Effect of varying the proportion of molasses in the diet on intake, digestion and microbial protein production by steers

2015 ◽  
Vol 55 (1) ◽  
pp. 17 ◽  
Author(s):  
D. V. Tuyen ◽  
X. M. Tolosa ◽  
D. P. Poppi ◽  
S. R. McLennan
Keyword(s):  
Protein Production ◽  
Volatile Fatty Acids ◽  
Rumen Fluid ◽  
Latin Square ◽  
Ammonia Nitrogen ◽  
Microbial Protein ◽  
Urine Ph ◽  
Plasma Urea ◽  
Outflow Rate ◽  
High Level

The present experiment was conducted to determine the efficiency of microbial protein production in the rumen and intake by cattle fed high-molasses diets. Intake and microbial crude protein (MCP) production were measured along with the concentration of rumen ammonia-nitrogen (N) and volatile fatty acids (VFA), pH and the rate of digestion of roughage in the rumen. Eight Brahman crossbred steers weighing 211 ± 19.3 (± s.d.) kg were used in a double 4 × 4 Latin square design. Steers were allocated to one of four total mixed rations: control (pangola hay only), 25M (25% molasses/urea mix + 75% hay), 50M (50% molasses/urea + 50% hay), and 75M (75% molasses/urea + 25% hay). The production and efficiency of production of MCP (EMCP) of the diet increased quadratically as the level of molasses in the diet increased. The EMCP from the molasses/urea mix was estimated as 166 g MCP/kg digestible organic matter (DOM), a relatively high value. Intake of dry matter (DM) and DOM increased quadratically, reaching a peak when molasses was ~50% (as fed) of the ration. Digestibility of DM increased quadratically and that of neutral detergent fibre decreased linearly with increasing level of molasses in the diet. Molasses inclusion in the diet had no effect on rumen pH, ammonia and VFA concentration in the rumen fluid, plasma urea-N, urine pH or ruminal fractional outflow rate of ytterbium-labelled particles and Cr-EDTA. It was concluded that a diet with a high level of molasses (>50%) and supplemented with adequate N had high EMCP, and that low MCP production was not a factor limiting intake or performance of cattle consuming high-molasses diets.

The effect of lipid sources on intake, rumen fermentation parameters and microbial protein synthesis in Nellore steers supplemented with glycerol

2014 ◽  
Vol 54 (10) ◽  
pp. 1871 ◽  
Author(s):  
Arturo Samuel Gomez Insuasti ◽  
Yury Tatiana Granja Salcedo ◽  
Pablo de Souza Castagnino ◽  
Bruno Ramalho Vieira ◽  
Euclides Braga Malheiros ◽  
...  
Keyword(s):  
Fatty Acids ◽  
Volatile Fatty Acids ◽  
Rumen Fluid ◽  
Latin Square ◽  
Ammonia Nitrogen ◽  
Rumen Fermentation ◽  
Ether Extract ◽  
Microbial Protein ◽  
Molar Proportion ◽  
Fermentation Parameters

The effects of glycerol with fat sources as a feed alternative were investigated in the ration for Nellore steers (Bos indicus). Eight cannulated steers at 30 months of age with initial bodyweight of 554 ± 36.0 kg were used in a double 4 by 4 Latin square design with four consecutive 16-day periods. Treatments were three different diets with lipid sources (soybean, soybean oil and calcium salts of fatty acids) and one diet control without lipid sources. All diets formulated contained 10% crude glycerol and 5% ether extract with a forage:concentrate ratio of 35:65; corn silage was used as forage. The ruminal liquid was sampled for 24 h and ruminal fermentations were monitored by measuring pH, concentrations of ammonia nitrogenand volatile fatty acids in rumen fluid. Urine samples were obtained from the total collection for 24 h for estimation of rumen microbial protein supply using urinary purine derivatives. Our results showed that the use of lipid sources combined with glycerol did not induce significant changes in rumen pH, acetate molar proportion, ruminal microbial protein or dry matter intake. Although the acetate molar proportion was kept constant within normal parameters, the propionate molar proportion was increased by the diet containing lipid sources. Moreover, we found that there was a negative effect of lipid sources on crude protein and neutral detergent fibre corrected for ash and protein intake, and one positive effect on ether extract intake. We believe that association between fat and glycerol may affect rumen fermentation parameters through reducing fibre intake and increasing propionate production and ammonia nitrogen.

