Molecular mapping of a novel early leaf-senescence gene Els2 in common wheat by SNP genotyping arrays

2020 ◽  
Vol 71 (4) ◽  
pp. 356 ◽  
Author(s):  
N. Wang ◽  
Y. Z. Xie ◽  
Y. Z. Li ◽  
S. N. Wu ◽  
H. S. Wei ◽  
...  
Keyword(s):  
Ssr Markers ◽  
Common Wheat ◽  
Leaf Senescence ◽  
Molecular Mapping ◽  
Brachypodium Distachyon ◽  
Nuclear Gene ◽  
Limiting Factors ◽  
Yield Potential ◽  
Starting Point ◽  
Early Leaf Senescence

Early leaf senescence in wheat (Triticum aestivum L.) is one of the limiting factors for developing high yield potential. In this study, a stably inherited, early leaf-senescence mutant LF2099 was initially identified in an M2 population of the common wheat accession H261 after ethyl methanesulfonate (EMS) mutagenesis. Early leaf senescence was observed in the LF2099 mutant during the three-leaf-stage, and then the etiolated area of the wheat leaf increased gradually from the bottom to the top throughout development. Compared with H261, the chlorophyll (Chl a, Chl b) and carotenoid contents and photosynthetic capacity of the mutant were significantly decreased. All of its yield-related traits except for spike length were also significantly reduced. Dissolved cytoplasm, abnormal chloroplast structure, dissolved chloroplast membrane, abnormal thylakoid development, and more plastoglobules were observed in the senescent leaf region of the mutant by transmission electronic microscope. Genetic analysis indicated that the early leaf-senescence phenotype is controlled by an incomplete-dominance nuclear gene, here designated Els2. Using single nucleotide polymorphisms and bulked segregant analysis, the els2 gene was anchored in a region on chromosome 2BL between simple sequence repeat (SSR) markers gpw4043 and wmc149. Six new polymorphic SSR markers were developed from the Chinese Spring 2BL shotgun survey sequence contigs. By means of comparative genomics analyses, the collinearity genomic regions of the els2 locus on wheat 2BL were identified in Brachypodium distachyon chromosome 5, rice (Oryza sativa) chromosome 4 and sorghum (Sorghum bicolor) chromosome 6. Five intron polymorphism (IP) markers were further developed from this collinearity genomic region. Ultimately, Els2 was mapped in a genetic interval of 0.95 cM flanked by IP markers 2BIP09 and 2BIP14. The co-segregating IP markers 2BIP12 and 2BIP17 provide a starting point for the fine mapping and map-based cloning of Els2.

scholarly journals Genetic Diversity of Selected Rice Genotypes under Water Stress Conditions

Plants ◽  
2020 ◽  
Vol 10 (1) ◽  
pp. 27
Author(s):  
Mahmoud M. Gaballah ◽  
Azza M. Metwally ◽  
Milan Skalicky ◽  
Mohamed M. Hassan ◽  
Marian Brestic ◽  
...  
Keyword(s):  
Genetic Diversity ◽  
Water Stress ◽  
Drought Stress ◽  
Drought Tolerance ◽  
Ssr Markers ◽  
Similarity Index ◽  
Stress Conditions ◽  
Yield Potential ◽  
Drought Tolerant ◽  
Rice Genotypes

Drought is the most challenging abiotic stress for rice production in the world. Thus, developing new rice genotype tolerance to water scarcity is one of the best strategies to achieve and maximize high yield potential with water savings. The study aims to characterize 16 rice genotypes for grain and agronomic parameters under normal and drought stress conditions, and genetic differentiation, by determining specific DNA markers related to drought tolerance using Simple Sequence Repeats (SSR) markers and grouping cultivars, establishing their genetic relationship for different traits. The experiment was conducted under irrigated (normal) and water stress conditions. Mean squares due to genotype × environment interactions were highly significant for major traits. For the number of panicles/plants, the genotypes Giza179, IET1444, Hybrid1, and Hybrid2 showed the maximum mean values. The required sterility percentage values were produced by genotypes IET1444, Giza178, Hybrid2, and Giza179, while, Sakha101, Giza179, Hybrid1, and Hybrid2 achieved the highest values of grain yield/plant. The genotypes Giza178, Giza179, Hybrid1, and Hybrid2, produced maximum values for water use efficiency. The effective number of alleles per locus ranged from 1.20 alleles to 3.0 alleles with an average of 1.28 alleles, and the He values for all SSR markers used varied from 0.94 to 1.00 with an average of 0.98. The polymorphic information content (PIC) values for the SSR were varied from 0.83 to 0.99, with an average of 0.95 along with a highly significant correlation between PIC values and the number of amplified alleles detected per locus. The highest similarity coefficient between Giza181 and Giza182 (Indica type) was observed and are susceptible to drought stress. High similarity percentage between the genotypes (japonica type; Sakha104 with Sakha102 and Sakha106 (0.45), Sakha101 with Sakha102 and Sakha106 (0.40), Sakha105 with Hybrid1 (0.40), Hybrid1 with Giza178 (0.40) and GZ1368-S-5-4 with Giza181 (0.40)) was also observed, which are also susceptible to drought stress. All genotypes are grouped into two major clusters in the dendrogram at 66% similarity based on Jaccard’s similarity index. The first cluster (A) was divided into two minor groups A1 and A2, in which A1 had two groups A1-1 and A1-2, containing drought-tolerant genotypes like IET1444, GZ1386-S-5-4 and Hybrid1. On the other hand, the A1-2 cluster divided into A1-2-1 containing Hybrid2 genotype and A1-2-2 containing Giza179 and Giza178 at coefficient 0.91, showing moderate tolerance to drought stress. The genotypes GZ1368-S-5-4, IET1444, Giza 178, and Giza179, could be included as appropriate materials for developing a drought-tolerant variety breeding program. Genetic diversity to grow new rice cultivars that combine drought tolerance with high grain yields is essential to maintaining food security.

