Antioxidant activity of apple snail crude extracts

2019 ◽  
Author(s):  
Nurkhalida Mohammad Khalil ◽  
Syarul Nataqain Baharum ◽  
Mohd Shazrul Fazry Sa’ariwijaya ◽  
Nik Marzuki Sidik ◽  
Fareed Sairi
Keyword(s):  
Antioxidant Activity ◽  
Apple Snail ◽  
Crude Extracts

Assessment of antioxidant and antibacterial activities of crude extracts of verbena officinalis Linn root or Atuch (Amharic)

2019 ◽  
Author(s):  
Chem Int
Keyword(s):  
Antioxidant Activity ◽  
Methanolic Extract ◽  
Radical Scavenging Activity ◽  
Radical Scavenging ◽  
Dry Weight ◽  
Antimicrobial Activities ◽  
Total Flavonoid ◽  
Root Extract ◽  
Antioxidant Power ◽  
Crude Extracts

Verbena officinalis Linn is a traditionally known medicinal plant which is used against a number of diseases including inflammatory conditions. In this study its antioxidant activity (reducing powers, 2, 2-diphenyl-1-picrylhydrazyl (DPPH) scavenging activities), ferric reduction activity potential (FRAP), total flavonoid concentration and antimicrobial activities of 80%, 90%, 100% methanol and chloroform extracts of V. officinalis Linn root and 90% and100% methanol leaf extracts were determined. Its antioxidant activity increases with increase in amount of extract (10% to 40%v/v). Total flavonoid content (TFC) varied from 73.32±0.002 mgQE/100g of dry weight (90% methanol) to 42.39±0.032 mgQE/100g dry weight (chloroform), 2,2-diphenyl-1-picrylhydrazyl (DPPH), radical scavenging activity (%) was varied between 87.39% (90% methanol) to 45.57% (chloroform) while Ferric reducing antioxidant power was observed between 372.93±0.04 mgAAE/100 g extract (90% methanol) to 129.41±0.026 mgAAE/100 g chloroform in the root extract. The methanolic extract of the leaf showed less antioxidant activity than the methanolic extract of the root. Crude extracts of V. officinalis root showed various degree of antimicrobial activity towards drug resistance microbial pathogens. Growth inhibition tests against bacterial pathogens demonstrated concentration dependence. Moreover, gram positive bacteria were more susceptible to V. officinalis root extract when compared to gram negative bacteria. In general V. officinalis root and leave extracts possess strong antioxidant and antimicrobial activities.

Antioxidant activity of crude extracts obtained from endophytic fungi isolated from Otoba gracilipes of dry tropical forest in Colombia

2018 ◽  
Vol 44 ◽  
pp. S77
Author(s):  
N.H. Caicedo ◽  
L.I. Cedeno ◽  
D.A. Jaramillo ◽  
P. Puente ◽  
M. Henao ◽  
...  
Keyword(s):  
Antioxidant Activity ◽  
Tropical Forest ◽  
Endophytic Fungi ◽  
Dry Tropical Forest ◽  
Crude Extracts

scholarly journals Production of eugenol from fungal endophytesNeopestalotiopsissp. andDiaporthesp. isolated fromCinnamomum loureiroileaves

PeerJ ◽  
2019 ◽  
Vol 7 ◽  
pp. e6427 ◽  
Author(s):  
Chutima Tanapichatsakul ◽  
Sarunpron Khruengsai ◽  
Sakon Monggoot ◽  
Patcharee Pripdeevech
Keyword(s):  
Antimicrobial Activity ◽  
Antioxidant Activity ◽  
Endophytic Fungi ◽  
Antioxidant Activities ◽  
Chemical Constituents ◽  
Fungal Endophytes ◽  
Biologically Active ◽  
Crude Extracts ◽  
Antioxidant Agents ◽  
Fungal Extracts