scholarly journals Using glycerin with chitosan extracted from shrimp residue to enhance rumen fermentation and feed use in native Thai bulls

2021 ◽  
pp. 1158-1164
Author(s):  
Anuthida Seankamsorn ◽  
Anusorn Cherdthong ◽  
Sarong So ◽  
Metha Wanapat
Keyword(s):  
Volatile Fatty Acids ◽  
Latin Square ◽  
Ammonia Nitrogen ◽  
Rumen Fermentation ◽  
Nutrient Digestibility ◽  
Crude Glycerin ◽  
Ruminal Fermentation ◽  
Design Factor ◽  
Ch4 Production ◽  
High Level

Background and Aim: Crude glycerin is changed to propionate in the rumen, while chitosan can be used as a feed supplement to increase propionic acid concentration and decrease methane (CH4) production. We hypothesized that supplementation with a combination of a high level of crude glycerin with chitosan could have a beneficial effect on ruminal fermentation and mitigate CH4 production. This study aimed to explore the combined effects of crude glycerin and chitosan supplementation on nutrient digestibility, rumen fermentation, and CH4 calculation in native Thai bulls. Materials and Methods: Four 2-year-old native Thai bulls, weighing 150±20 kg, were kept in a 2×2 factorial arrangement in a 4×4 Latin square design. Factor A represented the incorporation of crude glycerin at 10.5% and 21% of the dry matter (DM) of a total mixed ration (TMR), and factor B represented the supplementation of chitosan at 1% and 2% DM of a TMR. Results: Increasing levels of crude glycerin at 21% decreased DM intake by 0.62 kg/day compared with 10.5% crude glycerin (p<0.05), whereas nutrient digestibility did not change (p>0.05). The incorporated crude glycerin and supplemented chitosan levels did not affect the pH, temperature, concentrations of ammonia-nitrogen, microbial population, and blood urea nitrogen (p>0.05). Supplemented chitosan and incorporated crude glycerin did not show any interaction effects on the molar portions and total volatile fatty acids (VFAs), except estimated CH4. Increasing the incorporated crude glycerin levels increased propionate and decreased the ratio of acetate to propionate ratio, whereas levels of butyrate, acetate, and total VFAs were unchanged. The combination of crude glycerin at 21% in the TMR with chitosan at 2% reduced CH4 estimation by 5.08% compared with the other feed treatment. Conclusion: Increasing incorporated crude glycerin levels in a TMR significantly elevated the propionate concentration, whereas combining 21% crude glycerin in the TMR diet with 2% chitosan supplementation could depress CH4 estimation more effectively than adding one of these supplements alone.

A comparison of tall fescue and orchardgrass silages for lactating cows

1993 ◽  
Vol 73 (4) ◽  
pp. 907-914 ◽  
Author(s):  
L. J. Fisher ◽  
S. Bittman ◽  
D. E. Hunt ◽  
J.A. Shelford ◽  
B. D. Mason
Keyword(s):  
Tall Fescue ◽  
Festuca Arundinacea ◽  
Volatile Fatty Acids ◽  
Rumen Fluid ◽  
Latin Square ◽  
Plasma Urea ◽  
Lactating Cows ◽  
Boot Stage ◽  
Molar Percent ◽  
Preference Trial

