Incidence of virus infection in experimental plots, commercial crops, and seed stocks of cool season crop legumes

Experimental plots of cool season crop legumes growing at diverse locations in Western Australia were inspected for plants with suspect virus symptoms over 4 growing seasons (1994, 1997, 1998, 1999), and plant samples were tested for infection with alfalfa mosaic (AMV), bean yellow mosaic (BYMV), cucumber mosaic (CMV), and pea seed-borne mosaic (PSbMV) viruses. All 4 viruses were detected in faba bean (Vicia faba); BYMV, CMV, and PSbMV in field pea (Pisum sativum); AMV, CMV, and PSbMV in lentil (Lens culinaris); and AMV and CMV in chickpea (Cicer arietinum). Among minor crop species, AMV, BYMV, and CMV were found in narbon bean (V. narbonensis) and grass pea (Lathyrus sativus); BYMV and CMV in dwarf chickling (L. cicera); BYMV in bitter vetch (V. e r v i l i a ) and L. clymenum; and AMV in fenugreek (Trigonella foenum-graecum). Incidences of individual viruses varied widely from site to site but plot infection sometimes reached 100%. Symptom severity varied widely with virus–crop combination. In large-scale surveys of commercial crops of field pea and faba bean over 2 (1998, 1999) and 3 (1994, 1998, 1999) growing seasons, respectively, randomly collected samples from each crop were tested for presence of AMV, BYMV, CMV, and PSbMV. In 1999 they were also tested for beet western yellows virus (BWYV). All 5 viruses were detected in both species. BWYV was found in 35% of faba bean and 56% of the field pea crops sampled in 1999, with incidences of infection in individual crops up to 40% and 49%, respectively. PSbMV was found in 42% and BYMV in 18% of field pea crops in 1999. In individual crops, highest infection incidences of BYMV and PSbMV detected were 31% for BYMV in faba bean in 1998 and 9% for PSbMV in field pea in 1999. CMV and AMV incidences in both species never exceeded 7% of crops or 4% of plants within individual crops. Infection by 2 different viruses within individual crops was common, even 3 were sometimes found. Cultivars infected with most viruses were Fiesta and Fiord for faba bean, and Dundale, Laura, and Magnet for field pea. BYMV was detected in the crop tested of dwarf chickling. In tests on seed samples from Western Australia of 30 commercial seed stocks of field pea, 11 of faba bean, and 50 of chickpea, PSbMV was detected in 11, 1, and 1, respectively; CMV in 1, 1, and 3; BYMV in 3, 1, and 0; and AMV in 0, 0, and 1. This appears to be the first record of seed transmission of CMV in pea and faba bean. Seed samples from Victoria were also found to contain viruses: PSbMV in pea and AMV in lentil. Widespread infection with viruses in evaluation plots and commercial crops of cool season crop legumes is a cause for concern, especially where individual crop incidences are high and 2 or more viruses are present. Sowing of infected seed stocks leads to introduction of randomly dispersed sources of virus infection within the crop sown, resulting in spread of infection and yield losses. Appropriate control measures are discussed.

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An evaluation of potential Rhizobium inoculant strains used for pulse production in acidic soils of south-east Australia

Australian Journal of Experimental Agriculture ◽

10.1071/ea03129 ◽

2005 ◽

Vol 45 (3) ◽

pp. 257 ◽

Cited By ~ 15

Author(s):

J. Evans

Keyword(s):

