TOTAL PHENOLS CONTENT AND ANTIOXIDANT ACTIVITY OF DIFFERENT POLARITY CRUDE EXTRACTS OF DODONAEA VISCOSA

INDIAN DRUGS ◽  
2021 ◽  
Vol 58 (08) ◽  
pp. 79-83
Author(s):  
Maisa R. Shoriqi ◽  
Salem S. Touby ◽  
Mohammad A. Hossain ◽  
Keyword(s):  
Antioxidant Activity ◽  
Antioxidant Properties ◽  
Water Extract ◽  
Total Phenols ◽  
Dodonaea Viscosa ◽  
Leaf Extracts ◽  
Coarse Powder ◽  
Dry Extract ◽  
Crude Extracts ◽  
Antioxidant Agents

Nowadays, effective medications as antioxidant agents are mandatory for a safe and sustainable environment. Dodonaea viscosa (D. viscosa) is used traditionally by the Omani people to treat rheumatism, toothaches, fever, cold, malaria, headaches, indigestion, ulcers, diarrhea, and constipation, dysmenorrheal and irregular menstruation. This study was carried out to prepare leaf extracts by different solvents and to determine their antioxidant activity and total phenols content. The selected plant was collected locally near the University Campus, Nizwa, Oman. The dried coarse powder was used for the extraction with methanol and it was defatted with water and successively partitioned with different polarity solvents with increasing solvent polarity. The total phenols content and antioxidant activity of the prepared different extracts were assessed by Folin-Ciocalteu reagent (FCR) and 2,2-diphenyl-1-picryl-hydrazyl (DPPH) methods. The total phenols content of different extracts was in the range of 3.02-249.93 mg gallic acid/g dry extract. The chloroform extract showed the maximum amount of total phenol compounds (249.93 mg GAE acid/g dry extract) and the minimum content was found in water extract (3.02 mg GAE acid/g dry extract). The leaf crude extracts were obtained to significant levels of antioxidant activity that ranged from percentage of inhibition from 33-85.92 %. The water extract and n-butanol extracts showed significant levels of antioxidant activity (85.92 % and 84.99 %) against the DPPH free radical method. In conclusion, this study showed that different polarities crude extracts of D. viscosa comprise a significant amount of phenols plus antioxidant properties and have possibilities of being potential use of the selected species for a natural source of antioxidants.

scholarly journals Phytochemicals and Quality of Green and Black Teas from Different Clones of Tea Plant

2020 ◽  
Vol 2020 ◽  
pp. 1-13
Author(s):  
Yadav KC ◽  
Ashok Parajuli ◽  
Bishnu Bahadur Khatri ◽  
Lila Devi Shiwakoti
Keyword(s):  
Antioxidant Activity ◽  
Green Tea ◽  
Antioxidant Properties ◽  
Water Extract ◽  
Black Tea ◽  
Tea Plant ◽  
Total Quality ◽  
Dry Extract ◽  
Caffeine Content ◽  
Tea Plants

Tea is a popular drink with refreshing and functional properties. Bud, 1st leaf, and 2nd leaf of five varieties of tea clones (Gumti Takda-78, Ambari, Chiniya, and Tinali, which are popular in tea plantation area of Nepal) were collected and used for preparing green and orthodox black tea to study antioxidant activity, phytochemicals profile, chemical content, and sensory parameters. One or two leaves were hand-plucked from each bush to get a sample of about 100 leaves and processed for green and orthodox black tea for different clones of tea plants. Phytochemicals, antioxidant activity, and caffeine content were found higher in bud followed by 1st leaf and 2nd leaf for all clones of tea plants. Both types of tea (green and black) from Gumti were significantly (p<0.05) higher having tannin content, flavonoid content, total polyphenol content, caffeine content, and IC50 value of 49.15 and 36.23 (mg GAE/g dry extract), 358.9 and 350.4 (mg QE/g dry extract), 590.5 and 570 (mg GAE/g dry extract), 2.85 and 2.94%, and 45.15 & 51.88 μg/mL, respectively. Green and orthodox tea from Takda-78 was found higher in caffeine content and the least in Tinali for both types of tea. Moisture, water extract, total ash, acid-insoluble ash, and crude fiber content in tea (green and black) from Gumti were found to be 5.4% & 5.37%, 65.89% & 71.46%, 5.524% & 6.52%, 0.46% & 0.57%, and 7.96% & 10.27%, respectively. The ratio of theaflavin and thearubigin (TF : TR) was found 1 : 8.61, 1 : 9.36, 1 : 9.70, 1 : 12.87, and 1 : 6.36 in Takda-78, Ambari, Gumti, Chiniya, and Tinali respectively. The total quality score in green tea (85.13%) and black tea (85.78%) from Gumti was significantly higher than others. Phytochemicals and antioxidant properties of green tea were significantly (p<0.05) higher than those of orthodox black tea for all clones of tea plant. This study suggests Gumti variety to be used in green and orthodox black tea processing for higher phytochemical, chemical, sensory quality, and antioxidant activity.