The feeding value of tall fescue silage (Festuca arundinacea schreb.) harvested at the boot stage of maturity with a crude protein (CP) content of 15.4% was compared to that of orchardgrass silage (Dactylis glomerata) harvested at the same stage of maturity with a CP content of 12.5%. Two feeding trials with lactating cows were used in this comparison. The first trial employed 10 cows in a 2 × 2 Latin- square, with treatment period 35 d in length. In the second experiment five cows were used in a preference trial where individual feed intakes, duration of feeding, and rate of forage consumption were monitored electronically for a period of 9 d.In the first trial, the cows consumed more tall fescue than orchardgrass silage (P < 0.05) (14.08 vs. 12.90 kg d−1 dry matter (DM)) but there were no differences between cows fed the two silages in milk yield (30.4 vs. 29.8 kg d−1) or milk compositon. Source of silage did not influence rumen fluid pH, molar percent of volatile fatty acids or plasma glucose; however, plasma urea-N levels were significantly higher (P < 0.05) (17.00 vs. 15.14 mg dL−1) when cows were fed tall fescue silage compared with when they were fed orchardgrass silage. In the preference trial employing electronically monitored forage feeding gates, all five cows demonstrated a preference for the tall fescue silage consuming a daily average of 9.83 kg DM for tall fescue compared with 3.73 kg for orchardgrass silage. Cows spent more time (P < 0.05) (68.3 vs. 14.1 min−1) and ate faster (69.6 vs. 54.0 g DM min−1) from the gates offering tall fescue silage compared with the gates offering orchardgrass silage. From the results of these two trials it was concluded that tall fescue silage was comparable to orchardgrass in supporting milk production and superior to orchardgrass in terms of palatability. Key words: Tall fescue, silage preference, lactating cows

The effect of supplementing ammonia-treated straw with sugar beet pulp or barley on rumen kinetics and microbial protein production

1992 ◽  
Vol 1992 ◽  
pp. 216-217
Author(s):  
S. A. Abdulrazak ◽  
X. B. Chen ◽  
E. R. Ørskov
Keyword(s):  
Sugar Beet ◽  
Protein Production ◽  
Control Diet ◽  
Latin Square ◽  
Sugar Beet Pulp ◽  
Microbial Protein ◽  
Fresh Weight Basis ◽  
Digestible Organic Matter ◽  
Outflow Rate ◽  
Beet Pulp

Data reported in the literature indicate that the efficiency of microbial protein production to ruminants vary considerably, i.e. 14-49 g microbial N/kg digestible organic matter apparently fermented in the rumen (ARC, 1984). This variation could be due to the effects of several factors, e.g. different types of feeds, rumen digesta outflow rate. The present experiment was conducted to examine whether there were differences between types of carbohydrate feedstuffs, e.g. ammonia-treated straw, barley and sugar beet pulp, as energy sources for the synthesis of microbial protein in sheep.Four Blackface x Suffolk wethers (body weight 39-42 kg) fitted with a rumen cannula were used. The animals were housed in metabolic cages and were offered 800 g/day of ammonia-treated straw (control) with or without supplementation with sugar beet pulp or barley at 20%, and 40% on fresh weight basis (SBP20, SBP40, B20 and B40 respectively). A 4 x 5 latin square design was used. The control diet also contained 20 g urea, 20 g casein, 3.4 g Na2SO4 and 10 g mineral & vitamin mixture per kg DM. Its N and ash contents were 25.6 and 74.1 g/kg DM respectively.

scholarly journals Quantitative studies of food protein degradation and the energetic efficiency of microbial protein synthesis in the rumen of sheep given chopped lucerne and rolled barley

1981 ◽  
Vol 45 (3) ◽  
pp. 587-604 ◽  
Author(s):  
J. C. Mathers ◽  
E. L. Miller
Keyword(s):  
Protein Synthesis ◽  
Rumen Fluid ◽  
Block Design ◽  
Ammonia Nitrogen ◽  
Energetic Efficiency ◽  
Food Protein ◽  
Microbial Protein ◽  
Microbial Protein Synthesis ◽  
Randomized Block Design ◽  
Outflow Rate

