UTILIZATION OF ENSILED OR ACID-TREATED HIGH-MOISTURE SHELLED CORN BY CATTLE

1973 ◽  
Vol 53 (3) ◽  
pp. 491-496 ◽  
Author(s):  
D. R. McKNIGHT ◽  
G. K. MACLEOD ◽  
J. G. BUCHANAN-SMITH ◽  
D. N. MOWAT
Keyword(s):  
Dry Matter ◽  
Volatile Fatty Acids ◽  
Rumen Fluid ◽  
Latin Square ◽  
Ammonia Nitrogen ◽  
Starch Digestibility ◽  
High Moisture ◽  
Nitrogen Levels ◽  
Matter Basis ◽  
Rumen Ammonia Nitrogen

Shelled corn of approximately 25% moisture was artificially dried (DC), ensiled (HMEC), or preserved either with 1.25% of a 60:40 mixture of acetic and propionic acids (HMAP) or with 1.00% propionic acid (HMP), and was examined for chemical and physical differences. Diets consisting of 66% corn (dry matter basis) were fed in a 4 × 4 latin square arrangement of treatments to four rumen and abomasal fistulated yearling heifers. High-moisture (HM) corn diets were more (P < 0.05) digestible in dry matter (77.4 vs. 74.2%) and energy (75.4 vs. 72.4%) than was the DC diet. Acid-treated corn diets had higher (P < 0.05) starch digestibility (95.6 vs. 91.7%) than DC diets. HMP diet was superior (P < 0.05) in starch digestibility to HMEC and significantly (P < 0.05) higher in nitrogen digestibility than was DC. Rumen ammonia nitrogen levels were higher (P < 0.05) on HMEC than on HMAP diet. Significantly (P < 0.05) higher total quantities of propionic and butyric acids in rumen fluid were observed on HMP diet and the quantity of total rumen volatile fatty acids tended to be higher on HMP than on other diets. Results suggest that HM corn diets move through the reticulorumen more slowly and experience greater digestion in this area than does DC diet.

scholarly journals PSXI-21 Effects of different levels and protein sources on ruminal fermentation of feedlot Nellore steers fed high-concentrate diets

2019 ◽  
Vol 97 (Supplement_3) ◽  
pp. 405-405
Author(s):  
Vinícius C De Souza ◽  
Juliana Messana ◽  
Erick Batista ◽  
Paulo Henrique Colovate ◽  
Maria Júlia Ganga ◽  
...  
Keyword(s):  
Protein Level ◽  
Repeated Measures ◽  
Volatile Fatty Acids ◽  
Rumen Fluid ◽  
Latin Square ◽  
Ruminal Fermentation ◽  
Protein Sources ◽  
Nitrogen Levels ◽  
Protein Levels ◽  
Different Levels

Abstract The goal of this study was to evaluate the effects of different levels (10 and 13% crude protein) and protein source on ruminal fermentation of feedlot Nellore steers fed high-concentrate diets. The protein sources tested were urea, dried distillers grains (DDG) and corn gluten meal. The DDG and gluten were used as RUP sources. We used 6 cannulated Nellore steers, rumen, duodenum, and ileum arranged in 6×6 Latin square designed in factorial (2 nitrogen levels and 3 protein sources) balanced for residues. The pH was measured immediately after rumen fluid sampling at 0, 2, 4, 6, 8 and 10h after feeding. The NH3-N concentrations were determined with micro-Kjeldahl apparatus. The concentration of volatile fatty acids (VFA) was assessed by gas chromatography. Data were evaluated using the MIXED procedure of SAS, with repeated measures over time. There was no interaction between sampling time and treatments for NH3-N and pH (P &gt; 0.05). Animals fed diets containing Urea had a higher concentration of NH3-N (20.6 mg/dL) than animals supplemented with Gluten (16.2 mg/dL) or DDG (12.7 mg / dL). There was an increase in the NH3-N concentration (P = 0.049) with the increase of the protein level 10 to 13% CP with 14.7 and 18.3 mg/dL, respectively. The ruminal pH was affected by the dietary protein level (P = 0.016), in which animals fed a level of 10% CP had a higher pH (6.80) compared to the level of 13% CP (6.43). Total and individual VFA concentration was not affected by protein levels and sources (P &gt; 0.05). The reduction of N level and the use of RUP did not adversely affect the ruminal fermentation. Although significant differences were observed for pH, they were small and adequate for microbial protein synthesis in the rumen.