Nitrogen Fixation ◽

Relative Humidity ◽

Western Australia ◽

Faba Bean ◽

Field Experiments ◽

Collaborative Study ◽

Field Pea ◽

Acidic Soil ◽

Wide Range ◽

Inoculant Strain

Profitability of the pulse industry relies considerably on crop nitrogen fixation because this process supplies greater than 60% of pulse crop nitrogen. Therefore the industry requires the most efficient Rhizobium symbioses and effective inoculation management. Re-appraisal of the recommended inoculant strain for field pea, SU303, in south-east Australia, was warranted by field evidence that SU303 failed to maximise grain yield at sites in Western Australia. Re-appraisal of the inoculant strain for faba bean and lentil, WSM1274, was warranted because of anecdotal evidence from Western Australia of associated crop failures. In addition, a glasshouse study in Western Australia reported greater dry matter production by faba bean and lentil inoculated with strains other than WSM1274. This paper reports trials comparing potential inoculant strains for field pea and faba bean in soils of south-east Australia. Comparisons are based on efficiency for nitrogen fixation, survival on seed and survival in soil. Additionally, because the pulse industry lacked comprehensive information to assist decision making on the need for recurring inoculation, relevant investigation of this issue is also reported. The results of 3 field experiments for efficiency for nitrogen fixation, over mildly (pHCa 5.0) to strongly (pHCa 4.3) acidic soil in south-east Australia supported replacing SU303 as the commercial inoculant. The efficiency for nitrogen fixation of WSM1274 on faba bean was not found to be inferior to alternative strains. However, its capacity for survival on seed at temperatures of 15°C and above, over a wide range of relative humidity, and perhaps its capacity for survival in acidic soil, was inferior. This provided additional evidence to justify the replacement of this inoculant strain that was agreed to by a national steering committee in 2001, based on the Western Australia reports, the early experiments in this study and those of a collaborative study in Victoria. Alternative inoculant strains to SU303 and WSM1274 were identified in the current study. Temperature and relative humidity conditions suitable for maintaining inoculant viability with extended storage of inoculated field pea and faba bean are also discussed. A survey of rhizobia surviving in soil was used to determine the time scale of persistence of Rhizobium leguminosarum bv. viciae and Bradyrhizobium sp. (Lupinus) in soils of the south-east. It was concluded that in soils of pH (CaCl2) <5.1, inoculation of field pea and faba bean should be routinely practiced; none of the strains of R. leguminosarum bv. viciae tested showed ability for survival in strongly acidic soil sufficient to obviate seed inoculation. It was further concluded that the absence of a legume host for lupin rhizobia for 4 or more years would also warrant reintroducing inoculant of B. sp. (Lupinus).

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Yield losses caused by virus infection in four combinations of non-persistently aphid-transmitted virus and cool-season crop legume

Australian Journal of Experimental Agriculture ◽

10.1071/ea03060 ◽

2004 ◽

Vol 44 (1) ◽

pp. 57 ◽

Cited By ~ 14

Author(s):

L. J. Latham ◽

R. A. C. Jones ◽

B. A. Coutts

Keyword(s):

Virus Infection ◽

Mosaic Virus ◽

Seed Yield ◽

Faba Bean ◽

Seed Weight ◽

Dry Weight ◽

Yield Losses ◽

Cool Season ◽

Individual Seed ◽

Shoot Dry Weight

Field experiments provided quantitative information on the yield losses caused by virus infection within 4 different combinations of non-persistently aphid-transmitted virus and cool-season crop legume: Alfalfa mosaic virus (AMV) in chickpea, faba bean and lentil, and Cucumber mosaic virus (CMV) in lentil. Virus infection foci were introduced into plots and naturally occurring aphids spread infection from these to the other plants. Plants were tagged individually when typical virus symptoms first appeared during the growing period. Paired plant comparisons between symptomatic and asymptomatic plants were made to measure different yield loss parameters. Late infection with AMV in faba bean cv. Fiord diminished shoot dry weight by 41% and seed yield by 45%, but plants infected earlier recovered sufficiently from their initial shock reaction not to produce significant yield losses. In plants of lentil cv. Matilda first showing symptoms at different times, infection with AMV decreased shoot dry weight by 74–76%, seed yield by 81–87% and individual seed weight by 10–21%, while CMV diminished shoot dry weight by 72–81%, seed yield by 80–90% and individual seed yield by 17–25%. Early infection with AMV killed plants of chickpea cv. Tyson while later infection decreased shoot dry weight by 50%, seed yield by 98% and individual seed weight by 90%. The first tentative evidence for seed transmission of AMV in faba bean is reported with a transmission rate of 0.04%.

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First Report of Faba bean necrotic yellows virus Affecting Legume Crops in Azerbaijan

Plant Disease ◽

10.1094/pdis-93-11-1220c ◽

2009 ◽

Vol 93 (11) ◽

pp. 1220-1220 ◽

Cited By ~ 14

Author(s):

S. G. Kumari ◽

N. Attar ◽

E. Mustafayev ◽

Z. Akparov

Keyword(s):