scholarly journals Total Phenolic Contents and Antioxidant Properties of Algerian Arbutus unedo L. Extracts

2020 ◽  
Vol 10 (3-s) ◽  
pp. 159-168
Author(s):  
Saliha Laouicha ◽  
Abderrahmane Senator ◽  
Abdallah Kherbache ◽  
Hamama Bouriche
Keyword(s):  
Antioxidant Activity ◽  
Folk Medicine ◽  
Antioxidant Properties ◽  
Total Phenolic ◽  
Phytochemical Analysis ◽  
Leaf Extracts ◽  
Arbutus Unedo ◽  
Phenolic Contents ◽  
Antioxidant Agents ◽  
Ic50 Values

Arbutus unedo L. is a plant widely distributed in the Mediterranean basin and North Africa, frequently used in traditional folk medicine to treat diabetes and arterial hypertension. This study aims to evaluate the phenolic composition and antioxidant activity of ethyl acetate (EA.E) and n-butanolic (But.E) extracts prepared from leaves of Arbutus unedo. Total phenolic and flavonoids contents were determined spectrophotometrically. The antioxidant activity was evaluated using DPPH•, OH•, H2O2, O•-2, ß-carotene bleaching and AAPH-induced erythrocyte oxidative hemolysis assays. The phytochemical analysis showed the presence of polyphenols and flavonoids in both extracts. The high amount was observed in EA.E which exerted the stronger antioxidant effect, with IC50 values of 3.43 μg/mL, 323.45 µg/mL, 38.40 µg/mL and 11.56 µg/mL, in DPPH•, OH•, O•-2 and H2O2 tests, respectively. Both extracts inhibited β-carotene bleaching, but EA.E is always more potent (92%) than But.E (85%). Furthermore, the EA.E showed the highest protective effect on erythrocyte hemolysis induced by AAPH, with half time hemolysis (HT50) of 122.02 min at 40 µg/mL. Taken together, this study showed that Arbutus unedo leaf extracts possess strong antioxidant potential, which may be attributed to the presence of a high amount of polyphenolic constituents. So, this plant might be exploited as a potential source of natural antioxidant agents for pharmaceutical and food applications. Keywords: oxidative stress, antioxidant, phenolic compounds, Arbutus unedo.

scholarly journals Synthesis and antioxidant properties of 2-(3-(hydroxyimino)methyl)-1H-indol-1-yl)acetamide derivatives

2020 ◽  
Vol 6 (1) ◽  
Author(s):  
Chandravadivelu Gopi ◽  
Magharla Dasaratha Dhanaraju
Keyword(s):  
Antioxidant Activity ◽  
Condensation Reaction ◽  
Antioxidant Properties ◽  
Side Chain ◽  
Antioxidant Power ◽  
H Nmr ◽  
Antioxidant Agents ◽  
Phenyl Rings ◽  
Ferric Reducing Antioxidant Power ◽  
Ft Ir