The effect of lipid sources on intake, rumen fermentation parameters and microbial protein synthesis in Nellore steers supplemented with glycerol

2014 ◽  
Vol 54 (10) ◽  
pp. 1871 ◽  
Author(s):  
Arturo Samuel Gomez Insuasti ◽  
Yury Tatiana Granja Salcedo ◽  
Pablo de Souza Castagnino ◽  
Bruno Ramalho Vieira ◽  
Euclides Braga Malheiros ◽  
...  
Keyword(s):  
Fatty Acids ◽  
Volatile Fatty Acids ◽  
Rumen Fluid ◽  
Latin Square ◽  
Ammonia Nitrogen ◽  
Rumen Fermentation ◽  
Ether Extract ◽  
Microbial Protein ◽  
Molar Proportion ◽  
Fermentation Parameters

The effects of glycerol with fat sources as a feed alternative were investigated in the ration for Nellore steers (Bos indicus). Eight cannulated steers at 30 months of age with initial bodyweight of 554 ± 36.0 kg were used in a double 4 by 4 Latin square design with four consecutive 16-day periods. Treatments were three different diets with lipid sources (soybean, soybean oil and calcium salts of fatty acids) and one diet control without lipid sources. All diets formulated contained 10% crude glycerol and 5% ether extract with a forage:concentrate ratio of 35:65; corn silage was used as forage. The ruminal liquid was sampled for 24 h and ruminal fermentations were monitored by measuring pH, concentrations of ammonia nitrogenand volatile fatty acids in rumen fluid. Urine samples were obtained from the total collection for 24 h for estimation of rumen microbial protein supply using urinary purine derivatives. Our results showed that the use of lipid sources combined with glycerol did not induce significant changes in rumen pH, acetate molar proportion, ruminal microbial protein or dry matter intake. Although the acetate molar proportion was kept constant within normal parameters, the propionate molar proportion was increased by the diet containing lipid sources. Moreover, we found that there was a negative effect of lipid sources on crude protein and neutral detergent fibre corrected for ash and protein intake, and one positive effect on ether extract intake. We believe that association between fat and glycerol may affect rumen fermentation parameters through reducing fibre intake and increasing propionate production and ammonia nitrogen.

AMMONIA OR UREA TREATMENT OF WHOLE HIGH MOISTURE SHELLED CORN

1981 ◽  
Vol 61 (3) ◽  
pp. 703-711 ◽  
Author(s):  
D. N. MOWAT ◽  
P. McCAUGHEY ◽  
G. K. MACLEOD
Keyword(s):  
Feed Efficiency ◽  
Dry Matter ◽  
Latin Square ◽  
Corn Silage ◽  
Starch Digestibility ◽  
Large Decrease ◽  
High Moisture ◽  
Acid Detergent Fiber ◽  
Urea Treatment ◽  
Ground Corn

Treatments of high moisture shelled corn were compared in diets containing approximately 60% (dry matter (DM) basis) corn silage. In an initial digestibility trial, treatments were: (1) ensiled, ground; (2) 1.5% (wt/wt) mixture of acetic: propionic acid, whole; (3) 2% (wt/wt DM basis) anhydrous ammonia, whole. Treatments were evaluated with six yearling steers in a double 3 × 3 latin square design. Urea was included in the ensiled and acid-treated corn diets to supply sufficient nitrogen. Ammoniating whole corn resulted in similar starch and energy digestibilities compared to ensiled ground corn. However, a large decrease in starch digestibility occurred with acid-treated whole corn. Digestibility of acid detergent fiber was greater (P < 0.05) with both ammoniated and acid-treated whole than ensiled ground corn. In a feedlot trial, two high moisture corn treatments compared were: (1) ensiled, ground; (2) 3.7% (wt/wt DM basis) urea, whole. Eighty Hereford yearling steers compared the two corn diets each with or without monensin. Temperatures rose steadily within bins plateauing approximately 21/2 wk after storage. The amount of urea that hydrolyzed to ammonia varied depending upon location within bins. A total of 1.4% DM loss occurred during storage. Urea-treatment tended to slightly reduce liveweight gain and feed efficiency. Monensin decreased intake (P < 0.05) and slightly improved feed efficiency (P > 0.05) with both types of corn. Effects of monensin on rumen VFA were similar with both corn diets. Type of corn had no effect on rumen VFA proportions.

