Application of a charge-coupled device photon-counting technique to three-dimensional element analysis of a plant seed (alfalfa) using a full-field x-ray fluorescence imaging microscope

2007 ◽  
Vol 78 (7) ◽  
pp. 073706 ◽  
Author(s):  
Masato Hoshino ◽  
Toyoaki Ishino ◽  
Takashi Namiki ◽  
Norimitsu Yamada ◽  
Norio Watanabe ◽  
...  
Keyword(s):  
Fluorescence Imaging ◽  
Photon Counting ◽  
Three Dimensional ◽  
Element Analysis ◽  
Plant Seed ◽  
Charge Coupled Device ◽  
Full Field ◽  
X Ray ◽  
Dimensional Element ◽  
A Charge

Three-dimensional X-ray fluorescence imaging modes for biological specimens using a full-field energy dispersive CCD camera

2019 ◽  
Vol 34 (10) ◽  
pp. 2083-2093 ◽  
Author(s):  
Björn De Samber ◽  
Oliver Scharf ◽  
Günther Buzanich ◽  
Jan Garrevoet ◽  
Pieter Tack ◽  
...  
Keyword(s):  
Fluorescence Imaging ◽  
Biological Samples ◽  
Three Dimensional ◽  
Ccd Camera ◽  
Energy Dispersive ◽  
Field Energy ◽  
Full Field ◽  
X Ray ◽  
Pros And Cons

New three-dimensional full-field XRF imaging schemes are demonstrated using biological samples: the pros and cons with respect to traditional scanning XRF techniques are given.

Operation of a single-photon–counting x-ray charge-coupled device camera spectrometer in a petawatt environment

2004 ◽  
Vol 75 (10) ◽  
pp. 3705-3707 ◽  
Author(s):  
C. Stoeckl ◽  
W. Theobald ◽  
T. C. Sangster ◽  
M. H. Key ◽  
P. Patel ◽  
...  
Keyword(s):  
Single Photon ◽  
Photon Counting ◽  
Charge Coupled Device ◽  
Single Photon Counting ◽  
X Ray

Calibration and Monte Carlo simulation of a single-photon counting charge-coupled device for single-shot X-ray spectrum measurements

2013 ◽  
Vol 11 (11) ◽  
pp. 110401-110404 ◽  
Author(s):  
Yonghong Yan Yonghong Yan ◽  
Lai Wei Lai Wei ◽  
Xianlun Wen Xianlun Wen ◽  
Yuchi Wu Yuchi Wu ◽  
Zongqing Zhao Zongqing Zhao ◽  
...  
Keyword(s):  
Monte Carlo Simulation ◽  
Monte Carlo ◽  
Single Photon ◽  
Photon Counting ◽  
Single Shot ◽  
Charge Coupled Device ◽  
Single Photon Counting ◽  
X Ray ◽  
Spectrum Measurements

Defining the Tools: an Analysis of Laser Scanning Confocal and Wide-Field/Restoration Fluorescence Microscope Imaging

2001 ◽  
Vol 7 (S2) ◽  
pp. 1002-1003
Author(s):  
Jason R. Swedlow ◽  
Paul D. Andrews ◽  
Ke Hu ◽  
David S. RoosT ◽  
John M. Murray
Keyword(s):  
Fluorescence Imaging ◽  
Laser Scanning ◽  
Image Point ◽  
Fluorescence Signal ◽  
Wide Field ◽  
Charge Coupled Device ◽  
Standard Tool ◽  
Microscope Imaging ◽  
A Charge ◽  
Cellular Components

Digital fluorescence microscopy is now a standard tool for determining the localization of cellular components in fixed and living cells. Two fundamentally different imaging technologies are available for imaging fluorescently labelled cells and tissues, in either the fixed or living state. The laser scanning microscope uses a diffraction-limited focused beam to scan the sample and develop an image point by point. in addition, a pinhole placed in a plane confocal to the specimen prevents emitted out-of focus fluorescence from reaching the photomultiplier tube (PMT) detector. By combining spot illumination and selection of infocus fluorescence signal, the laser scanning confocal microscope (LSCM) creates an image of the specimen largely free of out-of-focus blur. By contrast, a wide-field microscope (WFM) illuminates the whole specimen simultaneously and detects the signal with a spatial array of point detectors, usually a charge-coupled device camera (CCD). This approach collects an image of all points of the specimen simultaneously and includes all the out-of-focus blurred light. Subsequent restoration by iterative deconvolution generates an estimate of the specimen, largely free of out-of-focus blur. While many other fluorescence imaging modalities exist, these two methods represent the majority of the fluorescence imaging systems currently in use in biomedical research.

scholarly journals Three-dimensional imaging of chemical phase transformations at the nanoscale with full-field transmission X-ray microscopy