Abstract Background The main aim of this work was to synthesise a novel N-(substituted phenyl)-2-(3-(hydroxyimino) methyl)-1H-indol-1-yl) acetamide derivatives and evaluate their antioxidant activity. These compounds were prepared by a condensation reaction between 1H-indole carbaldehyde oxime and 2-chloro acetamide derivatives. The newly synthesised compound structures were characterised by FT-IR, 1H-NMR, mass spectroscopy and elemental analysis. Furthermore, the above-mentioned compounds were screened for antioxidant activity by using ferric reducing antioxidant power (FRAP) and 1,1-diphenyl-2-picrylhydrazyl (DPPH) methods. Result The antioxidant activity result reveals that most of the compounds were exhibiting considerable activity in both methods and the values are very closer to the standards. Among the synthesised compounds, compound 3j, 3a and 3k were shown remarkable activity at low concentration. Conclusion Compounds 3j, 3a and 3k were shown highest activity among the prepared analogues due to the attachment of halogens connected at the appropriate place in the phenyl ring. Hence, these substituted phenyl rings considered as a perfect side chain for the indole nucleus for the development of the new antioxidant agents.

scholarly journals In Vitro Antioxidant Activity of Litsea martabanica Root Extract and Its Hepatoprotective Effect on Chlorpyrifos-Induced Toxicity in Rats

Molecules ◽  
2021 ◽  
Vol 26 (7) ◽  
pp. 1906
Author(s):  
Phraepakaporn Kunnaja ◽  
Sunee Chansakaow ◽  
Absorn Wittayapraparat ◽  
Pedcharada Yusuk ◽  
Seewaboon Sireeratawong
Keyword(s):  
Antioxidant Activity ◽  
Antioxidant Properties ◽  
Water Extract ◽  
Acetylcholinesterase Activity ◽  
Liver Cells ◽  
Scientific Data ◽  
Total Phenolic ◽  
Root Extract ◽  
Antioxidant Power ◽  
Detoxifying Agent

In Thailand, people in the highland communities whose occupational exposure to pesticides used the root of Litsea martabanica as a detoxifying agent. However, the scientific data to support the traditional use of this plant are insufficient. This study aimed to evaluate the antioxidant activity and anti-pesticide potential of L. martabanica root extract. Antioxidant properties were investigated by 2,2′-diphenyl-1-picrylhydrazyl (DPPH) assay, superoxide radicals scavenging assay, 2,2′-azino-bis(3-ethylbenzothiazoline-6-sulfonic acid) (ABTS) assay, ferric reducing antioxidant power (FRAP), and total phenolic content determination. In all assays, L. martabanica extracts and their fractions exhibited high antioxidant activities differently. The water extract is traditionally used as a detoxifying agent. Therefore, it was chosen for in vivo experiments. The rats received the extract in a way that mimics the traditional methods of tribal communities followed by chlorpyrifos for 16 days. The results showed that acetylcholinesterase activity decreases in pesticide-exposed rats. Treatment with the extract caused increasing acetylcholinesterase activity in the rats. Therefore, L. martabanica extract may potentially be used as a detoxifying agent, especially for the chlorpyrifos pesticide. The antioxidant properties of L. martabanica may provide a beneficial effect by protecting liver cells from damage caused by free radicals. Histopathology results revealed no liver cell necrosis and showed the regeneration of liver cells in the treatment group. L. martabanica extract did not cause changes in behavior, liver weight, hematological and biochemical profiles of the rats.

scholarly journals EFFECT OF DIFFERENT SOLVENTS ON ANTIOXIDANT ACTIVITY OF LEAF EXTRACTS OF CALOTROPIS PROCERA AND AZADIRACHTA INDICA

2016 ◽  
Vol 10 (1) ◽  
pp. 268
Author(s):  
Sharmistha Banerjee ◽  
Shuchi Kaushik ◽  
Rajesh Singh Tomar
Keyword(s):  
Antioxidant Activity ◽  
Total Phenolics ◽  
Radical Scavenging Activity ◽  
Radical Scavenging ◽  
Calotropis Procera ◽  
Peroxide Radical ◽  
Scavenging Activity ◽  
Leaf Extracts ◽  
Dry Extract ◽  
Leaf Powder