1. In a randomized block design, four sheep were given 800 g daily of diets containing: chopped lucerne (L), chopped lucerne–rolled barley (2:1; LB), rolled barley–chopped lucerne (2:1; BL), rolled barley (B); each diet was supplemented with minerals, vitamins and urea as considered necessary. Chromic oxide was included in the diets as a flow marker.2. Flows of organic matter (OM) and non-ammonia-nitrogen (NAN) to the small intestine (SI) were measured and microbial protein was identified by a35S-incorporation procedure.3. OM disappearance in the rumen increased linearly with increasing inclusion of barley in the diet but there was no significant change in microbial NAN flow to the SI so that the yield of microbial NAN (g)/kg fermented OM (FOM) decreased from 29.6 (diet L) to 22.7 (diet B). Changes in the energetic efficiency of microbial protein synthesis appeared to be unrelated to alterations in rumen fluid volatile fatty acid (VFA) proportions or in rumen fluid dilution rate (D).4. The degradability of dietary protein (non-urea-N), estimated using the35S procedure, was 0.72, 0.76, 0.86 and 0.86 for diets L, LB, BL and B respectively. Similar values were obtained from concurrent polyester-bag experiments when the fractional outflow rate of undergraded protein from the rumen (k) was assumed to be 0.046.

Synthesis of microbial protein in the rumen. II. A response to higher volatile fatty acids

1970 ◽  
Vol 21 (2) ◽  
pp. 297 ◽  
Author(s):  
ID Hume
Keyword(s):  
Fatty Acids ◽  
Acetic Acid ◽  
Protein Production ◽  
Volatile Fatty Acids ◽  
Rumen Fluid ◽  
Ruminal Fermentation ◽  
Microbial Protein ◽  
Nonprotein Nitrogen ◽  
Appreciable Increase ◽  
The Mean

When sheep were fed on a virtually protein-free purified diet adequately supplied with nonprotein nitrogen, 71 g microbial protein was produced in the rumen daily. The addition of a mixture of higher volatile fatty acids (VFA) increased protein production to 81 g daily (P < 0.05). The VFA supplement also increased the molar proportions of these acids in the rumen fluid and the flow of total nitrogen from the rumen. There were no differences in nitrogen balance associated with the supplement. There were significant variations between sheep and between periods in both protein production and the molar proportion of acetic acid in the rumen fluid. A negative correlation was found between acetic acid proportions and protein production (r = -0.62, P < 0.025). The mean yield of protein from the ruminal fermentation was 13.0 g/100 g organic matter digested in the rumen. The addition of the higher VFA did not result in any appreciable increase in the efficiency of protein production from the energy available.

UTILIZATION OF ENSILED OR ACID-TREATED HIGH-MOISTURE SHELLED CORN BY CATTLE

1973 ◽  
Vol 53 (3) ◽  
pp. 491-496 ◽  
Author(s):  
D. R. McKNIGHT ◽  
G. K. MACLEOD ◽  
J. G. BUCHANAN-SMITH ◽  
D. N. MOWAT
Keyword(s):  
Dry Matter ◽  
Volatile Fatty Acids ◽  
Rumen Fluid ◽  
Latin Square ◽  
Ammonia Nitrogen ◽  
Starch Digestibility ◽  
High Moisture ◽  
Nitrogen Levels ◽  
Matter Basis ◽  
Rumen Ammonia Nitrogen

Shelled corn of approximately 25% moisture was artificially dried (DC), ensiled (HMEC), or preserved either with 1.25% of a 60:40 mixture of acetic and propionic acids (HMAP) or with 1.00% propionic acid (HMP), and was examined for chemical and physical differences. Diets consisting of 66% corn (dry matter basis) were fed in a 4 × 4 latin square arrangement of treatments to four rumen and abomasal fistulated yearling heifers. High-moisture (HM) corn diets were more (P < 0.05) digestible in dry matter (77.4 vs. 74.2%) and energy (75.4 vs. 72.4%) than was the DC diet. Acid-treated corn diets had higher (P < 0.05) starch digestibility (95.6 vs. 91.7%) than DC diets. HMP diet was superior (P < 0.05) in starch digestibility to HMEC and significantly (P < 0.05) higher in nitrogen digestibility than was DC. Rumen ammonia nitrogen levels were higher (P < 0.05) on HMEC than on HMAP diet. Significantly (P < 0.05) higher total quantities of propionic and butyric acids in rumen fluid were observed on HMP diet and the quantity of total rumen volatile fatty acids tended to be higher on HMP than on other diets. Results suggest that HM corn diets move through the reticulorumen more slowly and experience greater digestion in this area than does DC diet.

