Anatomy, histochemistry and phytochemical profile of leaf and stem bark of Bathysa cuspidata (Rubiaceae)

2012 ◽  
Vol 60 (1) ◽  
pp. 49 ◽  
Author(s):  
Victor Peçanha de Miranda Coelho ◽  
João Paulo Viana Leite ◽  
Líria Granato Nunes ◽  
Marília Contin Ventrella

Bathysa cuspidata (A. St. Hil.) Hook. f. ex K. Schum. is a species native to the Atlantic Rainforest of Brazil, with stem bark currently used in folk medicine. Uncontrolled bark collections cause problems of environmental sustainability, making it necessary to evaluate more abundant and renewable new sources of raw plant material, such as leaves. This work evaluated the anatomy, histochemistry and phytochemistry of leaves and stem bark of B. cuspidata, aiming to find similarities in the chemical composition between these plant parts and to provide anatomical data to supplement the quality control of this plant drug. Common light and scanning electron microscopy techniques were used in the anatomical and histochemical studies. Thin-layer chromatography and high-performance liquid chromatography were used for the phytochemistry analysis. The concentration of total phenolics was determined using the Folin–Ciocalteau method and the contents of proanthocyanidins using the vanillin reagent. The leaves and bark of B. cuspidata display morphoanatomical characters common to the family Rubiaceae. The histochemical and phytochemical results reveal similarities and differences between the leaves and bark of B. cuspidata, suggesting that the extracts of both parts should be considered and subjected to pharmacological studies in order to investigate the effectiveness of the therapeutic actions reported in folk medicine.

Author(s):  
RIDHI JOSHI ◽  
RISHIKESH MEENA ◽  
PREETI MISHRA ◽  
VIDYA PATNI

Objective: A normal-phase high-performance thin-layer chromatography (HPTLC) method has been developed and validated for estimation and quantitation of beta-sitosterol from the methanolic fraction of different plant parts of two medicinally important plants viz. Merremia aegyptia and Merremia dissecta. These plants have been reported to possess antimicrobial, antioxidant, and anti-inflammatory activities. Methods: Chromatographic separation of beta-sitosterol from the methanolic extracts of plant parts of M. aegyptia and M. dissecta was performed on TLC aluminum plates pre-coated with silica gel 60F254 using a suitable mobile phase. The densitometric scanning was done after derivatization at ????-580 nm for ????-sitosterol. Result: Only M. dissecta leaf sample was reported to contain ????-sitosterol (4.6 ng/μl), whereas other samples such as seed, stem, and callus extracts of M. aegyptia and M. dissecta did not showed its presence. Conclusion: The developed HPTLC method is simple, rapid, and precise and can be used for routine analysis and quantification of ????-sitosterol and other useful plant bioactives that are phytopharmaceutically important.


2022 ◽  
pp. 698-710
Author(s):  
Neha Mishra ◽  
Rashmi Srivastava

Cinnamon has been used as a spice, condiment, and aromatic plant since centuries ago. Cinnamon is a small evergreen tree belonging to the genus Cinnamomum in the family Lauraceae. There are more than 250 species of cinnamon worldwide. In India, Cinnamomum verum and Cinnamomum cassia are the most common species grown in the Himalaya region. They have been used as folk medicine for the treatment of nausea, flatulent dyspepsia, coughs, diarrhea, malaria, gastric disorder, and to alleviate pain and inflammation in rheumatic arthritis. Therapeutic properties of cinnamon are due to the presence of bioactive constituents such as p-coumaric, cinnamaldehyde, cinnamic acid, and eugenol. Cinnamaldehyde and eugenol are the major active constituents responsible for its characteristic flavor, aroma, and therapeutic properties. Pharmacological studies found that it could be a promising candidate with potential for designing new drugs. This review is aimed to summarize the ethanomedicinal importance, phytochemistry, and wide spectrum of pharmacological and therapeutic applications of cinnamon.


Plants ◽  
2019 ◽  
Vol 8 (6) ◽  
pp. 149 ◽  
Author(s):  
Atul Kabra ◽  
Natália Martins ◽  
Rohit Sharma ◽  
Ruchika Kabra ◽  
Uttam Singh Baghel

Myrica esculenta (Myricaceae) is a popular medicinal plant most commonly found in the sub-tropical Himalayas. It is widely used in folk medicine to treat several ailments such as asthma, cough, chronic bronchitis, ulcers, inflammation, anemia, fever, diarrhea, and ear, nose, and throat disorders. Due to its multidimensional pharmacological and therapeutic effects, it is well recognized in the ayurvedic pharmacopeia. However, the recent upsurge in M. esculenta use and demand has led to illicit harvesting by the horticultural trade and habitat loss, pushing the plant to the brink of extinction. Thus, the present review aims to provide updated information on M. esculenta botany, ethnomedicinal uses, phytochemistry, pharmacological effects, toxicity, and conservation methods, as well as also highlight prospective for future research. Particular emphasis is also given to its antioxidant potential in health promotion. In-depth literature was probed by searching several sources via online databases, texts, websites, and thesis. About 57 compounds were isolated and identified from M. esculenta, and the available reports on physicochemical parameters, nutritional and high-performance thin-layer chromatography analysis of bioactive plant parts are portrayed in a comparative manner. Friendly holistic conservation approaches offered by plant biotechnology applications, such as micropropagation, germplasm preservation, synthetic seed production, and hairy root technologies are also discussed. Nonetheless, further studies are needed to propose the mechanistic role of crude extracts and other bioactives, and even to explore the structure–function relationship of active components.


