceutics.

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