Faba Bean ◽

Subterranean Clover ◽

First Record ◽

Field Pea ◽

Economic Losses ◽

Growing Seasons ◽

Subterranean Clover Stunt Virus ◽

Legume Crops ◽

Primer Sets ◽

Legume Production

A total of 482 chickpea (Cicer arietinum L.), 182 lentil (Lens culinaris Medik.), 12 vetch (Vicia sativa L.), 5 field pea (Pisum sativum L.), and 3 faba bean (Vicia faba L.) samples were collected from plants with symptoms suggestive of a viral infection (leaf rolling, yellowing, and stunting) from the major legume-production areas of Azerbaijan in the 2007 and 2008 growing seasons. All samples were tested by the tissue-blot immunoassay (3) at the Virology Laboratory of ICARDA, Syria using 11 specific legume virus antisera including a monoclonal antibody (2-5H9) (1) for Faba bean necrotic yellows virus (FBNYV). Laboratory tests showed that FBNYV was detected in 73, 61, 11, 3, and 2 samples of chickpea, lentil, vetch, field pea, and faba bean, respectively. Total DNA was extracted from six FBNYV-positive samples (two chickpea, two lentil, and two vetch) and tested by PCR with the following four primer sets (FBNYV, Milk vetch dwarf virus [MDV], Subterranean clover stunt virus [SCSV], and nanovirus DNA-R primers [F103 and R101]) (2). All six Azeri samples as well as the reference nanovirus isolates (SCSV-Australia, MDV-Japan, and FBNYV-Syria) generated amplicons of the expected size (~770 bp) using the nanovirus DNA-R primers (F103 & R101). In addition, Azeri samples and FBNYV-Syria yielded a PCR amplicon of the expected size (666 bp) with the FBNYV primer pair. The MDV- and SCSV-specific primers did not generate amplicons with these six samples. Sequence analysis of the FBNYV amplicons from two isolates (AzL 282-07 from lentil [GenBank Accession No. GQ351600] and AzV 277-07 from vetch [GenBank Accession No. GQ371215]) showed that they were 99% identical with each other. Comparing the sequence of AzL 282-07 with that of other nanoviruses revealed identities of 97% (FBNYV-Spain; DQ830990), 96% (FBNYV-Iran; AM493900), 92% (FBNYV-Syria; Y11408), 92% (FBNYV-Egypt; AJ132183), 78% (MDV; AB044387) and 69% (SCSV-Australia; U16734). FBNYV has been reported to infect food legumes in many countries in West Asia and North Africa and cause economic losses on faba bean in Egypt, Jordan, and Syria. To our knowledge, this is the first record of FBNYV infecting legume crops in Azerbaijan. References: (1) A. Franz et al. Ann. Appl. Biol. 128:255, 1996. (2) S. G. Kumari et al. Phytopathol. Mediterr. 47:42, 2008. (3) K. M. Makkouk and A. Comeau. Eur. J. Plant Pathol. 100:71, 1994.

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Further studies on Pea seed-borne mosaic virus in cool-season crop legumes: responses to infection and seed quality defects

Australian Journal of Agricultural Research ◽

10.1071/ar08113 ◽

2008 ◽

Vol 59 (12) ◽

pp. 1130 ◽

Cited By ~ 28

Author(s):

B. A. Coutts ◽

R. T. Prince ◽

R. A. C. Jones

Keyword(s):