ABSTRACTObjective: This study was performed to identify the phytochemicals and comparatively evaluate the antioxidant activity of Calotropis procera andAzadirachta indica by detection of total phenolics, hydrogen peroxide radical scavenging activity, and estimation of condensed tannins in differentsolvent systems and at different temperatures.Methods: Leaves of C. procera and A. indica were extracted in water, methanol by soaking dried leaf powder at room temperature and also by boilingthe leaf powder in water for 30 minutes. Phytochemical tests were performed in all of the extracts. The antioxidant activity was determined byhydrogen peroxide radical scavenging activity. Quantitative estimation of total phenolics and hydrolysable tannins was also performed.Results: The total phenolics in both leaf extracts was obtained maximum in boiled extract (40.7±1.20 mg gallic acid equivalent [GAE]/g dry extractin C. procera and 33.66±1.45 mg GAE/g dry extract in A. indica). The amount of hydrolysable tannins in both leaf extracts was found to be highest inmethanol (150±1.88 mg catechin equivalent/g dry extract in C. procera and 144.8±2.63 mg catechin equivalent/g dry extract in A. indica).Conclusion: The study showed promising results indicating that these plants are a good source of antioxidants. The majority of phytochemicals wereextracted in distilled water and methanol acts as a good solvent for extraction of tannins, whereas an increase in temperature leads to poor extractionof tannins.Keywords: Antioxidant, Phytochemicals, Phenolics, Radical, Tannins.© 2017 The Authors. Published by Innovare Academic Sciences Pvt Ltd. This is an open access article under the CC BY license (http://creativecommons.

scholarly journals Antioxidant properties of hot water extracts from carpophore and spores of mushroom Ganoderma lucidum

2011 ◽  
pp. 279-288 ◽  
Author(s):  
Anita Klaus ◽  
Maja Kozarski ◽  
Miomir Niksic
Keyword(s):  
Antioxidant Activity ◽  
Ganoderma Lucidum ◽  
Antioxidant Properties ◽  
Water Extract ◽  
Reducing Power ◽  
Hot Water ◽  
Ferrous Ions ◽  
Medicinal Mushrooms ◽  
Water Extracts ◽  
Hot Water Extract

Ganoderma lucidum (Leyss.:Fr.) Karst is one of the medicinal mushrooms, which possesses enviable antioxidant properties. Objective of this investigation was to evaluate antioxidant activity, reducing power, scavenging abilities on 1.1-diphenyl-2-picrylhydrazyl (DPPH) radicals and chelating effects on ferrous ions of hot water extracts obtained from carpophore and spores of this mushroom. Hot water extract from carpophore (Gl-I) showed high antioxidant activity of 85.7 ? 0.7%, at 10 mg/ml, while antioxidant activity of hot water extract from spores (Gl-Is) was 9.2 ? 0.3% at 10 mg/ml. Reducing power of Gl-I reached a plateau of 3.4 ? 0.1 at 20 mg/ml, and 0.3 ? 0.0 at 20 mg/ml for Gl-Is. At 10 mg/ml, scavenging ability on DPPH radicals of Gl-I increased to 96.8 ? 2.5%, whereas Gl-Is scavenged DPPH radicals by 69.6 ? 2.5% at 10 mg/ml. Gl-I chelated 81.6 ? 3.6 % of ferrous ions at 20 mg/ml, while the chelating effect of Gl-Is was 73.8 ? 1.7%. The antioxidative activities of hot water extracts from carpophore and spores of the mushroom G. lucidum were concentration dependent and increased with an increase in the concentration.

scholarly journals PHYTOCHEMICAL AND ANTIOXIDANT COMPOSITION IN ACCESSIONS OF THE MANGABA ACTIVE GERMPLASM BANK

2021 ◽  
Vol 34 (1) ◽  
pp. 228-235
Author(s):  
PRISCILLA SANTANA SANTOS ◽  
LISIANE DOS SANTOS FREITAS ◽  
EVANDRO NEVES MUNIZ ◽  
JOSEFA GRASIELA SILVA SANTANA ◽  
ANA VERUSKA CRUZ DA SILVA
Keyword(s):  
Principal Component Analysis ◽  
Antioxidant Activity ◽  
High Performance Liquid Chromatography ◽  
High Performance ◽  
Antioxidant Properties ◽  
Principal Component ◽  
Total Phenols ◽  
Germplasm Bank ◽  
Fruit Species ◽  
Hancornia Speciosa