scholarly journals Impairment in Sulfite Reductase Leads to Early Leaf Senescence in Tomato Plants

2014 ◽  
Vol 165 (4) ◽  
pp. 1505-1520 ◽  
Author(s):  
Dmitry Yarmolinsky ◽  
Galina Brychkova ◽  
Assylay Kurmanbayeva ◽  
Aizat Bekturova ◽  
Yvonne Ventura ◽  
...  
Keyword(s):  
Leaf Senescence ◽  
Sulfite Reductase ◽  
Tomato Plants ◽  
Early Leaf Senescence

scholarly journals Arabidopsis mlo3 mutant plants exhibit spontaneous callose deposition and signs of early leaf senescence

2019 ◽  
Vol 101 (1-2) ◽  
pp. 21-40 ◽  
Author(s):  
Stefan Kusch ◽  
Susanne Thiery ◽  
Anja Reinstädler ◽  
Katrin Gruner ◽  
Krzysztof Zienkiewicz ◽  
...  
Keyword(s):  
Leaf Senescence ◽  
Callose Deposition ◽  
Early Leaf Senescence

Lesson study outcomes: a theoretical model

2019 ◽  
Vol 8 (2) ◽  
pp. 117-134 ◽  
Author(s):  
John Paul Mynott
Keyword(s):  
Case Studies ◽  
Lesson Study ◽  
Design Methodology ◽  
Potential Outcomes ◽  
Limiting Factors ◽  
Educational Setting ◽  
Content Type ◽  
Starting Point ◽  
The Impact ◽  
Practical Implications

Purpose Lesson study (LS) research is disadvantaged by a lack of clarity surrounding the potential outcomes an LS cycle can produce for participant learning. The purpose of this paper is to set out a model of the potential outcomes an LS cycle can achieve. The model identifies the limitations that can occur in LS groups and how these limitations impact on the overall outcomes for participants. Design/methodology/approach Case studies are used to exemplify the different outcomes in the model taken from five years of LS work in a primary school in England. The case studies shape the four different outcomes of the model, defining and contextualising the attributes and characteristics of each outcome. Findings The model presented indicates that there are four key outcomes for LS cycles, with the most common outcome being a form of limited learning. The paper explores the limitations of time, collaboration and expertise to articulate how each of these limiting factors has a bearing on the overall outcome for an LS cycle. Research limitations/implications The model is currently based on a singular educational setting. This means that each outcome needs further exploration through wider LS work in order to clarify and refine the outcome model. Practical implications The outcome model will support the development of a shared vocabulary for discussing LS cycles. By articulating where on the outcome model an LS is, it is possible for researchers to discuss how to reduce the impact of limitations and other challenges to LS, enabling research to develop a more evaluation-led approach to using LS. Originality/value The outcome model supports LS researchers in articulating the outcomes of their LS cycles with a shared vocabulary. It addresses understudied areas of LS research, namely failed and dysfunctional LS cycles and identifies that while an LS can bring the potential for participant learning, the cycle outcomes are the starting point for participant change.

scholarly journals High-Resolution Mapping of the Novel Early Leaf Senescence Gene Els2 in Common Wheat

Plants ◽  
2020 ◽  
Vol 9 (6) ◽  
pp. 698
Author(s):  
Na Wang ◽  
Yanzhou Xie ◽  
Yingzhuang Li ◽  
Shengnan Wu ◽  
Shuxian Li ◽  
...  
Keyword(s):  
High Resolution ◽  
Leaf Senescence ◽  
Chinese Spring ◽  
Snp Array ◽  
Triticum Aestivum L ◽  
Snp Markers ◽  
F2 Population ◽  
High Resolution Map ◽  
Kasp Markers ◽  
Early Leaf Senescence