Some effects of chloral hydrate on rumen fermentation and digestion in sheep

1982 ◽  
Vol 99 (1) ◽  
pp. 215-224 ◽  
Author(s):  
J. C. Mathers ◽  
E. L. Miller
Keyword(s):  
Fatty Acids ◽  
Dry Matter ◽  
Volatile Fatty Acids ◽  
Chloral Hydrate ◽  
Rumen Fluid ◽  
Ammonia Nitrogen ◽  
Long Chain Fatty Acids ◽  
Ether Extract ◽  
Rumen Metabolism ◽  
Long Chain

SUMMARYTwo experiments were carried out with adult sheep to investigate the effects of chloral hydrate (CH) on rumen metabolism and on the sites and extent of digestion.Experiment 1 confirmed that CH is a very potent methane inhibitor; doses of 1 or 4 g/day reduced methane production to 4% of that observed in the absence of the drug. In both experiments, CH administration altered the proportions of volatile fatty acids in rumen fluid. The main changes were an increase in propionic acid and a decrease in acetic acid with smaller increases in the proportion of isovaleric, valeric and caproic acids. Stoichiometric calculations indicated that only 21% of the hydrogen saved by prevention of methanogenesis was diverted to the synthesis of additional propionic and butyric acids and the fate of the remaining hydrogen was not determined unequivocally.CH had little effect on the extent of biohydrogenation in the rumen of the long-chain fatty acids in dried grass.The apparent digestibility of ether extract was reduced (P< 0·05) by CH but those of other proximate constituents and energy were little affected. There were no s gnificant effects of CH on the extent of digestion of dry matter, organic matter, non-ammonia nitrogen or ash between the mouth and abomasum, abomasum and terminal ileum or ileum and anus.

scholarly journals REPLACEMENT OF TIFTON 85 HAY WITH MANIÇOBA HAY IN THE SPINELESS CACTUS DIET OF SHEEP1 2

2021 ◽  
Vol 34 (1) ◽  
pp. 219-227
Author(s):  
FRANCICLEIDE MARIA DE SOUZA CHARLL SANTOS ◽  
DORGIVAL MORAIS DE LIMA JÚNIOR ◽  
DANIEL BARROS CARDOSO ◽  
MICHEL DO VALE MACIEL ◽  
FRANCISCO FERNANDO RAMOS DE CARVALHO
Keyword(s):  
Nutrient Intake ◽  
Crude Protein ◽  
Dry Matter ◽  
Volatile Fatty Acids ◽  
Latin Square ◽  
Protein Digestibility ◽  
Ammonia Nitrogen ◽  
Fatty Acid Production ◽  
Insoluble Fibre ◽  
Four Levels

ABSTRACT The objective of this study was to evaluate the effect of replacing Tifton 85 hay with maniçoba hay in diets based on spineless cactus on the nutrient intake and digestibility, ingestive behaviour and ruminal parameters of confined sheep. In order to do this, eight male sheep with ruminal cannulae were used, distributed across four levels (0, 333, 666 and 1.000 g kg-1 of dry matter) of replacement of Tifton 85 hay with maniçoba hay in a double Latin square experimental design. The animals were confined for 60 days divided into four periods of 15 days. The replacement of Tifton 85 hay with maniçoba hay did not influence the dry matter intake or digestibility. However, it influenced in a positive linear fashion the intake of non-fibrous carbohydrates and in a linear negative fashion the digestibility of insoluble fibre in neutral detergent and crude protein. The ruminal ammonia-nitrogen and the crude protein ruminal content decreased linearly with the replacement of Tifton 85 hay with maniçoba hay. There was no effect of hay replacement on the production of volatile fatty acids or the microbial biofilm. The total replacement Tifton 85 by maniçoba hay in spineless cactus diets for sheep did not influence total digestible nutrient intake, volatile fatty acid production or biofilm, but did affect crude protein digestibility, ammonia-nitrogen and crude protein content in the rumen. Therefore, maniçoba hay can replace 300 g kg-1 grass hay in spineless cactus diets for sheep without compromising intake and ruminal parameters.