Endophytic fungi, which colonize within a host plant without causing any apparent diseases, have been considered as an important source of bioactive secondary metabolites containing antimicrobial and antioxidant activities. The aim of this research was to isolate the endophytic fungi ofCinnamomum loureiroiand then to screen their antimicrobial and antioxidant activities. A total of 11 fungal endophytes were isolated from healthy leaves ofCinnamomum loureiroibelonging to six genera:Botryosphaeria,Colletotrichum,Diaporthe,Fusarium,Neopestalotiopsis, andPestalotiopsis. All isolated strains were cultured and further extracted with ethyl acetate solvent. Antimicrobial activity of all crude endophytic fungal extracts was analyzed using disc diffusion assay against six bacterial and two fungal pathogens. Crude extracts of strains MFLUCC15-1130 and MFLUCC15-1131 showed broad-spectrum antimicrobial activity against all tested pathogens. Activity againstBacillus cereusandStaphylococcus epidermidiswas notable, showing the lowest minimum inhibitory concentration at 3.91 μg/mL. Antioxidant activity of all crude endophytic fungal extracts was also evaluated based on 2,2-diphenyl-1-picrylhydrazyl assay. Significant antioxidant activity was detected in the crude extracts of fungus MFLUCC15-1130 and MFLUCC15-1131 with IC50of 22.92 ± 0.67 and 37.61 ± 0.49 μg/mL, respectively. Using molecular identification, MFLUCC15-1130 and MFLUCC15-1131 were identified asNeopestalotiopsissp. andDiaporthesp., respectively. The major chemical constituents produced by both crude extracts were identified by gas chromatography-mass spectrometry. Eugenol, myristaldehyde, lauric acid, and caprylic acid were the primary antimicrobial and antioxidant compounds in both crude extracts. This is the first report of eugenol being a biologically active compound ofNeopestalotiopsissp. andDiaporthesp. fungal endophytes. Eugenol has been reported as antimicrobial and antioxidant agents with agronomic applications. Thus the two newly-isolated endophytes may be used for eugenol production, which in turn can be used in a variety of applications.

scholarly journals Antioxidative Activities and Active Compounds of Extracts fromCatalpaPlant Leaves

2014 ◽  
Vol 2014 ◽  
pp. 1-7 ◽  
Author(s):  
Hongyu Xu ◽  
Gege Hu ◽  
Juane Dong ◽  
Qin Wei ◽  
Hongbo Shao ◽  
...  
Keyword(s):  
Antioxidant Activity ◽  
Column Chromatography ◽  
Antioxidant Activities ◽  
Total Flavonoid ◽  
Total Flavonoid Content ◽  
Flavonoid Content ◽  
Crude Extracts ◽  
Catalpa Ovata ◽  
High Antioxidant Activity ◽  
Water Fractions

In order to screen theCatalpaplant with high antioxidant activity and confirm the corresponding active fractions fromCatalpa ovataG. Don,C. fargesiiBur., andC. bungeiC. A. Mey., total flavonoid contents and antioxidant activities of the extracts/fractions ofCatalpaplant leaves were determined. The determined total flavonoid content and antioxidant activity were used as assessment criteria. Those compounds with antioxidant activity were isolated with silica gel column chromatography and ODS column chromatography. Our results showed that the total flavonoid content inC. bungeiC. A. Mey. (30.07 mg/g·DW) was the highest, followed by those inC. fargesiiBur. (25.55 mg/g·DW) andC. ovataG. Don (24.96 mg/g·DW). According to the determination results of total flavonoid content and antioxidant activity in 3 clones of leaves ofC. bungeiC. A. Mey., the total flavonoid content and antioxidant activity in crude extracts fromC. bungeiC. A. Mey. 6 (CA6) leaves were the highest. Moreover, the results showed that the total flavonoid content and antioxidant activities of ethyl acetate (EA) fraction in ethanol crude extracts in CA6 leaves were the highest, followed byn-butanol, petroleum ether (PE), and water fractions. Two flavonoid compounds with antioxidant activity were firstly isolated based on EA fraction. The two compounds were luteolin (1) and apigenin (2), respectively.

scholarly journals Phytochemical analysis and antioxidant activity of different plant parts of Pellacalyx axillaris

2019 ◽  
Vol 15 (3) ◽  
pp. 394-397
Author(s):  
Najwa Ahmad Kuthi ◽  
Norazah Basar
Keyword(s):  
Antioxidant Activity ◽  
Biological Activities ◽  
Radical Scavenging ◽  
Bark Extract ◽  
Phytochemical Analysis ◽  
Phytochemical Study ◽  
Antioxidant Power ◽  
Plant Parts ◽  
Crude Extracts ◽  
Radical Scavenging Assay

Pellacalyx axillaris or locally known as ‘membuloh’ is a mangrove species belonging to the Rhizophoraceae family. Till date, there has been only one phytochemical study found on this particular plant species and that without any documentation on its biological activities. Therefore, the present work aimed to reveal the phytoconstituents and the antioxidant activity of different crude extracts from different plant parts of P. axillaris. Experimentally, three organic solvents of different polarities i.e. n-hexane, ethyl acetate and methanol were used to prepare the crude extracts from the dried leaves, twigs and barks of P. axillaris. The preliminary phytochemical screening of this species indicated the presence of terpenoids, phenolic compounds, tannins, flavonoids, alkaloids, anthraquinone glycosides and carbohydrates. The in vitro antioxidant activity of the species evaluated by 2,2-diphenyl-1-picrylhydrazyl (DPPH) radical scavenging assay, 2,2’-azino-bis(3-ethylbenzothiazoline-6-sulphonic acid) (ABTS) radical scavenging assay, and ferric reducing antioxidant power (FRAP) suggested that the methanolic bark extract contained potential source of natural antioxidants. Further research into isolation of antioxidant compounds from this species is highly recommended.