Early leaf senescence negatively impacts the grain yield in wheat (Triticum aestivum L.). Induced mutants provide an important resource for mapping and cloning of genes for early leaf senescence. In our previous study, Els2, a single incomplete dominance gene, that caused early leaf senescence phenotype in the wheat mutant LF2099, had been mapped on the long arm of chromosome 2B. The objective of this study was to develop molecular markers tightly linked to the Els2 gene and construct a high-resolution map surrounding the Els2 gene. Three tightly linked single-nucleotide polymorphism (SNP) markers were obtained from the Illumina Wheat 90K iSelect SNP genotyping array and converted to Kompetitive allele-specific polymerase chain reaction (KASP) markers. To saturate the Els2 region, the Axiom® Wheat 660K SNP array was used to screen bulked extreme phenotype DNA pools, and 9 KASP markers were developed. For fine mapping of the Els2 gene, these KASP markers and previously identified polymorphic markers were analyzed in a large F2 population of the LF2099 × Chinese Spring cross. The Els2 gene was located in a 0.24-cM genetic region flanked by the KASP markers AX-111643885 and AX-111128667, which corresponded to a physical interval of 1.61 Mb in the Chinese Spring chromosome 2BL containing 27 predicted genes with high confidence. The study laid a foundation for a map-based clone of the Els2 gene controlling the mutation phenotype and revealing the molecular regulatory mechanism of wheat leaf senescence.

scholarly journals SSR markers linked to mite (Polyphagotarsonemus latus Banks) resistance in jute (Corchorus olitorius L.)

2010 ◽  
Vol 46 (No. 2) ◽  
pp. 64-74 ◽  
Author(s):  
A. Ghosh ◽  
S. Sharmin ◽  
S. Islam ◽  
M.U. Pahloan ◽  
S. Islam ◽  
...  
Keyword(s):  
Ssr Markers ◽  
Dna Markers ◽  
Genetic Linkage ◽  
Genetic Linkage Map ◽  
Limiting Factors ◽  
Resistance Allele ◽  
Selection Efficiency ◽  
Polyphagotarsonemus Latus ◽  
Phenotypic Marker ◽  
Ssr Primers

The mite is considered as one of the major limiting factors of jute production in Bangladesh. So it is essential to select genotypes with the desired resistance allele combination using DNA markers. Eighty-eight SSR primers were used to screen the mite resistant and sensitive parents. Among them only twenty-one primer pairs showed polymorphism. A genetic linkage map was constructed using these polymorphic primers in one hundred and fifty F<sub>2</sub> populations, derived from a cross between the parents. The SSR markers J-170 and HK-89 have been mapped at 34.1 cM and 35.4 cM, respectively, on either side of the phenotypic marker. These two markers (J-170 and HK-89) along with HK-64 showed 100% selection efficiency in combination.

Comparative proteomic analysis of the maize responses to early leaf senescence induced by preventing pollination

2018 ◽  
Vol 177 ◽  
pp. 75-87 ◽  
Author(s):  
Liancheng Wu ◽  
Shunxi Wang ◽  
Lei Tian ◽  
Liuji Wu ◽  
Mingna Li ◽  
...  
Keyword(s):  
Leaf Senescence ◽  
Proteomic Analysis ◽  
Comparative Proteomic Analysis ◽  
Early Leaf Senescence

scholarly journals Characterization of Somatic Embryogenesis Receptor-Like Kinase 4 as a Negative Regulator of Leaf Senescence in Arabidopsis

Cells ◽  
2019 ◽  
Vol 8 (1) ◽  
pp. 50 ◽  
Author(s):  
Xiaoxu Li ◽  
Salman Ahmad ◽  
Akhtar Ali ◽  
Cun Guo ◽  
Hong Li ◽  
...  
Keyword(s):  
Somatic Embryogenesis ◽  
Leaf Senescence ◽  
Stress Responses ◽  
Negative Regulator ◽  
Knockout Mutant ◽  
Surface Receptors ◽  
Extracellular Signals ◽  
Early Leaf Senescence

Leaf senescence is a genetically controlled process that involves the perception of extracellular signals and signal transduction. The receptor-like protein kinases (RLKs) are known to act as an important class of cell surface receptors and are involved in multiple biological processes such as development and stress responses. The functions of a number of RLK members have been characterized in Arabidopsis and other plant species, but only a limited number of RLK proteins have been reported to be associated with leaf senescence. In the present study, we have characterized the role of the somatic embryogenesis receptor kinase 4 (SERK4) gene in leaf senescence. The expression of SERK4 was up-regulated during leaf senescence and by several abiotic stress treatments in Arabidopsis. The serk4-1 knockout mutant was found to display a significant early leaf senescence phenotype. Furthermore, the results of overexpression analysis and complementary analysis supported the idea that SERK4 acts as a negative regulator in the process of leaf senescence.

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