2014 ◽  
Vol 86 (3) ◽  
pp. 1167-1179 ◽  
Author(s):  
MARIA OLÍVIA MERCADANTE-SIMÕES ◽  
HELLEN C. MAZZOTTINI-DOS-SANTOS ◽  
LAYS A. NERY ◽  
PERACIO R.B. FERREIRA ◽  
LEONARDO M. RIBEIRO ◽  
...  

The bark of the underground stem of Tontelea micrantha (Mart. ex. Schult.) A. C. Sm., a native Brazilian Cerrado species, is used in folk medicine for treating kidney ailments. The structures of the underground and the aerial stems were examined and their barks were analyzed for the presence of secondary metabolites. Bark fragments were processed according to conventional techniques in plant anatomy and their chemical compositions examined using histochemical and phytochemical tests, thin layer chromatography, and high-efficiency liquid chromatography. The underground stem is a sobol with unusual cambial activity. Laticifers that secrete terpenoids were present in the cortex and phloem of both organs and can contribute to the identification of the species in field. Druses were present in both barks, but mono-crystals were only observed in the sobol. Tannins, flavonoids, alkaloids, and terpenoids occurred in both types of bark, but carotenoids were only detected in the sobol. The similarities between these two organs indicate that the aerial stem bark has potential medicinal use and represents a plausible alternative to harvesting the sobol, which could contribute to the preservation of natural populations of this species.


2016 ◽  
Vol 22 (3) ◽  
pp. 413-428 ◽  
Author(s):  
Surasak Saokaew ◽  
Preyanate Wilairat ◽  
Paranya Raktanyakan ◽  
Piyameth Dilokthornsakul ◽  
Teerapon Dhippayom ◽  
...  

Kaempferia parviflora (Krachaidum) is a medicinal plant in the family Zingiberaceae. Its rhizome has been used as folk medicine for many centuries. A number of pharmacological studies of Krachaidum had claimed benefits for various ailments. Therefore, this study aimed to systematically search and summarize the clinical evidences of Krachaidum in all identified indications. Of 683 records identified, 7 studies were included. From current clinical trials, Krachaidum showed positive benefits but remained inconclusive since small studies were included. Even though results found that Krachaidum significantly increased hand grip strength and enhanced sexual erotic stimuli, these were based on only 2 studies and 1 study, respectively. With regard to harmful effects, we found no adverse events reported even when Krachaidum 1.35 g/day was used. Therefore, future studies of Krachaidum are needed with regards to both safety and efficacy outcomes.


2014 ◽  
Vol 2 (04) ◽  
pp. 09-14
Author(s):  
Prasanth Dharmarajan ◽  
Harisha . ◽  
Raiby Paul ◽  
Anup B. Thakar ◽  
Switu Jani

Background: Panchavalkala Choorna is a poly herbal compound made from the barks of five major drugs from the Ficus family. It is widely quoted as an effective remedy in various skin disorders explained under the wide spectrum of Kushta (skin disorders), mentioned in Ayurvedic classics. However, till date no published data is available on the analytical profile of this compound formulation. The present study was aimed to standardize and develop the pharmacognostical and phytochemical profile of Panchavalkala Choorna. The compund was prepared as per classical methods and analytical findings were systematically recorded. The samples were subjected to organoleptic analysis, physicochemical analysis and High performance Thin Layer Chromatography (HPTLC) examination by optimizing the solvent systems. Pharmacognostical profile of Panchavalkala Choorna was established. Loss on drying, Specific gravity, Viscosity and Refractive index, Iodine value, Acid value and Saponification value of Panchavalkala Choorna were found within prescribed limits. HPTLC fingerprinting profile of ST revealed 6 spots at 254 nm and 4 spots at 366 nm. Two spots were merging in both the long and short UV, showing common characters in both the wavelengths.


Author(s):  
Neha Mishra ◽  
Rashmi Srivastava

Cinnamon has been used as a spice, condiment, and aromatic plant since centuries ago. Cinnamon is a small evergreen tree belonging to the genus Cinnamomum in the family Lauraceae. There are more than 250 species of cinnamon worldwide. In India, Cinnamomum verum and Cinnamomum cassia are the most common species grown in the Himalaya region. They have been used as folk medicine for the treatment of nausea, flatulent dyspepsia, coughs, diarrhea, malaria, gastric disorder, and to alleviate pain and inflammation in rheumatic arthritis. Therapeutic properties of cinnamon are due to the presence of bioactive constituents such as p-coumaric, cinnamaldehyde, cinnamic acid, and eugenol. Cinnamaldehyde and eugenol are the major active constituents responsible for its characteristic flavor, aroma, and therapeutic properties. Pharmacological studies found that it could be a promising candidate with potential for designing new drugs. This review is aimed to summarize the ethanomedicinal importance, phytochemistry, and wide spectrum of pharmacological and therapeutic applications of cinnamon.


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