scholarly journals Supplementation levels for non-lactating cows grazing during the rainy season

2017 ◽  
Vol 38 (2) ◽  
pp. 885
Author(s):  
Joelson Antonio Silva ◽  
Luciano Da Silva Cabral ◽  
Daniel De Paula Sousa ◽  
André Soares de Oliveira ◽  
Rosemary Lais Galati ◽  
...  
Keyword(s):  
Dairy Cows ◽  
Rainy Season ◽  
Dry Matter ◽  
Rumen Fluid ◽  
Nitrogen Concentration ◽  
Latin Square ◽  
Ammonia Nitrogen ◽  
Dry Matter Intake ◽  
Panicum Maximum ◽  
Grazing Dairy Cows

This study aimed to evaluate levels of concentrate supplementation (1.5, 3.0, 4.5 and 6.0 kg day?1) for grazing dairy cows kept on Panicum maximum Jacq. cv. Tanzania pasture during the rainy season on nutrient intake and digestibility and rumen parameters. Four rumen cannulated non-lactating Holstein × Zebu crossbred cows were used in this study by a 4X4 Latin square design, which presented an average initial body weight of 521.69±31.98 kg. Each period lasted 17 days, being the first 10 days were used for animal adaptation and the remaining seven days for data collection. There was no effect (P > 0.05) of supplementation levels on total dry matter intake, although forage dry matter intake has been linearly decreased (P < 0.05). Treatments have no effect (P > 0.05) on the digestibility coefficients of dry matter, organic matter, crude protein, or ether extract. There was a linear increase (P < 0.05) on daily rumination time and total rumination times according to supplementation levels. There was no significant effect of supplementation levels (P > 0.05) or time after supplementation on rumen pH. Rumen ammonia nitrogen concentration responded quadratically (P < 0.05) to times after supplementation, with a maximum estimated concentration of 17.61 mg dL?1 at 3.87 h after supplementation. Increasing supplementation levels for grazing dairy cows reduces forage intake but has no negative effects on total dry matter intake or rumen-fluid pH.

Effect of varying the proportion of molasses in the diet on intake, digestion and microbial protein production by steers

2015 ◽  
Vol 55 (1) ◽  
pp. 17 ◽  
Author(s):  
D. V. Tuyen ◽  
X. M. Tolosa ◽  
D. P. Poppi ◽  
S. R. McLennan
Keyword(s):  
Protein Production ◽  
Volatile Fatty Acids ◽  
Rumen Fluid ◽  
Latin Square ◽  
Ammonia Nitrogen ◽  
Microbial Protein ◽  
Urine Ph ◽  
Plasma Urea ◽  
Outflow Rate ◽  
High Level

The present experiment was conducted to determine the efficiency of microbial protein production in the rumen and intake by cattle fed high-molasses diets. Intake and microbial crude protein (MCP) production were measured along with the concentration of rumen ammonia-nitrogen (N) and volatile fatty acids (VFA), pH and the rate of digestion of roughage in the rumen. Eight Brahman crossbred steers weighing 211 ± 19.3 (± s.d.) kg were used in a double 4 × 4 Latin square design. Steers were allocated to one of four total mixed rations: control (pangola hay only), 25M (25% molasses/urea mix + 75% hay), 50M (50% molasses/urea + 50% hay), and 75M (75% molasses/urea + 25% hay). The production and efficiency of production of MCP (EMCP) of the diet increased quadratically as the level of molasses in the diet increased. The EMCP from the molasses/urea mix was estimated as 166 g MCP/kg digestible organic matter (DOM), a relatively high value. Intake of dry matter (DM) and DOM increased quadratically, reaching a peak when molasses was ~50% (as fed) of the ration. Digestibility of DM increased quadratically and that of neutral detergent fibre decreased linearly with increasing level of molasses in the diet. Molasses inclusion in the diet had no effect on rumen pH, ammonia and VFA concentration in the rumen fluid, plasma urea-N, urine pH or ruminal fractional outflow rate of ytterbium-labelled particles and Cr-EDTA. It was concluded that a diet with a high level of molasses (>50%) and supplemented with adequate N had high EMCP, and that low MCP production was not a factor limiting intake or performance of cattle consuming high-molasses diets.