scholarly journals Concentrate containing calcium salts of fatty acids rich in polyunsaturated fatty acids can change rumen fermentation in grazing goats

2018 ◽  
Vol 39 (6) ◽  
pp. 2621
Author(s):  
Ludmila Couto Gomes ◽  
Claudete Regina Alcalde ◽  
Julio Cesar Damasceno ◽  
Luiz Paulo Rigolon ◽  
Ana Paula Silva Possamai ◽  
...  
Keyword(s):  
Fatty Acids ◽  
Volatile Fatty Acids ◽  
Rumen Fluid ◽  
Latin Square ◽  
Rumen Fermentation ◽  
Ruminal Fermentation ◽  
Calcium Salts ◽  
Linear Effect ◽  
Concentrate Supplementation ◽  
Ruminal Ph

Feeding goats with calcium salts of fatty acids (CSFA) can supply ruminants with lipids, with minimal effects on ruminal fermentation and fiber digestibility. However, there is a shortage of information on the effect of CSFA on characteristics of rumen fermentation in grassland goats. Thus, the present study aimed to assess the addition of CSFA to concentrate on the parameters of rumen fermentation of grazing goats. Five rumen cannulated goats were distributed in a Latin square 5x5 design (treatments: 0%, 1.5%, 3.0%, 4.5% and 6.0% CSFA. The pH, ammonia N and volatile fatty acids (VFA) content were analyzed in the ruminal fluid at 0, 2, 4, 6 and 8 hours after concentrate supplementation. The pH and ammonia N concentration showed a linear effect with the addition of CSFA. There was no effect observed for the VFA molar concentration after grazing goats were fed with the experimental diet. In conclusion, further research is needed to investigate the addition of CSFA to goat diets because there is evidence that CSFA increases ruminal pH and decreases excess ruminal ammonia without changing the VFA concentration in the rumen fluid.

scholarly journals Produk Fermentasi Rumen dan Produksi Protein Mikroba Sapi Lokal yang Diberi Pakan Jerami Amoniasi dan Beberapa Bahan Pakan Sumber Energi

2011 ◽  
Vol 11 (2) ◽  
pp. 29-34 ◽  
Author(s):  
Novita Hindratiningrum ◽  
Muhamad Bata ◽  
Setya Agus Santosa
Keyword(s):  
Protein Synthesis ◽  
Rice Bran ◽  
Volatile Fatty Acids ◽  
Rumen Fluid ◽  
Gas Production ◽  
Metabolic Energy ◽  
Energy Sources ◽  
Microbial Protein ◽  
Microbial Protein Synthesis

Products of rumen fermentation and protein microbial of dairy cattle feed with rice bran ammonization and some feedstuffs as an energy sourcesABSTRACT. This study aims to examine the energy sources of feed ingredients that can increase the production of Volatile Fatty Acids (VFA), N-NH3, microbial protein synthesis, total gas production and metabolic energy. The material used is as a source of rumen fluid inoculum from Frisian Holstein cows (FH) females, amoniasi rice straw, salt, mineral mix brand "Ultra Minerals' production Eka Farma Semarang, onggok wet and dry, corn, and rice bran. Observed variable is the concentration of (VFA), N-NH3, rumen microbial protein synthesis, and total gas production. Based on the analysis of diversity seen any significant effect (P0.05) on total VFA concentration, N-NH3 and total gas but had no effect (P0.05) on microbial protein synthesis. Conclusion of research is the provision of energy sources with rice bran treatment, onggok wet and dry corn flour can be used as fermentable carbohydrates on feed hay amoniasi in vitro.

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