Mosaic Virus ◽

Seed Coat ◽

Faba Bean ◽

Seed Quality ◽

Broad Bean ◽

Seed Transmission ◽

Field Pea ◽

Cool Season ◽

Moderately Resistant ◽

Pea Seed

Field and glasshouse experiments (3 of each) were done during 2003–06 to determine the responses of a range of genotypes belonging to 13 species of cool-season crop legumes to infection with Pea seed-borne mosaic virus (PSbMV). Seed quality defects were determined and genotypes of some species were also tested for seed transmission of the virus. In field experiments, of 39 genotypes of field pea (Pisum sativum) evaluated, 15 were ranked as highly susceptible, 10 susceptible, 9 moderately resistant, and 5 resistant, while all 7 lupin species (Lupinus spp.) tested were resistant. In glasshouse sap and graft inoculations with PSbMV to genotypes not found infected in the field and 2 additional lupin species, no virus was detected in any of the 9 lupin species or in 5 field pea genotypes tested. Thus, the lupins all appeared to be non-hosts and the 5 field pea genotypes had resistance to the 2 PSbMV isolates used to inoculate them. All 14 genotypes of faba bean (Vicia faba) evaluated in the field were ranked highly susceptible, while 12 out of 16 lentil (Lens culinaris) genotypes were ranked as highly susceptible and 4 as susceptible. Chickpea (Cicer arietinum) genotypes were moderately resistant (50) or susceptible (7). Once infected, plant sensitivities (symptom severities) ranged from low in some field pea and most lentil genotypes to high in most faba bean genotypes. Chickpea genotypes all were ranked as moderately sensitive. Seed lots harvested from PSbMV-infected plants of field pea, faba bean, and chickpea all showed severe seed quality defects, but lentil was usually less affected. The predominant seed symptoms were necrotic rings and line markings on the seed coat, malformation, reduced size, and splitting. Kabuli chickpea types also showed darkening of the seed coat. Seed transmission of PSbMV was detected in faba bean (0.2%) and field pea (5–30%). When PSbMV infection foci were introduced into plots of lentil cv. Nugget, the virus spread to the lentil plants and decreased shoot dry weight by 23%, seed yield by 96%, and individual seed weight by 58%. Seed transmission of PSbMV (6%) was detected in seed from the infected lentil plants. In a survey for possible viral seed symptoms, all seed lots of kabuli chickpea (5) and field pea (70), and 10 of 18 of faba bean were affected, but none of the 23 of lentil. When seedlings from 16 faba bean and 7 field pea seed lots were tested for 3 viruses, neither Broad bean stain virus nor Broad bean true mosaic virus was detected, but PSbMV was found in 5 field pea seed lots at incidences of <1–14%. PSbMV was detected in commercial field pea seed stocks of cvv. Kaspa (33) and Parafield (12) at incidences of 0.5–47% and 0.3–30%, respectively. The implications of these findings in terms of genotype susceptiblility and sensitivity to PSbMV infection and their importance for the management of PSbMV in legume crops are discussed.

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Grain yield and cadmium concentration of a range of grain legume species grown on two soil types at Merredin, Western Australia

Australian Journal of Experimental Agriculture ◽

10.1071/ea03137 ◽

2005 ◽

Vol 45 (9) ◽

pp. 1167 ◽

Cited By ~ 1

Author(s):

R. F. Brennan ◽

R. J. French

Keyword(s):

Western Australia ◽

Faba Bean ◽

Cadmium Concentration ◽

Grain Legumes ◽

Field Pea ◽

Lupinus Luteus ◽

Soil Types ◽

Grain Legume ◽

Red Clay ◽

Yellow Lupin

Five grain legumes species, narrow-leafed lupin (Lupinus angustifolius L.), field pea (Pisum sativum L.), faba bean (Vicia faba L.), chickpea (Cicer arietinum L.), and yellow lupin (Lupinus luteus L.), were grown on 2 soil types, a red clay and red duplex soil, in the < 400 mm rainfall district of Western Australia. The study showed that chickpea, field pea and faba bean accumulated less cadmium (Cd) in dried shoots and grain than narrow-leafed lupin. Yellow lupin had Cd concentrations ~3 times higher in dried shoots and ~9 times higher in grain than narrow-leafed lupin. For both experiments, the ranking (lowest to highest) of mean Cd concentration (mg Cd/kg) in the grain was: chickpea (0.017) < field pea (0.024) = faba bean (0.024) < narrow-leafed lupin (0.033) < yellow lupin (0.300).

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A comparison of seed yields of winter grain legumes in Western Australia

Australian Journal of Experimental Agriculture ◽

10.1071/ea9930915 ◽

1993 ◽

Vol 33 (7) ◽

pp. 915 ◽

Cited By ~ 52

Author(s):

KHM Siddique ◽

GH Walton ◽

M Seymour

Keyword(s):