ABSTRACT Mangaba (Hancornia speciosa Gomes) is a fruit species native to Brazil, belonging to the Apocynaceae family. Its cultivation is predominantly extractivist and its antioxidant properties have been recently reported. The objective of this study was to evaluate the bioactive compounds and antioxidant activity in fruits of accession from the Active Germplasm Bank of Embrapa Coastal Tablelands (BI, CA, LG, PR, PT and TC). The extracts were analyzed by high-performance liquid chromatography (HPLC), which identified chlorogenic acid (93.71 - 131.66 mg.100g-1), ferulic acid (0.85 - 2.27 mg.100g-1) and rutin (238.59 -442.94 mg.100g-1). The accessions CA and PR showed the highest values of total phenols (1179.39 and 1167.05 mg GAE.100g-1, respectively). The accessions CA and TC had the highest concentration of the major compound rutin (436.78 and 442.94 mg.100g-1, respectively). Antioxidant activity values ranged from 125.95 to 158.67 g.g-1 DPPH. Principal component analysis grouped the accessions into four groups, due to the genetic variability verified in previous studies. The results will be useful to guide actions of selection and future breeding program of the species.

scholarly journals Production of eugenol from fungal endophytesNeopestalotiopsissp. andDiaporthesp. isolated fromCinnamomum loureiroileaves

PeerJ ◽  
2019 ◽  
Vol 7 ◽  
pp. e6427 ◽  
Author(s):  
Chutima Tanapichatsakul ◽  
Sarunpron Khruengsai ◽  
Sakon Monggoot ◽  
Patcharee Pripdeevech
Keyword(s):  
Antimicrobial Activity ◽  
Antioxidant Activity ◽  
Endophytic Fungi ◽  
Antioxidant Activities ◽  
Chemical Constituents ◽  
Fungal Endophytes ◽  
Biologically Active ◽  
Crude Extracts ◽  
Antioxidant Agents ◽  
Fungal Extracts

Endophytic fungi, which colonize within a host plant without causing any apparent diseases, have been considered as an important source of bioactive secondary metabolites containing antimicrobial and antioxidant activities. The aim of this research was to isolate the endophytic fungi ofCinnamomum loureiroiand then to screen their antimicrobial and antioxidant activities. A total of 11 fungal endophytes were isolated from healthy leaves ofCinnamomum loureiroibelonging to six genera:Botryosphaeria,Colletotrichum,Diaporthe,Fusarium,Neopestalotiopsis, andPestalotiopsis. All isolated strains were cultured and further extracted with ethyl acetate solvent. Antimicrobial activity of all crude endophytic fungal extracts was analyzed using disc diffusion assay against six bacterial and two fungal pathogens. Crude extracts of strains MFLUCC15-1130 and MFLUCC15-1131 showed broad-spectrum antimicrobial activity against all tested pathogens. Activity againstBacillus cereusandStaphylococcus epidermidiswas notable, showing the lowest minimum inhibitory concentration at 3.91 μg/mL. Antioxidant activity of all crude endophytic fungal extracts was also evaluated based on 2,2-diphenyl-1-picrylhydrazyl assay. Significant antioxidant activity was detected in the crude extracts of fungus MFLUCC15-1130 and MFLUCC15-1131 with IC50of 22.92 ± 0.67 and 37.61 ± 0.49 μg/mL, respectively. Using molecular identification, MFLUCC15-1130 and MFLUCC15-1131 were identified asNeopestalotiopsissp. andDiaporthesp., respectively. The major chemical constituents produced by both crude extracts were identified by gas chromatography-mass spectrometry. Eugenol, myristaldehyde, lauric acid, and caprylic acid were the primary antimicrobial and antioxidant compounds in both crude extracts. This is the first report of eugenol being a biologically active compound ofNeopestalotiopsissp. andDiaporthesp. fungal endophytes. Eugenol has been reported as antimicrobial and antioxidant agents with agronomic applications. Thus the two newly-isolated endophytes may be used for eugenol production, which in turn can be used in a variety of applications.

scholarly journals PHYTOCHEMICAL ANALYSIS AND ANTIBACTERIAL AND ANTIOXIDANT PROPERTIES OF INDIGOFERA TINCTORIA L.