COMPARISON STUDY OF ANTIOXIDANT, ANTIMICROBIAL AND CYTOTOXIC PROPERTIES OF SECONDARY METABOLITE AND PROTEIN EXTRACTS FROM CLINACANTHUS NUTANS

2020 ◽  
pp. 98-102
Author(s):  
ASVINIDEVI ARUMUGAM ◽  
NYET KUI WONG
Keyword(s):  
Antioxidant Activity ◽  
Methanol Extract ◽  
Cytotoxic Agents ◽  
Diffusion Method ◽  
Crude Extracts ◽  
Brine Shrimp Lethality Assay ◽  
Ic50 Value ◽  
Brine Shrimps ◽  
Clinacanthus Nutans ◽  
Cytotoxicity Activities

Objective: The present study aims to evaluate the antioxidant, antimicrobial and cytotoxic properties of the secondary metabolites and proteins extracted from Clinacanthus nutans. Methods: Methanol (ME) and acetone (AC) and also protein (PE) extracts were obtained from Clinacanthus nutans. Antioxidant activity of sample extracts was examined by using 1,1-Diphenyl-2-Picrydyl Hydrazyl (DPPH) assay and antimicrobial activity was examined by using the agar diffusion method against Bacillus subtilis, Bacillus cereus, Streptococcus pyrogenes, Escherichia coli, and Pseudomonas aeroginosa bacterial strain. The cytotoxic potential was determined by brine shrimp lethality assay. Results: Methanol extract (ME) demonstrated to have the highest antioxidant activity with IC50 value of 98.84 μg/ml, followed by acetone extract (AE) with IC50 value of 134.83µg/ml, and protein extract (PE) with IC50 value of 353.49 µg/ml. Among all of the sample extracts, only methanol crude extracts (ME) displayed moderate inhibition against Gram-positive B. cereus (7.33±1.15 mm) and S. pyrogenes (8.67±0.57 mm) at concentration of 100 mg/ml, while both acetone (AE) and protein (PE) extracts had no activity against all tested microorganisms. All extracts from methanol (ME), acetone (AE) and protein (PE) exhibited significant cytotoxic activity against brine shrimps at LC50 7.2 μg/ml, 1.42 μg/ml and 70.6 μg/ml respectively. Conclusion: These data proved that methanol extract from the leaves of Clinacanthus nutans is the most potent among all samples tested and has the potential to be developed as an antioxidant, antimicrobial and cytotoxic agents. Whilst, acetone and protein crude extracts have potent antioxidant and cytotoxicity activities.

TOTAL PHENOLS CONTENT AND ANTIOXIDANT ACTIVITY OF DIFFERENT POLARITY CRUDE EXTRACTS OF DODONAEA VISCOSA

INDIAN DRUGS ◽  
2021 ◽  
Vol 58 (08) ◽  
pp. 79-83
Author(s):  
Maisa R. Shoriqi ◽  
Salem S. Touby ◽  
Mohammad A. Hossain ◽  
Keyword(s):  
Antioxidant Activity ◽  
Antioxidant Properties ◽  
Water Extract ◽  
Total Phenols ◽  
Dodonaea Viscosa ◽  
Leaf Extracts ◽  
Coarse Powder ◽  
Dry Extract ◽  
Crude Extracts ◽  
Antioxidant Agents

Nowadays, effective medications as antioxidant agents are mandatory for a safe and sustainable environment. Dodonaea viscosa (D. viscosa) is used traditionally by the Omani people to treat rheumatism, toothaches, fever, cold, malaria, headaches, indigestion, ulcers, diarrhea, and constipation, dysmenorrheal and irregular menstruation. This study was carried out to prepare leaf extracts by different solvents and to determine their antioxidant activity and total phenols content. The selected plant was collected locally near the University Campus, Nizwa, Oman. The dried coarse powder was used for the extraction with methanol and it was defatted with water and successively partitioned with different polarity solvents with increasing solvent polarity. The total phenols content and antioxidant activity of the prepared different extracts were assessed by Folin-Ciocalteu reagent (FCR) and 2,2-diphenyl-1-picryl-hydrazyl (DPPH) methods. The total phenols content of different extracts was in the range of 3.02-249.93 mg gallic acid/g dry extract. The chloroform extract showed the maximum amount of total phenol compounds (249.93 mg GAE acid/g dry extract) and the minimum content was found in water extract (3.02 mg GAE acid/g dry extract). The leaf crude extracts were obtained to significant levels of antioxidant activity that ranged from percentage of inhibition from 33-85.92 %. The water extract and n-butanol extracts showed significant levels of antioxidant activity (85.92 % and 84.99 %) against the DPPH free radical method. In conclusion, this study showed that different polarities crude extracts of D. viscosa comprise a significant amount of phenols plus antioxidant properties and have possibilities of being potential use of the selected species for a natural source of antioxidants.

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