2011 ◽  
Vol 18 (5) ◽  
pp. 773-781 ◽  
Author(s):  
Florian Meirer ◽  
Jordi Cabana ◽  
Yijin Liu ◽  
Apurva Mehta ◽  
Joy C. Andrews ◽  
...  
Keyword(s):  
Phase Transformations ◽  
Three Dimensional ◽  
Three Dimensional Imaging ◽  
Full Field ◽  
X Ray ◽  
Chemical Phase

Full-field X-ray fluorescence imaging with a straight polycapillary X-ray collimator

2020 ◽  
Vol 15 (12) ◽  
pp. P12033-P12033
Author(s):  
S. An ◽  
D. Krapohl ◽  
B. Norlin ◽  
G. Thungström
Keyword(s):  
Fluorescence Imaging ◽  
Full Field ◽  
X Ray

Nanoscale Three-Dimensional Microstructural Characterization of an Sn-Rich Solder Alloy Using High-Resolution Transmission X-Ray Microscopy (TXM)

2016 ◽  
Vol 22 (4) ◽  
pp. 808-813 ◽  
Author(s):  
Chandrashekara S. Kaira ◽  
Carl R. Mayer ◽  
V. De Andrade ◽  
Francesco De Carlo ◽  
Nikhilesh Chawla
Keyword(s):  
Fresnel Zone ◽  
Three Dimensional ◽  
Microstructural Characterization ◽  
Second Phase ◽  
Full Field ◽  
X Ray ◽  
Second Phase Particles ◽  
Rich Alloy ◽  
Nanoscale Features

AbstractThree-dimensional (3D) nondestructive microstructural characterization was performed using full-field transmission X-ray microscopy on an Sn-rich alloy, at a spatial resolution of 60 nm. This study highlights the use of synchrotron radiation along with Fresnel zone plate optics to perform absorption contrast tomography for analyzing nanoscale features of fine second phase particles distributed in the tin matrix, which are representative of the bulk microstructure. The 3D reconstruction was also used to quantify microstructural details of the analyzed volume.

scholarly journals Measurement of three-dimensional displacement field in piled embankments using synchrotron X-ray tomography

2019 ◽  
Vol 56 (6) ◽  
pp. 885-892 ◽  
Author(s):  
Louis King ◽  
Abdelmalek Bouazza ◽  
Anton Maksimenko ◽  
Will P. Gates ◽  
Stephen Dubsky
Keyword(s):  
High Resolution ◽  
Imaging Techniques ◽  
Three Dimensional ◽  
Physical Models ◽  
Scale Model ◽  
Small Scale ◽  
Full Field ◽  
X Ray ◽  
Wide Range ◽  
Piled Embankments

The measurement of displacement fields by nondestructive imaging techniques opens up the potential to study the pre-failure mechanisms of a wide range of geotechnical problems within physical models. With the advancement of imaging technologies, it has become possible to achieve high-resolution three-dimensional computed tomography volumes of relatively large samples, which may have previously resulted in excessively long scan times or significant imaging artefacts. Imaging of small-scale model piled embankments (142 mm diameter) comprising sand was undertaken using the imaging and medical beamline at the Australian Synchrotron. The monochromatic X-ray beam produced high-resolution reconstructed volumes with a fine texture due to the size and mineralogy of the sand grains as well as the phase contrast enhancement achieved by the monochromatic X-ray beam. The reconstructed volumes were well suited to the application of digital volume correlation, which utilizes cross-correlation techniques to estimate three-dimensional full-field displacement vectors. The output provides insight into the strain localizations that develop within piled embankments and an example of how advanced imaging techniques can be utilized to study the kinematics of physical models.

scholarly journals Virtual paleontology: computer-aided analysis of fossil form and function

2014 ◽  
Vol 88 (4) ◽  
pp. 633-635 ◽  
Author(s):  
Imran A. Rahman ◽  
Selena Y. Smith
Keyword(s):  
Imaging Techniques ◽  
Three Dimensional ◽  
Fossil Plants ◽  
Element Analysis ◽  
X Ray ◽  
Form And Function ◽  
Wide Range ◽  
History Of ◽  
Micro Tomography ◽  
And Function

‘Virtual paleontology’ entails the use of computational methods to assist in the three-dimensional (3-D) visualization and analysis of fossils, and has emerged as a powerful approach for research on the history of life. Three-dimensional imaging techniques allow poorly understood or previously unknown anatomies of fossil plants, invertebrates, and vertebrates, as well as microfossils and trace fossils, to be described in much greater detail than formerly possible, and are applicable to a wide range of preservation types and specimen sizes (Table 1). These methods include non-destructive high-resolution scanning technologies such as conventional X-ray micro-tomography and synchrotron-based X-ray tomography. In addition, form and function can be rigorously investigated through quantitative analysis of computer models, for example finite-element analysis.

Sign in / Sign up

Export Citation Format

Share Document