Relation between intestinal iNOS activation, epithelial cell viability and vascular leakage following Helicobacter pylori endotoxin challenge

Abstract Background: Zuojin Pill (ZJP), a famous Chinese medicinal formula, widely accepted for treatment of chronic atrophic gastritis (CAG) in China. This study aimed to explore the therapeutic effects and mechanisms of ZJP in Helicobacter pylori (H. pylori) - induced chronic atrophic gastritis (CAG) in vivo and in vitro. Methods: CAG rat model was induced by H. pylori. ZJP (0.63, 1.26, and 2.52 g/kg, respectively) was administered orally for four weeks. Therapeutic effects of ZJP were identified by H&E staining and serum indices. In addition, cell viability, morphology and proliferation were detected by cell counting kit-8 (CCK8) and high-content screening assay (HCS), respectively. Moreover, relative mRNA expression and protein expression related to JMJD2B/COX-2/VEGF axis was detected to investigate the potential mechanisms of ZJP in CAG. Results: Results showed the symptoms (weight loss and gastric mucosa damage) of CAG were alleviated, and the contents of TNF-α in serum was markedly decreased after treating with ZJP. Moreover, cell viability, proliferation and morphology changes of GES-1 cells were ameliorated by ZJP intervention. In addition, proinflammatory genes and JMJD2B/COX-2/VEGF axis related genes were suppressed by ZJP administration in vitro and in vivo. Meanwhile, immunohistochemistry (IHC) and western blot confirmed down-regulation of these genes by ZJP intervention. Conclusion: ZJP treatment can alleviate gastric mucosal damage induced by H. pylori via JMJD2B/COX-2/VEGF axis.

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CELL VIABILITY IN X - RAY IRRADIATED HUMAN LENS EPITHELIAL CELL (HLEC) CULTURE BY USING TRYPAN BLUE EXCLUSION TEST

Journal of Evidence Based Medicine and Healthcare ◽

10.18410/jebmh/2015/1277 ◽

2015 ◽

Vol 2 (60) ◽

pp. 9003-9008

Author(s):

Rajesh Dehankar ◽

Shivani Bapat ◽

Ksheersagar D D ◽

Paikrao V

Keyword(s):

Epithelial Cell ◽

Cell Viability ◽

Trypan Blue ◽

Lens Epithelial Cell ◽

Human Lens ◽

Trypan Blue Exclusion ◽

X Ray ◽

Human Lens Epithelial Cell ◽

Trypan Blue Exclusion Test

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Comparative In Vitro Sensitivities of Human Immune Cell Lines, Vaginal and Cervical Epithelial Cell Lines, and Primary Cells to Candidate Microbicides Nonoxynol 9, C31G, and Sodium Dodecyl Sulfate

Antimicrobial Agents and Chemotherapy ◽

10.1128/aac.46.7.2292-2298.2002 ◽

2002 ◽

Vol 46 (7) ◽

pp. 2292-2298 ◽

Cited By ~ 46

Author(s):

Fred C. Krebs ◽

Shendra R. Miller ◽

Bradley J. Catalone ◽

Raina Fichorova ◽

Deborah Anderson ◽

...

Keyword(s):

Epithelial Cell ◽

Sodium Dodecyl Sulfate ◽

Cell Viability ◽

Cell Lines ◽

Sodium Dodecyl ◽

Primary Cells ◽

Cytokine Activation ◽

Dodecyl Sulfate ◽

Human Immune

ABSTRACT In experiments to assess the in vitro impact of the candidate microbicides nonoxynol 9 (N-9), C31G, and sodium dodecyl sulfate (SDS) on human immune and epithelial cell viability, cell lines and primary cell populations of lymphocytic and monocytic origin were generally shown to be equally sensitive to exposures ranging from 10 min to 48 h. However, U-937 cells were more sensitive to N-9 and C31G after 48 h than were primary monocyte-derived macrophages. Cytokine activation of monocytes and lymphocytes had no effect on cell viability following exposure to these microbicidal compounds. Primary and passaged vaginal epithelial cultures and cell lines differed in sensitivity to N-9 and C31G but not SDS. These studies provide a foundation for in vitro experiments in which cell lines of human immune and epithelial origin can be used as suitable surrogates for primary cells to further investigate the effects of microbicides on cell metabolism, membrane composition, and integrity and the effects of cell type, proliferation, and differentiation on microbicide sensitivity.

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