2018 ◽  
Vol 11 (6) ◽  
pp. 136
Author(s):  
Chandrasekaran Swaminathan
Keyword(s):  
Antioxidant Activity ◽  
Free Radical ◽  
Antioxidant Properties ◽  
Radical Scavenging ◽  
Diffusion Method ◽  
Phytochemical Analysis ◽  
Leaf Extracts ◽  
Root Extracts ◽  
Wide Range ◽  
Indigofera Tinctoria

Objective: The present study was designed to evaluate the phytochemical composition and antibacterial and antioxidant potential of methanolic leaf and root extracts of Indigofera tinctoria L.Methods: Phytochemical analysis was done using standard methods. The methanolic leaf and root extracts of the plant were tested against Staphylococcus aureus, Enterococcus faecalis, Klebsiella pneumoniae, Enterobacter aerogenes, and Salmonella paratyphi B by cup-plate agar diffusion method. The free radical scavenging activities of the methanol extracts of leaves and roots were evaluated by 2,2-diphenyl-1-picrylhydrazyl (DPPH) assay.Results: Phytochemical screening revealed the presence of carbohydrates, reducing sugars, alkaloids, saponins, phenolic compounds, and flavonoids in methanolic leaf and root extracts. Methanolic leaf extracts of the plant exhibited antibacterial activity against a wide range of bacteria, but the root extracts failed to inhibit the tested bacterial pathogens. The antioxidant activity determination revealed that at 100 μg/ml, methanolic root extracts had the highest antioxidant activity (89.10%) on DPPH free radicals followed by methanolic leaf extracts (46.74%).Conclusion: The results of the present study conclude that the studied plant possesses broad-spectrum antibacterial and antioxidant properties and may act as a potent antioxidant for biological systems susceptible to free radical-mediated reactions. 

Antioxidant properties of Galium verum L. (Rubiaceae) extracts

2010 ◽  
Vol 5 (3) ◽  
pp. 331-337 ◽  
Author(s):  
Neda Lakić ◽  
Neda Mimica-Dukić ◽  
Jelena Isak ◽  
Biljana Božin
Keyword(s):  
Antioxidant Activity ◽  
Corn Oil ◽  
Antioxidant Properties ◽  
Radical Scavenging ◽  
Model Systems ◽  
Protective Effects ◽  
Dry Extract ◽  
Scavenging Capacity ◽  
Scavenger Activity ◽  
Dried Extract

AbstractThe antioxidant properties of methanol extracts of Lady’s Bedstraw (Galium verum L., Rubiaceae) herb from two different localities in Serbia were evaluated. Antioxidant activity was assessed in four different model systems. Free radical scavenging capacity (RSC) was examined by measuring the scavenging activity of extracts on 2,2-diphenyl-1-pycrylhydrazil (DPPH) and hydroxyl radical (OH), as well as on hydrogen peroxide. In addition, the protective effects of lipid peroxidation (LP) in corn oil were evaluated by the TBA-assay using the Fe2+/ascorbate system of induction. The amount of dried extract, the content of total phenolics, flavonoids and chlorophylls was also determined. Extracts from both locations expressed very strong scavenger activity, reducing the DPPH⊙ (IC50=3.10 µg/mland 8.04 µg/ml) and OH radical formation (IC50=0.05 µg/ml and 0.54 µg/ml) and neutralising H2O2 (IC50=4.98 µg/ml and 3.80 µg/ml), in a dose dependant manner. Also, examined extracts showed notable inhibition of LP (IC50=11.69 µg/ml and 19.47 µg/ml). The observed differences in antioxidant activity could be partially explained by the levels of phenolics (2.44–4.65 mg and 4.57–5.16 mg gallic acid equivalents/g dry extract), flavonoids (6.38–10.70 µg and 15.56–17.96 µg quercetin equivalents/g dry extract) and chlorophylls in the investigated Lady’s Bedstraw extracts.

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