Effect of mast cell–derived mediators and mast cell–related neutral proteases on human dermal fibroblast proliferation and type I collagen production

We previously demonstrated that the non-calcemic pregnacalciferol (pD) analog 17,20S (OH)2pD suppressed TGF-β1-induced type I collagen production in cultured normal human dermal fibroblasts. In the present studies, we examined fibroblasts cultured from the lesional skin of patients with systemic sclerosis (scleroderma (SSc)) and assessed the effects of 17,20S(OH)2pD on fibrosis-related mediators. Dermal fibroblast lines were established from skin biopsies from patients with SSc and healthy controls. Fibroblasts were cultured with either 17,20S(OH)2pD or 1,25(OH)2D3 (positive control) with/without TGF-β1 stimulation and extracted for protein and/or mRNA for collagen synthesis and mediators of fibrosis (MMP-1, TIMP-1, PAI-1, BMP-7, PGES, GLI1, and GLI2). 1 7,20S(OH)2pD (similar to 1,25(OH)2D3) significantly suppressed net total collagen production in TGF-β1-stimulated normal donor fibroblast cultures and in cultures of SSc dermal fibroblasts. 17,20S(OH)2pD (similar to 1,25(OH)2D3) also increased MMP-1, BMP-7, and PGES and decreased TIMP-1 and PAI1 expression in SSc fibroblasts. Although 17,20S(OH)2pD had no effect on Gli1 or Gli2 in SSc fibroblasts, it increased Gli2 expression when cultured with TGF-β1 in normal fibroblasts. These studies demonstrated that 17,20S(OH)2pD modulates mediators of fibrosis to favor the reduction of fibrosis and may offer new noncalcemic secosteroidal therapeutic approaches for treating SSc and fibrosis.

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A bioactive collagen-β tricalcium phosphate scaffold for tissue engineering

Open Life Sciences ◽

10.2478/s11535-006-0005-7 ◽

2006 ◽

Vol 1 (1) ◽

pp. 61-72 ◽

Cited By ~ 7

Author(s):

Elena Oprita ◽

Lucia Moldovan ◽

Oana Craciunescu ◽

Wanda Buzgariu ◽

Christu Tardei ◽

...

Keyword(s):

Tricalcium Phosphate ◽

Type I Collagen ◽

Dermal Fibroblast ◽

Human Dermal Fibroblast ◽

Type I ◽

X Ray Diffraction ◽

Bioactive Materials ◽

In Vitro Biocompatibility ◽

And Migration

AbstractCollagen-phosphate composites (COL/β-TCP) are novel materials that have the potential to be used as bone analogues. The aim of our study was to develop a porous bioactive material composed of type I collagen, the main bone protein and tricalcium phosphate, the mineral phase of natural bone, and investigate their in vitro biocompatibility in a human dermal fibroblast culture system. In order to obtain the bioactive materials, type I collagen was isolated from bovine tendon and characterized by physicochemical methods. β-TCP was obtained from calcium carbonate by thermal decomposition at 900 °C temperature. The powder was examined with X-ray diffraction. Two variants of COL/β-TCP scaffolds (P1 and P2) were prepared and examined by scanning electron microscopy. Our results revealed a microporous structure with small white aggregates of β-TCP, non-homogenous scattered in the collagen framework without any preferential orientation. The biocompatibility of the obtained scaffolds was tested by biochemical and histological methods on human fibroblast cultures. Both materials acted as good subtrates for human dermal fibroblast proliferation and migration.

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Inhibition of systemic sclerosis dermal fibroblast type I collagen production and gene expression by simvastatin

Arthritis & Rheumatism ◽

10.1002/art.21723 ◽

2006 ◽

Vol 54 (4) ◽

pp. 1298-1308 ◽

Cited By ~ 33

Author(s):

Natalia Louneva ◽

Gonzalo Huaman ◽

Joanna Fertala ◽

Sergio A. Jiménez

Keyword(s):

Gene Expression ◽

Systemic Sclerosis ◽

Type I Collagen ◽

Dermal Fibroblast ◽

Type I ◽

Collagen Production

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Effect of Fish Collagen Hydrolysates on Type I Collagen mRNA Levels of Human Dermal Fibroblast Culture

Marine Drugs ◽

10.3390/md16050144 ◽

2018 ◽

Vol 16 (5) ◽

pp. 144 ◽

Cited By ~ 7

Author(s):

Ana Sanchez ◽

Maria Blanco ◽

Begoña Correa ◽

Ricardo I. Perez-Martin ◽

Carmen Sotelo

Keyword(s):

Type I Collagen ◽

Dermal Fibroblast ◽

Human Dermal Fibroblast ◽

Fibroblast Culture ◽

Type I ◽

Mrna Levels ◽

Collagen Mrna ◽

Fish Collagen ◽

Collagen Hydrolysates

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Acetylated Resveratrol and Oxyresveratrol Suppress UVB-Induced MMP-1 Expression in Human Dermal Fibroblasts

Antioxidants ◽

10.3390/antiox10081252 ◽

2021 ◽

Vol 10 (8) ◽

pp. 1252

Author(s):

Jae-Eun Lee ◽

Jijeong Oh ◽

Daeun Song ◽

Mijeong Lee ◽

Dongyup Hahn ◽

...

Keyword(s):

Molecular Mechanisms ◽

Type I Collagen ◽

Dermal Fibroblast ◽

Aging Effect ◽

Inhibitory Effect ◽

Human Dermal Fibroblast ◽

Skin Aging ◽

Dermal Fibroblasts ◽

Type I ◽

Hdf Cells

Resveratrol (RES) and oxyresveratrol (OXYRES) are considered and utilized as active ingredients of anti-aging skin cosmetics. However, these compounds are susceptible to oxidative discoloration and unpleasant odor in solutions, limiting their use in cosmetics. Accordingly, RES and OXYRES were chemically modified to acetylated derivatives with enhanced stability, and their anti-aging effect on the skin and detailed molecular mechanism of their acetylated derivatives were investigated. Acetylated RES and OXYRES lost their acetyl group and exerted an inhibitory effect on H2O2-induced ROS levels in human dermal fibroblast (HDF) cells. In addition, RES, OXYRES, and their acetylated derivatives suppressed UVB-induced matrix metalloproteinase (MMP)-1 expression via inhibition of mitogen-activated protein kinases (MAPKs) and Akt/mTOR signaling pathways. Furthermore, RES, OXYRES, and their acetylated derivatives suppressed type I collagen in TPA-treated HDF cells. Collectively, these results suggest the beneficial effects and underlying molecular mechanisms of RES, OXYRES, and their acetylated derivatives for anti- skin aging applications.

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