Seed Yield ◽

Western Australia ◽

Faba Bean ◽

Dry Matter ◽

Black Spot ◽

Field Pea ◽

Grain Legume ◽

Alkaline Soils ◽

Narrow Leaf ◽

Seed Yields

Field trials were conducted in 2 seasons at 13 sites on neutral to alkaline soils in Western Australia, to compare the growth and seed yield of 6 winter grain legume species: field pea (Pisum sativum L.), chickpea (Cicer arietinum L.), faba bean (Vicia faba L.), lentil (Lens culinaris Medik), narrow leaf lupin (Lupinus angustifolius L.), albus lupin (L. albus). In a dry year (1991), overall site mean seed yield was highest for field pea (1.35 t/ha), then faba bean (1.22 t/ha) and narrow leaf lupin (0.85 t/ha). Chickpea, lentil line ILL5728, and albus lupin produced an average seed yield of 0.64 t/ha. Rainfall in 1992 was above average and seed yields of all species except field pea were higher than in 1991. Heavy rainfall in winter and spring caused transient waterlogging at several sites, affecting growth and seed yield of most species. Faba bean responded positively to the increase in rainfall and produced exceptional seed yields of >4 t/ha at 3 sites. Mean seed yield was highest for faba bean, at 2.87 t/ha, then narrow leaf lupin (1.19 t/ha), chickpea (1.1 t/ha), and field pea (1.0 t/ha). Field pea performed poorly at several sites due to its susceptibility to transient waterlogging and black spot disease (caused by Mycosphaerella pinoides). Albus lupin and lentil line ILL5728 produced similar seed yields (0.78 t/ha). Lentil cvv. Laird (1991) and Kye (1992) had low seed yields due to poor adaptation. Seed yield differences between species at various locations were not simply related to any soil chemical parameters or to depth to clay. On a calcareous soil of pH(CaC12) 8 at Dongara, the growth of narrow leaf lupin was severely affected and the crop failed. Days to flowering varied between species; faba bean was earliest to flower (76 days), then field pea. Faba bean and field pea (particularly in 1991) generally produced the most dry matter, both early and at final harvest. The relationship between seed yield and rainfall was complicated by transient waterlogging and fungal disease (e.g. black spot in field pea) at many sites. Seed yield was significantly positively related to final dry matter production but not to harvest index.

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Faba Bean and Pea Can Provide Late-Fall Forage Grazing without Affecting Maize Yield the Following Season

Agronomy ◽

10.3390/agronomy10010080 ◽

2020 ◽

Vol 10 (1) ◽

pp. 80 ◽

Cited By ~ 1

Author(s):

Bryce J. Andersen ◽

Dulan P. Samarappuli ◽

Abbey Wick ◽

Marisol T. Berti

Keyword(s):

Faba Bean ◽

Cover Crops ◽

Cover Crop ◽

Bos Taurus ◽

Farming Systems ◽

Maize Yield ◽

Triticum Aestivum L ◽

Field Pea ◽

Upper Midwest ◽

Crop Species

Faba bean (Vicia faba Roth) and pea (Pisum sativum L.) are grown worldwide as protein sources for food and feed and can be used as cover crops after wheat (Triticum aestivum L.). However, faba bean is underutilized in upper Midwest farming systems. This study was conducted to determine how faba bean relates to pea as a forage, cover crop, and in cycling of nutrients to maize (Zea mays L.) in the following season. Five faba bean cultivars and two pea cultivars, a forage pea and a field pea, were established after wheat harvest in North Dakota, in 2017 and 2018. Faba bean and pea cultivars averaged 1.3 Mg ha−1 of biomass, enough to support 1.5 animal unit month (AUM) ha−1 for a 450 kg cow (Bos taurus L.) with calf, at 50% harvest efficiency. Crude protein content was highest in faba bean cv. Boxer (304 g kg−1), with faba bean cv. Laura and forage pea cv. Arvika having similar content, and field pea having the least (264 g kg−1). Cover crop treatments did not affect maize in the following year, indicating no nutrient cycling from faba bean and pea to maize. Both cover crop species tested provided high protein forage, suitable for late grazing, with a more fibrous crop residue. Faba bean has potential as a cover crop in the upper Midwest while providing greater quality forage than pea.

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Assessment of quality of life in patients with the effects of transient ischemic stroke

East European Journal of Neurology ◽

10.33444/2411-5797.2016.6(12).37-39 ◽

2016 ◽

pp. 37-39

Author(s):

A. Babirad

Keyword(s):

Quality Of Life ◽

Ischemic Stroke ◽

Large Scale ◽

Cerebrovascular Diseases ◽

Control Measures ◽

Sociological Research ◽

Cerebrovascular Events ◽

Sf 36 ◽

Angioplasty And Stenting

Cerebrovascular diseases are a problem of the world today, and according to the forecast, the problem of the near future arises. The main risk factors for the development of ischemic disorders of the cerebral circulation include oblique and aging, arterial hypertension, smoking, diabetes mellitus and heart disease. An effective strategy for the prevention of cerebrovascular events is based on the implementation of large-scale risk control measures, including the use of antiagregant and anticoagulant therapy, invasive interventions such as atheromectomy, angioplasty and stenting. In this connection, the efforts of neurologists, cardiologists, angiosurgery, endocrinologists and other specialists are the basis for achieving an acceptable clinical outcome. A review of the SF-36 method for assessing the quality of life in patients with the effects of transient ischemic stroke is presented. The assessment of quality of life is recognized in world medical practice and research, an indicator that is also used to assess the quality of the health system and in general sociological research.

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