Effect of dietary monensin and tetronasin on rumen fermentation in sheep

1990 ◽  
Vol 1990 ◽  
pp. 31-31
Author(s):  
C. J. Newbold ◽  
R. J. Wallace
Keyword(s):  
Dry Matter ◽  
Volatile Fatty Acids ◽  
Rumen Fluid ◽  
Control Diet ◽  
Latin Square ◽  
Rumen Fermentation ◽  
Mixed Diet ◽  
Feed Conversion ◽  
Nutritional Effects

Tetronasin is an ionophore which improves feed conversion efficiency in ruminants (Bartleet al, 1988). Its nutritional effects are at least partly derived from its influence on nitrogen and energy metabolism in the rumen. The mode of action of tetronasin is therefore similar to monensin and although it is more potent than monensin, it has a similar spectrum of antimicrobial activity (Newboldet al, 1988). The aim of the present study was to compare the effects of the inclusion of tetronasin or monensin in the diet on the rumen fermentation.Three mature sheep weighing approximately 50 kg and fitted with rumen cannulae were fed 1 kg/d of a mixed diet of hay, barley, molasses, fishmeal and vitamins/minerals (500, 299.5, 100, 91 and 9.5 g/kg dry matter respectively) in two equal meals. A control diet (no addition, C), tetronasin (10 ppm in the diet, T) or monensin (33 ppm, M) were compared in a 3 x 3 Latin square. Periods were 28 days long with samples taken on two consecutive days during the last 5 days. Samples of rumen fluid were withdrawn at 0, 1, 2, 4 and 6 h after the morning feed for the determination of pH, volatile fatty acids (VFA) L-lactic acid and ammonia concentrations.

Ruminal flora studies. VIII. The influence of rate and method of feeding a ration upon its digestibility, upon ruminal function, and upon the ruminal population

1957 ◽  
Vol 8 (3) ◽  
pp. 254 ◽  
Author(s):  
RJ Moir ◽  
M Somers
Keyword(s):  
Volatile Fatty Acid ◽  
Dry Matter ◽  
Latin Square ◽  
Nitrogen Retention ◽  
Ammonia Nitrogen ◽  
Daily Ration ◽  
Distinctive Pattern ◽  
Nitrogen Levels ◽  
Ph Values ◽  
Dry Matter Digestibility

A single ration made up of commercial sheep cubes (20 per cent. protein) together with oaten and lucerne chaff was fed to five sheep in five different ways. A Latin square design was used. Measurements were made on the ruminal population, the metabolism within the rumen, and the overall nitrogen balance and dry matter digestibility. Each of the different treatments resulted in a distinctive pattern in the ruminal population, pH, ammonia nitrogen, and volatile fatty acid levels. There were significant differences in dry matter digestibility and nitrogen retention. The daily ration given as a single feed gave the lowest dry matter digestibility and nitrogen retention. It also caused the greatest fall in pH values and bacterial counts, and the lowest protozoal counts. The highest ruminal ammonia nitrogen levels were also associated with this treatment. The best performance was obtained by feeding the concentrate in the morning and roughage in the afternoon, or feeding the whole ration in four equal portions at 2-hr intervals. The other two treatments – half ration morning and afternoon, or chaff in the morning and concentrates in the afternoon – gave intermediate results. Some implications of the various data are discussed, and the strong influence of feeding pattern is stressed.

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